Linear- and circular-plasmid copy numbers in Borrelia burgdorferi.

Abstract:

:Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strain, B31, of B. burgdorferi. All three plasmids were present in low copy number, about one per chromosome equivalent, as determined by relative hybridizations of replicon-specific DNA probes. The low copy number of Borrelia plasmids suggests that initiation of DNA replication and partitioning are carefully controlled during the cell division cycle. The copy numbers of these three plasmids of strain B31 were unchanged after approximately 7,000 generations in continuous in vitro culture. A clone of B. burgdorferi B31 that did not contain the 16-kb linear plasmid was obtained after exposure of a culture to novobiocin, a DNA gyrase inhibitor. The plasmid-cured strain contains only one linear plasmid, the 49-kb plasmid, and thus has the smallest genome reported to date for B. burgdorferi.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hinnebusch J,Barbour AG

doi

10.1128/jb.174.16.5251-5257.1992

keywords:

subject

Has Abstract

pub_date

1992-08-01 00:00:00

pages

5251-7

issue

16

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

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