Activation of inactive nitrogenase by acid-treated component I.

Abstract:

:When Azotobacter vinelandii was derepressed for nitrogenase synthesis in a N-free medium containing tungstate instead of molybdate, an inactive component I was synthesized. Although this inactive component I could be activated in vivo upon addition of molybdate to the medium, it could not be activated in vitro when molybdate was added to the extracts. Activation occurred, however, when an acid-treated component I was added to extracts of cells derepressed in medium containing tungstate. Acid treatment completely abolished component I activity. Mutant strains UW45 and UW10 were unable to fix N(2). Both strains synthesized normal levels of component II but produced inactive component I. Acid-treated component I activated inactive component I in extracts of mutant strain UW45 but not mutant strain UW10. This activating factor could be obtained from N(2)-fixing Klebsiella pneumoniae, Clostridium pasteurianum, and Rhodospirillum rubrum.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Nagatani HH,Shah VK,Brill WJ

doi

10.1128/JB.120.2.697-701.1974

subject

Has Abstract

pub_date

1974-11-01 00:00:00

pages

697-701

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

120

pub_type

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