Thermal death of a hydrocarbon bacterium in a nonaqueous fluid.

Abstract:

:A hydrocarbon-utilizing Brevibacterium which grew into the oil phase of an oil-water system was tested for survival at elevated temperature. Cells suspended in oil and cells that had been resuspended in aqueous solution were tested by placing 1-ml samples of the cell suspension in small test tubes immersed in a controlled-temperature water bath. The resultant survival curves in oil consisted of two parts, a flat shoulder obtained in the first half of the heating period, followed by a break indicating rapid die-off. The break in the curves occurred after 50% of the cells were killed. This occurred at exposures of 25, 15, and 8 min for 78, 88.6, and 96.2 C, respectively. The survival curve for 63.5 C in the aqueous solution was a rapid, exponential die-off. The actual increase in survival of the organism in oil is reflected by the length of the shoulder portion. The shoulder occurs only in an oil medium and is increased by decreasing temperature and increasing age of the culture.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Severance MM,LaRock PA

doi

10.1128/JB.116.3.1287-1292.1973

subject

Has Abstract

pub_date

1973-12-01 00:00:00

pages

1287-92

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

116

pub_type

杂志文章
  • "Position effect" on dominance in the D-serine deaminase system of Escherichia coli K-12.

    abstract::A completely stable F32 merodiploid has been isolated, which has the dsd genotype dsdA(+)dsdCx3 /F -dsdA(+)dsdC(+). The stabilization apparently does not result from an alteration in the F, or from its integration in the chromosome, but rather from a chromosomal aberration. The stable strain can exist in two forms, wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.6.1989-1993.1967

    authors: McFall E

    更新日期:1967-12-01 00:00:00

  • Crystal structure of the hyperthermophilic archaeal DNA-binding protein Sso10b2 at a resolution of 1.85 Angstroms.

    abstract::The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.14.4066-4073.2003

    authors: Chou CC,Lin TW,Chen CY,Wang AH

    更新日期:2003-07-01 00:00:00

  • Protein and ribonucleic acid syntheses in heat-damaged and heat-killed Escherichia coli.

    abstract::Protein and ribonucleic acid (RNA) syntheses were measured in both lethally injured and thermally damaged viable cells after heating at lethal temperatures. Immediately after heating, cells were incubated in growth media containing either [14C]leucine or [3H]uracil. The labeled cells were subsequently treated with pen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.2.492-498.1979

    authors: Dean RG,McGroarty EJ

    更新日期:1979-05-01 00:00:00

  • Alternative transcription factor sigma(B) is involved in regulation of biofilm expression in a Staphylococcus aureus mucosal isolate.

    abstract::Osmotic stress was found to induce biofilm formation in a Staphylococcus aureus mucosal isolate. Inactivation of a global regulator of the bacterial stress response, the alternative transcription factor sigma(B), resulted in a biofilm-negative phenotype and loss of salt-induced biofilm production. Complementation of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.23.6824-6826.2000

    authors: Rachid S,Ohlsen K,Wallner U,Hacker J,Hecker M,Ziebuhr W

    更新日期:2000-12-01 00:00:00

  • Genetic analysis of pathway specificity during posttranslational protein translocation across the Escherichia coli plasma membrane.

    abstract::In Escherichia coli, the SecB/SecA branch of the Sec pathway and the twin-arginine translocation (Tat) pathway represent two alternative possibilities for posttranslational translocation of proteins across the cytoplasmic membrane. Maintenance of pathway specificity was analyzed using a model precursor consisting of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.9.2811-2819.2003

    authors: Blaudeck N,Kreutzenbeck P,Freudl R,Sprenger GA

    更新日期:2003-05-01 00:00:00

  • Physiological effects of growth of an Escherichia coli temperature-sensitive dnaZ mutant at nonpermissive temperatures.

    abstract::The physiological effects of incubation at nonpermissive temperatures of Escherichia coli mutants that carry a temperature-sensitive dnaZ allele [dnaZ(Ts)2016] were examined. The temperature at which the dnaZ(Ts) protein becomes inactivated in vivo was investigated by measurements of deoxyribonucleic acid (DNA) synthe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.1.151-158.1977

    authors: Chu H,Malone MM,Haldenwang WG,Walker JR

    更新日期:1977-10-01 00:00:00

  • Genetic evidence that the XylS regulator of the Pseudomonas TOL meta operon controls the Pm promoter through weak DNA-protein interactions.

