Abstract:
:The sugar composition of lipopolysaccharide (LPS) isolated from whole cells of Alteromonas haloplanktis 214 (previously referred to as marine pseudomonas B-16, ATCC 19855), variant 3, of the lipid A, core, and side-chain fractions derived from it, and of the LPS fractions (LPS I, II, and III) obtained by subjecting it to preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis has been determined. Conditions optimum for the release of constituent monosaccharides by hydrolysis were established. Sugars were quantitated by gas-liquid chromatography of their alditol acetate derivatives. Lipid A was detected by gel electrophoresis and by the spectral shift obtained with a carbocyanin dye. A comparison of the molar ratios of the various fractions suggest that LPS III is an LPS molecule lacking an O-antigenic side chain, whereas LPS I and II are LPS molecules differing in side-chain composition. LPS I may be a mixture of two LPS species. In double immunodiffusion experiments using anti-whole-cell serum, LPS I and II showed a homologous cross-reaction with isolated whole-cell LPS. LPS III as well as lipid A, core, and side-chain fractions failed to give rise to precipitin lines.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
DiRienzo JM,MacLeod RAdoi
10.1128/JB.136.1.158-167.1978keywords:
subject
Has Abstractpub_date
1978-10-01 00:00:00pages
158-67issue
1eissn
0021-9193issn
1098-5530journal_volume
136pub_type
杂志文章abstract::Temperate bacteriophages effect chromosomal evolution of their bacterial hosts, mediating rearrangements and the acquisition of novel genes from other taxa. Although the Haemophilus influenzae genome shows evidence of past phage-mediated lateral transfer, the phages presumed responsible have not been identified. To da...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.24.6893-6905.2002
更新日期:2002-12-01 00:00:00
abstract::The reactivation of membranes from Gaffkya homari for the synthesis of sodium dodecyl sulfate-insoluble peptidoglycan (SDS-insoluble PG) was achieved by successive cycles of freeze-thawing (- 196 versus 25 degrees C). The presence of G. homari walls during this process affected the synthesis of both SDS-soluble (nasce...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.535-544.1982
更新日期:1982-05-01 00:00:00
abstract::Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01008-10
更新日期:2011-01-01 00:00:00
abstract::Synthesis of heme, measured by incorporation of iron-59, and of bacteriochlorophyll was studied with wild-type and mutant strains of Rhodopseudomonas spheroides. The wild type formed heme from glycine and succinate at one-fortieth the rate of bacteriochlorophyll under anaerobic-light conditions. Added delta-aminolevul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.2.712-720.1969
更新日期:1969-05-01 00:00:00
abstract::Quorum sensing of Sinorhizobium meliloti relies on N-acyl-homoserine lactones (AHLs) as autoinducers. AHL production increases at high population density, and this depends on the AHL synthase SinI and two transcriptional regulators, SinR and ExpR. Our study demonstrates that ectopic expression of the gene rne, coding ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01471-13
更新日期:2014-04-01 00:00:00
abstract::During the progression of Mycoplasma hominis cultures from the early logarithmic phase to the stationary phase of growth, the total phospholipid content of the cell membranes decreased. Measurement of the amount of the various phospholipids during the growth cycle showed that a decrease in the phosphatidylglycerol (PG...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.631-639.1975
更新日期:1975-02-01 00:00:00
abstract::Shafia, Fred (University of Nebraska, Lincoln), and T. L. Thompson. Isolation and preliminary characterization of bacteriophage phimu-4. J. Bacteriol. 87:999-1002. 1964.-Bacteriophage phimu-4 was isolated from lysogenic Bacillus stearothermophilus NU strain 4, and was propagated in strain 10 of the same species. The p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.999-1002.1964
更新日期:1964-05-01 00:00:00
abstract::Replication of the broad-host-range, IncPalpha plasmid RK2 requires two plasmid loci: trfA, the replication initiator gene, and oriV, the origin of replication. While these determinants are sufficient for replication in a wide variety of bacteria, they do not confer the stable maintenance of parental RK2 observed in i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.21.6014-6026.2000
更新日期:2000-11-01 00:00:00
abstract::The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00876-13
更新日期:2013-10-01 00:00:00
abstract::The extracellular protease of Pseudomonas maltophilia was partially purified by ammonium sulfate precipitation and chromatography on Sephadex G-75 and Bio-rex 70. Gel electrophoresis revealed minor impurities. The enzyme exhibited the following properties: (i) molecular weight, 35,000; (ii) A see article; 10.8; (iii) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.3.933-941.1975
更新日期:1975-03-01 00:00:00
abstract::Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.1.262-265.1972
更新日期:1972-01-01 00:00:00
abstract::The effect of the iron content of the medium on the yields of extracellular products by seven distinct strains of Pseudomonas aeruginosa was examined. All strains showed at least an 85% decrease in toxin A yields when grown in medium containing 5.0 mug of iron per ml (high iron) as compared to 0.05 mug/ml (low iron), ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.193-200.1979
更新日期:1979-04-01 00:00:00
abstract::Langridge, Patricia (Oregon State University, Corvallis), and Richard Y. Morita. Thermolability of malic dehydrogenase from the obligate psychrophile Vibrio marinus. J. Bacteriol. 92:418-423. 1966.-The thermolability of malic dehydrogenase in whole cells of Vibrio marinus MP-1 grown at 15 C was compared with that of c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.2.418-423.1966
更新日期:1966-08-01 00:00:00
