The periplasmic nitrate reductase nap is required for anaerobic growth and involved in redox control of magnetite biomineralization in Magnetospirillum gryphiswaldense.

Abstract:

:The magnetosomes of many magnetotactic bacteria consist of membrane-enveloped magnetite crystals, whose synthesis is favored by a low redox potential. However, the cellular redox processes governing the biomineralization of the mixed-valence iron oxide have remained unknown. Here, we show that in the alphaproteobacterium Magnetospirillum gryphiswaldense, magnetite biomineralization is linked to dissimilatory nitrate reduction. A complete denitrification pathway, including gene functions for nitrate (nap), nitrite (nir), nitric oxide (nor), and nitrous oxide reduction (nos), was identified. Transcriptional gusA fusions as reporters revealed that except for nap, the highest expression of the denitrification genes coincided with conditions permitting maximum magnetite synthesis. Whereas microaerobic denitrification overlapped with oxygen respiration, nitrate was the only electron acceptor supporting growth in the entire absence of oxygen, and only the deletion of nap genes, encoding a periplasmic nitrate reductase, and not deletion of nor or nos genes, abolished anaerobic growth and also delayed aerobic growth in both nitrate and ammonium media. While loss of nosZ or norCB had no or relatively weak effects on magnetosome synthesis, deletion of nap severely impaired magnetite biomineralization and resulted in fewer, smaller, and irregular crystals during denitrification and also microaerobic respiration, probably by disturbing the proper redox balance required for magnetite synthesis. In contrast to the case for the wild type, biomineralization in Δnap cells was independent of the oxidation state of carbon substrates. Altogether, our data demonstrate that in addition to its essential role in anaerobic respiration, the periplasmic nitrate reductase Nap has a further key function by participating in redox reactions required for magnetite biomineralization.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Li Y,Katzmann E,Borg S,Schüler D

doi

10.1128/JB.00903-12

subject

Has Abstract

pub_date

2012-09-01 00:00:00

pages

4847-56

issue

18

eissn

0021-9193

issn

1098-5530

pii

JB.00903-12

journal_volume

194

pub_type

杂志文章
  • Sucrose catabolism in Clostridium pasteurianum and its relation to N2 fixation.

    abstract::The growth constant and Y (sucrose) (grams of cells per mole of sucrose) for NH(3)-grown cultures of Clostridium pasteurianum were 1.7 times those of N(2)-grown cultures, whereas the rate of sucrose utilized per gram of cells per hour was similar for both conditions. The Y (sucrose) of chemostat cultures grown on limi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.2.346-351.1968

    authors: Daesch G,Mortenson LE

    更新日期:1968-08-01 00:00:00

  • Subcellular localization and immunological detection of proteins encoded by the vir locus of Bordetella pertussis.

    abstract::The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.14.4288-4296.1991

    authors: Stibitz S,Yang MS

    更新日期:1991-07-01 00:00:00

  • Leptospira species categorized by arbitrarily primed polymerase chain reaction (PCR) and by mapped restriction polymorphisms in PCR-amplified rRNA genes.

    abstract::Reference strains from 48 selected serovars representing eight species of Leptospira were examined by two polymerase chain reaction (PCR)-based strategies. First, mapped restriction site polymorphisms (MRSP) were examined in PCR products from portions of rrs (16S rRNA gene) and rrl (23S rRNA gene). Twenty MRSP and 2 l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.4.973-981.1993

    authors: Ralph D,McClelland M,Welsh J,Baranton G,Perolat P

    更新日期:1993-02-01 00:00:00

  • Isolation of the lipid intermediate in peptidoglycan biosynthesis from Escherichia coli.

    abstract::The lipid intermediate in peptidoglycan biosynthesis was isolated from Escherichia coli strain W and characterized as C(55)-isoprenyl-pyrophosphoryl N-acetylmuramyl(-pentapeptide)-N-acetyl-glucosamine. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1306-1309.1972

    authors: Umbreit JN,Strominger JL

    更新日期:1972-12-01 00:00:00

  • Electron microscopy of single-stranded structures in the DNA of competent Haemophilus influenzae cells.

    abstract::Chromosomal DNAs from exponential-phase and competent cells of Haemophilus influenzae were examined by electron microscopy to determine whether the chromosome undergoes structural changes during competence development. Single-stranded gaps and single-stranded tails formed in chromosomal DNA during competence developme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.565-571.1987

    authors: McCarthy D,Kupfer DM

    更新日期:1987-02-01 00:00:00

  • Structural differences in the catalytic subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides.

