Abstract:
:The minimal origin of replication of the broad-host-range plasmid RK2 has two potential recognition sequences for the DnaA protein of Escherichia coli. DNA transfer by transformation into a dnaA-null mutant of E. coli showed that DnaA protein is needed for replication or maintenance of mini-RK2. We isolated and purified DnaA protein as a chimeric protein, covalently attached to a piece of collagen and beta-galactosidase. The hybrid protein specifically bound to restriction fragments from the oriV region of RK2, which contained the two dnaA boxes. Deletion of the second dnaA box inactivated the origin and abolished the binding of the hybrid protein to the DNA fragment that had suffered the deletion. When the second dnaA box was replaced with an EcoRI linker of identical length, origin activity was restored. Binding experiments showed that the linker provided a weak dnaA box. An alternative explanation was that the linker restored proper spacing between sequences on either side of the deleted box, thus restoring origin activity.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gaylo PJ,Turjman N,Bastia Ddoi
10.1128/jb.169.10.4703-4709.1987subject
Has Abstractpub_date
1987-10-01 00:00:00pages
4703-9issue
10eissn
0021-9193issn
1098-5530journal_volume
169pub_type
杂志文章abstract::Strain 768-28 was isolated from a hot spring in Kamchatka, Russia, and represents a novel member of the Vulcanisaeta genus. The complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the Embden-Meyerhof and Entner-Doudoroff pathways for gluc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00237-11
更新日期:2011-05-01 00:00:00
abstract::The catalase gene, katA, of the sepiolid squid symbiont Vibrio fischeri has been cloned and sequenced. The predicted amino acid sequence of KatA has a high degree of similarity to the recently defined group III catalases, including those found in Haemophilus influenzae, Bacteroides fragilis, and Proteus mirabilis. Ups...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.8.2087-2092.1998
更新日期:1998-04-01 00:00:00
abstract::We have extended our previous studies of the DNA sequences required for growth rate-dependent control of rRNA transcription in Escherichia coli. Utilizing a reporter system suitable for evaluation of promoters with low activities, we have found that the core promoter region of rrnB P1 (-41 to +1 with respect to the tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.17.5560-5564.1994
更新日期:1994-09-01 00:00:00
abstract::Accumulation of abnormal proteins in cells of bacteria or eukaryotes can induce synthesis of a set of heat shock proteins. We examined such induction following addition of azetidine (a proline analog) or synthesis of a heterologous protein (human prourokinase) in Escherichia coli. Synthesis of heat shock proteins unde...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5648-5653.1994
更新日期:1994-09-01 00:00:00
abstract::Generally nonchemotactic mutants of Escherichia coli and Salmonella typhimurium were analyzed by interspecies complementation tests to determine the functional correspondence between the che genes of these two organisms. The E. coli che region was introduced into Salmonella recipients by means of a series of F-prime e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.1.107-114.1979
更新日期:1979-07-01 00:00:00
abstract::The cell walls isolated from axenically grown leprosy-derived corynebacteria were submitted to various chemical and enzymatic degradations. The glycan strands of the wall peptidoglycan are essentially composed of N-acetylglycosaminyl-N-acetylmuramic acid disaccharide units. Small amounts of N-acetylglycosaminyl-N-glyc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.775-779.1981
更新日期:1981-02-01 00:00:00
abstract::Constitutive beta-glucosidases from Saccharomyces fragilis (Y-18) and S. dobzhanskii (Y-19) precipitated at the same concentration of ammonium sulfate. The partially purified enzymes had similar activation energies, molecular weights, affinities for certain natural and synthetic beta-glucosides, and optimal pH values ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.135-141.1967
更新日期:1967-01-01 00:00:00
abstract::Signal transduction protein P(II) is dephosphorylated in Synechocystis sp. strain PCC 6803 by protein phosphatase PphA. To determine the impact of PphA-mediated P(II) dephosphorylation on physiology, the phenotype of a PphA-deficient mutant was analyzed. Mutants lacking either PphA or P(II) were impaired in efficient ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.19.6683-6690.2005
