Abstract:
:The ftsH gene encodes an ATP- and Zn(2+)-dependent metalloprotease which is anchored to the cytoplasmic membrane via two transmembrane segments in such a way that the very short amino- and the long carboxy termini are exposed to the cytoplasm. Deletion of the ftsH gene in Bacillus subtilis results in a pleiotropic phenotype such as filamentous growth. This observation prompted us to ask whether ftsH is involved in cell division. A translational fusion was constructed between the complete coding region of ftsH and gfp(+) the latter carrying five point mutations to obtain enhanced fluorescence. We detected that the FtsH protein accumulates in the midcell septum of dividing cells, and during sporulation first in the asymmetrically located septa of sporulating cells and later in the membrane which engulfs the forespore. These observations revealed a new function of FtsH.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Wehrl W,Niederweis M,Schumann Wdoi
10.1128/jb.182.13.3870-3873.2000keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
3870-3issue
13eissn
0021-9193issn
1098-5530journal_volume
182pub_type
杂志文章abstract::Paenibacillus mucilaginosus is a ubiquitous functional bacterium in microbial fertilizer. Here we report the complete sequence of P. mucilaginosus 3016. Multiple sets of functional genes have been found in the genome. To the best of our knowledge, this is the first announcement about the complete genome sequence of a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00323-12
更新日期:2012-05-01 00:00:00
abstract::Succinyl-coenzyme A synthetase of Escherichia coli has an alpha 2 beta 2 subunit structure. By measuring reconstituted enzyme activity present after addition of purified alpha or beta subunits to cell extracts followed by refolding, we have shown that extracts contain no significant excess of either subunit species. T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.231-237.1980
更新日期:1980-07-01 00:00:00
abstract::The two-component system SaeRS of Staphylococcus aureus is closely involved in the regulation of major virulence factors. However, little is known about the signals leading to saeRS activation. A total of four overlapping transcripts (T1 to T4) from three different transcription starting points are expressed in the sa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01927-07
更新日期:2008-05-01 00:00:00
abstract::Ikeda, Y. (University of Tokyo, Tokyo, Japan) and T. Iijima. Heterocaryosis-like phenomenon in Bacillus subtilis. J. Bacteriol. 83:1025-1028. 1962.-When a mixture of methionine-dependent, histidine-dependent, streptomycin-resistant cells and tryptophan-dependent, streptomycin-sensitive cells of Bacillus subtilis K is ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.5.1025-1028.1962
更新日期:1962-05-01 00:00:00
abstract::The nitrogenase proteins from eight organisms have been highly purified, and a survey of their cross-reactions shows that the nitrogenase proteins from a wide variety of organisms can interact with one another. An active cross-reaction is the complementary functioning of the MoFe protein and the Fe protein from differ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.3.936-943.1978
更新日期:1978-06-01 00:00:00
abstract::We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.14.4643-4645.1997
更新日期:1997-07-01 00:00:00
abstract::Burkholderia cepacia AC1100 metabolizes 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) via formation of 5-chlorohydroxyquinol (5-CHQ), hydroxyquinol (HQ), maleylacetate, and beta-oxoadipate. The step(s) leading to the dechlorination of 5-CHQ to HQ has remained unidentified. We demonstrate that a dechlorinating enzyme, Tf...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.17.4667-4675.1998
更新日期:1998-09-01 00:00:00
abstract::Protoanemonin is a toxic metabolite which may be formed during the degradation of some chloroaromatic compounds, such as polychlorinated biphenyls, by natural microbial consortia. We show here that protoanemonin can be transformed by dienelactone hydrolase of Pseudomonas sp. strain B13 to cis-acetylacrylate. Although ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.2.400-402.1998
更新日期:1998-01-01 00:00:00
abstract::Two cell shape-determining genes of Escherichia coli K-12, pbpA, the structural gene for penicillin-binding protein 2, and rodA, whose protein is unknown, were subcloned into plasmid vectors from the transducing phage lambda MAd lip24, which carries the lip-leuS region of the E. coli chromosome. Plasmids with restrict...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.10-16.1983
更新日期:1983-04-01 00:00:00
abstract::Elongation factor P (EF-P) facilitates the translation of certain peptide motifs, including those with multiple proline residues. EF-P must be posttranslationally modified for full functionality; in enterobacteria, this is accomplished by two enzymes, namely, EpmA and EpmB, which catalyze the β-lysylation of EF-P at a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00722-18
更新日期:2019-05-08 00:00:00
abstract::Two plasmids containing the penicillinase gene of alkalophilic Bacillus sp. strain 170, pEAP1 and pEAP2, were constructed. Most of the penicillinase produced by Escherichia coli, which carried these plasmids, was found in the culture medium. This excretion is caused by the cloned DNA fragment which contains some compo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.2.949-951.1983
更新日期:1983-11-01 00:00:00
abstract::Injection of mice with a sublethal dose of endotoxin 2 hr after administration of 0.1 ml of carbon tetrachloride (CCl(4)) killed 75% of the animals. CCl(4) alone killed no controls. Significant protection against this effect was afforded by 5 mg of cortisone and by 5 mg of nicotinamide adenine dinucleotide (NAD). With...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.6.1817-1823.1967
更新日期:1967-12-01 00:00:00
abstract::The ability of Rickettsia prowazekii to transport potential sources of the glucose moiety of bacterial polysaccharides was determined. Transport was determined both by filtration assays and by centrifugation through nonaqueous layers. Uridine 5'-diphosphoglucose (UDPG) was transported, whereas glucose was not transpor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.805-808.1986
更新日期:1986-09-01 00:00:00