    abstract::The activation of the Pm promoter of the meta operon of the TOL plasmid of Pseudomonas putida by its cognate XylS activator protein in the presence and absence of benzoate inducers has been examined in specialized Escherichia coli strains carrying Pm-lacZ fusions and the xylS gene in different configurations in which ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.11.3171-3176.1994

    authors: Kessler B,Herrero M,Timmis KN,de Lorenzo V

    更新日期:1994-06-01 00:00:00

  • Growth phase and pH influence peptide signaling for competence development in Streptococcus mutans.

    abstract::The development of competence by the dental caries pathogen Streptococcus mutans is mediated primarily through the alternative sigma factor ComX (SigX), which is under the control of multiple regulatory systems and activates the expression of genes involved in DNA uptake and recombination. Here we report that the indu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00995-13

    authors: Guo Q,Ahn SJ,Kaspar J,Zhou X,Burne RA

    更新日期:2014-01-01 00:00:00

  • Phase variation of Ag43 is independent of the oxidation state of OxyR.

    abstract::OxyR is a DNA binding protein that differentially regulates a cell's response to hydrogen peroxide-mediated oxidative stress. We previously reported that the reduced form of OxyR is sufficient for repression of transcription of agn43 from unmethylated template DNA, which is essential for deoxyadenosine methylase (Dam)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.7.2203-2209.2003

    authors: Wallecha A,Correnti J,Munster V,van der Woude M

    更新日期:2003-04-01 00:00:00

  • Role of uracil-DNA glycosylase in mutation avoidance by Streptococcus pneumoniae.

    abstract::Uracil-DNA glycosylase activity was found in Streptococcus pneumoniae, and the enzyme was partially purified. An ung mutant lacking the activity was obtained by positive selection of cells transformed with a plasmid containing uracil in its DNA. The effects of the ung mutation on mutagenic processes in S. pneumoniae w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.1.283-290.1991

    authors: Chen JD,Lacks SA

    更新日期:1991-01-01 00:00:00

  • Phospholipase D activity of gram-negative bacteria.

    abstract::A phospholipase hydrolyzing cardiolipin to phosphatidic acid and phosphatidyl glycerol was characterized in gram-negative bacteria but was absent in preparations of gram-positive bacteria, Saccharomyces cerevisiae, and rat liver mitochondria. In cell-free extracts of Escherichia coli, Salmonella typhimurium, Proteus v...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1148-1152.1975

    authors: Cole R,Proulx P

    更新日期:1975-12-01 00:00:00

  • Purification and properties of a hydrogenase from Desulfovibrio vulgaris.

    abstract::The soluble hydrogenase of Desulfovibrio vulgaris was purified and some of its properties are described. The molecular weight was determined for the enzyme by sedimentation equilibrium (45,400) and amino acid analysis (44,800). The hydrogenase appears to be a loosely coiled molecule or to have a high axial ratio, whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.1.249-258.1971

    authors: Haschke RH,Campbell LL

    更新日期:1971-01-01 00:00:00

  • Regulation of expression of glutamine synthetase in a symbiotic Nostoc strain associated with Anthoceros punctatus.

    abstract::A characteristic of N2-fixing cyanobacteria in symbiotic associations appears to be release of N2-derived NH4+. The specific activity of the primary ammonium-assimilating enzyme, glutamine synthetase (GS), was found to be three- to fourfold lower in Nostoc sp. strain 7801 grown in symbiotic association with the bryoph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2471-2475.1987

    authors: Joseph CM,Meeks JC

    更新日期:1987-06-01 00:00:00

  • Coexpression of colanic acid and serotype-specific capsular polysaccharides in Escherichia coli strains with group II K antigens.

    abstract::In Escherichia coli K-12, the rcsA and rcsB gene products are positive regulators in expression of the slime polysaccharide colanic acid. We have previously demonstrated the presence of rcsA sequences in E. coli K1 and K5, strains with group II capsular K antigens, and shown that introduction of multicopy rcsA into th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.20.6725-6730.1993

    authors: Keenleyside WJ,Bronner D,Jann K,Jann B,Whitfield C

    更新日期:1993-10-01 00:00:00

  • The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble with RNA polymerase subunits from Escherichia coli and function in basal and activated transcription both in vivo and in vitro.

