Transport of vitamin B12 in Escherichia coli: common receptor system for vitamin B12 and bacteriophage BF23 on the outer membrane of the cell envelope.

abstract：:Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially char...

journal_title：Journal of bacteriology

authors： Currier TC,Haury JF,Wolk CP

更新日期：1977-03-01 00:00:00

abstract：:The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gen...

journal_title：Journal of bacteriology

authors： Metcalf WW,Zhang JK,Shi X,Wolfe RS

更新日期：1996-10-01 00:00:00

abstract：:During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection a...

journal_title：Journal of bacteriology

authors： Daborn PJ,Waterfield N,Blight MA,Ffrench-Constant RH

更新日期：2001-10-01 00:00:00

abstract：:The heat shock response of the groESL operon of Agrobacterium tumefaciens was studied at the RNA level. The operon was found to be activated under heat shock conditions and transcribed as a polycistronic mRNA that contains the groES and groEL genes. After activation, the polycistronic mRNA appeared to be cleaved betwe...

journal_title：Journal of bacteriology

authors： Segal G,Ron EZ

更新日期：1995-02-01 00:00:00

abstract：:Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...

journal_title：Journal of bacteriology

authors： Brunskill EW,Bayles KW

更新日期：1996-02-01 00:00:00

abstract：:Specific labeling of a single row of cellulose-synthesizing complexes (terminal complexes, TC subunits, TCs, or TC arrays) in Acetobacter xylinum by antibodies raised against a 93-kDa protein (the cyclic dignanylic acid-binding protein) has been demonstrated by using the sodium dodecyl sulfate (SDS)-freeze-fracture la...

journal_title：Journal of bacteriology

authors： Kimura S,Chen HP,Saxena IM,Brown RM Jr,Itoh T

更新日期：2001-10-01 00:00:00

abstract：:A primary determinant of pathogenicity in Erwinia stewartii is the production of extracellular polysaccharide (EPS). A single mutation can abolish both EPS synthesis and pathogenicity; both properties are restored by a single cosmid clone. Subcloning and insertion analysis have defined a single positive regulatory fun...

journal_title：Journal of bacteriology

authors： Torres-Cabassa A,Gottesman S,Frederick RD,Dolph PJ,Coplin DL

更新日期：1987-10-01 00:00:00

abstract：:A catabolite-sensitive promoter was found to be involved in transcription of the heat shock regulatory gene rpoH encoding the sigma 32 protein. Expression of lacZ from the operon fusion, rpoHp-lacZ, was partially inhibited by glucose added to the broth medium. Dissection of the rpoH promoter region allowed us to local...

journal_title：Journal of bacteriology

authors： Nagai H,Yano R,Erickson JW,Yura T

更新日期：1990-05-01 00:00:00

abstract：:Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residu...

journal_title：Journal of bacteriology

authors： Wang J,Hofnung M,Charbit A

更新日期：2000-01-01 00:00:00

abstract：:Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with...

journal_title：Journal of bacteriology

authors： Vales LD,Chase JW,Murphy JB

更新日期：1979-07-01 00:00:00

abstract：:A new mutation in Escherichia coli, designated gcvA1, that results in noninducible expression of both gcv and a gcvT-lacZ gene fusion was isolated. A plasmid carrying the wild-type gcvA gene complemented the mutation and restored glycine-inducible gcv and gcvT-lacZ gene expression. These results suggest that gcvA enco...

journal_title：Journal of bacteriology

authors： Wilson RL,Steiert PS,Stauffer GV

更新日期：1993-02-01 00:00:00

abstract：:Sequences of the major outer membrane protein (MOMP) gene (ompA) and the outer membrane complex B protein gene (omcB) from Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci were analyzed for evidence of intragenic recombination and for linkage equilibrium. The Sawyer runs test, compatibility matrices...

journal_title：Journal of bacteriology

authors： Millman KL,Tavaré S,Dean D

更新日期：2001-10-01 00:00:00

abstract：:We have reinvestigated the genetic organization and the transcription regulation of the dsd operon of Escherichia coli. By combining genetic and biochemical studies, it is demonstrated that the regulatory region of the operon and the gene encoding the specific regulator of D-serine tolerance (dsdC) had been misplaced ...

journal_title：Journal of bacteriology

authors： Nørregaard-Madsen M,McFall E,Valentin-Hansen P

更新日期：1995-11-01 00:00:00

abstract：:Sinorhizobium meliloti is a soil-dwelling alphaproteobacterium that engages in a nitrogen-fixing root nodule symbiosis with leguminous plants. Cell surface polysaccharides are important both for adapting to stresses in the soil and for the development of an effective symbiotic interaction. Among the polysaccharides ch...

journal_title：Journal of bacteriology

authors： Barnett MJ,Long SR

更新日期：2018-01-10 00:00:00

abstract：:In contrast to Escherichia coli and Salmonella typhimurium, Bacillus subtilis could convert ethionine to S-adenosylethionine (SAE), as can Saccharomyces cerevisiae. This conversion was essential for growth inhibition by ethionine because metE mutants which were deficient in S-adenosylmethionine synthetase activity, we...

journal_title：Journal of bacteriology

authors： Allen ER,Orrego C,Wabiko H,Freese E

更新日期：1986-04-01 00:00:00

abstract：:The bacterium Myxococcus xanthus alternates between two colony types distinguished by colony morphology and pigmentation. Because the two phases are interconvertible, this phenomenon has been termed phase variation. In one phase, the colonies are bright yellow, rough, and swarming. In the alternate phase, the colonies...

