Identification, cloning, and initial characterization of rot, a locus encoding a regulator of virulence factor expression in Staphylococcus aureus.

abstract：:Previously we have described a mutant Hfr strain in which incompatibility between the integrated F factor and an autonomous F-prime (F') factor was abolished. The mutation (inc) was located in the integrated F factor. F-prime factors isolated from the mutant Hfr strain have the same incompatibility behavior as those i...

journal_title：Journal of bacteriology

authors： Pfister A,DeVries JK,Maas WK

更新日期：1976-07-01 00:00:00

abstract：:The dnaA operon of Escherichia coli contains the genes dnaA, dnaN, and recF encoding DnaA, beta clamp of DNA polymerase III holoenzyme, and RecF. When the DnaA concentration is raised, an increase in the number of DNA replication initiation events but a reduction in replication fork velocity occurs. Because DnaA is au...

journal_title：Journal of bacteriology

authors： Grigorian AV,Lustig RB,Guzmán EC,Mahaffy JM,Zyskind JW

更新日期：2003-01-01 00:00:00

abstract：:Several resistant mutants of a transformable group H streptococcus, strain Challis, were isolated from media containing high concentrations of streptomycin. Mutants SR5a and SR5 exhibited high and low transformability, respectively, when exposed to deoxyribonucleic acid (DNA) from a novobiocin-resistant Challis strain...

journal_title：Journal of bacteriology

authors： Perry D

更新日期：1968-01-01 00:00:00

abstract：:PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the v...

journal_title：Journal of bacteriology

authors： Trott AE,Stevens AM

更新日期：2001-01-01 00:00:00

abstract：:The heat shock response of Bradyrhizobium japonicum is controlled by a complex network involving two known regulatory systems. While some heat shock genes are controlled by a highly conserved inverted-repeat structure (CIRCE), others depend on a sigma 32-type heat shock sigma factor. Using Western blot (immunoblot) an...

journal_title：Journal of bacteriology

authors： Narberhaus F,Weiglhofer W,Fischer HM,Hennecke H

更新日期：1996-09-01 00:00:00

abstract：:Pentalenolactone (PL) irreversibly inactivates the enzyme glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating)] (EC 1.2.1.12) and thus is a potent inhibitor of glycolysis in both procaryotic and eucaryotic cells. We showed that PL-producing strain Streptomyces ar...

journal_title：Journal of bacteriology

authors： Maurer KH,Pfeiffer F,Zehender H,Mecke D

更新日期：1983-02-01 00:00:00

abstract：:The tetracycline resistance element of plasmid pBR322 partially complements the potassium transport defect of Escherichia coli K-12 mutants having markedly impaired K+ transport. The plasmid increases K+ transport. The Tn10 element does not result in increased transport, demonstrating that the effect is not general fo...

journal_title：Journal of bacteriology

authors： Dosch DC,Salvacion FF,Epstein W

更新日期：1984-12-01 00:00:00

abstract：:After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a ...

journal_title：Journal of bacteriology

authors： Sargent MG,Bennett MF

更新日期：1986-04-01 00:00:00

abstract：:We have cloned a DNA fragment from a genomic library of Myxococcus xanthus using an oligonucleotide probe representing conserved regions of biotin carboxylase subunits of acetyl coenzyme A (acetyl-CoA) carboxylases. The fragment contained two open reading frames (ORF1 and ORF2), designated the accB and accA genes, cap...

journal_title：Journal of bacteriology

authors： Kimura Y,Miyake R,Tokumasu Y,Sato M

更新日期：2000-10-01 00:00:00

abstract：:Tetracycline accumulation by the phototrophic bacterium Rhodopseudomonas sphaeroides has been studied, using the fluorescence properties of the antibiotic and measuring uptake of [7- 3H]tetracycline. Accumulation was carrier mediated, with a Km of approximately 300 micronM. Efflux also appeared to be carried mediated,...