    abstract::There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.3.1188-1193.1985

    authors: Gibson JL,Tabita FR

    更新日期:1985-12-01 00:00:00

  • Sequence and distribution of IS1312: evidence for horizontal DNA transfer from Rhizobium meliloti to Agrobacterium tumefaciens.

    abstract::Two novel insertion sequences, IS1312 and IS1313, were found in pTiBo542, the Ti plasmid of Agrobacterium tumefaciens strains Bo542 and A281. Nucleotide sequencing and Southern hybridization revealed that IS1312 and IS1313 are homologous to Rhizobium meliloti ISRm1 and ISRm2, respectively. IS1312, ISRm1, and another A...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.9.2554-2559.1995

    authors: Deng W,Gordon MP,Nester EW

    更新日期:1995-05-01 00:00:00

  • The iron-regulated iupABC operon is required for saprophytic growth of the intracellular pathogen Rhodococcus equi at low iron concentrations.

    abstract::Rhodococcus equi is a facultative intracellular pathogen which proliferates rapidly in both manure-enriched soil and alveolar macrophages. Although both environments are characterized by extremely low concentrations of free iron, very little is known regarding the strategies employed by R. equi to thrive under these c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.10.3438-3444.2005

    authors: Miranda-Casoluengo R,Duffy PS,O'Connell EP,Graham BJ,Mangan MW,Prescott JF,Meijer WG

    更新日期:2005-05-01 00:00:00

  • Major role of NAD-dependent lactate dehydrogenases in aerobic lactate utilization in Lactobacillus plantarum during early stationary phase.

    abstract::NAD-independent lactate dehydrogenases are commonly thought to be responsible for lactate utilization during the stationary phase of aerobic growth in Lactobacillus plantarum. To substantiate this view, we constructed single and double knockout mutants for the corresponding genes, loxD and loxL. Lactate-to-acetate con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.19.6661-6666.2004

    authors: Goffin P,Lorquet F,Kleerebezem M,Hols P

    更新日期:2004-10-01 00:00:00

  • Molecular characterization of "plasmid-free" antibiotic-resistant Haemophilus influenzae.

    abstract::We examined 14 multiresistant and 8 ampicillin- or tetracycline-resistant Haemophilus influenzae isolates and 4 ampicillin-resistant H. parainfluenzae isolates for plasmid deoxyribonucleic acid. Sixteen strains carried plasmids. Both "plasmid-free" and plasmid-carrying isolates transferred the antibiotic resistance by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.1.476-479.1980

    authors: Roberts MC,Smith AL

    更新日期:1980-10-01 00:00:00

  • Regulatory circuit for responses of nitrogen catabolic gene expression to the GLN3 and DAL80 proteins and nitrogen catabolite repression in Saccharomyces cerevisiae.

    abstract::We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. The expression of all these genes, with the exception of UGA1 and PUT2, also required a functional GLN3 protein. In addition, GLN3 protein was required for expression of the DAL1, DA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.1.64-73.1993

    authors: Daugherty JR,Rai R,el Berry HM,Cooper TG

    更新日期:1993-01-01 00:00:00

  • Effects of different carbon fluxes on G1 phase duration, cyclin expression, and reserve carbohydrate metabolism in Saccharomyces cerevisiae.

    abstract::By controlled addition of galactose to synchronized galactose-limited Saccharomyces cerevisiae cultures, the growth rate could be regulated while external conditions were kept constant. By using this method, the G1 phase duration was modulated and expression of cell cycle-regulated genes was investigated. The expressi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6560-6565.1997

    authors: Silljé HH,ter Schure EG,Rommens AJ,Huls PG,Woldringh CL,Verkleij AJ,Boonstra J,Verrips CT

    更新日期:1997-11-01 00:00:00

  • Differential substrate specificity and kinetic behavior of Escherichia coli YfdW and Oxalobacter formigenes formyl coenzyme A transferase.

    abstract::The yfdXWUVE operon appears to encode proteins that enhance the ability of Escherichia coli MG1655 to survive under acidic conditions. Although the molecular mechanisms underlying this phenotypic behavior remain to be elucidated, findings from structural genomic studies have shown that the structure of YfdW, the prote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01823-07

    authors: Toyota CG,Berthold CL,Gruez A,Jónsson S,Lindqvist Y,Cambillau C,Richards NG

    更新日期:2008-04-01 00:00:00

  • The Conserved DNA Binding Protein WhiA Influences Chromosome Segregation in Bacillus subtilis.