    abstract::Sinorhizobium meliloti, a gram-negative soil bacterium, forms a nitrogen-fixing symbiotic relationship with members of the legume family. To facilitate our studies of transcription in S. meliloti, we cloned and characterized the gene for the alpha subunit of RNA polymerase (RNAP). S. meliloti rpoA encodes a 336-amino-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.14.3808-3814.2002

    authors: Peck MC,Gaal T,Fisher RF,Gourse RL,Long SR

    更新日期:2002-07-01 00:00:00

  • Purification and characterization of a molybdenum-pterin-binding protein (Mop) in Clostridium pasteurianum W5.

    abstract::A large-scale fractionation scheme purified the major molybdenum(Mo)-binding protein (Mop) from crude extracts of Clostridium pasteurianum, with a 10 and 0.2% yield of Mo and protein, respectively. The apparent molecular weight of the purified molybdoprotein is 5,700, as determined by sodium dodecyl sulfate-polyacryla...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.688-693.1986

    authors: Hinton SM,Merritt B

    更新日期:1986-11-01 00:00:00

  • Mutations in the -10 TATAAT sequence of the gyrA promoter affect both promoter strength and sensitivity to DNA supercoiling.

    abstract::Transcription of the gyrA and gyrB genes, which encode the subunits of DNA gyrase, increases in response to DNA relaxation. Previous studies have shown that a small segment of DNA extending from the -10 consensus hexamer to the start of transcription encodes the sequence determinants for this response. In this study, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.19.5999-6006.1994

    authors: Straney R,Krah R,Menzel R

    更新日期:1994-10-01 00:00:00

  • Mycobacterium smegmatis dnaA region and autonomous replication activity.

    abstract::Two key elements that are thought to be required for replication initiation in eubacteria are the DnaA protein, a trans-acting factor, and the replication origin, a cis-acting element. As a first step in studying the replication initiation process in mycobacteria, we have isolated a 4-kb chromosomal DNA fragment from ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.22.6527-6535.1995

    authors: Rajagopalan M,Qin MH,Nash DR,Madiraju MV

    更新日期:1995-11-01 00:00:00

  • Salmonella typhimurium mutants generally defective in chemotaxis.

    abstract::The mutations of eight chemotaxis-deficient strains of Salmonella typhimurium, including five new mutants in strain LT2, were mapped by P22 transduction in relation to various fla mot deletions in S. abortus-equi. Seven recessive che mutations mapped between motB and flaC: three, all nontumbling, the che region I, adj...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.3.754-765.1976

    authors: Collins AL,Stocker BA

    更新日期:1976-12-01 00:00:00

  • Effects of a mutation that eliminates UDP glucose-pyrophosphorylase on the pathogenicity of Erwinia carotovora subsp. carotovora.

    abstract::A nonpathogenic mutant of Erwinia carotovora obtained by Mu d1 mutagenesis was defective in the ability to utilize several carbon sources. The basis of the mutation was analyzed biochemically and shown to be a defect in the ability to form UDP glucose-pyrophosphorylase. The nonpathogenic phenotype of the mutant was ca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.473-476.1985

    authors: Jayaswal RK,Bressan RA,Handa AK

    更新日期:1985-10-01 00:00:00

  • Identification of an essential gene of Listeria monocytogenes involved in teichoic acid biogenesis.

    abstract::Listeria monocytogenes is a facultative intracellular gram-positive bacterium responsible for severe opportunistic infections in humans and animals. We had previously identified a gene encoding a putative UDP-N-acetylglucosamine 2-epimerase, a precursor of the teichoic acid linkage unit, in the genome of L monocytogen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00771-06

    authors: Dubail I,Bigot A,Lazarevic V,Soldo B,Euphrasie D,Dupuis M,Charbit A

    更新日期:2006-09-01 00:00:00

  • Enhanced acetohydroxy acid synthase III activity in an ilvH mutant of Escherichia coli K-12.

