Cloning, sequence analysis, and functional expression of the acetyl coenzyme A synthetase gene from Methanothrix soehngenii in Escherichia coli.

Abstract:

:In the acetoclastic methanogen Methanothrix soehngenii, acetate is activated to acetyl coenzyme A by acetyl coenzyme A synthetase (Acs). The acs gene, coding for the single Acs subunit, was isolated from a genomic library of M. soehngenii DNA in Escherichia coli by using antiserum raised against the purified Acs. After introduction in E. coli, the acs gene was expressed, resulting in the production of an immunoreactive protein of 68 kDa, which is approximately 5 kDa smaller than the known size of purified Acs. In spite of this difference in size, the Acs enzymes are produced in similar quantities in E. coli and M. soehngenii and show comparable specific activities. Upstream from the acs gene, consensus archaeal expression signals were identified. Immediately downstream from the acs gene there was a putative transcriptional stop signal. The amino acid sequence deduced from the nucleotide sequence of the acs gene showed homology with those of functionally related proteins, i.e., proteins involved in the binding of coenzyme A, ATP, or both.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Eggen RI,Geerling AC,Boshoven AB,de Vos WM

doi

10.1128/jb.173.20.6383-6389.1991

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

6383-9

issue

20

eissn

0021-9193

issn

1098-5530

journal_volume

173

pub_type

杂志文章
  • Recombination in Escherichia coli and the definition of biological species.

    abstract::The DNA sequence of part of the gnd (6-phosphogluconate dehydrogenase) gene was determined for eight wild strains of Escherichia coli and for Salmonella typhimurium. Since a region of the trp (tryptophan) operon and the phoA (alkaline phosphatase) gene have been sequenced from the same strains, the gene trees for thes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.22.7257-7268.1991

    authors: Dykhuizen DE,Green L

    更新日期:1991-11-01 00:00:00

  • Functional organization of the autotransporter adhesin involved in diffuse adherence.

    abstract::The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is a multifunctional autotransporter protein that mediates bacterial aggregation and biofilm formation, as well as adhesion and invasion of cultured epithelial cells. To elucidate the structure-function relationships of AIDA-I, we performed transposon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01238-07

    authors: Charbonneau ME,Mourez M

    更新日期:2007-12-01 00:00:00

  • Conservation and variation of nucleotide sequences within related bacterial genomes: Escherichia coli strains.

    abstract::Changes in the patterns produced by annealing restriction endonuclease digests of bacterial genomes with probe deoxyribonucleic acids (DNAs) containing small portions of a bacterial genome provide sensitive indicator of the degree of nucleotide sequence relatedness that exists in localized regions of the genomes of cl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.1.355-365.1980

    authors: Anilionis A,Riley M

    更新日期:1980-07-01 00:00:00

  • Glycerol metabolism and PrfA activity in Listeria monocytogenes.

    abstract::Listeria monocytogenes is able to efficiently utilize glycerol as a carbon source. In a defined minimal medium, the growth rate (during balanced growth) in the presence of glycerol is similar to that in the presence of glucose or cellobiose. Comparative transcriptome analyses of L. monocytogenes showed high-level tran...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00259-08

    authors: Joseph B,Mertins S,Stoll R,Schär J,Umesha KR,Luo Q,Müller-Altrock S,Goebel W

    更新日期:2008-08-01 00:00:00

  • Collaborative regulation of Escherichia coli glutamate-dependent acid resistance by two AraC-like regulators, GadX and GadW (YhiW).

    abstract::An important feature of Escherichia coli pathogenesis is an ability to withstand extremely acidic environments of pH 2 or lower. This acid resistance property contributes to the low infectious dose of pathogenic E. coli species. One very efficient E. coli acid resistance system encompasses two isoforms of glutamate de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.24.7001-7012.2002

    authors: Ma Z,Richard H,Tucker DL,Conway T,Foster JW

    更新日期:2002-12-01 00:00:00

  • BIOCHEMICAL STUDIES OF THE SMOOTH-ROUGH MUTATION IN SALMONELLA MINNESOTA.

