Insertional inactivation of genes encoding components of the sodium-type flagellar motor and switch of Vibrio parahaemolyticus.

abstract：:The Aer protein in Escherichia coli is a membrane-bound, FAD-containing aerotaxis and energy sensor that putatively monitors the redox state of the electron transport system. Binding of FAD to Aer requires the N-terminal PAS domain and residues in the F1 region and C-terminal HAMP domain. The PAS domains of other PAS ...

journal_title：Journal of bacteriology

authors： Herrmann S,Ma Q,Johnson MS,Repik AV,Taylor BL

更新日期：2004-10-01 00:00:00

abstract：:A three-dimensional tracking microscope was used to quantify the effects of temperature (50 to 80 degrees C) and pH (2 to 4) on the motility of Sulfolobus acidocaldarius, a thermoacidophilic archaeon. Swimming speed and run time increased with temperature but remained relatively unchanged with increasing pH. These res...

journal_title：Journal of bacteriology

authors： Lewus P,Ford RM

更新日期：1999-07-01 00:00:00

abstract：:trpG of Bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and L-tryptophan. Expression of trpG is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. Examination of both transcriptional and transl...

journal_title：Journal of bacteriology

authors： Yang M,de Saizieu A,van Loon AP,Gollnick P

更新日期：1995-08-01 00:00:00

abstract：:The ftsH gene encodes an ATP- and Zn(2+)-dependent metalloprotease which is anchored to the cytoplasmic membrane via two transmembrane segments in such a way that the very short amino- and the long carboxy termini are exposed to the cytoplasm. Deletion of the ftsH gene in Bacillus subtilis results in a pleiotropic phe...

journal_title：Journal of bacteriology

authors： Wehrl W,Niederweis M,Schumann W

更新日期：2000-07-01 00:00:00

abstract：:A heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of Pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. The original bacterial culture had been obtained from a bison that died of hemorrhagi...

journal_title：Journal of bacteriology

authors： Rebers PA,Heddleston KL,Rhoades KR

更新日期：1967-01-01 00:00:00

abstract：:An alpha-L-arabinofuranosidase (EC 3.2.1.55) from the culture fluid of Rhodotorula flava IFO 0407 grown on beet arabinan as a carbon source has been highly purified. The purified enzyme has a pH optimum of 2.0. The enzyme is unusually acid stable, retaining 82% of its activity after being maintained for 24 h at pH 1.5...

journal_title：Journal of bacteriology

authors： Uesaka E,Sato M,Raiju M,Kaji A

更新日期：1978-03-01 00:00:00

abstract：:The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded within the SPI-2 locus, which sh...

journal_title：Journal of bacteriology

authors： Miki T,Shibagaki Y,Danbara H,Okada N

更新日期：2009-11-01 00:00:00

abstract：:Proteins induced by acid or base, during long-term aerobic or anaerobic growth in complex medium, were identified in Escherichia coli. Two-dimensional gel electrophoresis revealed pH-dependent induction of 18 proteins, nine of which were identified by N-terminal sequencing. At pH 9, tryptophan deaminase (TnaA) was ind...

journal_title：Journal of bacteriology

authors： Blankenhorn D,Phillips J,Slonczewski JL

更新日期：1999-04-01 00:00:00

abstract：:The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the r...

journal_title：Journal of bacteriology

authors： Hackstadt T,Todd WJ,Caldwell HD

更新日期：1985-01-01 00:00:00

abstract：:The nucleotide sequence of a 7.3-kilobase-pair fragment of DNA encoding a hemolytic activity from Serratia marcescens was determined. Two large open reading frames were identified, designated shlA (Serratia hemolysin) and shlB, capable of encoding polypeptides of 165, 056 and 61,897 molecular weight, respectively. Bot...

journal_title：Journal of bacteriology

authors： Poole K,Schiebel E,Braun V

更新日期：1988-07-01 00:00:00

abstract：:Green fluorescent protein (GFP) fusions, immunofluorescence microscopy, and cryo-electron tomography revealed that the chemoreceptors of the Lyme disease spirochete Borrelia burgdorferi form long, thin arrays near both cell poles. These arrays are in close proximity to the flagellar motors. This information provides a...

journal_title：Journal of bacteriology

authors： Xu H,Raddi G,Liu J,Charon NW,Li C

更新日期：2011-05-01 00:00:00

abstract：:Crude cell extracts of Bacillus subtilis 168T exhibit enzyme activity capable of releasing free uracil from phage PBS1 deoxyribonucleic acid (DNA) in the presence of ethylenediaminetetraacetate. By measuring the enzyme activity in 300 clones that emanated from mutagenized cells, we obtained a mutant strain that did no...