    abstract::The acetohydroxy acid synthase III isozyme, which catalyzes the first common step in the biosynthesis of isoleucine, leucine, and valine in Escherichia coli K-12, is composed of two subunits, the ilvI and ilvH gene products. A missense mutation in ilvH (ilvH612), which reduced the sensitivity of the enzyme to the end ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.11.5197-5199.1988

    authors: Ricca E,Limauro D,Lago CT,de Felice M

    更新日期:1988-11-01 00:00:00

  • RhaU of Rhizobium leguminosarum is a rhamnose mutarotase.

    abstract::Of the nine genes comprising the L-rhamnose operon of Rhizobium leguminosarum, rhaU has not been assigned a function. The construction of a Delta rhaU strain revealed a growth phenotype that was slower than that of the wild-type strain, although the ultimate cell yields were equivalent. The transport of L-rhamnose int...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01120-07

    authors: Richardson JS,Carpena X,Switala J,Perez-Luque R,Donald LJ,Loewen PC,Oresnik IJ

    更新日期:2008-04-01 00:00:00

  • Genome sequence of Herbaspirillum sp. strain GW103, a plant growth-promoting bacterium.

    abstract::Herbaspirillum sp. strain GW103 was isolated from rhizosphere soil of the reed Phragmites australis on reclaimed land. Here we report the 5.05-Mb draft genome sequence of the strain, providing bioinformation about the agronomic benefits of this strain, such as multiple traits relevant to plant root colonization and pl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00806-12

    authors: Lee GW,Lee KJ,Chae JC

    更新日期:2012-08-01 00:00:00

  • Effect of bacteriophage lambda infection on synthesis of groE protein and other Escherichia coli proteins.

    abstract::We used two-dimensional gel electrophoresis to quantitate the changes in rates of synthesis that follow phage lambda infection for 21 Escherichia coli proteins, including groE and dnaK proteins. Although total protein synthesis and the rates of synthesis of most individual E. coli proteins decreased after infection, s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.3.1050-1063.1982

    authors: Drahos DJ,Hendrix RW

    更新日期:1982-03-01 00:00:00

  • Penicillin acyltransferase in Penicillium chrysogenum.

    abstract::Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.5.1502-1508.1967

    authors: Pruess DL,Johnson MJ

    更新日期:1967-11-01 00:00:00

  • Analysis of Halobacterium halobium gas vesicles.

    abstract::Gas vesicles, isolated from lysed Halobacterium halobium cells, gave an amino acid analysis which accounted for 78% of the weight, and the balance was mainly salt and water. One percent of tightly bound d-galactose was found, as well as 2% of phosphate that was not released by treatment which promotes beta-elimination...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.3.1058-1067.1973

    authors: Krantz MJ,Ballou CE

    更新日期:1973-06-01 00:00:00

  • Detection of an A-factor-responsive protein that binds to the upstream activation sequence of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus.

    abstract::DNA-binding assays using mobility shift polyacrylamide gel electrophoresis revealed the presence of a protein that specifically bound to a restriction fragment -288 to -191 bp upstream from the transcriptional start point of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. The binding sit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.9.2652-2661.1993

    authors: Vujaklija D,Horinouchi S,Beppu T

    更新日期:1993-05-01 00:00:00

  • Circular and linear plasmids of Lyme disease spirochetes have extensive homology: characterization of a repeated DNA element.

    abstract::We have cloned three copies of a repeated DNA segment from Borrelia burgdorferi sensu stricto strain B31, present on both circular and linear plasmids of this and other B. burgdorferi sensu lato strains. The DNA sequences are characterized by a highly homologous segment containing two open reading frames (ORFs), ORF-A...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.8.2287-2298.1996

    authors: Zückert WR,Meyer J

    更新日期:1996-04-01 00:00:00

  • Effects of selected inhibitors on electron transport in Neisseria gonorrhoeae.

    abstract::The electron transport system of Neisseria gonorrhoeae was partially characterized by using spectrophotometric, spectroscopic, and oxygen consumption measurements. The effects of selected electron transport inhibitors (amytal, rotenone, 2-heptyl-4-hydroxyquinoline, antimycin A1, and potassium cyanide [KCN]) on electro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.2.537-545.1978

    authors: Kenimer EA,Lapp DF

    更新日期:1978-05-01 00:00:00