Abstract:
:Manohar, M. (University of Minnesota, St. Paul), S. Kumar, and R. K. Lindorfer. Heat reactivation of the alpha-hemolytic, dermonecrotic, and lethal activities of crude and purified staphylococcal alpha-toxin. J. Bacteriol. 91:1681-1685. 1966.-Crude staphylococcal toxin loses its alpha-hemolytic activity more rapidly at 60 than at 100 C. This paradoxical behavior has been postulated to be due to the presence of a thermolabile inhibitor in crude toxin. This work provides experimental evidence for the presence of a thermolabile "protective inhibitor." This substance(s) protects the alpha-toxin against destruction at 60 C, yet simultaneously inhibits the hemolytic activity of alpha-toxin under the same conditions. Of greater importance, this work also demonstrates that the dermonecrotic and lethal activities of crude toxin are inactivated and reactivated in parallel with the alpha-hemolytic activity. Crude staphylococcal toxin possessing a high alpha-hemolytic titer when heated to 60 C for 30 min lost its alpha-hemolytic, dermonecrotic, and lethal activity. However, when this same toxin was immediately exposed to 100 C, a remarkable simultaneous reactivation of all three of these activities occurred. Contrariwise, electrophoretically purified alpha-hemolysin, which also possessed dermonecrotic and lethal activity, showed no reactivation under these conditions, thus demonstrating that reactivation is due to a substance(s) distinct from the alpha-toxin. The fact that alpha-hemolytic, dermonecrotic, and lethal activities were inactivated at 60 C and simultaneously reactivated at 100 C provides additional proof that these activities are all associated with one toxic component. The probability is remote that three separate entities would exhibit the same rate of inactivation and the same strange reactivation.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Manohar M,Kumar S,Lindorfer RKdoi
10.1128/JB.91.5.1681-1685.1966subject
Has Abstractpub_date
1966-05-01 00:00:00pages
1681-5issue
5eissn
0021-9193issn
1098-5530journal_volume
91pub_type
杂志文章abstract::Pyruvate kinase of Streptococcus mutans JC 2 had an absolute and specific requirement for glucose-6-phosphate. Inorganic phosphate was a strong inhibitor. The enzyme required K+ or NH4+ and Mg2+ or Mn2+. S. mutans FIL and E 49, Streptococcus bovis ATCC 9809, and Streptococcus salivarius ATCC 13419 had also glucose-6-p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.562-563.1975
更新日期:1975-10-01 00:00:00
abstract::Twelve strains of Agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, Kalanchoe, and carrot. Eleven strains contained plasmids of molecular weights 77 X 10(6) to 182 X 10(6) as determined by electron microscopy. One strain contained only a sm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.76-80.1977
更新日期:1977-01-01 00:00:00
abstract::Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.13.4052-4060.2001
更新日期:2001-07-01 00:00:00
abstract::The Bacillus subtilis sacY and sacT genes encode antiterminator proteins, similar to the Escherichia coli bglG gene product and required for transcription of sucrose metabolism genes. A Tn10 insertion into bglP (formerly sytA) has been previously identified as restoring sucrose utilization to a strain with deletions o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.6.1527-1535.1995
更新日期:1995-03-01 00:00:00
abstract::Hyatt, Mildred T. (Pioneering Research Division. U.S. Army Natick Laboratories, Natick, Mass.), and Hillel S. Levinson. Effect of sugars and other carbon compounds on germination and postgerminative development of Bacillus megaterium spores. J. Bacteriol. 88:1403-1415. 1964.-A total of 77 carbon-containing compounds w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.5.1403-1415.1964
更新日期:1964-11-01 00:00:00
abstract::Structural studies were carried out on lipoteichoic acids obtained from defatted cells of 10 Bacillus strains by phenol-water partition followed by chromatography on DEAE-Sephacel and Octyl-Sepharose columns. A group of the tested bacteria (group A), Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus, was...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.2.508-516.1986
更新日期:1986-08-01 00:00:00
abstract::Succinyl-coenzyme A synthetase of Escherichia coli has an alpha 2 beta 2 subunit structure. By measuring reconstituted enzyme activity present after addition of purified alpha or beta subunits to cell extracts followed by refolding, we have shown that extracts contain no significant excess of either subunit species. T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.231-237.1980
更新日期:1980-07-01 00:00:00
abstract::Fruiting body formation in Myxococcus xanthus involves the aggregation of cells to form mounds and the differentiation of rod-shaped cells into spherical myxospores. The surface of the myxospore is composed of several sodium dodecyl sulfate (SDS)-soluble proteins, the best characterized of which is protein S (Mr, 19,0...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.2141-2145.1991
更新日期:1991-03-01 00:00:00
abstract::Promoters were cloned from the chromosomal DNA of Lactococcus lactis subsp. lactis NP4510 by using promoter-probe vector pGKV210. N-Methyl-N'-nitro-N-nitrosoguanidine-induced mutagenesis of L. lactis-(pBV413), with low-level expression of the cat-86 gene, resulted in enhanced expression. Subcloning and sequencing of t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.21.6754-6758.1994
更新日期:1994-11-01 00:00:00
abstract::Biphenyl metabolism in Aspergillus toxicarius occurs by successive hydroxylations in the 4- and 4'-positions, followed by conjugation with sulfate to produce 4-hydroxybiphenyl-O-sulfonic acid and 4,4'-dihydroxybiphenyl-O-sulfonic acid. The hydroxylation reactions normally occur only after a prolonged lag period after ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.49-57.1983
更新日期:1983-10-01 00:00:00
abstract::Cecropins are a family of small, basic antibacterial polypeptides which can be isolated from pupae of immunized Lepidoptera. They are active against both gram-negative and gram-positive bacteria. We studied a mutant of Escherichia coli, strain SB1004, which is more sensitive to cecropin D than is the parental strain. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.170-176.1983