更新日期:2005-10-01 00:00:00
abstract::This paper describes the major transmutations of donor deoxyribonucleic acid (DNA) after uptake by competent Bacillus subtilis cells. Kinetic experiments confirm that after exposure to competent cells, donor DNA is converted to double-stranded fragments (DSF) which can be isolated as early as 30 s from the beginning o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.1.154-162.1973
更新日期:1973-10-01 00:00:00
abstract::The chpB locus is a chromosomal homolog of the pem locus, which is responsible for stable maintenance of plasmid R100 within the host cells. Like pem, chpB codes for two genes, chpBK and chpBI, encoding a growth inhibitor and a suppressor for the killing action of the ChpBK protein, respectively. Here, we determined t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5861-5863.1994
更新日期:1994-09-01 00:00:00
abstract::Escherichia coli acetyl coenzyme A carboxylase (ACC), the first enzyme of the fatty acid biosynthetic pathway, is inhibited by acylated derivatives of acyl carrier protein (ACP). ACP lacking an acyl moiety does not inhibit ACC. Acylated derivatives of ACP having chain lengths of 6 to 20 carbon atoms were similarly inh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.4.1499-1503.2001
更新日期:2001-02-01 00:00:00
abstract::Coscarelli, Waldimero (Rutgers, The State University, New Brunswick, N.J.) and David Pramer. Nutrition and growth of Arthrobotrys conoides. J. Bacteriol. 84:60-64. 1962-Despite its ability to capture, kill, and subsist on nematodes, the predacious fungus Arthrobotrys conoides does not demonstrate a unique nutritional ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.60-64.1962
更新日期:1962-07-01 00:00:00
abstract::The antibiotic d-cycloserine is an effective inhibitor of alanine racemase. The lack of inhibition by l-cycloserine of alanine racemase from Staphylococcus aureus led Roze and Strominger to formulate the cycloserine hypothesis. This hypothesis states that d-cycloserine has the conformation required of the substrates o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.978-987.1972
更新日期:1972-06-01 00:00:00
abstract::The uropathogenic Escherichia coli strain 536 carries at least five genetic elements on its chromosome that meet all criteria characteristic of pathogenicity islands (PAIs). One main feature of these distinct DNA regions is their instability. We applied the so-called island-probing approach and individually labeled al...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.10.3086-3096.2004
更新日期:2004-05-01 00:00:00
abstract::The mannans of Saccharomyces chevalieri, S. italicus, S. diastaticus, and S. carlsbergensis, were acetolyzed, and the fragments were separated by gel filtration. All gave similar acetolysis fingerprints, which were distinguished from S. cerevisiae by the presence of a pentasaccharide component in addition to the mono-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.2.461-467.1974
更新日期:1974-02-01 00:00:00
abstract::Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus. While recA mutants in most bacterial species are hypersensitive to UV damage, surprisingly a B. abortus recA null mutant ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01994-05
更新日期:2006-07-01 00:00:00
abstract::Sguros, Peter L. (University of Miami, Miami, Fla.), and Jacqueline Simms. Role of marine fungi in the biochemistry of the oceans. IV. Growth responses to seawater inorganic macroconstituents. J. Bacteriol. 88:346-355. 1964.-The filamentous ascomycete Halosphaeria mediosetigera and the deuteromycete Culcitalna achrasp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.2.346-355.1964
更新日期:1964-08-01 00:00:00
abstract::Englesberg, E. (University of Pittsburgh, Pittsburgh, Pa.), R L. Anderson, R. Weinberg, N. Lee, P. Hoffee, G. Huttenhauer, and H. Boyer. l-Arabinose-sensitive, l-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J. Bacteriol. 84:137-146. 1962-l-Arabinose-negative mutants of Escherichia coli B/r, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.137-146.1962
更新日期:1962-07-01 00:00:00
