Abstract:
:Recombination studies of bacteria are often carried out with multicopy plasmids, and recombination frequencies are often deduced from the proportion of cells in the population that express a recombinant phenotype. These frequencies should however be called apparent frequencies, since detection of the recombinant cells requires not only the formation of a rearranged plasmid but also its establishment in the cell. The establishment of the recombinant plasmid can possibly be affected by its interaction with the parental plasmids. To test this hypothesis, we have used a plasmid system enabling the study of deletion formation between short direct repeats (18 bp) in Bacillus subtilis and developed a method by which deletion frequencies are measured under conditions under which interaction is abolished. Real deletion frequencies were thus determined and compared with apparent deletion frequencies. Real frequencies were underestimated by a factor ranging from 4- to 500-fold, depending upon the plasmid under study. This implies that a large majority of the recombinant molecules that are formed are generally not detected. We show that apparent deletion frequencies strongly depend upon (i) the parental plasmid copy number, (ii) the ability of the recombinant molecules to form heterodimeric plasmids, and (iii) the fitness of the recombinant molecules relative to that of parental molecules. Finally, we show that under conditions under which all recombinant molecules are scored, transcription can inhibit the deletion process 10-fold.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Chédin F,Dervyn R,Ehrlich SD,Noirot Pdoi
10.1128/jb.179.3.754-761.1997subject
Has Abstractpub_date
1997-02-01 00:00:00pages
754-61issue
3eissn
0021-9193issn
1098-5530journal_volume
179pub_type
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1972-04-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/JB.83.1.20-26.1962
更新日期:1962-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.744-753.1989
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.17.5548-5558.1993
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pub_type: 杂志文章
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更新日期:1990-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1972-11-01 00:00:00
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更新日期:2018-10-10 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1977-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.667-675.1969
更新日期:1969-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.180.8.2087-2092.1998
更新日期:1998-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.22.6451-6458.1996
更新日期:1996-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1999-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.182.17.5009-5012.2000
更新日期:2000-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.365-373.1986
更新日期:1986-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.318-320.1984
更新日期:1984-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-12-01 00:00:00
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更新日期:2003-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.96.3.706-712.1968
更新日期:1968-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.109.1.140-151.1972
更新日期:1972-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.17.4951-4958.2000
更新日期:2000-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.1.187-195.1975
更新日期:1975-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4605-4614.1993
更新日期:1993-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.1.92-101.1970
更新日期:1970-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.3.1047-1051.1980
更新日期:1980-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00220-07
更新日期:2007-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.558-561.1975
更新日期:1975-10-01 00:00:00
abstract::Quie, Paul G. (University of Minnesota, Minneapolis) and Lewis W. Wannamaker. Staphylococcal Muller phenomenon: relationship to the plasminogen-plasmin system. J. Bacteriol. 82:770-783. 1961.-The staphylococcal factor that produces particulate proteolysis (Muller phenomenon) in whole blood or hemoglobinserum agar plat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.5.770-783.1961
更新日期:1961-11-01 00:00:00