Abstract:
:Structural analogs of S-adenosylhomocysteine were tested in vitro for inhibition of the yeast S-adenosylmethionine:delta 24-sterol-C-methyltransferase enzyme. A wide inhibitory range by these compounds was observed, suggesting which structural features of the parent compound are important for binding to the enzyme. No analog tested had inhibitory activity specific only for this enzyme. The most active compound was sinefungin, a metabolite of Streptomyces griseolus, which was also able to inhibit growth of yeast cultures. Sterol extracts of cells grown in the presence of sinefungin revealed a dramatic increase in the levels of zymosterol, the sterol substrate in the transmethylation under study, and a concomitant decrease in the levels of ergosterol. Evidence is presented that sinefungin is transported inside the cell by the same permease as S-adenosylmethionine. We conclude that sinefungin is blocking the in vivo methylation of sterols in yeast. The implications of this finding are discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
McCammon MT,Parks LWdoi
10.1128/JB.145.1.106-112.1981subject
Has Abstractpub_date
1981-01-01 00:00:00pages
106-12issue
1eissn
0021-9193issn
1098-5530journal_volume
145pub_type
杂志文章abstract::During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr(1068), in the evolutionarily hypervariable segment of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.10.3470-3476.2006
更新日期:2006-05-01 00:00:00
abstract::The minimal origin of replication of the broad-host-range plasmid RK2 has two potential recognition sequences for the DnaA protein of Escherichia coli. DNA transfer by transformation into a dnaA-null mutant of E. coli showed that DnaA protein is needed for replication or maintenance of mini-RK2. We isolated and purifi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4703-4709.1987
更新日期:1987-10-01 00:00:00
abstract::Teichoic acid-like material extracted by cold trichloroacetic acid from lyophilized whole cells of streptococci from groups A,D,E,O, and T was shown to give a positive precipitin reaction with group antisera. Similar material from cells of groups B,C,F,G,H,K,L,M,N,P,Q,R, and S did not give a positive reaction with gro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.3.747-752.1970
更新日期:1970-06-01 00:00:00
abstract::The temperature-sensitive cyr1-2 mutant in Saccharomyces cerevisiae produces low levels of adenylate cyclase and cyclic AMP at 25 degrees C and is unable to synthesize repressible acid phosphatase at 25 degrees C. Suppressor mutants of cyr1-2 were isolated by detecting acid phosphatase activity. One of the dominant su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.7.2406-2408.1991
更新日期:1991-04-01 00:00:00
abstract::An obligate thermophilic strain of Bacillus stearothermophilus, strain PH24, isolated from industrial sediment by elective culture, grew readily at 55 C on phenol or on one of the isomers of cresol as the major carbon source. Intact cells grown in the presence of phenol, o-cresol, m-cresol, or p-cresol were induced to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1077-1083.1975
更新日期:1975-12-01 00:00:00
abstract::Nishida, S. (Kanazawa University, Kanazawa, Japan), and G. Nakagawara. Isolation of toxigenic strains of Clostridium novyi from soil. J. Bacteriol. 88:1636-1640. 1964.-The cultural conditions for toxin production by Clostridium novyi were investigated, and the optimal constituents of a proper medium were determined. A...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.6.1636-1640.1964
更新日期:1964-12-01 00:00:00
abstract::The determinant of resistance to fosfomycin of the Serratia marcescens plasmid pOU900 was transposed into the plasmid ColE1 and into the plasmid RP4 in the absence of the RecA function of the host. In each case, the acquisition of fosfomycin resistance was correlated with the presence of a discrete piece of DNA, unifo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.477-479.1982
更新日期:1982-07-01 00:00:00
abstract::The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs. We have cloned and sequenced the leu genes from one auxotroph, IL1403. The sequence is 99% homologo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.14.4383-4390.1993
更新日期:1993-07-01 00:00:00
abstract::We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.14.4665-4684.2004
更新日期:2004-07-01 00:00:00
abstract::We examined the predacious gram-negative bacterium Bdellovibrio bacteriovorous 109J and free-living strains 109J-A1 and 109J-KA1 derived therefrom for penicillin-binding proteins (PBPs). We compared their PBPs with those of the host bacterium, Escherichia coli, and with those of a facultatively predacious bdellovibrio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3750-3751.1988
更新日期:1988-08-01 00:00:00
abstract::Four genes that encode the homologues of plant geranylgeranyl reductase were isolated from a hyperthermophilic archaeon Archaeoglobus fulgidus, which produces menaquinone with a fully saturated heptaprenyl side chain, menaquinone-7(14H). The recombinant expression of one of the homologues in Escherichia coli led to a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.6.1937-1944.2005
更新日期:2005-03-01 00:00:00
abstract::The SecYEG heterotrimeric membrane protein complex functions as a channel for protein translocation across the Escherichia coli cytoplasmic membrane. SecY is the central subunit of the SecYEG complex and contains 10 transmembrane segments (TM1 to TM10). Previous mutation studies suggested that TM3 and TM4 are particul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.12.3960-3969.2004
更新日期:2004-06-01 00:00:00
abstract::Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most gram-negative bacteria, and its structure and biosynthetic pathway are well known. Nevertheless, the mechanisms of transport and assembly of this molecule at the cell surface are poorly understood. The inner membrane (IM) transport p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00270-08
更新日期:2008-07-01 00:00:00
