Abstract:
:McFadden, B. A. (Washington State University, Pullman), and H. R. Homann. Characteristics and intermediates of short-term C(14)O(2) incorporation during ribose oxidation by Hydrogenomonas facilis. J. Bacteriol. 89:839-847. 1965.-Ribose-grown cells of Hydrogenomonas facilis, which had been suspended in growth medium and were oxidizing ribose, were exposed to HC(14)O(3) (-) of high specific activity. The uptake was proportional to cell mass. Short-term uptake (less than 2 min) was completely inhibited by 10(-3)m 2,4-dinitrophenol (DNP) or by <4 x 10(-6)mm-chlorocarbonyl cyanide phenylhydrazone, and to the extent of 42% by 5 x 10(-5)m DNP. The following observations were made in kinetic studies (8, 16, 35, 67, 96, and 181 sec) of fixation in the presence of ribose. Glutamate was extensively labeled in periods up to 3 min. It was one of the major early products, containing 30% of the label at 8 sec. The sugar phosphate fraction was not detectably labeled at 8 or 16 sec, but its C(14)-content increased rapidly to 27% at 35 sec and then slowly decreased. Label in phosphoglycerate, phosphoenolpyruvate, and alanine did not appear until 35 sec, and did not exceed about 7, 2, and 3%, respectively, of the total extracted radioactivity. Adenosine triphosphate and adenosine diphosphate were heavily labeled after fixation in a pilot study for 125 sec. Although considerable radioactivity incorporated during the pilot study was intractable by the extraction procedure employed, virtually no C(14) was found in the residue in poly-beta-hydroxybutyric acid. A large number of amino acids and organic acids and some organic phosphates were not detectably labeled in any of the experiments. Omission of ribose greatly diminished incorporation, particularly into glutamate.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
MCFADDEN BA,HOMANN HRdoi
10.1128/JB.89.3.839-847.1965keywords:
subject
Has Abstractpub_date
1965-03-01 00:00:00pages
839-47eissn
0021-9193issn
1098-5530journal_volume
89pub_type
杂志文章abstract::The kinA (spoIIJ) locus contains a single gene which codes for a protein of 69,170 daltons showing strong homology to the transmitter kinases of two component regulatory systems. The purified kinase autophosphorylates in the presence of ATP and mediates the transfer of phosphate to the Spo0A and Spo0F sporulation regu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.6187-6196.1989
更新日期:1989-11-01 00:00:00
abstract::In Escherichia coli, the three branched-chain amino acid activating enzymes appear to be essential for multivalent repression of the isoleucine- and valine-forming enzymes. The results of experiments with a mutant, strain CU18, having an altered threonine deaminase, indicate that free isoleucine and some form of threo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.1.29-39.1974
更新日期:1974-01-01 00:00:00
abstract::ibeA is a virulence factor found in some extraintestinal pathogenic Escherichia coli (ExPEC) strains from the B2 phylogenetic group and particularly in newborn meningitic and avian pathogenic strains. It was shown to be involved in the invasion process of the newborn meningitic strain RS218. In a previous work, we sho...
journal_title:Journal of bacteriology
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doi:10.1128/JB.00089-12
更新日期:2012-09-01 00:00:00
abstract::Rebers, P. A. (Rutgers, The State University, New Brunswick, N. J.), E. Hurwitz, M. Heidelberger, and S. Estrada-Parra. Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI. J. Bacteriol. 83:335-342. 1962.-The type-specific pneumococcal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.335-342.1962
更新日期:1962-02-01 00:00:00
abstract::Polyglutamate is found in various bacteria, but displays different functions depending on the species and their environment. Here, we describe a minimal polyglutamate synthesis system in Bacillus anthracis. In addition to the three genes previously described as sufficient for polyglutamate synthesis, this system inclu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.22.7765-7772.2005
更新日期:2005-11-01 00:00:00
abstract::Woody-Karrer, Pearl (Palo Alto Medical Research Foundation, Palo Alto, Calif.) and Joseph Greenberg. Resistance and cross resistance of Escherichia coli S mutants to the radiomimetic agent nitrofurazone. J. Bacteriol. 85:1208-1216. 1963.-Cross-resistance relationships are described for 73 mutants of Escherichia coli s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.6.1208-1216.1963
更新日期:1963-06-01 00:00:00
abstract::Legionella pneumophila is the causative agent of the severe and potentially fatal pneumonia Legionnaires' disease. L. pneumophila is able to replicate within macrophages and protozoa by establishing a replicative compartment in a process that requires the Icm/Dot type IVB secretion system. The signals and regulatory p...