abstract::To relate the roles of Escherichia coli SSB in recombination in vivo and in vitro, we have studied the mutant proteins SSB-1 and SSB-113, the variant SSBc produced by chymotryptic cleavage, the partially homologous variant F SSB (encoded by the E. coli sex factor), and the protein encoded by gene 32 of bacteriophage T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3422-3428.1987
更新日期:1987-08-01 00:00:00
abstract::The Bacillus subtilis enzymes ExoA and Nfo (originally termed YqfS) are endonucleases that can repair apurinic/apyrimidinic (AP) sites and strand breaks in DNA. We have analyzed how the lack of ExoA and Nfo affects the resistance of growing cells and dormant spores of B. subtilis to a variety of treatments, some of wh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.21.7374-7381.2005
更新日期:2005-11-01 00:00:00
abstract::A leucine-requiring, rifampin-sensitive strain of Neisseria gonorrhoeae was transformed to a leucine-nonrequiring, rifampin-resistant phenotype with deoxyribonucleic acid (DNA) obtained from both N. meningitidis and N. gonorrhoeae. The transforming efficiency of the meningococcal DNA was about 10- to 100-fold less tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.471-474.1975
更新日期:1975-02-01 00:00:00
abstract::Lipid composition was studied in two strains each of mid-log phase cells of Bacillus thuringiensis, B. larvae, B. popilliae, B. alvei, and B. lentimorbus. Total lipids varied from 2.5 to 3.5% of the cell dry weight of B. thuringiensis to 4.3 to 5.0% of B. popilliae. Phospholipids in the organisms examined ranged from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.3.1246-1253.1970
更新日期:1970-12-01 00:00:00
abstract::Deletions of the Escherichia coli K-12 chromosome between trpR and thr render the bacterium sensitive to the dye toluidine blue (Dye-), and if male (Hfr or F'), the strain is sterile (Fex-), failing to donate F' or chromosomal markers and resistant to male-specific phages as a consequence of its inability to elaborate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1309-1314.1983
更新日期:1983-06-01 00:00:00
abstract::Stevens, Jack G. (University of Washington, Seattle), and Neal B. Groman. Infectious bovine rhinotracheitis virus replication, cytopathology, and plaque formation in the presence and absence of nucleic acid analogues. J. Bacteriol. 87:446-453. 1964.-Cytopathology induced by infectious bovine rhinotracheitis (IBR) viru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.2.446-453.1964
更新日期:1964-02-01 00:00:00
abstract::Grunberg, E. (Hoffman-La Roche, Inc., Nutley, N.J.), and R. Cleeland. Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate. J. Bacteriol. 92:23-27. 1966.-Washed cell suspensions of Proteus mirabilis, under the proper conditions, stained well with fluorescein isothiocyanate w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.1.23-27.1966
更新日期:1966-07-01 00:00:00
abstract::The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope perme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1393-1401.1969
更新日期:1969-12-01 00:00:00
abstract::Synchrony in phototrophic cultures of Rhodospirillum rubrum was induced by stationary-phase cycling or by alterations in light intensity. Intracytoplasmic chromatophore membranes were prepared by differential centrifugation. Analysis of the composition of chromatophores obtained from cells at different times indicated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.818-823.1986
更新日期:1986-06-01 00:00:00
abstract::Antiserum was made against a single 30S protein, 30S-7. The amount of complement fixed by total 30S protein in the presence of this antiserum indicated that protein 30S-7 was the major antigen in the mixture of proteins. Each of the 30S ribosomal proteins was tested for cross-reactivity with anti-30S-7. This was done ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.2.910-915.1971
更新日期:1971-11-01 00:00:00
abstract::A procedure was developed for purification of ornithine transcarbamylase (OTCase) to near homogeneity from Bacillus subtilis 168. The purified native enzyme existed as a mixture of dimeric, tetrameric, and hexameric forms, but was converted to the dimer in the presence of 2-mercaptoethanol. The molecular weight of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.512-521.1983
更新日期:1983-08-01 00:00:00
abstract::The Tm of Bacillus stearothermophilus 5S ribonucleic acid (RNA) is 1.5 +/- 0.5 C higher than that of 5S RNAs from B. subtilis and Escherichia coli. Melting in 50% methanol and in formaldehyde indicate that both base stacking and helical regions are involved in the slightly increased thermal stability of B. stearotherm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.850-854.1976
更新日期:1976-03-01 00:00:00
abstract::Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3448-3452.1998
更新日期:1998-07-01 00:00:00
abstract::Antibiotic resistance in Neisseria gonorrhoeae has been associated with the acquisition of R plasmids from heterologous organisms. The broad-host-range plasmids of incompatibility groups P (IncP) and Q (IncQ) have played a role in this genetic exchange in nature. We have utilized derivatives of RSF1010 (IncQ) and RP1 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.18.5793-5799.1991
更新日期:1991-09-01 00:00:00
abstract::The potent tumor-promoting agent croton oil, which has been shown previously to be strongly mitogenic in mammalian cells, stimulates cell division in snake mutants of the blue-green bacterium Agmenellum quadruplicatum. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.874-875.1973
更新日期:1973-05-01 00:00:00
abstract::Henle, Gertrude (Children's Hospital of Philadelphia, Philadelphia, Pa.), and Werner Henle. Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bacteriol. 91:1248-1256. 1966.-Indirect immunofluorescence tests led to the brilliant staining of a small proportion of the cells in five different cultures derive...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1248-1256.1966
更新日期:1966-03-01 00:00:00
abstract::MntH, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (Nramp1), is a primary transporter for Mn(2+) influx in Salmonella enterica serovar Typhimurium and Escherichia coli. S. enterica serovar Typhimurium MntH contributes to H(2)O(2) resistance and is important for full virulence. Co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.12.3151-3158.2002
更新日期:2002-06-01 00:00:00