Streptococcus faecium ATCC 9790 penicillin-binding proteins and penicillin sensitivity are heavily influenced by growth conditions: proposal for an indirect mechanism of growth inhibition by beta-lactams.

abstract：:Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation. ...

journal_title：Journal of bacteriology

authors： Nouwen N,Driessen AJ

更新日期：2005-10-01 00:00:00

abstract：:Temperature-resistant pseudorevertants of the temperature-sensitive Escherichia coli mutant KNS19, harboring a mutation in rplX, the gene for ribosomal protein L24, were isolated, cloned, and sequenced. The codon GAC for the amino acid Asp in the temperature-sensitive mutant corresponding to position 84 in the protein...

journal_title：Journal of bacteriology

authors： Nishi K,Müller M,Schnier J

更新日期：1987-10-01 00:00:00

abstract：:The Salmonella plasmid virulence spvABCD genes are growth phase regulated and require RpoS for maximal expression in stationary phase. We identified a growth phase-independent expression of spv which is mediated by short-chain fatty acids. During this fatty acid-mediated expression of spv, RpoS is required for inducti...

journal_title：Journal of bacteriology

authors： El-Gedaily A,Paesold G,Chen CY,Guiney DG,Krause M

更新日期：1997-02-01 00:00:00

abstract：:Mycoplasma penetrans is a newly identified species of the genus MYCOPLASMA: It was first isolated from a urine sample from a human immunodeficiency virus (HIV)-infected patient. M. penetrans changes its surface antigen profile with high frequency. The changes originate from ON<==>OFF phase variations of the P35 family...

journal_title：Journal of bacteriology

authors： Horino A,Sasaki Y,Sasaki T,Kenri T

更新日期：2003-01-01 00:00:00

abstract：:Genetic diversity and relationships in 74 Helicobacter pylori isolates recovered from patients assigned to distinct clinical categories were estimated by examination of allelic variation in six genes encoding metabolic housekeeping enzymes by multilocus enzyme electrophoresis. Seventy-three distinct allele profiles, r...

journal_title：Journal of bacteriology

authors： Go MF,Kapur V,Graham DY,Musser JM

更新日期：1996-07-01 00:00:00

abstract：:The ompB operon encodes OmpR and EnvZ, two proteins that are necessary for the expression and osmoregulation of the OmpF and OmpC porins in Escherichia coli. We have used in vitro and in vivo experiments to show that cyclic AMP and the cyclic AMP receptor protein (CRP) directly regulate ompB. ompB expression in an omp...

journal_title：Journal of bacteriology

authors： Huang L,Tsui P,Freundlich M

更新日期：1992-02-01 00:00:00

abstract：:A total of 508 random clones from five Mycoplasma genitalium genomic libraries were partially sequenced and analyzed. This resulted in the identification of 291 unique contigs. Sequence information from these clones (100,993 nucleotides), representing approximately 17% of this pathogen's genome, was analyzed by compar...

journal_title：Journal of bacteriology

authors： Peterson SN,Hu PC,Bott KF,Hutchison CA 3rd

更新日期：1993-12-01 00:00:00

abstract：:Cyclic 2,3-diphosphoglycerate synthetase (cDPGS) catalyzes the synthesis of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond in 2,3-diphosphoglycerate. cDPG is known to be accumulated to high intracellular concentrations (>300 mM) as a putative thermoadapter in some hyperthe...

journal_title：Journal of bacteriology

authors： Matussek K,Moritz P,Brunner N,Eckerskorn C,Hensel R

更新日期：1998-11-01 00:00:00

abstract：:Specific labeling of a single row of cellulose-synthesizing complexes (terminal complexes, TC subunits, TCs, or TC arrays) in Acetobacter xylinum by antibodies raised against a 93-kDa protein (the cyclic dignanylic acid-binding protein) has been demonstrated by using the sodium dodecyl sulfate (SDS)-freeze-fracture la...

journal_title：Journal of bacteriology

authors： Kimura S,Chen HP,Saxena IM,Brown RM Jr,Itoh T

更新日期：2001-10-01 00:00:00

abstract：:Two sets of genes for the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) were detected in the photosynthetic purple sulfur bacterium Chromatium vinosum by hybridization analysis with RuBisCO gene probes, cloned by using the lambda Fix vector, and designated rbcL-rbcS and rbcA-rbc...

