Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate.

Abstract:

:Grunberg, E. (Hoffman-La Roche, Inc., Nutley, N.J.), and R. Cleeland. Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate. J. Bacteriol. 92:23-27. 1966.-Washed cell suspensions of Proteus mirabilis, under the proper conditions, stained well with fluorescein isothiocyanate with little or no loss of cell viability. The speed and intensity of the reaction was dependent on both the concentration of dye and pH. Within a range of pH 3.0 to 10.0, staining was most rapid at pH 5.0 to 6.0, with a slower and less intense reaction occurring at the other pH values. As the concentration of dye at either pH 5.0 or 9.0 was increased from 10 to 1,000 mug/ml, there was an increase in the rate of staining but a decrease in cell viability. After 24 hr of incubation at 4 C, pH 5.0, and a dye concentration of 10 mug/ml, all cells were stained, the majority exhibiting intense fluorescence with little or no loss of viability noted. In preliminary experiments with Staphylococcus aureus, similar results were obtained. Of various other fluorescent dyes tested, only rhodamine isothiocyanate was found to give satisfactory staining.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Grunberg E,Cleeland R

doi

10.1128/JB.92.1.23-27.1966

subject

Has Abstract

pub_date

1966-07-01 00:00:00

pages

23-7

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

92

pub_type

杂志文章
  • Protein and fatty acid composition of mesosomal vesicles and plasma membranes of Staphylococcus aureus.

    abstract::Qualitatively, the protein and fatty acid composition of purified mesosomal vesicles and the plasma membrane isolated from Staphylococcus aureus ATCC 6538P are identical, the major difference between these two cellular components being only quantitative in nature. Mesosomal vesicles and plasma membranes, when subjecte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.571-576.1973

    authors: Theodore TS,Panos C

    更新日期:1973-11-01 00:00:00

  • Regulation of a common amidotransferase subunit.

    abstract::In Bacillus subtilis the trpX locus specifies a glutamine-binding protein designated subunit X, which forms a complex with subunit E to constitute the anthranilate synthase enzyme aggregate (EX) and subunit A to constitute the p-aminobenzoate synthase enzyme aggregate (AX). Subunit X confers upon these enzyme complexe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.419-425.1977

    authors: Kane JF

    更新日期:1977-11-01 00:00:00

  • Regulation of ribonucleoside diphosphate reductase synthesis in Escherichia coli: increased enzyme synthesis as a result of inhibition of deoxyribonucleic acid synthesis.

    abstract::Inhibition of deoxyribonucleic acid (DNA) synthesis in Escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnaE and dnaB) or initiation (dnaA) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.107-113.1977

    authors: Filpula D,Fuchs JA

    更新日期:1977-04-01 00:00:00

  • Genetic and Proteomic Analyses of Pupylation in Streptomyces coelicolor.

    abstract:UNLABELLED:Pupylation is a posttranslational modification peculiar to actinobacteria wherein proteins are covalently modified with a small protein called the prokaryotic ubiquitin-like protein (Pup). Like ubiquitination in eukaryotes, this phenomenon has been associated with proteasome-mediated protein degradation in m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00302-15

    authors: Compton CL,Fernandopulle MS,Nagari RT,Sello JK

    更新日期:2015-09-01 00:00:00

  • Factors affecting cellulolysis by Ruminococcus albus.

    abstract::The factors influencing the digestion of pebble-milled cellulose by enzymes were studied by using several strains of Ruminococcus albus including a mutant characterized by a more eccentric location of its colony in the clearing produced by digestion of the cellulose in the thin layer lining the wall of a culture tube....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.729-737.1973

    authors: Smith WR,Yu I,Hungate RE

    更新日期:1973-05-01 00:00:00

  • Characterization of pyridoxine auxotrophs of Escherichia coli: serine and pdxF mutants.

    abstract::At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.2.658-667.1970

    authors: Dempsey WB,Ito H

    更新日期:1970-11-01 00:00:00

  • Correlation between polyploidy and auxotrophic segregation in the imperfect yeast Candida albicans.