journal_title：Journal of bacteriology

authors：

更新日期：1977-08-01 00:00:00

abstract：:The regulatory properties of acetohydroxy acid synthetase (AHAS), the first enzyme in the biosynthetic pathway to valine and the second in the isoleucine pathway, were investigated in the fission yeast Schizosaccharomyces pombe. The enzyme was partially purified from crude extracts by protamine sulfate treatment, ammo...

journal_title：Journal of bacteriology

authors： McDonald RA,Satyanarayana T,Kaplan JG

更新日期：1973-04-01 00:00:00

abstract：:Chlamydial lipopolysaccharide (LPS) agglutinated mouse and rabbit erythrocytes but not human, guinea pig, or pronghorn antelope erythrocytes. Hemagglutination was not specific for Chlamydia spp., as rough LPSs from Coxiella burnetii and Escherichia coli also agglutinated erythrocytes from the same animal species. Nona...

journal_title：Journal of bacteriology

authors： Watkins NG,Caldwell HD,Hackstadt T

更新日期：1987-08-01 00:00:00

abstract：:Deletion of sigE, the structural gene for the sporulation-induced RNA polymerase sigma factor, sigma E, prevented endospore formation by Bacillus subtilis. The effects of integration of plasmids into the sigE region of the chromosome and the use of complementation analyses demonstrated that sigE is part of an operon t...

journal_title：Journal of bacteriology

authors： Kenney TJ,Moran CP Jr

更新日期：1987-07-01 00:00:00

abstract：:Comamonas sp. strain CNB-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. Deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. In this study, a novel deaminase wa...

journal_title：Journal of bacteriology

authors： Liu L,Wu JF,Ma YF,Wang SY,Zhao GP,Liu SJ

更新日期：2007-04-01 00:00:00

abstract：:Bacillus subtilis grows under anaerobic conditions utilizing nitrate ammonification and various fermentative processes. The two-component regulatory system ResDE and the redox regulator Fnr are the currently known parts of the regulatory system for anaerobic adaptation. Mutation of the open reading frame ywiD located ...

journal_title：Journal of bacteriology

authors： Marino M,Ramos HC,Hoffmann T,Glaser P,Jahn D

更新日期：2001-12-01 00:00:00

abstract：:The neuS-encoded polysialytransferase (polyST) in Escherichia coli K1 catalyzes synthesis of polysialic acid homopolymers composed of unbranched sialyl alpha 2,8 linkages. Subcloning and complementation experiments showed that the K1 neuS was functionally interchangeable with the neuS from E. coli K92 (S. M. Steenberg...

journal_title：Journal of bacteriology

authors： Vimr ER,Bergstrom R,Steenbergen SM,Boulnois G,Roberts I

更新日期：1992-08-01 00:00:00

abstract：:Haight, Rodger D. (Oregon State University, Corvallis), and Richard Y. Morita. Thermally induced leakage from Vibrio marinus, an obligately psychrophilic bacterium. J. Bacteriol. 92:1388-1393. 1966.-Leakage of various cellular components into the surrounding menstruum occurred when Vibrio marinus was subjected to temp...

journal_title：Journal of bacteriology

authors： Haight RD,Morita RY

更新日期：1966-11-01 00:00:00

abstract：:The ugp operon of Escherichia coli includes genes involved in the uptake of sn-glycerol-3-phosphate and glycerophosphoryl diesters and belongs to the pho regulon which is induced by phosphate limitation. This operon has two transcriptional initiation sites, as determined by S1 nuclease mapping of the in vivo transcrip...

journal_title：Journal of bacteriology

authors： Kasahara M,Makino K,Amemura M,Nakata A,Shinagawa H

更新日期：1991-01-01 00:00:00

abstract：:To isolate strains with new recA mutations that differentially affect RecA protein functions, we mutagenized in vitro the recA gene carried by plasmid mini-F and then introduced the mini-F-recA plasmid into a delta recA host that was lysogenic for prophage phi 80 and carried a lac duplication. By scoring prophage indu...

journal_title：Journal of bacteriology

authors： Dutreix M,Moreau PL,Bailone A,Galibert F,Battista JR,Walker GC,Devoret R

