Osmotic remedial response in a galactose-negative mutant of Saccharomyces cerevisiae.

Abstract:

:We investigated an osmotic remedial mutant of Saccharomyces which was deficient in galactose-1-phosphate uridyl transferase. Both the rate of growth and the transferase activity of the mutant culture were dependent on the osmotic activity of the growth medium. Organic and ionic solutes proved to be equally effective in inducing the osmotic remedial response. The galactose pathway enzymes were separable by ultracentrifugation, indicating that a stable interenzyme complex was not formed. The results were consistent with the hypothesis that the corrective effect occurs at the tertiary or quaternary level of organization in an environmentally sensitive protein. The possibility that the osmotic remedial response represents an effect of translation is discussed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bassel J,Douglas HC

doi

10.1128/JB.95.3.1103-1110.1968

subject

Has Abstract

pub_date

1968-03-01 00:00:00

pages

1103-10

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

95

pub_type

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