    abstract::In order to clarify the relationship between polyploidization and the capability of phenotypic switching in the imperfect yeast Candida albicans, two types of variants were isolated as segregants from a fusant, which produced a proportion of the cell population with a higher ploidy than the rest, either in a temperatu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.11.3345-3353.1994

    authors: Suzuki T,Hitomi A,Magee PT,Sakaguchi S

    更新日期:1994-06-01 00:00:00

  • Primary structure of cyanelle peptidoglycan of Cyanophora paradoxa: a prokaryotic cell wall as part of an organelle envelope.

    abstract::The peptidoglycan layer surrounding the photosynthetic organelles (cyanelles) of the protist Cyanophora paradoxa is thought to be a relic of their cyanobacterial ancestors. The separation of muropeptides by gel filtration and reverse-phase high-performance liquid chromatography revealed four different muropeptide mono...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.2.332-339.1996

    authors: Pfanzagl B,Zenker A,Pittenauer E,Allmaier G,Martinez-Torrecuadrada J,Schmid ER,De Pedro MA,Löffelhardt W

    更新日期:1996-01-01 00:00:00

  • Methionine sulfoxide reductase from the hyperthermophilic archaeon Thermococcus kodakaraensis, an enzyme designed to function at suboptimal growth temperatures.

    abstract::Methionine sulfoxide reductase (Msr) catalyzes the thioredoxin-dependent reduction and repair of methionine sulfoxide (MetO). Although Msr genes are not present in most hyperthermophile genomes, an Msr homolog encoding an MsrA-MsrB fusion protein (MsrAB(Tk)) was present on the genome of the hyperthermophilic archaeon ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00751-06

    authors: Fukushima E,Shinka Y,Fukui T,Atomi H,Imanaka T

    更新日期:2007-10-01 00:00:00

  • Mechanism of tuberculostasis in mammalian serum. II. Induction of serum tuberculostasis in guinea pigs.

    abstract::The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.64-70.1969

    authors: Kochan I,Golden CA,Bukovic JA

    更新日期:1969-10-01 00:00:00

  • Induction of experimental chronic arthritis in rabbits by cell-free fragments of Erysipelothrix.

    abstract::A cell-free crude extract of Erysipelothrix rhusiopathiae injected by high pressure jet into the knee-joint of rabbits stimulated an acute, mild inflammatory reaction. Additional injections at 3-day intervals induced a chronic condition characterized by hyperplasia of the synovial cells and hypertrophy of the villi, d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.403-406.1969

    authors: White TG,Puls JL

    更新日期:1969-05-01 00:00:00

  • Heat reactivation of the alpha-hemolytic, dermonecrotic, lethal activities of crude and purified staphylococcal alpha-toxin.

    abstract::Manohar, M. (University of Minnesota, St. Paul), S. Kumar, and R. K. Lindorfer. Heat reactivation of the alpha-hemolytic, dermonecrotic, and lethal activities of crude and purified staphylococcal alpha-toxin. J. Bacteriol. 91:1681-1685. 1966.-Crude staphylococcal toxin loses its alpha-hemolytic activity more rapidly a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.5.1681-1685.1966

    authors: Manohar M,Kumar S,Lindorfer RK

    更新日期:1966-05-01 00:00:00

  • Characterization of Tn5 mutants deficient in dissimilatory nitrite reduction in Pseudomonas sp. strain G-179, which contains a copper nitrite reductase.

    abstract::Tn5 was used to generate mutants that were deficient in the dissimilatory reduction of nitrite for Pseudomonas sp. strain G-179, which contains a copper nitrite reductase. Three types of mutants were isolated. The first type showed a lack of growth on nitrate, nitrite, and nitrous oxide. The second type grew on nitrat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.20.6653-6658.1992

    authors: Ye RW,Averill BA,Tiedje JM

    更新日期:1992-10-01 00:00:00

  • Transcriptional regulation of delta-aminolevulinic acid dehydratase synthesis by oxygen in Bradyrhizobium japonicum and evidence for developmental control of the hemB gene.

