Abstract:
:RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differential expression of an operon-lacZ fusion bank in rpoS mutant and wild-type backgrounds. Forty-eight independent gene fusions were identified, including several in well-characterized RpoS-regulated genes, such as osmY, katE, and otsA. Many of the other fusions mapped to genes of unknown function or to genes that were not previously known to be under RpoS control. Based on the homology to other known bacterial genes, some of the RpoS-regulated genes of unknown functions are likely important in nutrient scavenging.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Vijayakumar SR,Kirchhof MG,Patten CL,Schellhorn HEdoi
10.1128/JB.186.24.8499-8507.2004keywords:
subject
Has Abstractpub_date
2004-12-01 00:00:00pages
8499-507issue
24eissn
0021-9193issn
1098-5530pii
186/24/8499journal_volume
186pub_type
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