journal_title：Journal of bacteriology

authors： Viale AM,Kobayashi H,Akazawa T

更新日期：1989-05-01 00:00:00

abstract：:Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a f...

journal_title：Journal of bacteriology

authors： Burke WF Jr,Slinker BS

更新日期：1982-02-01 00:00:00

abstract：:Bacterial cell division is mediated by a set of proteins that assemble to form a large multiprotein complex called the divisome. Recent studies in Bacillus subtilis and Escherichia coli indicate that cell division proteins are involved in multiple cooperative binding interactions, thus presenting a technical challenge...

journal_title：Journal of bacteriology

authors： Robichon C,King GF,Goehring NW,Beckwith J

更新日期：2008-09-01 00:00:00

abstract：:We have reinvestigated the genetic organization and the transcription regulation of the dsd operon of Escherichia coli. By combining genetic and biochemical studies, it is demonstrated that the regulatory region of the operon and the gene encoding the specific regulator of D-serine tolerance (dsdC) had been misplaced ...

journal_title：Journal of bacteriology

authors： Nørregaard-Madsen M,McFall E,Valentin-Hansen P

更新日期：1995-11-01 00:00:00

abstract：:The elongation factor Tu, encoded by tuf genes, is a GTP binding protein that plays a central role in protein synthesis. One to three tuf genes per genome are present, depending on the bacterial species. Most low-G+C-content gram-positive bacteria carry only one tuf gene. We have designed degenerate PCR primers derive...

journal_title：Journal of bacteriology

authors： Ke D,Boissinot M,Huletsky A,Picard FJ,Frenette J,Ouellette M,Roy PH,Bergeron MG

更新日期：2000-12-01 00:00:00

abstract：:The 3.1-Mb genome of an outbreak methicillin-resistant Staphylococcus aureus (MRSA) strain (TW20) contains evidence of recently acquired DNA, including two large regions (635 kb and 127 kb). The strain is resistant to a wide range of antibiotics, antiseptics, and heavy metals due to resistance genes encoded on mobile ...

journal_title：Journal of bacteriology

authors： Holden MT,Lindsay JA,Corton C,Quail MA,Cockfield JD,Pathak S,Batra R,Parkhill J,Bentley SD,Edgeworth JD

更新日期：2010-02-01 00:00:00

abstract：:The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...

journal_title：Journal of bacteriology

authors： Kochan I,Golden CA,Bukovic JA

更新日期：1969-10-01 00:00:00

abstract：:DNA sequence analysis of a region of the Streptomyces sp. strain C5 daunomycin biosynthesis gene cluster, located between the daunomycin polyketide biosynthesis gene cluster and a dnrI (transcriptional activator) homolog, revealed the presence of a gene encoding a P-450-like enzyme with a deduced Mr of 46,096. Express...

journal_title：Journal of bacteriology

authors： Dickens ML,Strohl WR

更新日期：1996-06-01 00:00:00

abstract：:The Lyme disease spirochete Borrelia burgdorferi is unique among bacteria in its large number of lipoproteins that are encoded by a small, exceptionally fragmented, and predominantly linear genome. Peripherally anchored in either the inner or outer membrane and facing either the periplasm or the external environment, ...

journal_title：Journal of bacteriology

authors： Dowdell AS,Murphy MD,Azodi C,Swanson SK,Florens L,Chen S,Zückert WR

更新日期：2017-02-28 00:00:00

abstract：:The DNA binding protein WhiA is conserved in Gram-positive bacteria and is present in the genetically simple cell wall-lacking mycoplasmas. The protein shows homology to eukaryotic homing endonucleases but lacks nuclease activity. WhiA was first characterized in streptomycetes, where it regulates the expression of key...

journal_title：Journal of bacteriology

authors： Bohorquez LC,Surdova K,Jonker MJ,Hamoen LW

更新日期：2018-03-26 00:00:00

abstract：:Escherichia coli cells expressing the tpm gene encoding the bacterial thiopurine methyltransferase (bTPMT) are shown to methylate selenite and (methyl)selenocysteine into dimethylselenide (DMSe) and dimethyldiselenide (DMDSe). E. coli cells expressing tpm from a gene library cosmid clone (harboring a Pseudomonas syrin...

