The FtsH protein accumulates at the septum of Bacillus subtilis during cell division and sporulation.

Abstract:

:The ftsH gene encodes an ATP- and Zn(2+)-dependent metalloprotease which is anchored to the cytoplasmic membrane via two transmembrane segments in such a way that the very short amino- and the long carboxy termini are exposed to the cytoplasm. Deletion of the ftsH gene in Bacillus subtilis results in a pleiotropic phenotype such as filamentous growth. This observation prompted us to ask whether ftsH is involved in cell division. A translational fusion was constructed between the complete coding region of ftsH and gfp(+) the latter carrying five point mutations to obtain enhanced fluorescence. We detected that the FtsH protein accumulates in the midcell septum of dividing cells, and during sporulation first in the asymmetrically located septa of sporulating cells and later in the membrane which engulfs the forespore. These observations revealed a new function of FtsH.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wehrl W,Niederweis M,Schumann W

doi

10.1128/jb.182.13.3870-3873.2000

keywords:

subject

Has Abstract

pub_date

2000-07-01 00:00:00

pages

3870-3

issue

13

eissn

0021-9193

issn

1098-5530

journal_volume

182

pub_type

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