Antisense RNA regulation by stable complex formation in the Enterococcus faecalis plasmid pAD1 par addiction system.

Abstract:

:The par stability determinant, encoded by the Enterococcus faecalis plasmid pAD1, is the only antisense RNA regulated postsegregational killing system identified in gram-positive bacteria. Because of the unique organization of the par locus, the par antisense RNA, RNA II, binds to its target, RNA I, at relatively small, interspersed regions of complementarity. The results of this study suggest that, rather than targeting the antisense bound message for rapid degradation, as occurs in most other antisense RNA regulated systems, RNA I and RNA II form a relatively stable, presumably translationally inactive complex. The stability of the RNA I-RNA II complex would allow RNA I to persist in an untranslated state unless or until the encoding plasmid was lost. After plasmid loss, RNA II would be removed from the complex, allowing translational activation of RNA I. The mechanism of RNA I activation in vivo is unknown, but in vitro dissociation experiments suggest that active removal of RNA II, for example by a cellular RNase, may be required.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Weaver KE,Ehli EA,Nelson JS,Patel S

doi

10.1128/JB.186.19.6400-6408.2004

keywords:

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

6400-8

issue

19

eissn

0021-9193

issn

1098-5530

pii

186/19/6400

journal_volume

186

pub_type

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