Cloning, sequencing, and expression of the gene encoding cyclic 2, 3-diphosphoglycerate synthetase, the key enzyme of cyclic 2, 3-diphosphoglycerate metabolism in Methanothermus fervidus.

Abstract:

:Cyclic 2,3-diphosphoglycerate synthetase (cDPGS) catalyzes the synthesis of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond in 2,3-diphosphoglycerate. cDPG is known to be accumulated to high intracellular concentrations (>300 mM) as a putative thermoadapter in some hyperthermophilic methanogens. For the first time, we have purified active cDPGS from a methanogen, the hyperthermophilic archaeon Methanothermus fervidus, sequenced the coding gene, and expressed it in Escherichia coli. cDPGS purification resulted in enzyme preparations containing two isoforms differing in their electrophoretic mobility under denaturing conditions. Since both polypeptides showed the same N-terminal amino acid sequence and Southern analyses indicate the presence of only one gene coding for cDPGS in M. fervidus, the two polypeptides originate from the same gene but differ by a not yet identified modification. The native cDPGS represents a dimer with an apparent molecular mass of 112 kDa and catalyzes the reversible formation of the intramolecular phosphoanhydride bond at the expense of ATP. The enzyme shows a clear preference for the synthetic reaction: the substrate affinity and the Vmax of the synthetic reaction are a factor of 8 to 10 higher than the corresponding values for the reverse reaction. Comparison with the kinetic properties of the electrophoretically homogeneous, apparently unmodified recombinant enzyme from E. coli revealed a twofold-higher Vmax of the enzyme from M. fervidus in the synthesizing direction.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Matussek K,Moritz P,Brunner N,Eckerskorn C,Hensel R

doi

10.1128/JB.180.22.5997-6004.1998

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

5997-6004

issue

22

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

杂志文章
  • EutR is a direct regulator of genes that contribute to metabolism and virulence in enterohemorrhagic Escherichia coli O157:H7.

    abstract::Ethanolamine (EA) metabolism is a trait associated with enteric pathogens, including enterohemorrhagic Escherichia coli O157:H7 (EHEC). EHEC causes severe bloody diarrhea and hemolytic uremic syndrome. EHEC encodes the ethanolamine utilization (eut) operon that allows EHEC to metabolize EA and gain a competitive advan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00937-13

    authors: Luzader DH,Clark DE,Gonyar LA,Kendall MM

    更新日期:2013-11-01 00:00:00

  • RNA arbitrarily primed PCR survey of genes regulated by ToxR in the deep-sea bacterium Photobacterium profundum strain SS9.

    abstract::We are currently investigating the role of ToxR-mediated gene regulation in Photobacterium profundum strain SS9. SS9 is a moderately piezophilic ("pressure loving") psychrotolerant marine bacterium belonging to the family Vibrionaceae. In Vibrio cholerae, ToxR is a transmembrane DNA binding protein involved in mediati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.5.1688-1693.2001

    authors: Bidle KA,Bartlett DH

    更新日期:2001-03-01 00:00:00

  • NUTRITIONAL MUTATIONS AND TRANSDUCTION BY ULTRAVIOLET-INACTIVATED PHAGE IN STAPHYLOCOCCUS AUREUS.

    abstract::Carere, A., and Isabella Spada-Sermonti (Istituto Superiore di Sanità, Rome, Italy). Nutritional mutations and transduction by ultraviolet-inactivated phage in Staphylococcus aureus. J. Bacteriol. 88:226-232. 1964.-By use of a semisynthetic medium, various nutritional mutants of Staphylococcus aureus 80 were isolated....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.1.226-232.1964

    authors: CARERE A,SPADA-SERMONTI I

    更新日期:1964-07-01 00:00:00

  • EXPLANATION FOR LIMITATION OF POPULATIONS OF ESCHERICHIA COLI IN BROTH CULTURES.

    abstract::Freter, Rolf (Jefferson Medical College, Philadelphia, Pa.), and Atsushi Ozawa. Explanation for limitation of populations of Escherichia coli in broth cultures. J. Bacteriol. 86:904-910. 1963.-Veal Infusion Broth surrounding a cellophane bag containing a 24-hr culture of Escherichia coli was inhibitory to an inoculum ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.5.904-910.1963

    authors: FRETER R,OZAWA A

    更新日期:1963-11-01 00:00:00

  • Characterization of the membrane-bound succinic dehydrogenase of Micrococcus lysodeikticus.