    abstract::Lüderitz, O. (Max-Planck-Institut für Immunbiologie, Frieburg, Germany), H. J. Risse, H. Schulte-Holthausen, J. L. Strominger, I. W. Sutherland, and O. Westphal. Biochemical studies of the smooth-rough mutation in Salmonella minnesota. J. Bacteriol. 89:343-354. 1965.-A comparative study of the O antigen from the smoot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.2.343-354.1965

    authors: LUEDERITZ O,RISSE HJ,SCHULTE-HOLTHAUSEN H,STROMINGER JL,SUTHERLAND IW,WESTPHAL O

    更新日期:1965-02-01 00:00:00

  • Biofilm formation and dispersal under the influence of the global regulator CsrA of Escherichia coli.

    abstract::The predominant mode of growth of bacteria in the environment is within sessile, matrix-enclosed communities known as biofilms. Biofilms often complicate chronic and difficult-to-treat infections by protecting bacteria from the immune system, decreasing antibiotic efficacy, and dispersing planktonic cells to distant b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.290-301.2002

    authors: Jackson DW,Suzuki K,Oakford L,Simecka JW,Hart ME,Romeo T

    更新日期:2002-01-01 00:00:00

  • Intracellular concentrations of 65 species of transcription factors with known regulatory functions in Escherichia coli.

    abstract::The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01579-14

    authors: Ishihama A,Kori A,Koshio E,Yamada K,Maeda H,Shimada T,Makinoshima H,Iwata A,Fujita N

    更新日期:2014-08-01 00:00:00

  • Molecular cloning of the uhp region and evidence for a positive activator for expression of the hexose phosphate transport system of Escherichia coli.

    abstract::The uhp locus of Escherichia coli contains genes for the sugar phosphate transport system (uhpT) and the regulatory system which allows its induction by external glucose 6-phosphate (uhpRA). The uhp region was cloned onto high-copy-number plasmids, both from Uhp(+) plasmids of the Clarke-Carbon collection and from gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1062-1070.1983

    authors: Shattuck-Eidens DM,Kadner RJ

    更新日期:1983-09-01 00:00:00

  • Regulation and function of cytochrome c' in Rhodobacter sphaeroides 2.4.3.

    abstract::Cytochrome c' (Cyt c') is a c-type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in Rhodobacter sphaeroides 2.4.3, designated cycP, was isolated and sequenced. Northern blot analysis and beta-galactosidase assays demonstrated that cycP transcription increased as oxygen levels decreased an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.12.4077-4085.2005

    authors: Choi PS,Grigoryants VM,Abruña HD,Scholes CP,Shapleigh JP

    更新日期:2005-06-01 00:00:00

  • Genome sequence of Mycoplasma iowae strain 695, an unusual pathogen causing deaths in turkeys.

    abstract::Mycoplasma iowae is associated mainly with reduced hatchability in turkeys and is well known for the unusual ability of phenotypic variation in the Mycoplasma surface components as well as a relative resistance to heat, bile salts, and many antimicrobials. A subset of unique genes and a gene cluster responsible for th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06297-11

    authors: Wei S,Guo Z,Li T,Zhang T,Li X,Zhou Z,Li Z,Liu M,Luo R,Bi D,Chen H,Zhou R,Jin H

    更新日期:2012-01-01 00:00:00

  • Bap, a Staphylococcus aureus surface protein involved in biofilm formation.

    abstract::Identification of new genes involved in biofilm formation is needed to understand the molecular basis of strain variation and the pathogenic mechanisms implicated in chronic staphylococcal infections. A biofilm-producing Staphylococcus aureus isolate was used to generate biofilm-negative transposon (Tn917) insertion m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.9.2888-2896.2001

    authors: Cucarella C,Solano C,Valle J,Amorena B,Lasa I,Penadés JR

    更新日期:2001-05-01 00:00:00

  • Repair of radiation-induced damage in Escherichia coli. II. Effect of rec and uvr mutations on radiosensitivity, and repair of x-ray-induced single-strand breaks in deoxyribonucleic acid.