journal_title：Journal of bacteriology

authors： Laue BE,Gill RE

更新日期：1994-09-01 00:00:00

abstract：:Growth of Streptomyces viridochromogenes on a solid glycerol-NH4NO3 salts medium was accompanied by the formation of aerial mycelia and spores. Adding 0.5% or more casein hydrolysate to the medium stimulated growth while completely repressing the formation of aerial mycelia and spores. This repression was temporary, a...

journal_title：Journal of bacteriology

authors： Coleman RH,Ensign JC

更新日期：1982-03-01 00:00:00

abstract：:The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The r...

journal_title：Journal of bacteriology

authors： Gustafsson P,Nordström K

更新日期：1975-08-01 00:00:00

abstract：:OxyR is a DNA binding protein that differentially regulates a cell's response to hydrogen peroxide-mediated oxidative stress. We previously reported that the reduced form of OxyR is sufficient for repression of transcription of agn43 from unmethylated template DNA, which is essential for deoxyadenosine methylase (Dam)...

journal_title：Journal of bacteriology

authors： Wallecha A,Correnti J,Munster V,van der Woude M

更新日期：2003-04-01 00:00:00

abstract：:The DNA sequence of a 3.23-kilobase fragment of the Escherichia coli chromosome encoding biosynthetic arginine decarboxylase (ADC) was determined. This sequence contained the speA open reading frame (ORF) as well as partial speB and metK ORFs. The ADC ORF is 1,974 nucleotides long; the deduced polypeptide contains 658...

journal_title：Journal of bacteriology

authors： Moore RC,Boyle SM

更新日期：1990-08-01 00:00:00

abstract：:Streptococcus oralis, a commensal species of the human oral cavity, belongs to the Mitis group of streptococci, which includes one of the major human pathogens as well, S. pneumoniae. We report here the first complete genome sequence of this species. S. oralis Uo5, a high-level penicillin- and multiple-antibiotic-resi...

journal_title：Journal of bacteriology

authors： Reichmann P,Nuhn M,Denapaite D,Brückner R,Henrich B,Maurer P,Rieger M,Klages S,Reinhard R,Hakenbeck R

更新日期：2011-06-01 00:00:00

abstract：:In Rhizobium meliloti, an aspartate aminotransferase (AspAT) encoded within a 7.3-kb HindIII fragment was previously shown to be required for symbiotic nitrogen fixation and aspartate catabolism (V. K. Rastogi and R.J. Watson, J. Bacteriol. 173:2879-2887, 1991). A gene coding for an aromatic aminotransferase located w...

journal_title：Journal of bacteriology

authors： Watson RJ,Rastogi VK

更新日期：1993-04-01 00:00:00

abstract：:Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...

journal_title：Journal of bacteriology

authors： Madigan MT,Brock TD

更新日期：1975-05-01 00:00:00

abstract：:Periplasmic nitrate reductase (NapABC enzyme) has been characterized from a variety of proteobacteria, especially Paracoccus pantotrophus. Whole-genome sequencing of Escherichia coli revealed the structural genes napFDAGHBC, which encode NapABC enzyme and associated electron transfer components. E. coli also expresses...

journal_title：Journal of bacteriology

authors： Stewart V,Lu Y,Darwin AJ

更新日期：2002-03-01 00:00:00

abstract：:Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the "downhill" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhyd...

journal_title：Journal of bacteriology

authors： Cecchini G,Koch AL

更新日期：1975-07-01 00:00:00

abstract：:The polymerase chain reaction (PCR) has been used to amplify DNA fragments by using eucaryotic genomic DNA as a template. We show that bacterial genomic DNA can be used as a template for PCR amplification. We demonstrate that DNA fragments at least as large as 4,400 base pairs can be amplified with fidelity and that t...

journal_title：Journal of bacteriology

authors： Shyamala V,Ames GF

更新日期：1989-03-01 00:00:00

abstract：:Escherichia coli K-12 deficient in galactose-1-phosphate uridyl transferase is capable of converting significant amounts of d-[1-(14)C]galactose to (14)CO(2), whereas strains deficient in other enzymes of the Leloir pathway cannot do so. ...

journal_title：Journal of bacteriology

authors： LaPolla RJ,Geier MR,Friedman TB,Merril CR

更新日期：1975-10-01 00:00:00

abstract：:Streptococcus faecalis transposon Tn917 was cloned in Escherichia coli on plasmid vector pBR325. The erythromycin resistance determinant of Tn917 was not expressed in the E. coli background. The nucleotide sequence of Tn917 was determined and found to be 5,257 base pairs in length. Six open reading frames (ORFs) were ...

journal_title：Journal of bacteriology

authors： Shaw JH,Clewell DB

更新日期：1985-11-01 00:00:00

abstract：:The 6th American Society for Microbiology Conference on Cell-Cell Communication in Bacteria convened from 16 to 19 October 2017 in Athens, GA. In this minireview, we highlight some of the research presented at that meeting that addresses central questions emerging in the field, including the following questions. How a...

journal_title：Journal of bacteriology

authors： Brown SP,Blackwell HE,Hammer BK

更新日期：2018-06-25 00:00:00

abstract：:We report on novel mutations in the malK gene of Salmonella enterica serovar Typhimurium, encoding the ATPase subunit of the maltose transporter (MalFGK(2)). Biochemical analysis suggests that (i) L86 might be involved in a signaling step during substrate translocation and (ii) E306 may be critical for the structural ...

journal_title：Journal of bacteriology

authors： Hunke S,Landmesser H,Schneider E

更新日期：2000-03-01 00:00:00