更新日期：1989-05-01 00:00:00

abstract：:A novel of Escherichia coli endopeptidase was used for a selective partial hydrolysis of the peptide bridges which interlink the glycan chains in E. coli sacculi. The loosening of the murein network revealed, in the electron microscope, a preferential orientation of the glycan chains, more or less perpendicular to the...

journal_title：Journal of bacteriology

authors： Verwer RW,Nanninga N,Keck W,Schwarz U

更新日期：1978-11-01 00:00:00

abstract：:In Escherichia coli, putrescine is metabolized to succinate for use as a carbon and nitrogen source by the putrescine utilization pathway (Puu pathway). One gene in the puu gene cluster encodes a transcription factor, PuuR, which has a helix-turn-helix DNA-binding motif. DNA microarray analysis of an E. coli puuR muta...

journal_title：Journal of bacteriology

authors： Nemoto N,Kurihara S,Kitahara Y,Asada K,Kato K,Suzuki H

更新日期：2012-07-01 00:00:00

abstract：:The E. coli RelA protein is a ribosome-dependent (p)ppGpp synthetase that is activated in response to amino acid starvation. RelA can be dissected both functionally and physically into two domains: The N-terminal domain (NTD) (amino acids [aa] 1 to 455) contains the catalytic domain of RelA, and the C-terminal domain ...

journal_title：Journal of bacteriology

authors： Gropp M,Strausz Y,Gross M,Glaser G

更新日期：2001-01-01 00:00:00

abstract：:In Cyanophora paradoxa, the allophycocyanin apoprotein subunits, alpha and beta, are encoded in the cyanelle (plastid) genome. These genes were transferred to the cyanobacterium Synechococcus sp. PCC 7002 on a plasmid replicon. Phycobilisomes isolated from transformed cyanobacteria were found to contain C. paradoxa al...

journal_title：Journal of bacteriology

authors： de Lorimier R,Guglielmi G,Bryant DA,Stevens SE Jr

更新日期：1987-05-01 00:00:00

abstract：:Antiserum to Aeromonas hydrophila A6 cell envelopes was shown in a previous study (C. Y. F. Wong, G. Mayrhofer, M. W. Heuzenroeder, H. M. Atkinson, D. M. Quinn, and R. L. P. Flower, FEMS Immunol. Med. Microbiol. 15:233-241, 1996) to protect mice against lethal infection by this organism. In this study, colony blot ana...

journal_title：Journal of bacteriology

authors： Wong CY,Heuzenroeder MW,Quinn DM,Flower RL

更新日期：1997-06-01 00:00:00

abstract：:Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present the complete genome sequence of R. typhi (1,111,496 bp) and compare it to the two published rickettsial genome sequences: R. prowazekii and ...

journal_title：Journal of bacteriology

authors： McLeod MP,Qin X,Karpathy SE,Gioia J,Highlander SK,Fox GE,McNeill TZ,Jiang H,Muzny D,Jacob LS,Hawes AC,Sodergren E,Gill R,Hume J,Morgan M,Fan G,Amin AG,Gibbs RA,Hong C,Yu XJ,Walker DH,Weinstock GM

更新日期：2004-09-01 00:00:00

abstract：:The Salmonella typhimurium LT2 ilvYC genes were studied by fusion of each gene to the Escherichia coli K-12 galK gene. The expression of ilvY and ilvC could then be determined by measurement of the galK-encoded galactokinase enzyme. The promoter for ilvC, pC, was located by this technique to a 0.42-kilobase BglII-EcoR...

journal_title：Journal of bacteriology

authors： Blazey DL,Burns RO

更新日期：1984-09-01 00:00:00

abstract：:Several preparations of staphylococcal alpha-toxin and delta-lysin were studied in order to compare hemolytic activity with capacity to lyse bacterial protoplasts. delta-Lysin in relatively low concentration lysed protoplasts of Sarcina lutea, protoplasts of Streptococcus faecalis, and spheroplasts of Escherichia coli...

journal_title：Journal of bacteriology

authors： Bernheimer AW,Avigad LS,Grushoff P

更新日期：1968-08-01 00:00:00

abstract：:The effect of various salts on the autolysis of cell wall of a ribitol teichoic acid-deficient mutant of Staphylococcus aureus H (strain 52A5 carrying tar-1) was compared with the parent strain. In the presence of high concentrations of certain salts such as 1.0 m NaCl, the mutant undergoes autolysis with the release ...

journal_title：Journal of bacteriology

authors： Gilpin RW,Chatterjee AN,Young FE

更新日期：1972-07-01 00:00:00