journal_title:Journal of bacteriology
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doi:10.1128/JB.01578-08
更新日期:2009-04-01 00:00:00
abstract::The small regulatory protein Crl activates sigma(S) (RpoS), the stationary-phase and general stress response sigma factor. Crl has been reported to bind sigma(S) in vitro and to facilitate the formation of RNA polymerase holoenzyme. In Salmonella enterica serovar Typhimurium, Crl is required for the development of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01919-06
更新日期:2007-04-01 00:00:00
abstract::We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intracellular and extra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2796-2801.1988
更新日期:1988-06-01 00:00:00
abstract::A rare group I intron in a cyanobacterial ribonucleotide reductase gene has been characterized. It contains a mobile insertion sequence element not required for RNA splicing. Ribonucleotide reductase genes were found to be hot spots for all three types of self-splicing intervening sequences, including group I and II i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01424-06
更新日期:2007-03-01 00:00:00
abstract::In Escherichia coli, the GlpT transporter, a member of the major facilitator superfamily, moves external glycerol 3-phosphate (G3P) into the cytoplasm in exchange for cytoplasmic phosphate. Study of intact cells showed that both GlpT and HisGlpT, a variant with an N-terminal six-histidine tag, are inhibited (50% inhib...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.13.3863-3870.2003
更新日期:2003-07-01 00:00:00
abstract::In response to DNA damage or the inhibition of normal DNA replication in Escherichia coli, a set of some 20 unlinked operons is induced through the RecA-mediated cleavage of the LexA repressor. We examined the effect of this SOS response on the transposition of Tn5 and determined that the frequency of transposition is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6910-6918.1991
更新日期:1991-11-01 00:00:00
abstract::The genes for protein synthesis elongation factors Tu and G were cloned from the cyanobacterium Anacystis nidulans. The locations of these genes were mapped within the cloned DNA fragment by hybridization with Escherichia coli probes. The organization of the cloned fragment and the DNA flanking it in the A. nidulans c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.78-82.1986
更新日期:1986-04-01 00:00:00
abstract::Pseudomonas sp. strain ACP is capable of growth on 1-aminocyclopropane-1-carboxylate (ACC) as a nitrogen source owing to induction of the enzyme ACC deaminase and the subsequent conversion of ACC to alpha-ketobutyrate and ammonia (M. Honma, Agric. Biol. Chem. 49:567-571, 1985). The complete amino acid sequence of puri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5260-5265.1991
更新日期:1991-09-01 00:00:00
abstract::The pckA gene of Rhizobium meliloti, encoding phosphoenolpyruvate carboxykinase, was isolated from a genomic cosmid library by complementation of the succinate growth phenotype of a Pck- mutant. The gene region was mapped by subcloning and Tn5 insertion mutagenesis. The DNA sequence for a 2-kb region containing the st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.6.1452-1460.1995
更新日期:1995-03-01 00:00:00
abstract::Containment strategies for outbreaks of invasive Neisseria meningitidis disease are informed by serogroup assays that characterize the polysaccharide capsule. We sought to uncover the genomic basis of conflicting serogroup assay results for an isolate (M16917) from a patient with acute meningococcal disease. To this e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06604-11
更新日期:2012-10-01 00:00:00
abstract::We report the complete genome sequence of Helicobacter cinaedi strain PAGU611, isolated in a case of human bacteremia. The PAGU611 genome comprises a 2,078,348-bp chromosome and a 23,054-bp plasmid. The chromosome contains a unique genomic island, encoding a type VI secretion system and clustered regularly interspaced...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00645-12
更新日期:2012-07-01 00:00:00
