Abstract:
:The umuDC gene products, whose expression is induced by DNA-damaging treatments, have been extensively characterized for their role in SOS mutagenesis. We have recently presented evidence that supports a role for the umuDC gene products in the regulation of growth after DNA damage in exponentially growing cells, analogous to a prokaryotic DNA damage checkpoint. Our further characterization of the growth inhibition at 30 degrees C associated with constitutive expression of the umuDC gene products from a multicopy plasmid has shown that the umuDC gene products specifically inhibit the transition from stationary phase to exponential growth at the restrictive temperature of 30 degrees C and that this is correlated with a rapid inhibition of DNA synthesis. These observations led to the finding that physiologically relevant levels of the umuDC gene products, expressed from a single, SOS-regulated chromosomal copy of the operon, modulate the transition to rapid growth in E. coli cells that have experienced DNA damage while in stationary phase. This activity of the umuDC gene products is correlated with an increase in survival after UV irradiation. In a distinction from SOS mutagenesis, uncleaved UmuD together with UmuC is responsible for this activity. The umuDC-dependent increase in resistance in UV-irradiated stationary-phase cells appears to involve, at least in part, counteracting a Fis-dependent activity and thereby regulating the transition to rapid growth in cells that have experienced DNA damage. Thus, the umuDC gene products appear to increase DNA damage tolerance at least partially by regulating growth after DNA damage in both exponentially growing and stationary-phase cells.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Murli S,Opperman T,Smith BT,Walker GCdoi
10.1128/jb.182.4.1127-1135.2000keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
1127-35issue
4eissn
0021-9193issn
1098-5530journal_volume
182pub_type
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doi:10.1128/jb.174.1.303-308.1992
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journal_title:Journal of bacteriology
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更新日期:1978-01-01 00:00:00
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更新日期:1999-07-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1997-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.3.1096-1100.2001
更新日期:2001-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1975-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.1.33-38.1964
更新日期:1964-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.1019-1024.1980
更新日期:1980-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.165-169.1976
更新日期:1976-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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更新日期:2019-10-21 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.9.3042-3048.1992
更新日期:1992-05-01 00:00:00
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更新日期:1998-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.93.2.577-579.1967
更新日期:1967-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.7.2416-2417.1992
更新日期:1992-04-01 00:00:00
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更新日期:2007-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1962-10-01 00:00:00
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更新日期:2014-10-01 00:00:00
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更新日期:2002-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1993-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1986-12-01 00:00:00
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更新日期:2004-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1998-02-01 00:00:00