Linear- and circular-plasmid copy numbers in Borrelia burgdorferi.


:Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strain, B31, of B. burgdorferi. All three plasmids were present in low copy number, about one per chromosome equivalent, as determined by relative hybridizations of replicon-specific DNA probes. The low copy number of Borrelia plasmids suggests that initiation of DNA replication and partitioning are carefully controlled during the cell division cycle. The copy numbers of these three plasmids of strain B31 were unchanged after approximately 7,000 generations in continuous in vitro culture. A clone of B. burgdorferi B31 that did not contain the 16-kb linear plasmid was obtained after exposure of a culture to novobiocin, a DNA gyrase inhibitor. The plasmid-cured strain contains only one linear plasmid, the 49-kb plasmid, and thus has the smallest genome reported to date for B. burgdorferi.


J Bacteriol


Journal of bacteriology


Hinnebusch J,Barbour AG





Has Abstract


1992-08-01 00:00:00












  • Effective symbiosis between Rhizobium etli and Phaseolus vulgaris requires the alarmone ppGpp.

    abstract::The symbiotic interaction between Rhizobium etli and Phaseolus vulgaris, the common bean plant, ultimately results in the formation of nitrogen-fixing nodules. Many aspects of the intermediate and late stages of this interaction are still poorly understood. The R. etli relA gene was identified through a genome-wide sc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Moris M,Braeken K,Schoeters E,Verreth C,Beullens S,Vanderleyden J,Michiels J

    更新日期:2005-08-01 00:00:00

  • Crystal structure of pyridoxal kinase from the Escherichia coli pdxK gene: implications for the classification of pyridoxal kinases.

    abstract::The pdxK and pdxY genes have been found to code for pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway. Two pyridoxal kinase structures have recently been published, including Escherichia coli pyridoxal kinase 2 (ePL kinase 2) and sheep pyridoxal kinase, products of the pdxY and pdxK genes,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Safo MK,Musayev FN,di Salvo ML,Hunt S,Claude JB,Schirch V

    更新日期:2006-06-01 00:00:00

  • Genome sequence of Reyranella massiliensis, a bacterium associated with amoebae.

    abstract::Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pagnier I,Croce O,Robert C,Raoult D,La Scola B

    更新日期:2012-10-01 00:00:00

  • Identification of the origin of transfer (oriT) and DNA relaxase required for conjugation of the integrative and conjugative element ICEBs1 of Bacillus subtilis.

    abstract::Integrative and conjugative elements (ICEs), also known as conjugative transposons, are mobile genetic elements that can transfer from one bacterial cell to another by conjugation. ICEBs1 is integrated into the trnS-leu2 gene of Bacillus subtilis and is regulated by the SOS response and the RapI-PhrI cell-cell peptide...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lee CA,Grossman AD

    更新日期:2007-10-01 00:00:00

  • Medium-dependent variation of deoxyribonucleic acid segregation in Escherichia coli.

    abstract::The degree to which deoxyribonucleic acid segregates nonrandomly has been investigated for Escherichia coli B/r growing in different media. The degree of nonrandom segregation observed is dependent on the medium, with segregation becoming less random as the growth rate decreases. This indicates that there must be some...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cooper S,Weinberger M

    更新日期:1977-04-01 00:00:00

  • Platinum-induced filamentous growth in Escherichia coli.

    abstract::Certain group VIIIB transition metal compounds were found to inhibit cell division in Escherichia coli, causing marked filamentous growth. Gram-negative bacilli were the most sensitive to this effect, whereas gram-positive bacilli responded only at near-toxic levels of the metal. None of the cocci tested showed any ap...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rosenberg B,Renshaw E,Vancamp L,Hartwick J,Drobnik J

    更新日期:1967-02-01 00:00:00

  • Anaerobic growth yields of Aerobacter cloacae and Escherichia coli.

    abstract::Aerobacter cloacae UW-C83 and Escherichia coli K-12 were grown under various anaerobic environments. Y(atp) values were calculated by determination of cell weights and analyses for fermentation products. These Y(atp) values are compared with others reported in the literature. Limitation of growth by factors other than...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hernandez E,Johnson MJ

    更新日期:1967-10-01 00:00:00

  • Neutralization of endotoxin toxicity in chick embryos by antibiotics.

    abstract::Rifkind, David (University of Colorado Medical Center, Denver), and John D. Palmer. Neutralization of endotoxin toxicity in chick embryos by antibiotics. J. Bacteriol. 92:815-819. 1966.-Three cationic cyclic polypeptide antibiotics, polymyxin B sulfate colistin sulfate, and tyrocidine hydrochloride, were shown to neut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rifkind D,Palmer JD

    更新日期:1966-10-01 00:00:00

  • Mutations at several loci cause increased expression of ribonucleotide reductase in Escherichia coli.