    abstract::An increased demand for cytochromes is associated with symbiotic development and microaerobic metabolism in the bacterium Bradyrhizobium japonicum, and evidence suggests that hemB, rather than hemA, is the first essential bacterial heme synthesis gene in symbiosis with soybean. Steady-state levels of mRNA and protein ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.11.3706-3710.1997

    authors: Chauhan S,O'Brian MR

    更新日期:1997-06-01 00:00:00

  • Denitrification by Alcaligenes eutrophus is plasmid dependent.

    abstract::Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.852-854.1985

    authors: Römermann D,Friedrich B

    更新日期:1985-05-01 00:00:00

  • The cytochrome bc1 complex of Rhodobacter sphaeroides can restore cytochrome c2-independent photosynthetic growth to a Rhodobacter capsulatus mutant lacking cytochrome bc1.

    abstract::Plasmids encoding the structural genes for the Rhodobacter capsulatus and Rhodobacter sphaeroides cytochrome (cyt) bc1 complexes were introduced into strains of R. capsulatus lacking the cyt bc1 complex, with and without cyt c2. The R. capsulatus merodiploids contained higher than wild-type levels of cyt bc1 complex, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.11.6059-6068.1989

    authors: Davidson E,Prince RC,Haith CE,Daldal F

    更新日期:1989-11-01 00:00:00

  • Genetics of methyl-accepting chemotaxis proteins in Escherichia coli: cheD mutations affect the structure and function of the Tsr transducer.

    abstract::The tsr gene specifies a methyl-accepting membrane protein involved in chemotaxis to serine and several repellent compounds. We have characterized a special class of tsr mutations designated cheD which alter the signaling properties of the Tsr transducer. Unlike tsr null mutants, cheD strains are generally nonchemotac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.96-104.1985

    authors: Callahan AM,Parkinson JS

    更新日期:1985-01-01 00:00:00

  • Effect of carbon source and the role of cyclic adenosine 3',5'-monophosphate on the Caulobacter cell cycle.

    abstract::The expression of cell cycle events in Caulobacter crescentus CB13 has been shown to be associated with regulation of carbohydrate utilization. Growth on lactose and galactose depends on induction of specific enzymes. Prior growth on glucose results in a delay in enzyme expression and cell cycle arrest at the nonmotil...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.3.951-959.1977

    authors: Kurn N,Shapiro L,Agabian N

    更新日期:1977-09-01 00:00:00

  • Bacteriophage clearance in the oyster (Crassotrea virginica).

    abstract::Since relatively few quantitative immunological studies had been conducted with invertebrates, this study was designed to learn more about the immune potential of the oyster, a molluscan representative. Experiments measured the primary and secondary clearance rates of T2 coliphage in the oyster. The fate of phage inje...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.4.1260-1266.1968

    authors: Acton RT,Evans EE

    更新日期:1968-04-01 00:00:00

  • Depression of guanosine diphosphate-mannose pyrophosphorylase by mutations in two different regulator genes involved in capsular polysaccharide synthesis in Escherichia coli K-12.

    abstract::Mutations in a regulator gene (capR) that causes increased synthesis of capsular polysaccharide and derepressed synthesis of several enzymes involved in polysaccharide synthesis also derepress synthesis of guanosine diphosphate (GDP)-mannose pyrophosphorylase. In addition, a second mucoid mutation (capS, which maps se...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.3.965-972.1970

    authors: Lieberman MM,Markovitz A

    更新日期:1970-03-01 00:00:00

  • Genetic transformation of Streptococcus pneumoniae by heterologous plasmid deoxyribonucleic acid.

    abstract::A number of heterologous plasmid deoxyribonucleic acids (DNAs) coding for erythromycin, tylosin, lincomycin, tetracycline, or chloramphenicol resistance have been introduced into Streptococcus pneumoniae via genetic transformation with frequencies that varied between 10(-5) to as high as 5 x 10(-1) per colony-forming ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.2.698-709.1980

    authors: Barany F,Tomasz A

    更新日期:1980-11-01 00:00:00

  • Identification of a complex operator for galP1, the glucose-sensitive, galactose-dependent promoter of the Streptomyces galactose operon.