abstract::Brucella native haptens (NHs) extracted with hot water from smooth (S)-type B. abortus and B. melitensis were purified to high levels of serological activity and compared with the polysaccharide obtained by acid hydrolysis (PS) of the S lipopolysaccharide (S-LPS). By 13C nuclear magnetic resonance analysis, NHs showed...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.4.1070-1079.1996
更新日期:1996-02-01 00:00:00
abstract::Manohar, M. (University of Minnesota, St. Paul), S. Kumar, and R. K. Lindorfer. Heat reactivation of the alpha-hemolytic, dermonecrotic, and lethal activities of crude and purified staphylococcal alpha-toxin. J. Bacteriol. 91:1681-1685. 1966.-Crude staphylococcal toxin loses its alpha-hemolytic activity more rapidly a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1681-1685.1966
更新日期:1966-05-01 00:00:00
abstract::Many gram-negative bacteria harbor a copper/zinc-containing superoxide dismutase (CuZnSOD) in their periplasms. In pathogenic bacteria, one role of this enzyme may be to protect periplasmic biomolecules from superoxide that is released by host phagocytic cells. However, the enzyme is also present in many nonpathogens ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00554-06
更新日期:2006-09-01 00:00:00
abstract::The intracellular levels of cyclic adenosine 3',5'-monophosphate (cyclic AMP) were measured at various intervals during growth and morphogenesis in Arthrobacter crystallopoietes. Cyclic AMP levels remained relatively constant throughout growth in spherical cells grown in glucose-based media. Immediately after inoculat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.874-879.1977
更新日期:1977-02-01 00:00:00
abstract::A previously undetected Bacillus subtilis aspartokinase isozyme, which we have called aspartokinase III, has been characterized. The new isozyme was most readily detected in extracts of cells grown with lysine, which repressed aspartokinase II and induced aspartokinase III, or in extracts of strain VS11, a mutant lack...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.218-223.1990
更新日期:1990-01-01 00:00:00
abstract::In Zymomonas mobilis, three- to fourfold more glyceraldehyde-3-phosphate dehydrogenase protein than phosphoglycerate kinase is needed for glycolysis because of differences in catalytic efficiency. Consistent with this requirement, higher levels of glyceraldehyde-3-phosphate dehydrogenase were observed with two-dimensi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.245-254.1991
更新日期:1991-01-01 00:00:00
abstract::The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.5.1364-1373.2000
更新日期:2000-03-01 00:00:00
abstract::The transduction efficiency of Tn551, a staphylococcal transposon coding for erythromycin resistance (ermB), was increased by a factor of about five in acceptor strains carrying the gene for methicillin resistance (mec), as compared with methicillin-sensitive strains. This enhancement was independent of the generalize...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.533-535.1983
更新日期:1983-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6109-6116.1992
更新日期:1992-10-01 00:00:00
abstract::The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4544-4548.1995
更新日期:1995-08-01 00:00:00
abstract::Yersinia pestis is an important human pathogen that is maintained in flea-rodent enzootic cycles in many parts of the world. During its life cycle, Y. pestis senses host-specific environmental cues such as temperature and regulates gene expression appropriately to adapt to the insect or mammalian host. For example, Y....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.4.1381-1388.2006
更新日期:2006-02-01 00:00:00
abstract::Erwinia chrysanthemi 3937 secretes several pectinolytic enzymes, among which eight isoenzymes of pectate lyases with an endo-cleaving mode (PelA, PelB, PelC, PelD, PelE, PelI, PelL, and PelZ) have been identified. Two exo-cleaving enzymes, the exopolygalacturonate lyase, PelX, and an exo-poly-alpha-D-galacturonosidase...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.5.1652-1663.1999
更新日期:1999-03-01 00:00:00
abstract::The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC 1.3.99.1] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.1.230-238.1971
更新日期:1971-07-01 00:00:00