Inducible synthesis of bacterial luciferase: specificity and kinetics of induction.

abstract：:The activation properties of the form I and form II ribulose 1,5-bisphosphate carboxylases from Rhodopseudomonas sphaeroides were examined. Both enzymes have a requirement of Mg2+ for optimal activity. Mn2+, Ni2+, and Co2+ can also support activity of the form I enzyme, whereas only Mn2+ can substitute for Mg2+ with t...

journal_title：Journal of bacteriology

authors： Gibson JL,Tabita FR

更新日期：1979-12-01 00:00:00

abstract：:A periplasmic protein essential for the function of the oligopeptide transport system of Salmonella typhimurium was identified. This protein, encoded by the oppA gene, is one of the most abundant proteins in the periplasm and, with an apparent molecular weight of 52,000, is considerably larger than any other known per...

journal_title：Journal of bacteriology

authors： Higgins CF,Hardie MM

更新日期：1983-09-01 00:00:00

abstract：:During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores i...

journal_title：Journal of bacteriology

authors： Wesp A,Prinz S,Fink GR

更新日期：2001-04-01 00:00:00

abstract：:Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...

journal_title：Journal of bacteriology

authors： Friedberg D,Platko JV,Tyler B,Calvo JM

更新日期：1995-03-01 00:00:00

abstract：:A survey of chromosomal variation in the ST239 clonal group of methicillin-resistant Staphylococcus aureus (MRSA) revealed a novel genetic element, ICE6013. The element is 13,354 bp in length, excluding a 6,551-bp Tn552 insertion. ICE6013 is flanked by 3-bp direct repeats and is demarcated by 8-bp imperfect inverted r...

journal_title：Journal of bacteriology

authors： Smyth DS,Robinson DA

更新日期：2009-10-01 00:00:00

abstract：:At various stages during spore formation sporangia were shocked by cold treatment or with toluene, and the germination requirements of the prespores were examined. Up to 5 h after induction of sporulation (t5) germination was spontaneous; i.e., it occurred without any added germinants. After t5, during stages V and VI...

journal_title：Journal of bacteriology

authors： Dion P,Mandelstam J

更新日期：1980-02-01 00:00:00

abstract：:In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defect...

journal_title：Journal of bacteriology

authors： Matsuyama T,Bhasin A,Harshey RM

更新日期：1995-02-01 00:00:00

abstract：:Arginine utilization in Pseudomonas aeruginosa with multiple catabolic pathways represents one of the best examples of the metabolic versatility of this organism. To identify genes involved in arginine catabolism, we have employed DNA microarrays to analyze the transcriptional profiles of this organism in response to ...

journal_title：Journal of bacteriology

authors： Yang Z,Lu CD

更新日期：2007-06-01 00:00:00

abstract：:The hutC gene in Pseudomonas putida encodes a repressor protein that negatively regulates the expression of all hut genes. We have overexpressed this cloned hutC gene in Escherichia coli to identify P. putida hut regions that could specifically bind the repressor. Ten restriction fragments, some of which were partiall...

journal_title：Journal of bacteriology

authors： Hu L,Allison SL,Phillips AT

更新日期：1989-08-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAggEC) has been associated with persistent pediatric diarrhea in the developing world, yet the pathogenetic mechanisms of EAggEC infection are unknown. Our previous data have suggested that aggregative adherence of some EAggEC strains to HEp-2 cells is mediated by flexible, bundle-...

journal_title：Journal of bacteriology

authors： Nataro JP,Yikang D,Yingkang D,Walker K

更新日期：1994-08-01 00:00:00

abstract：:Shigella spp. are the major cause of bacillary dysentery worldwide. The pathogenic process involves bacterial invasion and lysis of the phagocytic vacuole, followed by replication and movement within the cell cytoplasm and, ultimately, spread directly into adjacent cells. This study demonstrates that S. flexneri cytoc...

