Effects of the Gram stain on microspheres from thermal polyamino acids.

abstract：:A cyclic version of the Entner-Doudoroff pathway is used by Pseudomonas aeruginosa to metabolize carbohydrates. Genes encoding the enzymes that catabolize intracellular glucose to pyruvate and glyceraldehyde 3-phosphate are coordinately regulated, clustered at 39 min on the chromosome, and collectively form the hex re...

journal_title：Journal of bacteriology

authors： Hager PW,Calfee MW,Phibbs PV

更新日期：2000-07-01 00:00:00

abstract：:The ruvB genes of the highly divergent thermophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expressed in Escherichia coli. Both thermostable RuvB proteins were purified to homogeneity. Like E. coli RuvB protein, both purified thermostable RuvB proteins showed strong double-s...

journal_title：Journal of bacteriology

authors： Tong J,Wetmur JG

更新日期：1996-05-01 00:00:00

abstract：:The tyrT (tRNA(1TYr)) locus of Escherichia coli B differs structurally from that of K-12 strains by the absence of 2 of 3.14 terminal repeat sequences. ...

journal_title：Journal of bacteriology

authors： Timms AR,Bridges BA

更新日期：1996-04-01 00:00:00

abstract：:The major coat protein of the bacteriophage f1 is synthesized during infection of Escherichia coli and becomes tightly associated with the host membrane. This synthesis was studied in conjunction with the strain BB26-36, a mutant defective in phospholipid synthesis, to investigate basic questions concerning membrane p...

journal_title：Journal of bacteriology

authors： Cashman JS,Webster RE

更新日期：1977-03-01 00:00:00

abstract：:Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO)...

journal_title：Journal of bacteriology

authors： Kuske CR,Kirkpatrick BC

更新日期：1990-03-01 00:00:00

abstract：:This study shows that sequential introduction of drug resistance mutations substantially increased enzyme production in Paenibacillus agaridevorans The triple mutant YT478 (rsmG Gln225→stop codon, rpsL K56R, and rpoB R485H), generated by screening for resistance to streptomycin and rifampin, expressed a 1,100-fold-lar...

journal_title：Journal of bacteriology

authors： Funane K,Tanaka Y,Hosaka T,Murakami K,Miyazaki T,Shiwa Y,Gibu S,Inaoka T,Kasahara K,Fujita N,Yoshikawa H,Hiraga Y,Ochi K

更新日期：2018-08-10 00:00:00

abstract：:Eight mutants of Bacillus subtilis deficient in xylan utilization were isolated and characterized genetically and biochemically. Each mutant was obtained independently after nitrosoguanidine mutagenesis. All of the analyzed mutations were shown to be linked. Reciprocal transformation crosses revealed the existence of ...

journal_title：Journal of bacteriology

authors： Roncero MI

更新日期：1983-10-01 00:00:00

abstract：:The crtEF, bchCA, and puf operons of the facultative phototrophic bacterium Rhodobacter capsulatus encode gene products that are necessary for the formation of various components of the photosynthetic apparatus. The crtEF operon encodes two enzymes involved in the biosynthesis of carotenoids, the bchCA operon codes fo...

journal_title：Journal of bacteriology

authors： Wellington CL,Beatty JT

更新日期：1991-02-01 00:00:00

abstract：:IcsA is an outer membrane protein in the autotransporter family that is required for Shigella flexneri pathogenesis. Following its secretion through the Sec translocon, IcsA is incorporated into the outer membrane in a process that depends on YaeT, a component of an outer membrane beta-barrel insertion machinery. We i...

journal_title：Journal of bacteriology

authors： Wagner JK,Heindl JE,Gray AN,Jain S,Goldberg MB

更新日期：2009-02-01 00:00:00

abstract：:We identified in Streptococcus mutans six new genes (rgpA through rgpF), whose disruption results in a loss of serotype-specific antigenicity, specified by the glucose side chains of rhamnose-glucose polysaccharide from the cell wall. Rhamnose and glucose content of the cell wall decreased drastically in all these dis...