abstract::Antipain kills Escherichia coli K-12 cells in an exponential manner beginning 1 h after its addition. Mutant strains, delta cya and crp, which are unable to synthesize cyclic adenosine 3',5'-monophosphate (cAMP) and the cAMP receptor protein, respectively, are not affected. Addition of cAMP (5 mM) to antipain-treated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.690-693.1979
更新日期:1979-08-01 00:00:00
abstract::The ugp operon of Escherichia coli includes genes involved in the uptake of sn-glycerol-3-phosphate and glycerophosphoryl diesters and belongs to the pho regulon which is induced by phosphate limitation. This operon has two transcriptional initiation sites, as determined by S1 nuclease mapping of the in vivo transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.549-558.1991
更新日期:1991-01-01 00:00:00
abstract::From a lysogen with lambda integrated in the leu operon, specialized transducing phages that carry the cell division, murein biosynthesis, and envelope permeability genes located about 0.5 min to the right of leu were isolated. These phages were used to identify the previously undiscovered cell division gene sep. A ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.91-100.1978
更新日期:1978-01-01 00:00:00
abstract::Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.2.782-784.1975
更新日期:1975-05-01 00:00:00
abstract::Single-celled organisms must survive exposure to environmental extremes. Perhaps one of the most variable and potentially life-threatening changes that can occur is that of a rapid and acute decrease in external osmolarity. This easily translates into several atmospheres of additional pressure that can build up within...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00576-12
更新日期:2012-09-01 00:00:00
abstract::The chromosomally mediated penicillinase present in three strains of Escherichia coli K-12 has been purified and characterized. Two of the strains carried the ampA gene and the third the wild-type allele. The purification involves release of the enzyme by spheroplast formation, dialysis, chromatography on sulfoethyl c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.1.218-231.1970
更新日期:1970-01-01 00:00:00
abstract::The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4890-4896.1988
更新日期:1988-10-01 00:00:00
abstract::Picolinic acid (PA), a typical C-2-carboxylated pyridine derivative, is a metabolite of l-tryptophan and many other aromatic compounds in mammalian and microbial cells. Microorganisms can degrade and utilize PA for growth. However, the precise mechanism of PA metabolism remains unknown. Alcaligenes faecalis strain JQ1...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00665-18
更新日期:2019-03-13 00:00:00
abstract::PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F' strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.1.119-128.1972
更新日期:1972-07-01 00:00:00
abstract::The magnetosomes of many magnetotactic bacteria consist of membrane-enveloped magnetite crystals, whose synthesis is favored by a low redox potential. However, the cellular redox processes governing the biomineralization of the mixed-valence iron oxide have remained unknown. Here, we show that in the alphaproteobacter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00903-12
更新日期:2012-09-01 00:00:00
abstract::The TcbR transcriptional activator protein, which is encoded by the tcbR gene of Pseudomonas sp. strain P51 (J. R. van der Meer, A. C. J. Frijters, J. H. J. Leveau, R. I. L. Eggen, A. J. B. Zehnder, and W. M. de Vos, J. Bacteriol. 173:3700-3708, 1991), was purified from overproducing Escherichia coli cells by using a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.1850-1856.1994
更新日期:1994-04-01 00:00:00
abstract::Plasmid R1 is a low-copy-number plasmid that is present at a level of about four or five copies per average cell. The copy number is controlled posttranscriptionally at the level of synthesis of the rate-limiting initiator protein RepA. In addition to this, R1 has an auxiliary system that derepresses a second promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.1.207-211.2004
更新日期:2004-01-01 00:00:00
abstract::The histidine ATP-binding cassette (ABC) transporter of Salmonella enterica serovar Typhimurium is among the best-studied type I ABC import systems. The transporter consists of two transmembrane subunits, HisQ and HisM, and a homodimer of the nucleotide-binding subunit, HisP. Substrates are delivered by two periplasmi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00521-18
更新日期:2018-12-20 00:00:00
abstract::We studied the activity of a debranching enzyme (TreX) from Sulfolobus solfataricus on glycogen-mimic substrates, branched maltotetraosyl-β-cyclodextrin (Glc₄-β-CD), and natural glycogen to better understand substrate transglycosylation and the effect thereof on glycogen debranching in microorganisms. The validation t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01442-13
更新日期:2014-06-01 00:00:00
abstract::The phosphomannose isomerase (pmi) gene of Escherichia coli was cloned on a broad-host-range cosmid vector and expressed in Pseudomonas aeruginosa at a low level. Plasmid pAD3, which harbors the E. coli pmi gene, contains a 6.2-kilobase-pair HindIII fragment derived from the chromosome of E. coli. Subcloning produced ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.249-257.1985
更新日期:1985-01-01 00:00:00
abstract::Tn4551, a clindamycin resistance (Ccr) transposon from the R plasmid pBI136, was cloned onto an Escherichia coli-Bacteroides shuttle vector which could replicate normally in E. coli but was maintained unstably in Bacteroides fragilis. To aid in cloning and to ensure maintenance of Tn4551 in E. coli, a kanamycin resist...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3450-3457.1987
更新日期:1987-08-01 00:00:00
abstract::Sbarra, Anthony J. (St. Margaret's Hospital, Boston, Mass.) and William Shirley. Phagocytosis inhibition and reversal. I. Effect of glycolytic intermediates and nucleotides on particle uptake. J. Bacteriol. 86:259-265. 1963.-By microscopically monitoring phagocytosis and following the biochemical changes associated wi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.2.259-265.1963
更新日期:1963-08-01 00:00:00