journal_title：Journal of bacteriology

authors： Way SS,Sallustio S,Magliozzo RS,Goldberg MB

更新日期：1999-02-01 00:00:00

abstract：:The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicat...

journal_title：Journal of bacteriology

authors： Lafontaine ER,Cope LD,Aebi C,Latimer JL,McCracken GH Jr,Hansen EJ

更新日期：2000-03-01 00:00:00

abstract：:Studies by R. Lin et al. (J. Bacteriol. 174:1948-1955, 1992) suggested that the Escherichia coli leu operon might be a member of the Lrp regulon. Their results were obtained with a leucine auxotroph; in leucine prototrophs grown in a medium lacking leucine, there was little difference in leu operon expression between ...

journal_title：Journal of bacteriology

authors： Landgraf JR,Boxer JA,Calvo JM

更新日期：1999-10-01 00:00:00

abstract：:Tetracycline resistance of three Bacteroides fragilis strains was shown to be inducible by subinhibitory concentrations of tetracycline. Tetracycline resistance markers could be transferred to another B. fragilis strain by filter mating. The transferability was inducible by subinhibitory concentrations of tetracycline...

journal_title：Journal of bacteriology

authors： Rashtchian A,Dubes GR,Booth SJ

更新日期：1982-04-01 00:00:00

abstract：:A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication...

journal_title：Journal of bacteriology

authors： Witkin EM,Roegner-Maniscalco V

更新日期：1992-06-01 00:00:00

abstract：:Escherichia coli K-12 can utilize a gamma-glutamyl peptide as an amino acid source, for which gamma-glutamyltranspeptidase (EC 2.3.2.2) is essential. We suggest that the gamma-glutamyl linkage of a gamma-glutamyl peptide is hydrolyzed by gamma-glutamyltranspeptidase located in the periplasmic space, and the released a...

journal_title：Journal of bacteriology

authors： Suzuki H,Hashimoto W,Kumagai H

更新日期：1993-09-01 00:00:00

abstract：:The region of plasmid NR1 concerned with resistance to Hg2+ and organomercurials consists of sequences found on restriction endonuclease fragments EcoRI-H and EcoRI-I. When both fragments were cloned together into a derivative of plasmid ColE1, the hybrid plasmid conferred properties indistinguishable from those of th...

journal_title：Journal of bacteriology

authors： Nakahara H,Silver S,Miki T,Rownd RH

更新日期：1979-10-01 00:00:00

abstract：:The cheW gene from Escherichia coli has been cloned an inducible promoter, and the effects of the overproduction of the CheW protein on chemotactic behavior and receptor covalent modification have been examined. Plasmids that contain the cheW gene behind a regulatable promoter complement a cheW mutation when the CheW ...

journal_title：Journal of bacteriology

authors： Sanders DA,Mendez B,Koshland DE Jr

更新日期：1989-11-01 00:00:00

abstract：:High-affinity iron acquisition in Bacillus subtilis is mediated via the bacillibactin catechole siderophore pathway. Three of the four essential pathway steps, bacillibactin synthesis, Fe-bacillibactin uptake, and Fe-bacillibactin hydrolysis have been characterized previously. The functional and regulatory components ...

journal_title：Journal of bacteriology

authors： Miethke M,Schmidt S,Marahiel MA

更新日期：2008-08-01 00:00:00

abstract：:Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...

journal_title：Journal of bacteriology

authors： Cybulska J,Jeljaszewicz J

更新日期：1966-03-01 00:00:00

abstract：:Studies with methionineless strains of Escherichia coli WWU and Bacillus megaterium KM have shown that selenomethionine only partially satisfies their methionine requirement. ...

journal_title：Journal of bacteriology

authors： Wu M,Wachsman JT

更新日期：1970-12-01 00:00:00

abstract：:High-pressure liquid chromatography of a muramidase digest of murein sacculi from Caulobacter crescentus showed that the absence of D-alanine carboxypeptidase activity in the cells was reflected by a very high content of pentapeptide in the murein. Approximately half of the pentapeptide side chains were shown to conta...