journal_title：Journal of bacteriology

authors： Yamashita Y,Tsukioka Y,Tomihisa K,Nakano Y,Koga T

更新日期：1998-11-01 00:00:00

abstract：:Hyaluronate stimulated the formation of exolipase by Serratia marcescens. This ability was abolished when all carboxyl groups of hyaluronate were methyl esterified. Additional studies suggested that the biological inactivity of esterified hyaluronate should be ascribed to the reduced conformational order of the molecu...

journal_title：Journal of bacteriology

authors： Jäger KE,Winkler UK

更新日期：1979-09-01 00:00:00

abstract：:The two highly homologous cbb operons of Alcaligenes eutrophus H16 that are located on the chromosome and on megaplasmid pHG1 contain genes encoding several enzymes of the Calvin carbon reduction cycle. Sequence analysis of a region from the promoter-distal part revealed two open reading frames, designated cbbT and cb...

journal_title：Journal of bacteriology

authors： Schäferjohann J,Yoo JG,Kusian B,Bowien B

更新日期：1993-11-01 00:00:00

abstract：:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

journal_title：Journal of bacteriology

authors： Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

更新日期：2010-11-01 00:00:00

abstract：:Vibrio vulnificus is a causative agent of serious food-borne diseases in humans related to the consumption of raw seafood. It secretes a metalloprotease that is associated with skin lesions and serious hemorrhagic complications. In this study, we purified and characterized an extracellular metalloprotease (designated ...

journal_title：Journal of bacteriology

authors： Chang AK,Kim HY,Park JE,Acharya P,Park IS,Yoon SM,You HJ,Hahm KS,Park JK,Lee JS

更新日期：2005-10-01 00:00:00

abstract：:Alkaline phosphatase (APase) is induced as a culture enters stationary phase because of limiting phosphate. The results presented here show that expression of APase is regulated both negatively and positively. PhoP, a homolog of a family of bacterial transcription factors, and PhoR, a homolog of bacterial histidine pr...

journal_title：Journal of bacteriology

authors： Jensen KK,Sharkova E,Duggan MF,Qi Y,Koide A,Hoch JA,Hulett FM

更新日期：1993-06-01 00:00:00

abstract：:A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its K(m) values were 2.4 x 10(-4) M d-glucosamine 6-phosphate and 0.38 x 10(-4) M Na(3)S-acetyl coenzyme A. ...

journal_title：Journal of bacteriology

authors： Giddings TH,Cantino EC

更新日期：1974-11-01 00:00:00

abstract：:The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic path...

journal_title：Journal of bacteriology

authors： Kelln RA,Kinahan JJ,Foltermann KF,O'Donovan GA

更新日期：1975-11-01 00:00:00

abstract：:When titanium(III) citrate was used as electron donor for the reduction of methyl coenzyme M by the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum delta H, component A1 was no longer required. The simpler system thus obtained required components A2, A3, and C as well as catalytic amou...

journal_title：Journal of bacteriology

authors： Rouvière PE,Bobik TA,Wolfe RS

更新日期：1988-09-01 00:00:00

abstract：:GTP cyclohydrolase II catalyzes the first committed step in the biosynthesis of riboflavin. The gene coding for this enzyme in Escherichia coli has been cloned by marker rescue. Sequencing indicated an open reading frame of 588 bp coding for a 21.8-kDa peptide of 196 amino acids. The gene was mapped to a position at 2...

journal_title：Journal of bacteriology

authors： Richter G,Ritz H,Katzenmeier G,Volk R,Kohnle A,Lottspeich F,Allendorf D,Bacher A

更新日期：1993-07-01 00:00:00

abstract：:The kinetics of beta-lactamase induction in Pseudomonas maltophilia IID1275/873 were investigated. Upon induction with beta-lactam antibiotics, a correlation was seen between the increase in specific beta-lactamase activity and the generation time, as well as the concentration of inducer in the medium. The specific be...

journal_title：Journal of bacteriology

authors： Rosta S,Mett H

更新日期：1989-01-01 00:00:00

abstract：:The WO-1 strain of Candida albicans is capable of alternating between two highly distinct yeast cell types termed white and opaque (E. H. A. Rikkerrink, B. B. Magee, and P. T. Magee, J. Bacteriol. 170:895-899, 1988; B. Slutsky, M. Staebell, J. Anderson, L. Risen, M. Pfaller, and D. R. Soll, J. Bacteriol. 169:189-197, ...