    abstract::Production of deoxyribonucleotides for DNA synthesis is an essential and tightly regulated process. The class Ia ribonucleotide reductase (RNR), the product of the nrdAB genes, is required for aerobic growth of Escherichia coli. In catalyzing the reduction of ribonucleotides, two of the cysteines of RNR become oxidize...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Feeney MA,Ke N,Beckwith J

    更新日期:2012-03-01 00:00:00

  • Global mutational analysis of NtrC-like activators in Myxococcus xanthus: identifying activator mutants defective for motility and fruiting body development.

    abstract::The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Caberoy NB,Welch RD,Jakobsen JS,Slater SC,Garza AG

    更新日期:2003-10-01 00:00:00

  • Physical properties of L-asparaginase from Serratia marcescens.

    abstract::Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stern ML,Phillips AW,Gottlieb AJ

    更新日期:1976-02-01 00:00:00

  • nodO, a new nod gene of the Rhizobium leguminosarum biovar viciae sym plasmid pRL1JI, encodes a secreted protein.

    abstract::The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: de Maagd RA,Wijfjes AH,Spaink HP,Ruiz-Sainz JE,Wijffelman CA,Okker RJ,Lugtenberg BJ

    更新日期:1989-12-01 00:00:00

  • Crystal structure of the cystic fibrosis transmembrane conductance regulator inhibitory factor Cif reveals novel active-site features of an epoxide hydrolase virulence factor.

    abstract::Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bahl CD,Morisseau C,Bomberger JM,Stanton BA,Hammock BD,O'Toole GA,Madden DR

    更新日期:2010-04-01 00:00:00

  • Proteins induced in Escherichia coli by benzoic acid.

    abstract::Proteins induced by benzoic acid in Escherichia coli were observed on two-dimensional electrophoretic gels (2-D gels). Cultures were grown in glucose-rich medium in the presence or absence of 20 mM benzoate at an external pH of 6.5, where the pH gradient (deltapH) is large and benzoate accumulates, and at an external ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lambert LA,Abshire K,Blankenhorn D,Slonczewski JL

    更新日期:1997-12-01 00:00:00

  • Genetic analysis of pathway specificity during posttranslational protein translocation across the Escherichia coli plasma membrane.

    abstract::In Escherichia coli, the SecB/SecA branch of the Sec pathway and the twin-arginine translocation (Tat) pathway represent two alternative possibilities for posttranslational translocation of proteins across the cytoplasmic membrane. Maintenance of pathway specificity was analyzed using a model precursor consisting of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Blaudeck N,Kreutzenbeck P,Freudl R,Sprenger GA

    更新日期:2003-05-01 00:00:00

  • Evidence that specific and "general" control of ornithine carbamoyltransferase production occurs at the level of transcription in Saccharomyces cerevisiae.

    abstract::Ornithine carbamoyltransferase synthesis is subject to two major regulatory systems in Saccharomyces cerevisiae. One system is specific for the arginine biosynthetic enzymes, whereas the other appears to be general, acting on a variety of other amino acid pathways as well. We observed that the synthetic capacity for c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Messenguy F,Cooper TG

    更新日期:1977-06-01 00:00:00

  • Site-directed mutagenesis in Escherichia coli of a stable R772::Ti cointegrate plasmid from Agrobacterium tumefaciens.

    abstract::The host range of an octopine Ti plasmid is limited to Rhizobiaceae. This has been extended also to Escherichia coli in the form of a stable cointegrate with the wide-host-range plasmid R772. Its structure was studied by constructing a physical map of R772 and of the R772::pTiB6 cointegrate. An insertion sequence pres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hille J,van Kan J,Klasen I,Schilperoort R

    更新日期:1983-05-01 00:00:00

  • Relative Rates of Amino Acid Import via the ABC Transporter GlnPQ Determine the Growth Performance of Lactococcus lactis.

    abstract:UNLABELLED:The GlnPQ transporter from Lactococcus lactis has the remarkable feature of having two substrate-binding domains (SBDs) fused to the N terminus of the transmembrane domain (TMD), and thus four SBDs are present in the homodimeric complex. Although X-ray structures and ligand binding data are available for bot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fulyani F,Schuurman-Wolters GK,Slotboom DJ,Poolman B

    更新日期:2015-11-09 00:00:00

  • Role of EscP (Orf16) in injectisome biogenesis and regulation of type III protein secretion in enteropathogenic Escherichia coli.

    abstract::Enteropathogenic Escherichia coli employs a type III secretion system (T3SS) to translocate virulence effector proteins directly into enterocyte host cells, leading to diarrheal disease. The T3SS is encoded within the chromosomal locus of enterocyte effacement (LEE). The function of some of the LEE-encoded proteins re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Monjarás Feria J,García-Gómez E,Espinosa N,Minamino T,Namba K,González-Pedrajo B