journal_title：Journal of bacteriology

authors： Markiewicz Z,Glauner B,Schwarz U

更新日期：1983-11-01 00:00:00

abstract：:Cultures of recombination-deficient strains of Escherichia coli are composed of three classes of cells: (i) viable cells, which can undergo 20 or more generations, (ii) residually dividing cells, which can undergo fewer than 20 generations (probably an average of fewer than 6), and (iii) nondividing cells, which are i...

journal_title：Journal of bacteriology

authors： Capaldo FN,Ramsey G,Barbour SD

更新日期：1974-04-01 00:00:00

abstract：:A Pseudomonas strain produced an enzyme capable of deacylating 7 beta-(4-carboxybutanamido)cephalosporanic acid to 7-aminocephalosporanic acid in response to glutaric acid. The gene for the enzyme was cloned within the PstI site of pBR325 as a 7.35-kilobase-pair DNA segment from a mutant of this strain whose enzyme is...

journal_title：Journal of bacteriology

authors： Matsuda A,Komatsu KI

更新日期：1985-09-01 00:00:00

abstract：:A chromosomal insertion of transposon Tn917 partially restores the expression of protease and alpha-toxin activities to PM466, a genetically defined agr-null derivative of the wild-type Staphylococcus aureus strain RN6390. In co-transduction experiments, transposon-encoded erythromycin resistance and a protease- and a...

journal_title：Journal of bacteriology

authors： McNamara PJ,Milligan-Monroe KC,Khalili S,Proctor RA

更新日期：2000-06-01 00:00:00

abstract：:Derivatives of Pseudomonas sp. B13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the TOL plasmid of Pseudomonas putida mt-2 were studied. The utilization of these substrates, a property not shared by the parent strains, was shown to dep...

journal_title：Journal of bacteriology

authors： Reineke W,Knackmuss HJ

更新日期：1980-05-01 00:00:00

abstract：:Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, grown on methanol was found to show dihydroxyacetone synthase, dihydroxyacetone kinase, and ribulose 1,5-bisphosphate carboxylase, but no hydroxypyruvate reductase and very low hexulose 6-phosphate synthase, activities. The dihydroxyacetone synthase was foun...

journal_title：Journal of bacteriology

authors： Ro YT,Eom CY,Song T,Cho JW,Kim YM

更新日期：1997-10-01 00:00:00

abstract：:The copy number and stability of artificial 2-micron circle-based plasmids have been accurately measured in [Cir+] and [Cir0] strains of Saccharomyces cerevisiae. We conclude that (i) instability and copy number vary greatly from plasmid to plasmid; (ii) instability and copy number are negatively correlated--that is, ...

journal_title：Journal of bacteriology

authors： Futcher AB,Cox BS

更新日期：1984-01-01 00:00:00

abstract：:Tetracycline accumulation by the phototrophic bacterium Rhodopseudomonas sphaeroides has been studied, using the fluorescence properties of the antibiotic and measuring uptake of [7- 3H]tetracycline. Accumulation was carrier mediated, with a Km of approximately 300 micronM. Efflux also appeared to be carried mediated,...

journal_title：Journal of bacteriology

authors： Weckesser J,Magnuson JA

更新日期：1979-06-01 00:00:00

abstract：:Hertman, I. (Israel Institute for Biological Research, Ness Ziona, Israel). Bacteriophage common to Pasteurella pestis and Escherichia coli. J. Bacteriol. 88:1002-1005. 1964.-Phage Y of Pasteurella pestis and phage T(3) of Escherichia coli are serologically related and have common bacterial hosts. Anti-Y serum neutral...

journal_title：Journal of bacteriology

authors： HERTMAN I

更新日期：1964-10-01 00:00:00