Escherichia coli tol-pal mutants form outer membrane vesicles.

Abstract:

:Mutations in the tol-pal genes induce pleiotropic effects such as release of periplasmic proteins into the extracellular medium and hypersensitivity to drugs and detergents. Other outer membrane defective strains such as tolC, lpp, and rfa mutations are also altered in their outer membrane permeability. In this study, electron microscopy and Western blot analyses were used to show that strains with mutations in each of the tol-pal genes formed outer membrane vesicles after growth in standard liquid or solid media. This phenotype was not observed in tolC and rfaD cells in the same conditions. A tolA deletion in three different Escherichia coli strains was shown to lead to elevated amounts of vesicles. These results, together with plasmid complementation experiments, indicated that the formation of vesicles resulted from the defect of any of the Tol-Pal proteins. The vesicles contained outer membrane trimeric porins correctly exposed at the cell surface. Pal outer membrane lipoprotein was also immunodetected in the vesicle fraction of tol strains. The results are discussed in view of the role of the Tol-Pal transenvelope proteins in maintaining outer membrane integrity by contributing to target or integrate newly synthesized components of this structure.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bernadac A,Gavioli M,Lazzaroni JC,Raina S,Lloubès R

doi

10.1128/JB.180.18.4872-4878.1998

subject

Has Abstract

pub_date

1998-09-01 00:00:00

pages

4872-8

issue

18

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

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