    abstract::Strains of Escherichia coli K-12 mutant in the genes controlling excision repair (uvr) and genetic recombination (rec) have been studied with reference to their radiosensitivity and their ability to repair X-ray-induced single-strand breaks in deoxyribonucleic acid (DNA). Mutations in the rec genes appreciably increas...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.103.1.49-54.1970

    authors: Kapp DS,Smith KC

    更新日期:1970-07-01 00:00:00

  • Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.

    abstract::To elucidate the biological functions of the ribosomal protein L34, which is encoded by the rpmH gene, the rpmH deletion mutant of Bacillus subtilis and two suppressor mutants were characterized. Although the ΔrpmH mutant exhibited a severe slow-growth phenotype, additional mutations in the yhdP or mgtE gene restored ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01896-14

    authors: Akanuma G,Kobayashi A,Suzuki S,Kawamura F,Shiwa Y,Watanabe S,Yoshikawa H,Hanai R,Ishizuka M

    更新日期:2014-11-01 00:00:00

  • NEW TYPE OF STREPTOMYCIN RESISTANCE RESULTING FROM ACTION OF THE EPISOMELIKE MUTATOR FACTOR IN ESCHERICHIA COLI.

    abstract::Gundersen, Wenche B. (Oslo University, Oslo, Norway). New type of streptomycin resistance resulting from action of the episomelike mutator factor in Escherichia coli. J. Bacteriol. 86:510-516. 1963.-Analyses have been performed to elucidate the genetic nature of the streptomycin resistance that results from the action...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.3.510-516.1963

    authors: GUNDERSEN WB

    更新日期:1963-09-01 00:00:00

  • Genetic, molecular, and functional analysis of Streptococcus faecalis R plasmid pJH1.

    abstract::Streptococcus faecalis JH1 contains two conjugative plasmids, pJH1, an R plasmid that codes for resistance to kanamycin, streptomycin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Strain JH1 was used as an antibiotic resistance donor in conjugation experiments with two plasmid-free S. fae...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1094-1104.1983

    authors: Banai M,LeBlanc DJ

    更新日期:1983-09-01 00:00:00

  • Expression of Adhesive Pili and the Collagen-Binding Adhesin Ace Is Activated by ArgR Family Transcription Factors in Enterococcus faecalis.

    abstract::It was shown previously that the disruption of the ahrC gene encoding a predicted ArgR family transcription factor results in a severe defect in biofilm formation in vitro, as well as a significant attenuation of virulence of Enterococcus faecalis strain OG1RF in multiple experimental infection models. Using transcrip...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00269-18

    authors: Manias DA,Dunny GM

    更新日期:2018-08-24 00:00:00

  • Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824.

    abstract::A gene (aad) coding for an aldehyde/alcohol dehydrogenase (AAD) was identified immediately upstream of the previously cloned ctfA (J. W. Cary, D. J. Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.3.871-885.1994

    authors: Nair RV,Bennett GN,Papoutsakis ET

    更新日期:1994-02-01 00:00:00

  • The requirement for pneumococcal MreC and MreD is relieved by inactivation of the gene encoding PBP1a.

    abstract::MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05245-11

    authors: Land AD,Winkler ME

    更新日期:2011-08-01 00:00:00

  • Novel class of mutations of pilS mutants, encoding plasmid R64 type IV prepilin: interface of PilS-PilV interactions.

    abstract::The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01204-07

    authors: Shimoda E,Muto T,Horiuchi T,Furuya N,Komano T

    更新日期:2008-02-01 00:00:00

  • A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.

    abstract::In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.12.3864-3871.1991

    authors: Vartak NB,Liu L,Wang BM,Berg CM

    更新日期:1991-06-01 00:00:00

  • Translational autoregulation of ermC 23S rRNA methyltransferase expression in Bacillus subtilis.

