Complementation analysis of the cold-sensitive phenotype of the Escherichia coli csdA deletion strain.

abstract：:Two proteins of Escherichia coli, termed Wzc and Wzb, were analyzed for their capacity to participate in the reversible phosphorylation of proteins on tyrosine. First, Wzc was overproduced from its specific gene and purified to homogeneity by affinity chromatography. Upon incubation in the presence of radioactive ATP,...

journal_title：Journal of bacteriology

authors： Vincent C,Doublet P,Grangeasse C,Vaganay E,Cozzone AJ,Duclos B

更新日期：1999-06-01 00:00:00

abstract：:To identify the genes in coliphage 186 that are required for lysogeny, we isolated clear-plaque mutants. Complementation studies and DNA sequencing identified two genes, the cI gene for the immunity maintenance repressor and the cII gene, which is required only for the establishment of lysogeny. One mutant carried a c...

journal_title：Journal of bacteriology

authors： Lamont I,Richardson H,Carter DR,Egan JB

更新日期：1993-08-01 00:00:00

abstract：:DNA sequence analysis of a region of the Streptomyces sp. strain C5 daunomycin biosynthesis gene cluster, located between the daunomycin polyketide biosynthesis gene cluster and a dnrI (transcriptional activator) homolog, revealed the presence of a gene encoding a P-450-like enzyme with a deduced Mr of 46,096. Express...

journal_title：Journal of bacteriology

authors： Dickens ML,Strohl WR

更新日期：1996-06-01 00:00:00

abstract：:Macrocysts have been found among strains of Dictyostelium rosarium Raper and Cavender (K. B. Raper and J. C. Cavender, J. Elisha Mitchell Sci. Soc. 84:31-47, 1968). Strains that exhibit mating capabilities could be classified into one of three mating types: A1, A2, or A3. Each mating type was self-incompatible but cro...

journal_title：Journal of bacteriology

authors： Chang MT,Raper KB

更新日期：1981-09-01 00:00:00

abstract：:The symbiotic interaction between Rhizobium etli and Phaseolus vulgaris, the common bean plant, ultimately results in the formation of nitrogen-fixing nodules. Many aspects of the intermediate and late stages of this interaction are still poorly understood. The R. etli relA gene was identified through a genome-wide sc...

journal_title：Journal of bacteriology

authors： Moris M,Braeken K,Schoeters E,Verreth C,Beullens S,Vanderleyden J,Michiels J

更新日期：2005-08-01 00:00:00

abstract：:Nine acetotrophic and three methylotrophic strains of methane-producing bacteria were screened for the presence of plasmid DNA. Plasmids were detected in three marine isolates, including Methanosarcina acetivorans. All three plasmids appeared to be similar based on size and restriction site analyses. The plasmid from ...

journal_title：Journal of bacteriology

authors： Sowers KR,Gunsalus RP

更新日期：1988-10-01 00:00:00

abstract：:Intracellular accumulation of nitrate, indicative of the operation of an active nitrate transport system, has been measured in intact cells of the cyanobacterium Anacystis nidulans. The ability of the cells to accumulate nitrate was effectively hindered by either ammonium addition or selective inhibition of CO2 fixati...

journal_title：Journal of bacteriology

authors： Lara C,Romero JM,Guerrero MG

更新日期：1987-09-01 00:00:00

abstract：:Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the "downhill" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhyd...

journal_title：Journal of bacteriology

authors： Cecchini G,Koch AL

更新日期：1975-07-01 00:00:00

abstract：:pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...

journal_title：Journal of bacteriology

authors： Zotchev SB,Schrempf H,Hutchinson CR

更新日期：1995-08-01 00:00:00

abstract：:Cryptococcus neoformans is an important pathogenic fungus that has been classified as a basidiomycete. Little is known of the molecular genetics of this fungal pathogen. To begin such studies, we devised a procedure for extraction of DNA from cryptococci; this method involved the use of the cell wall-active enzyme Nov...

journal_title：Journal of bacteriology

authors： Restrepo BI,Barbour AG

更新日期：1989-10-01 00:00:00

abstract：:We have cloned the palA gene of Aspergillus nidulans, one of six genes participating in ambient pH signal transduction in a regulatory circuit mediating pH regulation of gene expression. The derived 798-residue PalA protein is 29.4% identical over its entire length to a hypothetical protein from the nematode Caenorhab...

journal_title：Journal of bacteriology

authors： Negrete-Urtasun S,Denison SH,Arst HN Jr

更新日期：1997-03-01 00:00:00

abstract：:The bacterial plant pathogen Pseudomonas syringae depends on a type III protein secretion system and the effector proteins that it translocates into plant cells to cause disease and to elicit the defense-associated hypersensitive response on resistant plants. The availability of the P. syringae pv. tomato DC3000 genom...

journal_title：Journal of bacteriology

authors： Wehling MD,Guo M,Fu ZQ,Alfano JR

更新日期：2004-06-01 00:00:00

abstract：:Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...

journal_title：Journal of bacteriology

authors： Zhao N,Bai Y,Zhao XQ,Yang ZY,Bai FW

更新日期：2012-12-01 00:00:00

abstract：:Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strai...

