Mutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2.

Abstract:

:A novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important polymer when grown on alkanes. The tesB-like gene encodes a distinct novel enzyme activity, which acts exclusively on hydroxylated acyl-CoAs and thus represents a hydroxyacyl-CoA-specific thioesterase. Inactivation of this enzyme results in the rechanneling of CoA-activated hydroxylated fatty acids, the cellular intermediates of alkane degradation, towards PHA production. These findings may open up new avenues for the development of simplified biotechnological processes for the production of PHA as a raw material for the production of bioplastics.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sabirova JS,Ferrer M,Lünsdorf H,Wray V,Kalscheuer R,Steinbüchel A,Timmis KN,Golyshin PN

doi

10.1128/JB.01321-06

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

8452-9

issue

24

eissn

0021-9193

issn

1098-5530

pii

JB.01321-06

journal_volume

188

pub_type

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