Abstract:
:When titanium(III) citrate was used as electron donor for the reduction of methyl coenzyme M by the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum delta H, component A1 was no longer required. The simpler system thus obtained required components A2, A3, and C as well as catalytic amounts of ATP, vitamin B12, and the disulfide of 7-mercaptoheptanoylthreonine phosphate in addition to titanium(III) citrate. This three component enzyme system also could produce CH4 when stoichiometric amounts of 7-mercaptoheptanoylthreonine phosphate were used as a source of electrons under an H2 atmosphere. When 7-mercaptoheptanoylthreonine phosphate or H2 was used alone no CH4 was produced, indicating a dual requirement for reducing equivalents: one to activate the methylreductase system and the other to reduce methyl coenzyme M. This is the first evidence that the activation of methyl coenzyme M methylreductase is a reductive process.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Rouvière PE,Bobik TA,Wolfe RSdoi
10.1128/jb.170.9.3946-3952.1988subject
Has Abstractpub_date
1988-09-01 00:00:00pages
3946-52issue
9eissn
0021-9193issn
1098-5530journal_volume
170pub_type
杂志文章abstract::The pattern of transcription of the rplKAJLrpoBC gene cluster of Escherichia coli appears to be complex. At least four different promoters and a transcriptional attenuator have been identified. To compare the relative effect of each of the putative promoters and the attenuator on transcription of these genes, we fused...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.279-285.1984
更新日期:1984-04-01 00:00:00
abstract::Clostridium perfringens causes gas gangrene and gastrointestinal (GI) diseases in humans. The most common cause of C. perfringens-associated food poisoning is the consumption of C. perfringens vegetative cells followed by sporulation and production of enterotoxin in the gut. Despite the importance of spore formation i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02152-12
更新日期:2013-06-01 00:00:00
abstract::Billen, Daniel (University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Tex.), and Roger Hewitt. Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication. J. Bacteriol. 92:609-617. 1966.-A study has been made of the subsequent replicative ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.3.609-617.1966
更新日期:1966-09-01 00:00:00
abstract::DNA sequence analysis of a region of the Streptomyces sp. strain C5 daunomycin biosynthesis gene cluster, located between the daunomycin polyketide biosynthesis gene cluster and a dnrI (transcriptional activator) homolog, revealed the presence of a gene encoding a P-450-like enzyme with a deduced Mr of 46,096. Express...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.11.3389-3395.1996
更新日期:1996-06-01 00:00:00
abstract::The bacterial peptidoglycan consists of glycan chains of repeating beta-1,4-linked N-acetylglucosaminyl-N-acetylmuramyl units cross-linked through short peptide chains. The polymerization of the glycans, or glycosyltransfer (GT), and transpeptidation (TP) are catalyzed by bifunctional penicillin-binding proteins (PBPs...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.15.4418-4423.2003
更新日期:2003-08-01 00:00:00
abstract::We analyzed expression of a putative oligopeptide permease (Opp) of Borrelia burgdorferi. Unlike the opp operons of other bacteria for which there is a single substrate binding protein, B. burgdorferi codes for three substrate binding proteins (OppA-I to -III) in its opp operon and an additional two homologs on plasmi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.22.6198-6206.2002
更新日期:2002-11-01 00:00:00
abstract::As part of an analysis of the conjugative transfer genes associated with the expression of F pili by plasmid F, we have investigated the physical location of the traC and traW genes. We found that plasmid clones carrying a 2.95-kilobase EcoRI-EcoRV F transfer operon fragment were able to complement transfer of F lac t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.11.5119-5124.1987
更新日期:1987-11-01 00:00:00
abstract::Many bacterial species produce capsular polysaccharides that contribute to pathogenesis through evasion of the host innate immune system. The gram-positive pathogen Enterococcus faecalis was previously reported to produce one of four capsule serotypes (A, B, C, or D). Previous studies describing the four capsule serot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00592-09
更新日期:2009-10-01 00:00:00
abstract::As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5579-5585.1996
更新日期:1996-10-01 00:00:00
abstract::The sugar composition of lipopolysaccharide (LPS) isolated from whole cells of Alteromonas haloplanktis 214 (previously referred to as marine pseudomonas B-16, ATCC 19855), variant 3, of the lipid A, core, and side-chain fractions derived from it, and of the LPS fractions (LPS I, II, and III) obtained by subjecting it...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.158-167.1978
更新日期:1978-10-01 00:00:00
abstract::We previously elucidated the global transcriptional responses of Escherichia coli to the nitrosating agent S-nitrosoglutathione (GSNO) in both aerobic and anaerobic chemostats, demonstrated the expression of nitric oxide (NO)-protective mechanisms, and obtained evidence of critical thiol nitrosation. The present study...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01354-06
更新日期:2007-03-01 00:00:00
abstract::DNA uptake by natural competence is a central process underlying the genetic plasticity, biology, and virulence of the human respiratory opportunistic pathogen Streptococcus pneumoniae A study reported in this issue (J. Slager, R. Aprianto, and J.-W. Veening, J. Bacteriol. 201:e00780-18, https://doi.org/10.1128/JB.007...