journal_title：Journal of bacteriology

authors： McEachern MJ,Hicks JB

更新日期：1991-12-01 00:00:00

abstract：:The mutant am-14 produces no active nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase (GDH) and no protein showing immunological cross-reaction with the enzyme. Nevertheless, it shows complementation with several other am mutants in heterokaryons. Active GDH can be extracted from heterokaryons...

journal_title：Journal of bacteriology

authors： Sundaram TK,Fincham JR

更新日期：1968-03-01 00:00:00

abstract：:Small nuclear ribonucleoprotein (snRNP) particles play an important role in the processing of pre-mRNA. snRNPs have been identified immunologically in a variety of cells, but none have ever been observed in prokaryotic systems. This report provides the first evidence for the presence of snRNP-like components in two ty...

journal_title：Journal of bacteriology

authors： Kovacs SA,O'Neil J,Watcharapijarn J,Moe-Kirvan C,Vijay S,Silva V

更新日期：1993-04-01 00:00:00

abstract：:Strains of Caulobacter crescentus elaborate an S-layer, a two-dimensional protein latticework which covers the cell surface. The S-layer protein (RsaA) is secreted by a type I mechanism (relying on a C-terminal signal) and is unusual among type I secreted proteins because high levels of protein are produced continuous...

journal_title：Journal of bacteriology

authors： Umelo-Njaka E,Bingle WH,Borchani F,Le KD,Awram P,Blake T,Nomellini JF,Smit J

更新日期：2002-05-01 00:00:00

abstract：:Lactococcus lactis subsp. lactis ML3 contains high pools of proline or betaine when grown under conditions of high osmotic strength. These pools are created by specific transport systems. A high-affinity uptake system for glycine betaine (betaine) with a Km of 1.5 microM is expressed constitutively. The activity of th...

journal_title：Journal of bacteriology

authors： Molenaar D,Hagting A,Alkema H,Driessen AJ,Konings WN

更新日期：1993-09-01 00:00:00

abstract：:The roles of three TATA binding protein (TBP) homologs (TBP1, TBP2, and TBP3) in the archaeon Methanosarcina acetivorans were investigated by using genetic and molecular approaches. Although tbp2 and tbp3 deletion mutants were readily obtained, a tbp1 mutant was not obtained, and the growth of a conditional tbp1 expre...

journal_title：Journal of bacteriology

authors： Reichlen MJ,Murakami KS,Ferry JG

更新日期：2010-03-01 00:00:00

abstract：:The highly purified lipoprotein of the outer membrane of Escherichia coli forms paracrystals. The ultrastructures of these paracrystals were examined by electron microscopy. The needle-shaped paracrystals show several different band patterns, depending on conditions of paracrystallization. Models are presented to expl...

journal_title：Journal of bacteriology

authors： DeMartini M,Inouye S,Inouye M

更新日期：1976-07-01 00:00:00

abstract：:Vibrio cholerae, the causative agent of cholera, has a sheathed, polar flagellum, and motility has been linked to virulence. An operon with two genes, flgO and flgP (VC2207 and VC2206), is positively regulated by FlrC, the activator of class III flagellar genes. Deletion of flgP results in a nonmotile phenotype, demon...

journal_title：Journal of bacteriology

authors： Morris DC,Peng F,Barker JR,Klose KE

更新日期：2008-01-01 00:00:00

abstract：:A class of galactose-resistant mutants has been derived from strains of Salmonella typhimurium which are defective in uridine diphosphoglucose-4-epimerase. Resistant strains are phenotypically similar to parent organisms but do not lyse in the presence of galactose. Low levels of functional epimerase can be detected i...

journal_title：Journal of bacteriology

authors： Shuster CW,Rundell K

更新日期：1969-10-01 00:00:00

abstract：:To examine as randomly as possible the role of the beta-ketoacyl and acyl carrier protein (ACP) components of bacterial type II polyketide synthases (PKSs), homologs of the chain-length-factor (CLF) genes were cloned from the environmental community of microorganisms. With PCR primers derived from conserved regions of...

journal_title：Journal of bacteriology

authors： Seow KT,Meurer G,Gerlitz M,Wendt-Pienkowski E,Hutchinson CR,Davies J

更新日期：1997-12-01 00:00:00