    abstract::The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.5.1213-1220.1993

    authors: Mattern SG,Brawner ME,Westpheling J

    更新日期:1993-03-01 00:00:00

  • Effect of environmental pH on morphological development of Candida albicans is mediated via the PacC-related transcription factor encoded by PRR2.

    abstract::The ability to respond to ambient pH is critical to the growth and virulence of the fungal pathogen Candida albicans. This response entails the differential expression of several genes affecting morphogenesis. To investigate the mechanism of pH-dependent gene expression, the C. albicans homolog of pacC, designated PRR...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.24.7524-7530.1999

    authors: Ramon AM,Porta A,Fonzi WA

    更新日期:1999-12-01 00:00:00

  • Promoters recognized by Escherichia coli RNA polymerase selected by function: highly efficient promoters from bacteriophage T5.

    abstract::Highly efficient promoters of coliphage T5 were identified by selecting for functional properties. Eleven such promoters belonging to all three expression classes of the phage were analyzed. Their average AT content was 75% and reached 83% in subregions of the sequences. Besides the well-known conserved sequences arou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.70-77.1985

    authors: Gentz R,Bujard H

    更新日期:1985-10-01 00:00:00

  • Two-component sensor required for normal symbiotic colonization of euprymna scolopes by Vibrio fischeri.

    abstract::The light organ of the squid Euprymna scolopes is specifically colonized to a high density by the marine bacterium Vibrio fischeri. To date, only a few factors contributing to the specificity of this symbiosis have been identified. Using a genetic screen for random transposon mutants defective in initiating the symbio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.3.835-842.2001

    authors: Visick KL,Skoufos LM

    更新日期:2001-02-01 00:00:00

  • Growth phase and pH influence peptide signaling for competence development in Streptococcus mutans.

    abstract::The development of competence by the dental caries pathogen Streptococcus mutans is mediated primarily through the alternative sigma factor ComX (SigX), which is under the control of multiple regulatory systems and activates the expression of genes involved in DNA uptake and recombination. Here we report that the indu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00995-13

    authors: Guo Q,Ahn SJ,Kaspar J,Zhou X,Burne RA

    更新日期:2014-01-01 00:00:00

  • Expression of the Bacillus subtilis acsA gene: position and sequence context affect cre-mediated carbon catabolite repression.

    abstract::In Bacillus subtilis, carbon catabolite repression (CCR) of many genes is mediated at cis-acting carbon repression elements (cre) by the catabolite repressor protein CcpA. Mutations in transcription-repair coupling factor (mfd) partially relieve CCR at cre sites located downstream of transcriptional start sites by abo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.24.6649-6654.1998

    authors: Zalieckas JM,Wray LV Jr,Fisher SH

    更新日期:1998-12-01 00:00:00

  • A pH 7 buffer devoid of nitrogen, sulfur, and phosphorus for use in bacteriological systems.

    abstract::Apparent pK values were determined for a series of commercially available carboxylic acids in a search for a buffer suitable for bacterial studies at pH 7 in media free from nitrogen, sulfur, and phosphorus. Of the compounds possessing pK' values near pH 7,3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid (EMTA) was c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.2.283-290.1967

    authors: Mallette MF

    更新日期:1967-08-01 00:00:00

  • Detection and quantification of superoxide formed within the periplasm of Escherichia coli.

    abstract::Many gram-negative bacteria harbor a copper/zinc-containing superoxide dismutase (CuZnSOD) in their periplasms. In pathogenic bacteria, one role of this enzyme may be to protect periplasmic biomolecules from superoxide that is released by host phagocytic cells. However, the enzyme is also present in many nonpathogens ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00554-06

    authors: Korshunov S,Imlay JA

    更新日期:2006-09-01 00:00:00

  • Medium-dependent alteration of lysine transfer ribonucleic acid in sporulating Bacillus subtilis cells.

    abstract::The presence of the altered lysine transfer ribonucleic acid (tRNA) in Bacillus subtilis spores is strongly dependent on the medium on which the cells were sporulated. Cells sporulated on synthetic media or dilute complex media contain little or none of the new component, whereas those sporulated on concentrated compl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.125-130.1967

    authors: Lazzarini RA,Santangelo E

    更新日期:1967-07-01 00:00:00