    abstract::The DNA binding protein WhiA is conserved in Gram-positive bacteria and is present in the genetically simple cell wall-lacking mycoplasmas. The protein shows homology to eukaryotic homing endonucleases but lacks nuclease activity. WhiA was first characterized in streptomycetes, where it regulates the expression of key...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00633-17

    authors: Bohorquez LC,Surdova K,Jonker MJ,Hamoen LW

    更新日期:2018-03-26 00:00:00

  • Iron transport in Mycobacterium smegmatis: Uptake of iron from ferric citrate.

    abstract::In mycobacterial growth medium 40 to 400 microM citrate was required to solubilize 2 microM 55Fe. This solubilized 55Fe was taken up into both iron-deficient and iron sufficient washed cell suspensions of Mycobacterium smegmatis and Mycobacterium bovis BCG. Although the 55Fe was taken up into the cell, the citrate was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.1.131-135.1982

    authors: Messenger AJ,Ratledge C

    更新日期:1982-01-01 00:00:00

  • Dentilisin activity affects the organization of the outer sheath of Treponema denticola.

    abstract::Prolyl-phenylalanine-specific serine protease (dentilisin) is a major extracellular protease produced by Treponema denticola. The gene, prtP, coding for the protease was recently cloned and sequenced (K. Ishihara, T. Miura, H. K. Kuramitsu, and K. Okuda, Infect. Immun. 64:5178-5186, 1996). In order to determine the ro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.15.3837-3844.1998

    authors: Ishihara K,Kuramitsu HK,Miura T,Okuda K

    更新日期:1998-08-01 00:00:00

  • A third beta-galactosidase in a strain of Klebsiella that possesses two lac genes.

    abstract::Klebsiella strain RE1544 contains two lac operons, one on the chromosome and one on a lac plasmid. A mutant of RE1544, in which the lacZ genes of both operons produce no active enzyme, was found to synthesize a beta-galactosidase that hydrolyzes ortho-nitrophenyl-beta-D-galactopyranoside but not lactose. Synthesis of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.1.219-223.1977

    authors: Hall BG,Reeve EC

    更新日期:1977-10-01 00:00:00

  • Unbalanced growth death due to depletion of Mn2+ in Brevibacterium ammoniagenes.

    abstract::In the microbial conversion of added hypoxanthine to 5'-inosinic acid, Mn(2+) concentration in the growth medium is known to have a profound effect both on the yield of 5'-inosinic acid and the morphology of cells of Brevibacterium ammoniagenes. To elucidate the mechanism in which Mn(2+) was concerned with cell morpho...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.5.1760-1767.1968

    authors: Oka T,Udagawa K,Kinoshita S

    更新日期:1968-11-01 00:00:00

  • Site-directed mutagenesis of the mecA gene from a methicillin-resistant strain of Staphylococcus aureus.

    abstract::The mecA-27r gene from Staphylococcus aureus 27r encodes penicillin-binding protein 2a (PBP2a-27r), which causes this strain to be methicillin resistant. Removal or replacement of the N-terminal transmembrane domain had no effect on binding of penicillin, but removal of portions of the putative transglycosylase domain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.2.443-449.1994

    authors: Wu CY,Alborn WE Jr,Flokowitsch JE,Hoskins J,Unal S,Blaszczak LC,Preston DA,Skatrud PL

    更新日期:1994-01-01 00:00:00

  • Bacterial-type ferredoxin genes in the nitrogen fixation regions of the cyanobacterium Anabaena sp. strain PCC 7120 and Rhizobium meliloti.

    abstract::The nucleotide sequence of a region located downstream of the nifB gene, both in the cyanobacterium Anabaena sp. strain PCC 7120 and in Rhizobium meliloti, has been determined. This region contains a gene (fdxN) whose predicted polypeptide product strongly resembles typical bacterial ferredoxins. Cyanobacteria have no...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.9.4406-4410.1988

    authors: Mulligan ME,Buikema WJ,Haselkorn R

    更新日期:1988-09-01 00:00:00

  • The bZip transcription factor Cap1p is involved in multidrug resistance and oxidative stress response in Candida albicans.

    abstract::Candida albicans is an opportunistic pathogenic yeast which frequently develops resistance to the antifungal agent fluconazole (FCZ) in patients undergoing long-term therapy. FCZ-resistant strains often display a reduced intracellular FCZ accumulation which correlates with the overexpression of the ATP-binding cassett...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.3.700-708.1999

    authors: Alarco AM,Raymond M

    更新日期:1999-02-01 00:00:00

  • Complete genome sequence of Lactobacillus plantarum JDM1.

