Alpha-l-arabinofuranosidase from Rhodotorula flava.

Abstract:

:An alpha-L-arabinofuranosidase (EC 3.2.1.55) from the culture fluid of Rhodotorula flava IFO 0407 grown on beet arabinan as a carbon source has been highly purified. The purified enzyme has a pH optimum of 2.0. The enzyme is unusually acid stable, retaining 82% of its activity after being maintained for 24 h at pH 1.5 and at 30 degrees C. The apparent Km and Vmax values of the enzyme for phenyl alpha-L-arabinofuranoside were determined to be 9.1 mM and 72.5 mumol per min per mg of protein, respectively.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Uesaka E,Sato M,Raiju M,Kaji A

doi

10.1128/JB.133.3.1073-1077.1978

subject

Has Abstract

pub_date

1978-03-01 00:00:00

pages

1073-7

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

133

pub_type

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