更新日期:1983-04-01 00:00:00
abstract::Acidified phenol extracts prepared from competent cultures of a group H Streptococcus strain Wicky made competent with competence factor derived from cultures of another group H Streptococcus, strain Challis, showed a difference in polyacrylamide-gel protein patterns when compared to extracts prepared from noncompeten...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.360-362.1970
更新日期:1970-10-01 00:00:00
abstract::Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunological techniques, we have compared the synthesis of the phoA protein (alkaline phosphatase) and the phoS protein (phosphate-binding protein) in response to the level of phosphate in the medium in different genetic backgrounds containing the kn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.595-609.1976
更新日期:1976-07-01 00:00:00
abstract::We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion of the target gene i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.636-643.1984
更新日期:1984-05-01 00:00:00
abstract::Pasteurella multocida is the causative agent of a range of diseases with economic importance in production animals. Many systems have been employed to identify virulence factors of P. multocida, including in vivo expression technology (IVET), signature-tagged mutagenesis, and whole-genome expression profiling. In a pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.7.2278-2285.2005
更新日期:2005-04-01 00:00:00
abstract::Mycolic acids are a key component of the mycobacterial cell wall, providing structure and forming a major permeability barrier. In Mycobacterium tuberculosis mycolic acids are synthesized by type I and type II fatty acid synthases. One of the enzymes of the type II system is encoded by fabG1. We demonstrate here that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01740-06
更新日期:2007-05-01 00:00:00
abstract::Escherichia coli exhibits a wide range of lifestyles encompassing commensalism and various pathogenic behaviors which its highly dynamic genome contributes to develop. How environmental and host factors shape the genetic structure of E. coli strains remains, however, largely unknown. Following a previous study of E. c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01192-10
更新日期:2011-03-01 00:00:00
abstract::Bishop, D. G. (University of Sydney, Sydney, Australia), and J. L. Still. Fatty acid metabolism in Serratia marcescens. J. Bacteriol. 82:370-375. 1961.-A study has been made of the oxidation of saturated fatty acids containing between two and 18 carbon atoms by whole cells of the bacterium, Serratia marcescens. This o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.3.370-375.1961
更新日期:1961-09-01 00:00:00
abstract::Glycine betaine is an effective osmoprotectant for Bacillus subtilis. Its import into osmotically stressed cells led to the buildup of large pools, whose size was sensitively determined by the degree of the osmotic stress imposed. The amassing of glycine betaine caused repression of the formation of an osmostress-adap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01505-12
更新日期:2013-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.13.4098-4102.1999
更新日期:1999-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.188.4.1286-1294.2006
更新日期:2006-02-01 00:00:00
abstract::The chpB locus is a chromosomal homolog of the pem locus, which is responsible for stable maintenance of plasmid R100 within the host cells. Like pem, chpB codes for two genes, chpBK and chpBI, encoding a growth inhibitor and a suppressor for the killing action of the ChpBK protein, respectively. Here, we determined t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5861-5863.1994
更新日期:1994-09-01 00:00:00
abstract::To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.1.280-283.1997
更新日期:1997-01-01 00:00:00
abstract::A systematic analysis of the type III secretion (T3S) genes of Pseudomonas aeruginosa strain PAO1 revealed that they are under quorum-sensing control. This observation was supported by the down-regulation of the T3S regulon in the presence of RhlR-C4HSL and the corresponding advanced secretion of ExoS in a rhlI mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.11.3898-3902.2005
更新日期:2005-06-01 00:00:00
abstract::HlyD, a member of the membrane fusion protein family, is essential for the secretion of the RTX hemolytic toxin HlyA from Escherichia coli. Random point mutations affecting HlyA secretion were obtained, distributed in most periplasmic regions of the HlyD molecule. Analysis of the secretion phenotypes of different muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.21.7471-7480.2005
更新日期:2005-11-01 00:00:00
abstract::Biberstein, Ernst L. (University of California, Davis) and Patricia D. Spencer. Oxidative metabolism of Haemophilus species grown at different levels of hemin supplementation. J. Bacteriol. 84:916-920. 1962.-Haemophilus strains were propagated on hemin-deficient and hemin-supplemented media and tested on various subst...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.5.916-920.1962
更新日期:1962-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.786-792.1980
更新日期:1980-02-01 00:00:00
abstract::A 22-kb segment of chromosomal DNA from Enterococcus faecalis OG1RF containing the pyrimidine biosynthesis genes pyrC and pyrD was previously detected as complementing Escherichia coli pyrC and pyrD mutations. In the present study, it was found that the E. faecalis pyrimidine biosynthetic genes in this clone (designat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.23.6866-6873.1995
更新日期:1995-12-01 00:00:00
abstract::The colH gene encoding a collagenase was cloned from Clostridium histolyticum JCM 1403. Nucleotide sequencing showed a major open reading frame encoding a 116-kDa protein of 1,021 amino acid residues. The deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, HEX...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.21.6489-6496.1994
更新日期:1994-11-01 00:00:00
abstract::During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.18.6198-6207.2004
更新日期:2004-09-01 00:00:00