A cryptic promoter in the O(R) region of bacteriophage lambda.

Abstract:

:A cryptic promoter, designated P alpha, initiates transcription within the O(R) region of bacteriophage lambda. Transcription from P alpha proceeds in the direction of the cI repressor gene from sites 46 and 48 bp preceding the PRM transcription start site. P alpha is likely to compete with both PR and PRM for formation of open complexes, since it is only active when PR is mutated and can be suppressed by mutations that increase PRM activity. In addition, transcription initiation at P alpha is blocked by lambda repressor. Kinetic analysis of relative abundance of the products of in vitro transcription indicated that P alpha was approximately 1/3 as strong as PRM. However, a P alpha mutation had little effect on KBkf (the association rate constant) for PRM. These observations can be explained by the finding that open complexes formed at P alpha are relatively unstable (half-life = 20 to 25 min). Dissociation of RNA polymerase from P alpha allows additional open complexes to form at PR or PRM, and thus the apparent strength of P alpha decreases with increasing preincubation times.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Woody ST,Fong RS,Gussin GN

doi

10.1128/jb.175.17.5648-5654.1993

subject

Has Abstract

pub_date

1993-09-01 00:00:00

pages

5648-54

issue

17

eissn

0021-9193

issn

1098-5530

journal_volume

175

pub_type

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