journal_title：Journal of bacteriology

authors： Weckesser J,Magnuson JA

更新日期：1979-06-01 00:00:00

abstract：:Pseudomonas cepacia mutants deficient in either 6-phosphogluconate (6PGA) dehydratase (Edd-) or 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase (Eda-) failed to utilize glucose or gluconate despite the prominence of of 6-phosphogluconate dehydrogenase (6PGAD) ii this bacterium and the potential for utilizing the pen...

journal_title：Journal of bacteriology

authors： Allenza P,Lessie TG

更新日期：1982-06-01 00:00:00

abstract：:The gene (hmgA) for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) from the thermophilic archaeon Sulfolobus solfataricus P2 was cloned and sequenced. S. solfataricus HMG-CoA reductase exhibited a high degree of sequence identity (47%) to the HMG-CoA reductase of the halophilic archaeon Halofe...

journal_title：Journal of bacteriology

authors： Bochar DA,Brown JR,Doolittle WF,Klenk HP,Lam W,Schenk ME,Stauffacher CV,Rodwell VW

更新日期：1997-06-01 00:00:00

abstract：:A coordinated response to a complex and dynamic environment requires an organism to simultaneously monitor and interpret multiple signaling cues. In bacteria and some eukaryotes, environmental responses depend on the histidine autokinases (HKs). For example, VirA, a large integral membrane HK from Agrobacterium tumefa...

journal_title：Journal of bacteriology

authors： Gao R,Lynn DG

更新日期：2007-08-01 00:00:00

abstract：:Cyclic di-AMP (c-di-AMP) is an essential and ubiquitous second messenger among bacteria. c-di-AMP regulates many cellular pathways through direct binding to several molecular targets in bacterial cells. c-di-AMP depletion is well known to destabilize the bacterial cell wall, resulting in increased bacteriolysis and en...

journal_title：Journal of bacteriology

authors： Massa SM,Sharma AD,Siletti C,Tu Z,Godfrey JJ,Gutheil WG,Huynh TN

更新日期：2020-11-19 00:00:00

abstract：:Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, the uptake of radioactive adenine and lysine was extremely lim...

journal_title：Journal of bacteriology

authors： McCusker JH,Haber JE

更新日期：1977-10-01 00:00:00

abstract：:The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that int...

journal_title：Journal of bacteriology

authors： Ishigo-Oka D,Ogasawara N,Moriya S

更新日期：2001-03-01 00:00:00

abstract：:Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be es...

journal_title：Journal of bacteriology

authors： Pi J,Wookey PJ,Pittard AJ

更新日期：1993-11-01 00:00:00

abstract：:Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intrace...

journal_title：Journal of bacteriology

authors： Johnson DL,Stone CB,Mahony JB

更新日期：2008-04-01 00:00:00

abstract：:31P and 13C nuclear magnetic resonance (NMR) experiments were performed on suspensions of the phototrophic bacterium Chromatium vinosum incubated anaerobically in the dark. 31P NMR spectra revealed that during prolonged dark incubation high ATP levels are maintained. This phenomenon was independent of the presence of ...

journal_title：Journal of bacteriology

authors： Nicolay K,van Gemerden H,Hellingwerf KJ,Konings WN,Kaptein R

更新日期：1983-08-01 00:00:00

abstract：:Bacteriocin JF246 has been purified from mitomycin C-induced Serratia marcescens cells by salt extraction, ammonium sulfate fractionation, and chromatography on QAE-Sephadex and SP-Sephadex. The purified material is homogeneous on polyacrylamide gel electrophoresis in the presence of 2% sodium dodecyl sulfate or 6 m u...

journal_title：Journal of bacteriology

authors： Foulds J

更新日期：1972-06-01 00:00:00

abstract：:Teichoic acid-like material extracted by cold trichloroacetic acid from lyophilized whole cells of streptococci from groups A,D,E,O, and T was shown to give a positive precipitin reaction with group antisera. Similar material from cells of groups B,C,F,G,H,K,L,M,N,P,Q,R, and S did not give a positive reaction with gro...