journal_title：Journal of bacteriology

authors： Ranjard L,Prigent-Combaret C,Nazaret S,Cournoyer B

更新日期：2002-06-01 00:00:00

abstract：:omega 4403 is the site of a Tn5 lac insertion in the Myxococcus xanthus genome that fuses lacZ expression to a developmentally regulated promoter. Cell-cell interactions that occur during development, including C-signaling, are required for expression of Tn5 lac omega 4403. We have cloned DNA upstream of the omega 440...

journal_title：Journal of bacteriology

authors： Fisseha M,Gloudemans M,Gill RE,Kroos L

更新日期：1996-05-01 00:00:00

abstract：:Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile and appear to influence toxin production. In Stickland reactions, pairs of amino acids donate and accept electrons, gene...

journal_title：Journal of bacteriology

authors： Bouillaut L,Self WT,Sonenshein AL

更新日期：2013-02-01 00:00:00

abstract：:A hydrocarbon-utilizing Brevibacterium which grew into the oil phase of an oil-water system was tested for survival at elevated temperature. Cells suspended in oil and cells that had been resuspended in aqueous solution were tested by placing 1-ml samples of the cell suspension in small test tubes immersed in a contro...

journal_title：Journal of bacteriology

authors： Severance MM,LaRock PA

更新日期：1973-12-01 00:00:00

abstract：:The high-potential 4Fe-4S* center ofAzotobacter vinelandii ferredoxin I has been titrated potentiometrically by a reductive procedure. The absorbance decrease at 510 nm accompanying the reduction of the high-potential center titrated with an Em of 320 mV (n = 1). The low-potential 4Fe-4S* center was titrated by using ...

journal_title：Journal of bacteriology

authors： Yoch DC,Carithers RP

更新日期：1978-11-01 00:00:00

abstract：:Since relatively few quantitative immunological studies had been conducted with invertebrates, this study was designed to learn more about the immune potential of the oyster, a molluscan representative. Experiments measured the primary and secondary clearance rates of T2 coliphage in the oyster. The fate of phage inje...

journal_title：Journal of bacteriology

authors： Acton RT,Evans EE

更新日期：1968-04-01 00:00:00

abstract：:From a lysogen with lambda integrated in the leu operon, specialized transducing phages that carry the cell division, murein biosynthesis, and envelope permeability genes located about 0.5 min to the right of leu were isolated. These phages were used to identify the previously undiscovered cell division gene sep. A ge...

journal_title：Journal of bacteriology

authors： Fletcher G,Irwin CA,Henson JM,Fillingim C,Malone MM,Walker JR

更新日期：1978-01-01 00:00:00

abstract：:Alpha granules were isolated from a blue-green alga, Nostoc muscorum, in large quantities and high purity by sucrose density gradient centrifugation. Sodium deoxycholate was used to eliminate membrane contamination. Isolated alpha granules from this species have average dimension of 31 nm in width and 65 nm in length....

journal_title：Journal of bacteriology

authors： Chao L,Bowen CC

更新日期：1971-01-01 00:00:00

abstract：:A fusion between the pCloDF13-derived bacteriocin release protein and beta-lactamase was constructed to investigate the subcellular localization and posttranslational modification of the bacteriocin release protein in Escherichia coli. The signal sequence and 25 of the 28 amino acid residues of the mature bacteriocin ...

journal_title：Journal of bacteriology

authors： Luirink J,Watanabe T,Wu HC,Stegehuis F,de Graaf FK,Oudega B

更新日期：1987-05-01 00:00:00

abstract：:Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily...

journal_title：Journal of bacteriology

authors： Benachour A,Muller C,Dabrowski-Coton M,Le Breton Y,Giard JC,Rincé A,Auffray Y,Hartke A

更新日期：2005-02-01 00:00:00

abstract：:Spx is a global regulator that is widespread among the low-G+C-content gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under nonstress conditions, Spx is rapidly degraded by the ClpXP protease...

journal_title：Journal of bacteriology

authors： Engman J,Rogstam A,Frees D,Ingmer H,von Wachenfeldt C

更新日期：2012-03-01 00:00:00