Abstract:
:Analysis of deoxyribonucleic acid (DNA) from four species of Clostridium, including two thermophiles, a mesophile, and a psychrophile, revealed no obvious relationship between growth temperature and DNA base composition. The melting temperatures (T(m)) of the DNA from the four species varied no more among the thermophilic, mesophilic, and psychrophilic species than among many related mesophilic species. Characterization of ribosomes from the clostridia by means of optical rotatory dispersion yielded similar spectra in common with other unrelated organisms. Only small differences were noted in the base composition of ribosomal ribonucleic acid (RNA) and in the amino acid composition of ribosomal proteins, including half-cystine content, as determined by cysteic acid analysis, and accessible sulfhydryl groups, as determined by titration with dithiobis (2-nitrobenzoic acid). Except for the two thermophiles, the ribosomal protein electrophoretic patterns were dissimilar. No unusual thermal stability was manifested in the T(m) values of thermophile ribosomal RNA. However, thermophile ribosome T(m) values (69 C) were higher than were mesophile and psychrophile T(m) values (64 C). Ribosomes from the four clostridial species were also examined in regard to the effect of heat on their functional integrity, measured by their activity in poly U-directed (14)C-phenylaline incorporation, and their gross physical integrity, measured by sucrose gradient analysis. The T(d, 5) values (temperature which produces 50% inactivation after 5 min) was found to be 70 and 72 C for the two thermophiles C. tartarivorum and C. thermosaccharolyticum, respectively; 57 C for a mesophile, C. pasteurianum; and 53 C for a psychrophile, Clostridium sp. strain 69. At 55 C, little effect was seen on the thermophile ribosomes, but the mesophile ribosomes lost 90% of their activity in 1 hr, and psychrophile ribosomes lost 100% of their activity within 10 min. According to sucrose gradient profiles, heating at 55 C results in dissociation of mesophile ribosomes and aggregation of psychrophile ribosomes. Thermophile S-100 fractions were also more thermostable than were mesophile or psychrophile S-100 fractions. The T(d, 5) values were 69 C for C. tartarivorum and C. thermosaccharolyticum S-100 and 41 C for C. pasteurianum and Clostridium sp. strain 69 S-100. The effect of heat on the endogenous incorporation of (14)C-valine by polysomes was also examined. In the case of thermophile polysomes, the extent of incorporation at 55 and 37 C was about equal. In the case of mesophile and psychrophile polysomes, the extent at 55 C was 44 and 39%, respectively, of the value at 37 C. The initial rates of incorporation in all four cases were greater at 55 C than at 37 C.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Irwin CC,Akagi JM,Himes RHdoi
10.1128/JB.113.1.252-262.1973subject
Has Abstractpub_date
1973-01-01 00:00:00pages
252-62issue
1eissn
0021-9193issn
1098-5530journal_volume
113pub_type
杂志文章abstract::The cold shock response of Escherichia coli is elicited by downshift of temperature from 37 degrees C to 15 degrees C and is characterized by induction of several cold shock proteins, including CsdA, during the acclimation phase. CsdA, a DEAD-box protein, has been proposed to participate in a variety of processes, suc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00655-07
更新日期:2007-08-01 00:00:00
abstract::Supplementation of the growth medium with high concentrations of NaCl, KCl, or sucrose caused a drastic change in the ratio of the two peptidoglycan-associated major outer membrane proteins of Escherichia coli K-12 in that the amounts of proteins b and c present in cell envelope preparations decreased and increased, r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.2.623-630.1977
更新日期:1977-08-01 00:00:00
abstract::Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.23.6615-6624.2002
更新日期:2002-12-01 00:00:00
abstract::Immunity to leptospirosis is principally humorally mediated and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. The only protective antigen identified to date is the leptospiral lipopolysaccharide (LPS), which biochemically resembles typical gram-negative LPS but has greatly reduc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.4.1262-1267.1997
更新日期:1997-02-01 00:00:00
abstract::For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.1165-1167.1984
更新日期:1984-06-01 00:00:00
abstract::Bacteroides ovatus, a human colonic anaerobe, utilizes the galactomannan guar gum as a sole source of carbohydrate. Previously, we found that none of the galactomannan-degrading enzymes were extracellular, and we characterized an outer membrane mannanase which hydrolyzes the backbone of guar gum to produce large fragm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2038-2043.1987