journal_title:Journal of bacteriology
pub_type: 评论,杂志文章
doi:10.1128/JB.00238-19
更新日期:2019-06-10 00:00:00
abstract::A chemotaxis system for Pseudomonas aeruginosa was defined by using the method of Adler. Cells were attracted to compounds in the order ammonium chloride greater than amino acids greater than organic acids. Two sugars were assayed and elicited no response. Comparisons with other model systems are discussed. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.427-429.1978
更新日期:1978-01-01 00:00:00
abstract::The level of extracellular alpha-amylase (EC 3.2.1.1) of Bacillus subtilis Marburg was increased about fivefold by introducing the amyR marker from B. natto 1212 through transformation. amyR2 of B. natto 1212 has been assumed to determine a high level of alpha-amylase of the organism. The gene acts specifically on alp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.410-415.1974
更新日期:1974-08-01 00:00:00
abstract::A specific gamma-aminobutyrate (GABA) transport system in Escherichia coli K-12 cells with a K(m) of 12 muM and a V(max) of 278 nmol/ml of intracellular water per min is described. Membrane vesicles contained d-lactate-dependent activity of the system. Mutants defective in GABA transport were isolated; they lost the a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.2.295-299.1978
更新日期:1978-08-01 00:00:00
abstract::Biodegradation of metal-citrate complexes by Pseudomonas fluorescens depends on the nature of the complex formed between the metal and citric acid. Bidentate Fe(III)-, Ni-, and Zn-citrate complexes were readily biodegraded, but the tridentate Cd- and Cu-citrate, and U-citrate complexes were not. The biodegradation of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.8.1989-1993.1995
更新日期:1995-04-01 00:00:00
abstract::Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3634-3640.1989
更新日期:1989-07-01 00:00:00
abstract::A pentachlorophenol (PCP) hydroxylase which catalyzed the conversion of PCP to 2,3,5,6-tetrachlorohydroquinone and released iodide from triiodophenol in the presence of NADPH and oxygen was identified. The enzyme was purified by protamine sulfate precipitation, ammonium sulfate precipitation, hydrophobic chromatograph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.14.4447-4453.1991
更新日期:1991-07-01 00:00:00
abstract::The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01532-08
更新日期:2009-03-01 00:00:00
abstract::A procedure for protoplast formation in Escherichia coli is described. Removal of the cell wall was confirmed by examination of cells in thin-section preparations. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.2.668-670.1976
更新日期:1976-11-01 00:00:00
abstract::Gram-negative bacteria acquire ferric siderophores through TonB-dependent outer membrane transporters (TBDT). By fluorescence spectroscopic hgh-throughput screening (FLHTS), we identified inhibitors of TonB-dependent ferric enterobactin (FeEnt) uptake through Escherichia coli FepA (EcoFepA). Among 165 inhibitors found...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00889-16
更新日期:2017-04-25 00:00:00
abstract::Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential expression explains the establishment of hsdK genes in a nonmodified...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4905-4906.1993
更新日期:1993-08-01 00:00:00
abstract::The structure and composition of the cell walls of hyphae of Neurospora crassa were investigated by electron microscopy, chemical analysis, and X-ray diffraction both before and after progressive enzymatic degradation by snail gut enzymes, chitinase, and trypsin. The wall consists of two phases: randomly disposed skel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.202-212.1967
更新日期:1967-07-01 00:00:00
abstract::This is the first complete genome sequence of the Enterobacter aerogenes species. Here we present the genome sequence of E. aerogenes KCTC 2190, which contains 5,280,350 bp with a G + C content of 54.8 mol%, 4,912 protein-coding genes, and 109 structural RNAs. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00028-12
更新日期:2012-05-01 00:00:00
abstract::Bacillus brevis HPD31 contains a surface (S)-layer protein, termed the HWP, which forms a hexagonal array in the cell wall. The 5' region of the HWP gene was isolated from a DNA library constructed in bacteriophage vector EMBL3 from a partial BamHI digest of the chromosomal DNA. The 3' region contained in a 2.7-kiloba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1312-1320.1990
更新日期:1990-03-01 00:00:00
abstract::The hemA mutation of Escherichia coli K-12 confers a requirement for delta-aminolevulinic acid (ALA). Cell extract prepared from the hemA strain SASX41B was incapable of producing ALA from either glutamate or glutamyl-tRNA, whereas extract of the hem+ strain HB101 formed colorimetrically detectable amounts of ALA and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.2919-2924.1989
更新日期:1989-06-01 00:00:00
abstract::The Mycobacterium tuberculosis gene Rv2747 encodes a novel 19-kDa ArgA that catalyzes the initial step in L-arginine biosynthesis, namely the conversion of L-glutamate to alpha-N-acetyl-L-glutamate. Initial velocity studies reveal that Rv2747 proceeds through a sequential kinetic mechanism, with K(m) values of 280 mM ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.9.3039-3044.2005
更新日期:2005-05-01 00:00:00
abstract::Plasmid pP51 of Pseudomonas sp. strain P51 contains two gene clusters encoding the degradation of chlorinated benzenes, tcbAB and tcbCDEF. A regulatory gene, tcbR, was located upstream and divergently transcribed from the chlorocatechol oxidative gene cluster tcbCDEF. The tcbR gene was characterized by DNA sequencing ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.12.3700-3708.1991
更新日期:1991-06-01 00:00:00
abstract::Reference strains from 48 selected serovars representing eight species of Leptospira were examined by two polymerase chain reaction (PCR)-based strategies. First, mapped restriction site polymorphisms (MRSP) were examined in PCR products from portions of rrs (16S rRNA gene) and rrl (23S rRNA gene). Twenty MRSP and 2 l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.4.973-981.1993
更新日期:1993-02-01 00:00:00
abstract::Goldberg, Herbert S. (University of Missouri, Columbia), Ella M. Barnes, and Anthony B. Charles. Unusual Bacteroides-like organism. J. Bacteriol. 87:737-742. 1964.-An organism is described which appears to be a new species of gram-negative, anaerobic, nonsporulating rod. It was isolated from poultry caeca at levels of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.3.737-742.1964
更新日期:1964-03-01 00:00:00