abstract::Growth of Escherichia coli B in simple media enriched with Mn(II) resulted in the elevation of the manganese-containing superoxide dismutase, whereas growth in such medium enriched with iron caused increased content of the iron-containing superoxide dismutase. Enrichment of the medium with Co(II), Cu(II), Mo(VI), Zn(I...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.137-142.1984
更新日期:1984-10-01 00:00:00
abstract::This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.3.808-820.1978
更新日期:1978-06-01 00:00:00
abstract::A mutant (designated mec(-)) of Escherichia coli F(+) 100 endo I(-)su(+) r(K) (-)m(K) (+) has been isolated which is defective in cytosine-specific deoxyribonucleic acid (DNA) methylase activity. The DNA of this mutant, as well as the DNA of phages lambda and fd propagated in it, is virtually devoid of 5-methyl-cytosi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.1103-1107.1973
更新日期:1973-09-01 00:00:00
abstract::Hogg, Robert W. (University of Illinois, Urbana), Chitra S. Biswas, and Harry P. Broquist. Interference with valine and isoleucine biosynthesis by cyclic hydroxamic acids. J. Bacteriol. 90:1265-1270. 1965.-The introduction of a hydroxyl group in a number of common barbiturates, a substitution which converts these comp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.5.1265-1270.1965
更新日期:1965-11-01 00:00:00
abstract::The pH dependence of transport of the neutral amino acids L-serine and L-alanine by membrane vesicles of Streptococcus cremoris have been studied in detail. The rates of four modes of facilitated diffusion (e.g., influx, efflux, exchange, and counterflow) of L-serine and L-alanine increase with increasing H+ concentra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2748-2754.1987
更新日期:1987-06-01 00:00:00
abstract::The Escherichia coli secA gene, whose expression is responsive to the protein secretion status of the cell, is the second gene in an operon. We found that both the basal and induced levels of SecA biosynthesis are dependent on prior translation of the upstream gene, gene X, and identified two large gene X-secA transcr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.643-649.1989
更新日期:1989-02-01 00:00:00
abstract::A heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of Pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. The original bacterial culture had been obtained from a bison that died of hemorrhagi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.7-14.1967
更新日期:1967-01-01 00:00:00
abstract::The EcfG-type sigma factor RpoE2 is the regulator of the general stress response in Sinorhizobium meliloti. RpoE2 activity is negatively regulated by two NepR-type anti-sigma factors (RsiA1/A2), themselves under the control of two anti-anti-sigma factors (RsiB1/B2) belonging to the PhyR family of response regulators. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01623-14
更新日期:2014-07-01 00:00:00
abstract:UNLABELLED:CbrA is an atypical sensor kinase found in Pseudomonas. The autokinase domain is connected to a putative transporter of the sodium/solute symporter family (SSSF). CbrA functions together with its cognate response regulator, CbrB, and plays an important role in nutrient acquisition, including regulation of hu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00361-15
更新日期:2015-09-01 00:00:00
abstract::The gene coding for the metabolically stable 6S RNA of Escherichia coli has been cloned, sequenced, and partially characterized in expression analyses. The DNA sequence results confirm the accuracy of the previously established RNA sequence and, with genomic hybridization data, reveal that there is only one copy of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.3.1162-1170.1985
更新日期:1985-03-01 00:00:00
abstract::Plasmid pE194 has been shown to be rescued by integration after cultivation of infected Bacillus subtilis recE4 cells at a restrictive high temperature. The plasmid is also spontaneously excised from the chromosome at a low frequency by precise or imprecise excision (J. Hofemeister, M. Israeli-Reches, and D. Dubnau, M...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.21.6997-7002.1992
更新日期:1992-11-01 00:00:00
abstract::The potassium permeability of Rickettsia prowazekii was characterized by chemical measurement of the intracellular sodium and potassium pools and isotopic flux measurements with 86Rb+ as a tracer. R. prowazekii, in contrast to Escherichia coli, did not maintain a high potassium-to-sodium ratio in their cytoplasm excep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.197-201.1984
更新日期:1984-01-01 00:00:00