journal_title：Journal of bacteriology

authors： Hinnebusch J,Barbour AG

更新日期：1992-08-01 00:00:00

abstract：:Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a f...

journal_title：Journal of bacteriology

authors： Burke WF Jr,Slinker BS

更新日期：1982-02-01 00:00:00

abstract：:The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo rep...

journal_title：Journal of bacteriology

authors： Wray LV Jr,Reznikoff WS

更新日期：1983-12-01 00:00:00

abstract：:Spx is a global regulator that is widespread among the low-G+C-content gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under nonstress conditions, Spx is rapidly degraded by the ClpXP protease...

journal_title：Journal of bacteriology

authors： Engman J,Rogstam A,Frees D,Ingmer H,von Wachenfeldt C

更新日期：2012-03-01 00:00:00

abstract：:The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped co...

journal_title：Journal of bacteriology

authors： Sherman MB,Orlova EV,Smirnova EA,Hovmöller S,Zorin NA

更新日期：1991-04-01 00:00:00

abstract：:During growth of streptomycin-dependent strains of Escherichia coli in the absence of streptomycin (deprived growth), both constitutive and inducible synthesis of beta-galactosidase were preferentially inhibited. A similar preferential inhibition of constitutive and derepressed synthesis of alkaline phosphatase was ob...

journal_title：Journal of bacteriology

authors： Goodman RE,Spotts CR

更新日期：1967-10-01 00:00:00

abstract：:Little is known about the effect of the host on the genetic stability of bacterial plant pathogens. Crown gall, a plant disease caused by Agrobacterium tumefaciens, may represent a useful model to study this effect. Indeed, our previous observations on the natural occurrence and origin of nonpathogenic agrobacteria su...

journal_title：Journal of bacteriology

authors： Bélanger C,Canfield ML,Moore LW,Dion P

更新日期：1995-07-01 00:00:00

abstract：:The deoxyribonucleic acid base composition (percent guanine + cytosine [GC]) was determined for 29 strains, representing 18 species of the genus Kluyveromyces. It was concluded that on the basis of GC content (47.4%) and other properties K. veronae occupies an uncertain position in the genus Kluyveromyces. The GC cont...

journal_title：Journal of bacteriology

authors： Martini A,Phaff HJ,Douglass SA

更新日期：1972-08-01 00:00:00

abstract：:In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...

journal_title：Journal of bacteriology

authors： Myers CR,Myers JM

更新日期：1992-06-01 00:00:00

abstract：:A positive selection method was used to isolate four Saccharomyces cerevisiae mutations that cause derepressed synthesis of asparaginase II. The four mutations (and1, and2, and3, and4) were neither closely linked to each other nor linked to previously characterized mutations (asp3, asp6) which cause the complete loss ...

journal_title：Journal of bacteriology

authors： Kamerud JQ,Roon RJ

更新日期：1986-01-01 00:00:00

abstract：:The imp genes, responsible for the UV protection and mutation effects of the I incompatibility group plasmid TP110, have been cloned into vector plasmids, and their products have been analyzed. The genetic information required for expression of these properties was carried in a continuous DNA sequence of approximately...

journal_title：Journal of bacteriology

authors： Glazebrook JA,Grewal KK,Strike P

更新日期：1986-10-01 00:00:00

abstract：:A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is...

journal_title：Journal of bacteriology

authors： Turinsky AJ,Moir-Blais TR,Grundy FJ,Henkin TM

更新日期：2000-10-01 00:00:00

abstract：:Uracil-DNA glycosylase activity was found in Streptococcus pneumoniae, and the enzyme was partially purified. An ung mutant lacking the activity was obtained by positive selection of cells transformed with a plasmid containing uracil in its DNA. The effects of the ung mutation on mutagenic processes in S. pneumoniae w...

journal_title：Journal of bacteriology

authors： Chen JD,Lacks SA

更新日期：1991-01-01 00:00:00

abstract：:In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...

journal_title：Journal of bacteriology

authors： Vartak NB,Liu L,Wang BM,Berg CM

更新日期：1991-06-01 00:00:00

abstract：:The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli...

journal_title：Journal of bacteriology

authors： Nelson DR,Rittenberg SC

更新日期：1981-09-01 00:00:00

abstract：:Mycoplasmas that are known to exhibit gliding motility possess a differentiated tip structure. This polar organelle mediates cytadherence and gliding motor activity and contains a cytoskeleton-like component that provides structural support. Here, we describe gliding motility and a unique cytoskeleton in Mycoplasma in...

journal_title：Journal of bacteriology

authors： Relich RF,Friedberg AJ,Balish MF

更新日期：2009-08-01 00:00:00

abstract：:The obligate intracellular pathogen Chlamydia trachomatis expresses a type III secretion system (T3SS) which has the potential to contribute significantly to pathogenesis. Based on a demonstrated role of type III secretion (T3S)-specific chaperones in the secretion of antihost proteins by gram-negative pathogens, we i...

journal_title：Journal of bacteriology

authors： Fields KA,Fischer ER,Mead DJ,Hackstadt T

更新日期：2005-09-01 00:00:00