    abstract::The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC 1.3.99.1] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.1.230-238.1971

    authors: Pollock JJ,Linder R,Salton MR

    更新日期:1971-07-01 00:00:00

  • DifA, a methyl-accepting chemoreceptor protein-like sensory protein, uses a novel signaling mechanism to regulate exopolysaccharide production in Myxococcus xanthus.

    abstract::DifA is a methyl-accepting chemotaxis protein (MCP)-like sensory transducer that regulates exopolysaccharide (EPS) production in Myxococcus xanthus. Here mutational analysis and molecular biology were used to probe the signaling mechanisms of DifA in EPS regulation. We first identified the start codon of DifA experime...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00944-10

    authors: Xu Q,Black WP,Nascimi HM,Yang Z

    更新日期:2011-02-01 00:00:00

  • Mapping of a structural gene for valyl-transfer ribonucleic acid synthetase in Escherichia coli by transduction.

    abstract::A structural gene, valS, for the valyl-transfer ribonucleic acid synthetase of Escherichia coli has been mapped on the clockwise side of pyrB and is closely linked to it. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.837-839.1969

    authors: Tingle MA,Neidhardt FC

    更新日期:1969-05-01 00:00:00

  • Thermal death of a hydrocarbon bacterium in a nonaqueous fluid.

    abstract::A hydrocarbon-utilizing Brevibacterium which grew into the oil phase of an oil-water system was tested for survival at elevated temperature. Cells suspended in oil and cells that had been resuspended in aqueous solution were tested by placing 1-ml samples of the cell suspension in small test tubes immersed in a contro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.3.1287-1292.1973

    authors: Severance MM,LaRock PA

    更新日期:1973-12-01 00:00:00

  • The "green" form I ribulose 1,5-bisphosphate carboxylase/oxygenase from the nonsulfur purple bacterium Rhodobacter capsulatus.

    abstract::Form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of the Calvin-Benson-Bassham cycle may be divided into two broad phylogenetic groups, referred to as red-like and green-like, based on deduced large subunit amino acid sequences. Unlike the form I enzyme from the closely related organism Rhodobacter spha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.13.3935-3941.1999

    authors: Horken KM,Tabita FR

    更新日期:1999-07-01 00:00:00

  • Genetic characterization of a novel rhizobial plasmid conjugation system in Rhizobium leguminosarum bv. viciae strain VF39SM.

    abstract::Rhizobium leguminosarum strain VF39SM contains two plasmids that have previously been shown to be self-transmissible by conjugation. One of these plasmids, pRleVF39b, is shown in this study to carry a set of plasmid transfer genes that differs significantly from conjugation systems previously studied in the rhizobia b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01234-12

    authors: Ding H,Yip CB,Hynes MF

    更新日期:2013-01-01 00:00:00

  • Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies.

    abstract::Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Victoria A. Haendiges, and James T. Grace, Jr. Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies. J. Bacteriol. 91:1977-1985. 1966.-Yaba tumor poxvirus synthesis in BSC-1 cell culture was followed s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.5.1977-1985.1966

    authors: Yohn DS,Haendiges VA,Grace JT Jr

    更新日期:1966-05-01 00:00:00

  • A new family of integral membrane proteins involved in transport of aromatic amino acids in Escherichia coli.

    abstract::The nucleotide sequence of tnaB of the tryptophanase operon of Escherichia coli is presented. TnaB is a tryptophan-specific permease that is homologous to Mtr, a second tryptophan-specific permease, and to TyrP, a tyrosine-specific permease. Each member of this family appears to contain 11 membrane-spanning domains. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.10.3231-3234.1991

    authors: Sarsero JP,Wookey PJ,Gollnick P,Yanofsky C,Pittard AJ

    更新日期:1991-05-01 00:00:00

  • Selective mRNA degradation by polynucleotide phosphorylase in cold shock adaptation in Escherichia coli.

    abstract::Upon cold shock, Escherichia coli cell growth transiently stops. During this acclimation phase, specific cold shock proteins (CSPs) are highly induced. At the end of the acclimation phase, their synthesis is reduced to new basal levels, while the non-cold shock protein synthesis is resumed, resulting in cell growth re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.9.2808-2816.2001

    authors: Yamanaka K,Inouye M

    更新日期:2001-05-01 00:00:00

  • Heat sensitivity of Azotobacter vinelandii genetic transformation.