    abstract::Lactobacillus plantarum is a lactic acid bacterium (LAB) species commonly used as a probiotic. We have sequenced the genome of Lactobacillus plantarum JDM1, which is a Chinese commercial LAB with several probiotic functions, using a GS 20 system. We recommend that each commercial probiotic strain should undergo comple...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00587-09

    authors: Zhang ZY,Liu C,Zhu YZ,Zhong Y,Zhu YQ,Zheng HJ,Zhao GP,Wang SY,Guo XK

    更新日期:2009-08-01 00:00:00

  • Isozymes of beta-glucosidase in Dictyostelium discoideum.

    abstract::The activity of beta-glucosidase (EC 3.2.1.21) in extracts of Dictyostelium discoideum was investigated. The specific activity increased early in development, declined during pseudoplasmodium formation, and increased again during sorocarp formation. The beta-glucosidase which was present in growing amoebae and during ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.3.1208-1217.1969

    authors: Coston MB,Loomis WF Jr

    更新日期:1969-12-01 00:00:00

  • Occurrence of D-rhamnan as the common antigen reactive against monoclonal antibody E87 in Pseudomonas aeruginosa IFO 3080 and other strains.

    abstract::S. Sawada and co-workers reported that a monoclonal antibody (MAb), E87, interacted with about 80% of Pseudomonas aeruginosa isolates, and they separated a rhamnose-rich polysaccharide as the probable antigen for MAb E87 from P. aeruginosa IFO 3080 (S. Sawada, T. Kawamura, Y. Masuho, and K. Tomibe, J. Infec. Dis. 152:...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.6162-6164.1990

    authors: Yokota S,Kaya S,Araki Y,Ito E,Kawamura T,Sawada S

    更新日期:1990-10-01 00:00:00

  • Yield coefficients of Thiobacillus neapolitanus in continuous culture.

    abstract::Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.3.874-878.1967

    authors: Hempfling WP,Vishniac W

    更新日期:1967-03-01 00:00:00

  • Role of the C terminus of FtsK in Escherichia coli chromosome segregation.

    abstract::FtsK is essential for Escherichia coli cell division. We report that cells lacking the C terminus of FtsK are defective in chromosome segregation as well as septation, often exhibiting asymmetrically positioned nucleoids and large anucleate regions. Combining the corresponding truncated ftsK gene with a mukB null muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Yu XC,Weihe EK,Margolin W

    更新日期:1998-12-01 00:00:00

  • Carbon catabolite repression of maltase synthesis in Saccharomyces carlsbergensis.

    abstract::Carbon catabolite repression of maltase gene expression is brought about by the addition of glucose, resulting in a drastic inhibition of the induction of maltase. When added to induced cells, glucose leads to the inhibition of maltase synthesis within 30 min, which can be accounted for by the disappearance of hybridi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.1.301-307.1983

    authors: Federoff HJ,Eccleshall TR,Marmur J

    更新日期:1983-10-01 00:00:00

  • Transport of vitamin B12 in Escherichia coli: common receptor system for vitamin B12 and bacteriophage BF23 on the outer membrane of the cell envelope.

    abstract::We showed previously that the outer membrane of the Escherichia coli cell envelope normally contains about 200 to 250 B12 receptors, and that these receptors function both in B12 transport and as receptors for the E colicins. This paper shows that this receptor system is also shared with bacteriophage BF23. A strong p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.3.1032-1039.1976

    authors: Bradbeer C,Woodrow ML,Khalifah LI

    更新日期:1976-03-01 00:00:00

  • The Proline Variant of the W[F/L/M][T/S]R Cyclic Di-GMP Binding Motif Suppresses Dependence on Signal Association for Regulator Function.

    abstract::Vibrio vulnificus is an estuarine bacterium and potent opportunistic human pathogen. It enters the food chain by asymptomatically colonizing a variety of marine organisms, most notably oysters. Expression of the brp-encoded extracellular polysaccharide, which enhances cell-surface adherence, is regulated by cyclic di-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00344-17

    authors: Chodur DM,Guo L,Pu M,Bruger E,Fernandez N,Waters C,Rowe-Magnus DA

    更新日期:2017-09-05 00:00:00

  • Escherichia coli peptidase A, B, or N can process translation inhibitor microcin C.

    abstract::The heptapeptide-nucleotide microcin C (McC) targets aspartyl-tRNA synthetase. Upon its entry into a susceptible cell, McC is processed to release a nonhydrolyzable aspartyl-adenylate that inhibits aspartyl-tRNA synthetase, leading to the cessation of translation and cell growth. Here, we surveyed Escherichia coli cel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01956-07

    authors: Kazakov T,Vondenhoff GH,Datsenko KA,Novikova M,Metlitskaya A,Wanner BL,Severinov K

    更新日期:2008-04-01 00:00:00