journal_title：Journal of bacteriology

authors： Matsuno T,Slade HD

更新日期：1970-06-01 00:00:00

abstract：:Caulobacter is a distinctive genus of prosthecate bacteria. Because caulobacters adhere to surfaces and are found in diverse locales, their role in oligotrophic environments and bacterial biofilm communities is of interest. The phylogenetic relationships of a group of marine and freshwater caulobacters were examined i...

journal_title：Journal of bacteriology

authors： Stahl DA,Key R,Flesher B,Smit J

更新日期：1992-04-01 00:00:00

abstract：:We screened Salmonella typhimurium, Citrobacter freundii, Klebsiella pneumoniae, Shigella boydii, and many isolates of Escherichia coli for DNA sequences homologous to those encoding each of two unrelated type I restriction and modification systems (EcoK and EcoA). Both K- and A-related hsd genes were identified, but ...

journal_title：Journal of bacteriology

authors： Daniel AS,Fuller-Pace FV,Legge DM,Murray NE

更新日期：1988-04-01 00:00:00

abstract：:Extraction with pentane virtually abolished reduced nicotinamide adenine dinucleotide oxidase activity in small particles from Azotobacter vinelandii, but activity was largely restored by added ubiquinone. ...

journal_title：Journal of bacteriology

authors： Swank RT,Burris RH

更新日期：1969-04-01 00:00:00

abstract：:The Bacillus subtilis ferric uptake regulator (Fur) protein mediates the iron-dependent repression of at least 20 operons encoding approximately 40 genes. We investigated the physiological roles of Fur-regulated genes by the construction of null mutations in 14 transcription units known or predicted to function in sid...

journal_title：Journal of bacteriology

authors： Ollinger J,Song KB,Antelmann H,Hecker M,Helmann JD

更新日期：2006-05-01 00:00:00

abstract：:The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The observed binding forces between fibrinogen or fibronectin and S. aureus MSCRAMMs occurred as an approximate integer multiple o...

journal_title：Journal of bacteriology

authors： Simpson KH,Bowden G,Höök M,Anvari B

更新日期：2003-03-01 00:00:00

abstract：:The expression of the positively charged human protein secretory leukocyte protease inhibitor (SLPI) in Escherichia coli causes severe cellular toxicity. After induction of SLPI synthesis in a high-level-expression strain, SGE61, the growth of the strain is arrested and total protein and RNA synthesis rates decline by...

journal_title：Journal of bacteriology

authors： Miller KW,Evans RJ,Eisenberg SP,Thompson RC

更新日期：1989-04-01 00:00:00

abstract：:In all organisms, chromosome replication is regulated mainly at the initiation step. Most of the knowledge about the mechanisms that regulate replication initiation in bacteria has come from studies on rod-shaped bacteria, such as Escherichia coli and Bacillus subtilisStreptomyces is a bacterial genus that is characte...

journal_title：Journal of bacteriology

authors： Łebkowski T,Wolański M,Ołdziej S,Flärdh K,Zakrzewska-Czerwińska J

更新日期：2020-01-15 00:00:00

abstract：:The effects of bdellovibrio infection on host permeability and respiration were investigated by measuring respiration rates and the rate of o-nitrophenyl-beta-d-galactopyranoside hydrolysis during the course of single infection cycles of Bdellovibrio bacteriovorus strain 109 growing on Escherichia coli ML 35 (lac i(-)...

journal_title：Journal of bacteriology

authors： Rittenberg SC,Shilo M

更新日期：1970-04-01 00:00:00

abstract：:We are engaged in the genetic and physical characterization of an enterotoxin (Ent) plasmid, Ent P307, which contains genes for the production of a hear-labile and a heat-stable enterotoxin. We are using an Escherichia coli K-12 strain, 711 (P307), constructed by S. Falkow, which contains no other plasmids besides Ent...

journal_title：Journal of bacteriology

authors： Santos DS,Palchaudhuri S,Maas WK

更新日期：1975-12-01 00:00:00