    abstract::Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.1.159-168.1983

    authors: Doran JL,Page WJ

    更新日期:1983-07-01 00:00:00

  • Temperature-sensitive lesions in the Francisella novicida valA gene cloned into an Escherichia coli msbA lpxK mutant affecting deoxycholate resistance and lipopolysaccharide assembly at the restrictive temperature.

    abstract::The valAB locus of Francisella novicida has previously been found to be highly similar at the deduced amino acid level to msbA lpxK of Escherichia coli. Both ValA and MsbA are members of the superfamily of ABC transporters, and they appear to have similar functions. In this study we describe the isolation of a tempera...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.24.7638-7643.1997

    authors: McDonald MK,Cowley SC,Nano FE

    更新日期:1997-12-01 00:00:00

  • Degradation of pyruvate by Micrococcus lactilyticus. III. Properties and cofactor requirements of the carbon dioxide-exchange reaction.

    abstract::Whiteley, H. R. (University of Washington, Seattle) and N. G. McCormick. Degradation of pyruvate by Micrococcus lactilyticus. III. Properties and cofactor requirements of the carbon dioxide-exchange reaction. J. Bacteriol. 85:382-393. 1963.-At an acid pH, extracts of Micrococcus lactilyticus (Veillonella alcalescens) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.2.382-393.1963

    authors: WHITELEY HR,McCORMICK NG

    更新日期:1963-02-01 00:00:00

  • Sialidase-like enzymes produced by group A, B, C, G, and L streptococci and by Streptococcus sanguis.

    abstract::A group of enzymes were prepared from the culture fluids of streptococci belonging to groups A, B, C, G, and L, and from a strain of Streptococcus sanguis. These streptococcal enzymes (designated St-sialidases) released a substance shown to belong to the sialic acid group from the specific substrate BSM-St, a sialomuc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.3.1328-1333.1969

    authors: Hayano S,Tanaka A

    更新日期:1969-03-01 00:00:00

  • Effect of osmolarity on phagocytosis.

    abstract::Sbarra, Anthony J. (St. Margaret's Hospital, Boston, Mass.), William Shirley, and John S. Baumstark. Effect of osmolarity on phagocytosis. J. Bacteriol. 85:306-313. 1963.-The effect of a number of different compounds on phagocytosis was studied. Phagocytosis was monitored by morphological and biochemical means. It was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.2.306-313.1963

    authors: SBARRA AJ,SHIRLEY W,BAUMSTARK JS

    更新日期:1963-02-01 00:00:00

  • Importance of carbon dioxide in the isolation of pneumococci.

    abstract::Austrian, Robert (The University of Pennsylvania School of Medicine, Philadelphia), and Patricia Collins. Importance of carbon dioxide in the isolation of pneumococci. J. Bacteriol. 92:1281-1284. 1966.-Of the strains of pneumococci isolated from man, 8% manifest a requirement for CO(2) if detectable growth is to occur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.5.1281-1284.1966

    authors: Austrian R,Collins P

    更新日期:1966-11-01 00:00:00

  • Osmotic remedial response in a galactose-negative mutant of Saccharomyces cerevisiae.

    abstract::We investigated an osmotic remedial mutant of Saccharomyces which was deficient in galactose-1-phosphate uridyl transferase. Both the rate of growth and the transferase activity of the mutant culture were dependent on the osmotic activity of the growth medium. Organic and ionic solutes proved to be equally effective i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.3.1103-1110.1968

    authors: Bassel J,Douglas HC

    更新日期:1968-03-01 00:00:00

  • Deoxyribonucleic acid characterization of Bdellovibrios.

    abstract::The guanine plus cytosine (GC) content of the deoxyribonucleic acid (DNA) of 11 isolates of host-dependent (H-D) bdellovibrios and 18 host-independent (H-I) derivatives was determined from thermal denaturation curves and buoyant densities in CsCl. The H-D and respective H-I cultures have GC contents which are identica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.786-790.1969

    authors: Seidler RJ,Starr MP,Mandel M

    更新日期:1969-11-01 00:00:00

  • Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili.

    abstract::We describe the identification and characterization of two genes and their gene products responsible for determining receptor binding and pilus length in type 1-piliated Escherichia coli. One gene, pilE, conferred the ability of piliated cells to agglutinate guinea pig erythrocytes. The other gene, pilF, determined pi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.640-645.1987

    authors: Maurer L,Orndorff PE

    更新日期:1987-02-01 00:00:00

  • Characterization of CamH from Methanosarcina thermophila, founding member of a subclass of the {gamma} class of carbonic anhydrases.