    abstract::ermC specifies an rRNA methyltransferase that confers resistance to erythromycin. The expression of this determinant is induced by the addition of erythromycin. The induction mechanism has been shown to operate posttranscriptionally, and its mechanism has been elucidated. We now show that synthesis of the ermC gene pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.3.1133-1141.1986

    authors: Denoya CD,Bechhofer DH,Dubnau D

    更新日期:1986-12-01 00:00:00

  • Bacteriocin protein BacL1 of Enterococcus faecalis targets cell division loci and specifically recognizes L-Ala2-cross-bridged peptidoglycan.

    abstract::Bacteriocin 41 (Bac41) is produced from clinical isolates of Enterococcus faecalis and consists of two extracellular proteins, BacL1 and BacA. We previously reported that BacL1 protein (595 amino acids, 64.5 kDa) is a bacteriolytic peptidoglycan D-isoglutamyl-L-lysine endopeptidase that induces cell lysis of E. faecal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02203-14

    authors: Kurushima J,Nakane D,Nishizaka T,Tomita H

    更新日期:2015-01-01 00:00:00

  • Pneumolysin localizes to the cell wall of Streptococcus pneumoniae.

    abstract::Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01489-08

    authors: Price KE,Camilli A

    更新日期:2009-04-01 00:00:00

  • Oligomerization of the bacteriophage lambda S protein in the inner membrane of Escherichia coli.

    abstract::Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inne...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.912-921.1990

    authors: Zagotta MT,Wilson DB

    更新日期:1990-02-01 00:00:00

  • The NtcA-regulated amtB gene is necessary for full methylammonium uptake activity in the cyanobacterium Synechococcus elongatus.

    abstract::The Amt proteins constitute a ubiquitous family of transmembrane ammonia channels that permit the net uptake of ammonium by cells. In many organisms, there is more than one amt gene, and these genes are subjected to nitrogen control. The mature Amt protein is a homo- or heterooligomer of three Amt subunits. We previou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00404-07

    authors: Paz-Yepes J,Herrero A,Flores E

    更新日期:2007-11-01 00:00:00

  • Identification of phthiodiolone ketoreductase, an enzyme required for production of mycobacterial diacyl phthiocerol virulence factors.

    abstract::Diacyl phthiocerol esters and their congeners are mycobacterial virulence factors. The biosynthesis of these complex lipids remains poorly understood. Insight into their biosynthesis will aid the development of rationally designed drugs that inhibit their production. In this study, we investigate a biosynthetic step r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.14.4760-4766.2005

    authors: Onwueme KC,Vos CJ,Zurita J,Soll CE,Quadri LE

    更新日期:2005-07-01 00:00:00

  • Nucleotide sequence analysis and expression of the minimum REPI replication region and incompatibility determinants of pColV-K30.

    abstract::We sequenced the minimum REPI replication region and the incompatibility determinants of pColV-K30. The minimum replication region contains an open reading frame which corresponds to a 35-kilodalton (kDa) protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis with maxicells transformed with a numb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.4.2195-2201.1989

    authors: Perez-Casal JF,Gammie AE,Crosa JH

    更新日期:1989-04-01 00:00:00

  • Bacillus subtilis locus encoding a killer protein and its antidote.

    abstract::We have isolated mutations that block sporulation after formation of the polar septum in Bacillus subtilis. These mutations were mapped to the two genes of a new locus, spoIIS. Inactivation of the second gene, spoIISB, decreases sporulation efficiency by 4 orders of magnitude. Inactivation of the first gene, spoIISA, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.12.3574-3581.2001

    authors: Adler E,Barák I,Stragier P

    更新日期:2001-06-01 00:00:00

  • Possible utility of a fluorescent antibody technique in the serological identification of antagonistic Streptomyces.

    abstract::Arai, Tadashi (Chiba University, Chiba, Japan), Shyuko Kuroda, and Michio Ito. Possible utility of a fluorescent antibody technique in the serological identification of antagonistic Streptomyces. J. Bacteriol. 83:20-26. 1962.-The indirect fluorescent antibody technique was extended to the serological investigation of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.1.20-26.1962

    authors: ARAI T,KURODA S,ITO M

    更新日期:1962-01-01 00:00:00