更新日期:1987-05-01 00:00:00
abstract::The 5,10-methylenetetrahydrofolate dehydrogenase of heterotrophically grown Peptostreptococcus productus Marburg was purified to apparent homogeneity. The purified enzyme catalyzed the reversible oxidation of methylenetetrahydrofolate with NADP+ as the electron acceptor at a specific activity of 627 U/mg of protein. T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.4.1414-1419.1991
更新日期:1991-02-01 00:00:00
abstract::Weaver, Judith R. (Iowa State University, Ames), and P. A. Pattee. Inducible resistance to erythromycin in Staphylococcus aureus. J. Bacteriol. 88:574-580. 1964.-The dissociated resistance of Staphylococcus aureus to erythromycin was examined and was found to possess the characteristics of an inducible enzyme. The ind...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.574-580.1964
更新日期:1964-09-01 00:00:00
abstract::Langridge, Patricia (Oregon State University, Corvallis), and Richard Y. Morita. Thermolability of malic dehydrogenase from the obligate psychrophile Vibrio marinus. J. Bacteriol. 92:418-423. 1966.-The thermolability of malic dehydrogenase in whole cells of Vibrio marinus MP-1 grown at 15 C was compared with that of c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.2.418-423.1966
更新日期:1966-08-01 00:00:00
abstract::The dimorphic fungus Mucor racemosus was found to contain the cyclic nucleotide guanosine 3',5'-monophosphate (cGMP). Approximately equivalent amounts of the compound were found in ungerminated spores, yeastlike cells, and mycelia. Germinating spores contained severalfold higher amounts of cGMP than the other cell for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.1226-1228.1976
更新日期:1976-03-01 00:00:00
abstract::Inhibition of deoxyribonucleic acid (DNA) synthesis in Escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnaE and dnaB) or initiation (dnaA) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.1.107-113.1977
更新日期:1977-04-01 00:00:00
abstract::Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.2.508-512.2000
更新日期:2000-01-01 00:00:00
abstract::The nucleotide sequences of the Rhodobacter sphaeroides hemA and hemT genes, encoding 5-aminolevulinic acid (ALA) synthase isozymes, were determined. ALA synthase catalyzes the condensation of glycine and succinyl coenzyme A, the first and rate-limiting step in tetrapyrrole biosynthesis. The hemA and hemT structural g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.8.2292-2303.1993
更新日期:1993-04-01 00:00:00
abstract::Extracellular muramidase-2 of Enterococcus hirae ATCC 9790 was purified to homogeneity by substrate binding, guanidine-HCl extraction, and reversed-phase chromatography. A monoclonal antibody, 2F8, which specifically recognizes muramidase-2, was used to screen a genomic library of E. hirae ATCC 9790 DNA in bacteriopha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.5.1619-1625.1992
更新日期:1992-03-01 00:00:00
abstract::Sulfate-utilizing revertants of a cysteine auxotroph of Salmonella pullorum have been found that cause an accumulation in growth medium of a sulfur compound more oxidized than sulfide. The sulfur compound was presumptively identified as sulfite by the formation of a colored complex in the presence of basic fuchsin and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.365-369.1969
更新日期:1969-10-01 00:00:00
abstract::The parDE operon, located within the 3.2-kb stabilization region of plasmid RK2, encodes antitoxin (ParD) and toxin (ParE) proteins that stabilize the maintenance of this broad-host-range plasmid via a postsegregational killing mechanism. A ParE protein derivative, designated ParE', was purified by construction of a f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.5.1420-1429.1996
更新日期:1996-03-01 00:00:00
abstract::Bacillus thuringiensis var. thuringiensis Berliner grown at 14 C on a chemically defined liquid medium slowly produced spores and parasporal inclusions. Sporulation ultimately attained 50 to 100% of that of cultures grown at 30 C. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.445-448.1971
更新日期:1971-01-01 00:00:00
abstract::Biogenesis of the outer membrane (OM) is an essential process in gram-negative bacteria. One of the key steps of OM biogenesis is the assembly of integral outer membrane beta-barrel proteins (OMPs) by a protein machine called the Bam complex. In Escherichia coli, the Bam complex is composed of the essential proteins B...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06426-11