    abstract::The homotrimeric enzyme Mt-Cam from Methanosarcina thermophila is the archetype of the gamma class of carbonic anhydrases. A search of databases queried with Mt-Cam revealed that a majority of the homologs comprise a putative subclass (CamH) in which there is major conservation of all of the residues essential for the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01164-09

    authors: Zimmerman SA,Tomb JF,Ferry JG

    更新日期:2010-03-01 00:00:00

  • A cryptic miniplasmid from the hyperthermophilic bacterium Thermotoga sp. strain RQ7.

    abstract::An 846-bp cryptic plasmid has been discovered in the hyperthermophilic bacterium Thermotoga sp. strain RQ7. This is the first plasmid described for an organism from this ancient bacterial lineage and the smallest plasmid described to date for any organism. Nucleotide sequencing revealed a single open reading frame pos...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.9.2759-2762.1994

    authors: Harriott OT,Huber R,Stetter KO,Betts PW,Noll KM

    更新日期:1994-05-01 00:00:00

  • Role of secondary transporters and phosphotransferase systems in glucose transport by Oenococcus oeni.

    abstract::Glucose uptake by the heterofermentative lactic acid bacterium Oenococcus oeni B1 was studied at the physiological and gene expression levels. Glucose- or fructose-grown bacteria catalyzed uptake of [(14)C]glucose over a pH range from pH 4 to 9, with maxima at pHs 5.5 and 7. Uptake occurred in two-step kinetics in a h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06038-11

    authors: Kim OB,Richter H,Zaunmüller T,Graf S,Unden G

    更新日期:2011-12-01 00:00:00

  • Salmonella typhimurium LT2 strains which are r- m+ for all three chromosomally located systems of DNA restriction and modification.

    abstract::We describe the derivation of two strains of Salmonella typhimurium LT2 which are r- m+ for all three of the known chromosomal genes for the restriction and modification of DNA, hsdLT, hsdSA, and hsdSB; the strains were designated LB5000 and LB5010. LB5000 is a smooth derivative sensitive to phage P22; LB5010 is a gal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.1.471-474.1983

    authors: Bullas LR,Ryu JI

    更新日期:1983-10-01 00:00:00

  • Divergence among genes encoding the elongation factor Tu of Yersinia Species.

    abstract::Elongation factor Tu (EF-Tu), encoded by tuf genes, carries aminoacyl-tRNA to the ribosome during protein synthesis. Duplicated tuf genes (tufA and tufB), which are commonly found in enterobacterial species, usually coevolve via gene conversion and are very similar to one another. However, sequence analysis of tuf gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01067-08

    authors: Isabel S,Leblanc E,Boissinot M,Boudreau DK,Grondin M,Picard FJ,Martel EA,Parham NJ,Chain PS,Bader DE,Mulvey MR,Bryden L,Roy PH,Ouellette M,Bergeron MG

    更新日期:2008-11-01 00:00:00

  • Subapical wall synthesis and wall thickening induced by cycloheximide in hyphae of Aspergillus nidulans.

    abstract::Hyphae of Aspergillus nidulans continued to synthesize all the major polysaccharide components of the cell wall when cycloheximide was added to cultures. In the presence of cycloheximide, hyphae did not elongate, but electron microscopy showed that the walls became thicker around the cell. The conclusion that cyclohex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.819-823.1973

    authors: Sternlicht E,Katz D,Rosenberger RF

    更新日期:1973-05-01 00:00:00

  • Protoplast formation and leakage of intramembrane cell components: induction by the competence activator substance of pneumococci.

    abstract::Treatment of pneumococci with activator (a protein that induces bacterial "competence" to absorb deoxyribonucleic acid molecules and undergo genetic transformation) can cause either protoplast formation or leakage of intracellular components to the medium depending on postincubation conditions. The leaked intracellula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.1.344-353.1975

    authors: Seto H,Tomasz A

    更新日期:1975-01-01 00:00:00

  • A distant upstream site involved in the negative regulation of the Escherichia coli ompF gene.

    abstract::The two-component regulatory system, OmpR-EnvZ, of Escherichia coli K-12 regulates the expression of the major outer membrane porin protein, OmpF. OmpR is a DNA-binding protein which acts as both an activator and a repressor to control ompF transcription. In this article, we describe a new OmpR-binding site that is lo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1309-1315.1994

    authors: Huang KJ,Schieberl JL,Igo MM

    更新日期:1994-03-01 00:00:00