Abstract:
:Hogg, Robert W. (University of Illinois, Urbana), Chitra S. Biswas, and Harry P. Broquist. Interference with valine and isoleucine biosynthesis by cyclic hydroxamic acids. J. Bacteriol. 90:1265-1270. 1965.-The introduction of a hydroxyl group in a number of common barbiturates, a substitution which converts these compounds to cyclic hydroxamic acids, gives rise to compounds which inhibit growth of Escherichia coli. The toxicity of these hydroxybarbiturates appears to be associated, at least in part, with interference with valine and isoleucine biosynthesis, as a combination of these amino acids counteracts their toxicity. A subinhibitory level of 1-hydroxy-5-ethyl-5-isopropylbarbituric acid (hydroxyipral) was counteracted either by valine or by its early precursor, alpha-acetolactate, and led to a study of the effect of these cyclic hydroxamic acids on acetolactate synthesis in a cell-free enzyme system of E. coli. In this system, the parent barbiturates and their respective hydroxy derivatives were only moderately active in blocking acetolactate synthesis. Detailed kinetic studies of the most active compound, hydroxyipral showed no obvious relationship to the substrate or cofactors of the system. The inhibitory effects of hydroxyipral, either on growth of E. coli or in the acetolactate-forming system, could not be counteracted by Fe(++), but the toxic effect of aspergillic acid and o-phenanthroline in these instances was reversed by Fe(++), which implies an iron involvement in the acetolactate-forming system of E. coli.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hogg RW,Biswas CS,Broquist HPdoi
10.1128/JB.90.5.1265-1270.1965subject
Has Abstractpub_date
1965-11-01 00:00:00pages
1265-70issue
5eissn
0021-9193issn
1098-5530journal_volume
90pub_type
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