更新日期:2012-03-01 00:00:00
abstract::Streptococcus oralis, a commensal species of the human oral cavity, belongs to the Mitis group of streptococci, which includes one of the major human pathogens as well, S. pneumoniae. We report here the first complete genome sequence of this species. S. oralis Uo5, a high-level penicillin- and multiple-antibiotic-resi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00321-11
更新日期:2011-06-01 00:00:00
abstract::The rcs phosphorelay pathway components were originally identified as regulators of capsule synthesis. In addition to the transmembrane sensor kinase RcsC, the RcsA coregulator, and the response regulator RcsB, two new components have been characterized, RcsD and RcsF. RcsD, the product of the yojN gene, now renamed r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.19.6770-6778.2005
更新日期:2005-10-01 00:00:00
abstract::Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02169-12
更新日期:2013-07-01 00:00:00
abstract::Twenty-six FliF monomers assemble into the MS ring, a central motor component of the bacterial flagellum that anchors the structure in the inner membrane. Approximately 100 amino acids at the C terminus of FliF are exposed to the cytoplasm and, through the interaction with the FliG switch protein, a component of the f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1624-1633.2003
更新日期:2003-03-01 00:00:00
abstract::The regulation of the asparagine synthetase A gene of Escherichia coli was studied in vitro with a coupled transcription-translation system. It was shown that the 17-kilodalton gene, which is transcribed divergently from the adjacent asnA gene, codes for an activator of asnA transcription. The synthesis of the 17-kilo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.310-315.1985
更新日期:1985-10-01 00:00:00
abstract::Several enzymes and metabolites concerned with carbohydrate metabolism were examined in mice infected with Listeria monocytogenes. Liver glycogen and glucose decreased parallel to severity of infection. The concentration of glucose in the blood fell to abnormally low levels with a hypoglycemia being most evident at 72...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.2.538-543.1967
更新日期:1967-02-01 00:00:00
abstract::Escherichia coli contains 30 two-component systems (TCSs), each consisting of a histidine kinase and a response regulator. Whereas most TCSs are well characterized in this model organism, little is known about the YpdA/YpdB system. To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02051-12
更新日期:2013-02-01 00:00:00
abstract::Phosphatidylglycerol, the most abundant acidic phospholipid in Escherichia coli, is considered to play specific roles in various cellular processes that are essential for cell viability. A null mutation of pgsA, which encodes phosphatidylglycerophosphate synthase, does indeed confer lethality. However, pgsA null mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.19.5418-5425.2002
更新日期:2002-10-01 00:00:00
abstract::Twelve mutants of Escherichia coli K-12 have been isolated which carry chromosomal mutations that exhibit pleiotropic effects on the expression of F factor tra cistrons. F pilus synthesis, deoxyribonucleic acid transfer, and surface exclusion are all inhibited. Six of the mutants carry sfrA mutations, and six carry sf...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.730-737.1979
更新日期:1979-09-01 00:00:00
abstract::Burkholderia thailandensis is a soil saprophyte that is closely related to the pathogen Burkholderia pseudomallei, the etiological agent of melioidosis in humans. The environmental niches and infection sites occupied by these bacteria are thought to contain only limited concentrations of oxygen, where they can generat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00304-20
更新日期:2020-11-04 00:00:00
abstract::The mocC gene encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequence level to mas1' encoded by the T region of this plasmid. While Mas1 is required for the synthesis of mannopine (MOP) by crown gall tumor cells, MocC is essential for the utilization of MOP by Agrobacteriu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.11.3285-3292.1996
更新日期:1996-06-01 00:00:00
abstract::The actinomycin synthetases ACMS I, II, and III catalyze the assembly of the acyl peptide lactone precursor of actinomycin by a nonribosomal mechanism. We have cloned the genes of ACMS I (acmA) and ACMS II (acmB) by hybridization screening of a cosmid library of Streptomyces chrysomallus DNA with synthetic oligonucleo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.9.2468-2474.1998
更新日期:1998-05-01 00:00:00