Abstract:
:Glycine betaine is an effective osmoprotectant for Bacillus subtilis. Its import into osmotically stressed cells led to the buildup of large pools, whose size was sensitively determined by the degree of the osmotic stress imposed. The amassing of glycine betaine caused repression of the formation of an osmostress-adaptive pool of proline, the only osmoprotectant that B. subtilis can synthesize de novo. The ABC transporter OpuA is the main glycine betaine uptake system of B. subtilis. Expression of opuA was upregulated in response to both sudden and sustained increases in the external osmolarity. Nonionic osmolytes exerted a stronger inducing effect on transcription than ionic osmolytes, and this was reflected in the development of corresponding OpuA-mediated glycine betaine pools. Primer extension analysis and site-directed mutagenesis pinpointed the osmotically controlled opuA promoter. Deviations from the consensus sequence of SigA-type promoters serve to keep the transcriptional activity of the opuA promoter low in the absence of osmotic stress. opuA expression was downregulated in a finely tuned manner in response to increases in the intracellular glycine betaine pool, regardless of whether this osmoprotectant was imported or was newly synthesized from choline. Such an effect was also exerted by carnitine, an effective osmoprotectant for B. subtilis that is not a substrate for the OpuA transporter. opuA expression was upregulated in a B. subtilis mutant that was unable to synthesize proline in response to osmotic stress. Collectively, our data suggest that the intracellular solute pool is a key determinant for the osmotic control of opuA expression.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hoffmann T,Wensing A,Brosius M,Steil L,Völker U,Bremer Edoi
10.1128/JB.01505-12subject
Has Abstractpub_date
2013-02-01 00:00:00pages
510-22issue
3eissn
0021-9193issn
1098-5530pii
JB.01505-12journal_volume
195pub_type
杂志文章abstract::A tandem pair of nearly identical genes from Serpulina hyodysenteriae (B204) were cloned and sequenced. The full open reading frame of one gene and the partial open reading frame of the neighboring gene appear to encode secreted proteins which are homologous to, yet distinct from, the 39-kDa extracytoplasmic protein p...
journal_title:Journal of bacteriology
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doi:10.1128/JB.180.2.444-448.1998
更新日期:1998-01-01 00:00:00
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doi:10.1128/jb.179.8.2534-2539.1997
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doi:10.1128/JB.93.6.1794-1799.1967
更新日期:1967-06-01 00:00:00
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doi:10.1128/JB.135.1.289-290.1978
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doi:10.1128/jb.174.9.2898-2902.1992
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.221-227.1976
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1971-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.139-146.1974
更新日期:1974-10-01 00:00:00
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doi:10.1128/jb.178.3.611-618.1996
更新日期:1996-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.1.654-656.1981
更新日期:1981-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.4.574-577.1961
更新日期:1961-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.4783-4789.1990
更新日期:1990-09-01 00:00:00
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doi:10.1128/JB.134.2.476-482.1978
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更新日期:2003-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1965-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-05-09 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2003-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.1.1-5.1969
更新日期:1969-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.2.781-784.1984
更新日期:1984-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.6.2028-2031.1992
更新日期:1992-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.1208-1210.1984
更新日期:1984-06-01 00:00:00
abstract::Transcription-coupled repair (TCR) is a cellular process by which some forms of DNA damage are repaired more rapidly from transcribed strands of active genes than from nontranscribed strands or the overall genome. In humans, the TCR coupling factor, CSB, plays a critical role in restoring transcription following both ...
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pub_type: 杂志文章
doi:10.1128/JB.06725-11
更新日期:2012-05-01 00:00:00