Characterization of pyridoxine auxotrophs of Escherichia coli: serine and pdxF mutants.

Abstract:

:At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The aminotransferase isolated from this mutant has been compared to that isolated from wild-type E. coli and found to have apparently normal affinity for pyridoxal 5'-phosphate, but reduced affinity for pyridoxamine 5'-phosphate.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Dempsey WB,Ito H

doi

10.1128/JB.104.2.658-667.1970

subject

Has Abstract

pub_date

1970-11-01 00:00:00

pages

658-67

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

104

pub_type

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