    更新日期:2012-11-01 00:00:00


    abstract::Deibel, R. H. (American Meat Institute Foundation, Chicago, Ill.). Hydrolysis of proteins and nucleic acids by Lancefield group A and other streptococci. J. Bacteriol. 86:1270-1274. 1963.-Classically, group A streptococci have been considered to be nonproteolytic in spite of the observation that a proteinase is produc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: DEIBEL RH

    更新日期:1963-12-01 00:00:00

  • Mutants of Escherichia coli with a growth requirement for either lysine or pyridoxine.

    abstract::Two distinct phenotypic classes of lysine requiring auxotrophs of Escherichia coli are described. Mutants of the LysA class produce little or no active diaminopimelic acid (DAP) decarboxylase and specifically require lysine for growth. Mutants of the LysB class produce a cryptic DAP decarboxylase which can be activate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bukhari AI,Taylor AL

    更新日期:1971-03-01 00:00:00

  • Homology with a repeated Yersinia pestis DNA sequence IS100 correlates with pesticin sensitivity in Yersinia pseudotuberculosis.

    abstract::We have identified IS100 sequences in a specific subset of Yersinia pseudotuberculosis isolates that were also sensitive to the Y. pestis-produced bacteriocin, pesticin. In contrast, Y. pseudotuberculosis strains which did not contain IS100 sequences were not sensitive to pesticin. We propose that IS100 serves as a mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McDonough KA,Hare JM

    更新日期:1997-03-01 00:00:00

  • VirB3 to VirB6 and VirB8 to VirB11, but not VirB7, are essential for mediating persistence of Brucella in the reticuloendothelial system.

    abstract::The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: den Hartigh AB,Rolán HG,de Jong MF,Tsolis RM

    更新日期:2008-07-01 00:00:00

  • Characterization of the pH signal transduction pathway gene palA of Aspergillus nidulans and identification of possible homologs.

    abstract::We have cloned the palA gene of Aspergillus nidulans, one of six genes participating in ambient pH signal transduction in a regulatory circuit mediating pH regulation of gene expression. The derived 798-residue PalA protein is 29.4% identical over its entire length to a hypothetical protein from the nematode Caenorhab...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Negrete-Urtasun S,Denison SH,Arst HN Jr

    更新日期:1997-03-01 00:00:00

  • Role and specificity of plasmid RP4-encoded DNA primase in bacterial conjugation.

    abstract::The role of the DNA primase of IncP plasmids was examined with a derivative of RP4 containing Tn7 in the primase gene (pri). The mutant was defective in mediating bacterial conjugation, with the deficiency varying according to the bacterial strains used as donors and recipients. Complementation tests involving recombi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Merryweather A,Barth PT,Wilkins BM

    更新日期:1986-07-01 00:00:00

  • Regulation of folylpoly-gamma-glutamate synthesis in bacteria: in vivo and in vitro synthesis of pteroylpoly-gamma-glutamates by Lactobacillus casei and Streptococcus faecalis.

    abstract::Lactobacillus casei and Streptococcus faecalis accumulated labeled folic acid and metabolized this compound to poly-gamma-glutamates of chain lengths of up to 11 and 5, respectively. Octa- and nonaglutamates predominated in L. casei, and tetraglutamates predominated in S. faecalis. The most effective monoglutamate sub...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Shane B,Bognar AL,Goldfarb RD,LeBowitz JH

    更新日期:1983-01-01 00:00:00

  • Sequence diversity of Treponema pallidum subsp. pallidum tprK in human syphilis lesions and rabbit-propagated isolates.

    abstract::The tprK gene of Treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, belongs to a 12-member gene family and encodes a protein with a predicted cleavable signal sequence and predicted transmembrane domains. Except for the Nichols type strain, all rabbit-propagated isolates of T. pallidum exami...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: LaFond RE,Centurion-Lara A,Godornes C,Rompalo AM,Van Voorhis WC,Lukehart SA

    更新日期:2003-11-01 00:00:00

  • A Salmonella typhimurium mutant dependent upon carbamyl aspartate for resistance to 5-fluorouracil is specifically affected in ubiquinone biosynthesis.

    abstract::The isolation and properties of a mutant dependent upon exogenous carbamyl aspartate for resistance to 5-fluorouracil are described. The mutant was deficient in the synthesis of ubiquinone and accumulated a quinone provisionally identified as the ubiquinone precursor 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zak VL,Kelln RA

    更新日期:1981-02-01 00:00:00

  • Regulation of the SOS response analyzed by RecA protein amplification.

    abstract::A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variation...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Calsou P,Salles B

    更新日期:1985-06-01 00:00:00

  • Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.

    abstract::Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI end...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: de Graaff J,Crosa JH,Heffron F,Falkow S

    更新日期:1978-06-01 00:00:00