In vivo 31P and 13C nuclear magnetic resonance studies of acetate metabolism in Chromatium vinosum.

Abstract:

:31P and 13C nuclear magnetic resonance (NMR) experiments were performed on suspensions of the phototrophic bacterium Chromatium vinosum incubated anaerobically in the dark. 31P NMR spectra revealed that during prolonged dark incubation high ATP levels are maintained. This phenomenon was independent of the presence of the energy reserves polyglucose and polyphosphate. 13C NMR experiments revealed that the amino acids glutamate, aspartate, and alanine are the major products of acetate incorporation in the dark. Apart from these amino acids, poly-beta-hydroxybutyrate was also formed. Acetate metabolism was markedly stimulated by the presence of polyglucose. The specific 13C activity of glutamate C-2 was approximately 50% that of glutamate C-4. The idea is discussed that this difference is the consequence of the maintenance of redox balance during entry of acetate into cell metabolism.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Nicolay K,van Gemerden H,Hellingwerf KJ,Konings WN,Kaptein R

doi

10.1128/JB.155.2.634-642.1983

subject

Has Abstract

pub_date

1983-08-01 00:00:00

pages

634-42

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

155

pub_type

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