abstract::Based on its genome sequence, the pathway of beta-oxidative fatty acid degradation in Salmonella enterica serovar Typhimurium LT2 has been thought to be identical to the well-characterized Escherichia coli K-12 system. We report that wild-type strains of S. enterica grow on decanoic acid, whereas wild-type E. coli str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.2.599-608.2006
更新日期:2006-01-01 00:00:00
abstract::The Bacillus subtilis skin element confers resistance to arsenate and arsenite. The ars operon in the skin element contains four genes in the order arsR, ORF2, arsB, and arsC. Three of these genes are homologous to the arsR, arsB, and arsC genes from the staphylococcal plasmid pI258, while no homologs of ORF2 have bee...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.7.1655-1661.1998
更新日期:1998-04-01 00:00:00
abstract::The umuDC gene products, whose expression is induced by DNA-damaging treatments, have been extensively characterized for their role in SOS mutagenesis. We have recently presented evidence that supports a role for the umuDC gene products in the regulation of growth after DNA damage in exponentially growing cells, analo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.4.1127-1135.2000
更新日期:2000-02-01 00:00:00
abstract::The tsr gene specifies a methyl-accepting membrane protein involved in chemotaxis to serine and several repellent compounds. We have characterized a special class of tsr mutations designated cheD which alter the signaling properties of the Tsr transducer. Unlike tsr null mutants, cheD strains are generally nonchemotac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.96-104.1985
更新日期:1985-01-01 00:00:00
abstract::When the stop codons TGA, TAA, and TAG are found in the second and third reading frames of a protein-encoding gene, they are considered premature stop codons (PSC). Deinococcus radiodurans disproportionately favored TGA more than the other two triplets as a PSC. The TGA triplet was also found more often in noncoding r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00682-08
更新日期:2008-10-01 00:00:00
abstract::Bergdoll, Merlin S. (University of Chicago, Chicago, Ill.), Concordia R. Borja, and Remedios M. Avena. Identification of a new enterotoxin as enterotoxin C. J. Bacteriol. 90:1481-1485. 1965.-Identification of a new enterotoxin was accomplished by purification of the enterotoxins produced by staphylococcal strains 137 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.5.1481-1485.1965
更新日期:1965-11-01 00:00:00
abstract::Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.565-567.1974
更新日期:1974-10-01 00:00:00
abstract::Among strains of Haemophilus influenzae, the ability to catabolize tryptophan (as detected by indole production) varies and is correlated with pathogenicity. Tryptophan catabolism is widespread (70 to 75%) among harmless respiratory isolates but is nearly universal (94 to 100%) among strains causing serious disease, i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.1.107-118.1998
更新日期:1998-01-01 00:00:00
abstract::Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The pa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.425-427.1980
更新日期:1980-10-01 00:00:00
abstract::Acidithiobacillus caldus is an extremely acidophilic, moderately thermophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and reduced inorganic sulfur compounds. Here we present the draft genome sequence of Acidithiobacillus caldus ATCC 51756 (the type strain of the spe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00843-09
更新日期:2009-09-01 00:00:00
abstract::To investigate the function of spot 42 RNA, a small RNA of Escherichia coli, we constructed a strain in which spf, the structural gene for this RNA, is deleted. We achieved this by using a delta att phage lambda carrying a DNA fragment spanning the spf region but with a precise deletion of spf. By integration of this ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.746-750.1986
更新日期:1986-06-01 00:00:00
abstract::Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.3.601-611.1997
更新日期:1997-02-01 00:00:00
abstract::In Shigella flexneri, the polysaccharide copolymerase (PCP) protein Wzz(SF) confers a modal length of 10 to 17 repeat units (RUs) to the O-antigen (Oag) component of lipopolysaccharide (LPS). PCPs form oligomeric structures believed to be related to their function. To identify functionally important regions within Wzz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01134-09
更新日期:2010-07-01 00:00:00
abstract::Bacteriocins are ribosomally synthesized proteinaceous antibacterial peptides. They selectively interfere with the growth of other bacteria. The production and secretion of bacteriocins confer a distinct ecological advantage to the producer in competing against other bacteria that are present in the same ecological ni...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00762-19
更新日期:2020-05-27 00:00:00
abstract::The function of the long propeptides of fungal proteinases is not known. Aspergillus fumigatus produces a 33-kDa serine proteinase of the subtilisin family and a 42-kDa metalloproteinase of the thermolysin family. These extracellular enzymes are synthesized as preproenzymes containing large amino-terminal propeptides....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2211-2215.1996
更新日期:1996-04-01 00:00:00
abstract::The glutamate permeation system in Escherichia coli K-12 consists of three genes: gltC, gltS, and gltR. The genes gltC and gltS are very closely linked, and are located between the pyrE and tna loci, in the following order: tna, gltC, gltS, pyrE; gltR is located near the metA gene. The three glt genes constitute a reg...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1118-1128.1969
更新日期:1969-03-01 00:00:00
abstract::Autoinducer-2 (AI-2) is required for biofilm formation and virulence of the oral pathogen Aggregatibacter actinomycetemcomitans, and we previously showed that lsrB codes for a receptor for AI-2. The lsrB gene is expressed as part of the lsrACDBFG operon, which is divergently transcribed from an adjacent lsrRK operon. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01476-12
更新日期:2013-01-01 00:00:00