Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium.

Abstract:

:Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially characterized by replica plating to media supplemented with single nutrients or specific groups of nutrients. Of the 83 auxotrophs isolated, 65 required an inorganic source of nitrogen for growth. In addition, auxotrophs were isolated that required methionine (six), uracil (two), adenine (one), biotin (two), and nicotinic acid (two). (The number of isolates of each type is indicated in parentheses.) The nutrient requirements of five auxotrophs appeared complex and were not determined. A large proportion of the mutants requiring inorgainic fixed nitrogen was altered in the differentiation of heterocysts. The following morphological aberrancies were observed: abnormally high and abnormally low frequencies of heterocysts; thick, uneven heterocyst envelopes; incompletely developed pore regions; very distinct pore regions; and protoplasts separated from the envelope of the heterocyst. Spontaneously occurring, N2-fixing, prototrophic revertants of mutants with aberrant heterocysts have been isolated at a frequency of 2 X 10(-8) to 4 X 10(-8) of the cells plated. That most such revertants produced morphologically normal heterocysts is consisten with the idea that heterocysts play an essential role in aerobic N2 fixation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Currier TC,Haury JF,Wolk CP

doi

10.1128/JB.129.3.1556-1562.1977

subject

Has Abstract

pub_date

1977-03-01 00:00:00

pages

1556-62

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

129

pub_type

杂志文章
  • Purification and properties of a hydrogenase from Desulfovibrio vulgaris.

    abstract::The soluble hydrogenase of Desulfovibrio vulgaris was purified and some of its properties are described. The molecular weight was determined for the enzyme by sedimentation equilibrium (45,400) and amino acid analysis (44,800). The hydrogenase appears to be a loosely coiled molecule or to have a high axial ratio, whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.1.249-258.1971

    authors: Haschke RH,Campbell LL

    更新日期:1971-01-01 00:00:00

  • Isolation of a mutant of Escherichia coli defective in cytosine-specific deoxyribonucleic acid methylase activity and in partial protection of bacteriophage lambda against restriction by cells containing the N-3 drug-resistance factor.

    abstract::A mutant (designated mec(-)) of Escherichia coli F(+) 100 endo I(-)su(+) r(K) (-)m(K) (+) has been isolated which is defective in cytosine-specific deoxyribonucleic acid (DNA) methylase activity. The DNA of this mutant, as well as the DNA of phages lambda and fd propagated in it, is virtually devoid of 5-methyl-cytosi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.3.1103-1107.1973

    authors: Hattman S,Schlagman S,Cousens L

    更新日期:1973-09-01 00:00:00

  • Genome-wide expression analysis indicates that FNR of Escherichia coli K-12 regulates a large number of genes of unknown function.

    abstract::The major regulator controlling the physiological switch between aerobic and anaerobic growth conditions in Escherichia coli is the DNA binding protein FNR. To identify genes controlled by FNR, we used Affymetrix Antisense GeneChips to compare global gene expression profiles from isogenic MG1655 wild-type and Deltafnr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.3.1135-1160.2005

    authors: Kang Y,Weber KD,Qiu Y,Kiley PJ,Blattner FR

    更新日期:2005-02-01 00:00:00

  • Detection and characterization of conjugative degradative plasmids in xenobiotic-degrading Sphingomonas strains.

    abstract::A systematic survey for the presence of plasmids in 17 different xenobiotic-degrading Sphingomonas strains was performed. In almost all analyzed strains, two to five plasmids with sizes of about 50 to 500 kb were detected by using pulsed-field gel electrophoresis. A comparison of plasmid preparations untreated or trea...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.12.3862-3872.2004

    authors: Basta T,Keck A,Klein J,Stolz A

    更新日期:2004-06-01 00:00:00

  • Acetohydroxy acid synthase I is required for isoleucine and valine biosynthesis by Salmonella typhimurium LT2 during growth on acetate or long-chain fatty acids.

    abstract::Salmonella typhimurium LT2 normally expresses two acetohydroxy acid synthases (AHAS I and AHAS II). The function of AHAS I in this organism was unclear, since AHAS I-deficient (ilvBN) mutants of LT2 grew well on glucose or succinate minimal media, whereas AHAS II-deficient (ilvGM) mutants requried isoleucine for norma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.917-919.1987

    authors: Dailey FE,Cronan JE Jr,Maloy SR

    更新日期:1987-02-01 00:00:00

  • Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli.

    abstract::The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli. CsrA activity is antagonized by the untranslated RNA CsrB, to which it binds and forms a globula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.18.5130-5140.2002

    authors: Suzuki K,Wang X,Weilbacher T,Pernestig AK,Melefors O,Georgellis D,Babitzke P,Romeo T

    更新日期:2002-09-01 00:00:00

  • Molecular cloning and transcriptional analysis of a guanosine kinase gene of Brevibacterium acetylicum ATCC 953.

    abstract::The Brevibacterium acetylicum gsk gene, which encodes guanosine kinase (ATP:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the N-terminal amino acid sequence of the purified protein. The cloned chromosomal fragment containing the gsk gene was sequenc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.6959-6964.1997

    authors: Usuda Y,Kawasaki H,Shimaoka M,Utagawa T

    更新日期:1997-11-01 00:00:00

  • Domains within RbpA Serve Specific Functional Roles That Regulate the Expression of Distinct Mycobacterial Gene Subsets.

    abstract::The RNA polymerase (RNAP) binding protein A (RbpA) contributes to the formation of stable RNAP-promoter open complexes (RPo) and is essential for viability in mycobacteria. Four domains have been identified in the RbpA protein, i.e., an N-terminal tail (NTT) that interacts with RNAP β' and σ subunits, a core domain (C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00690-17

    authors: Prusa J,Jensen D,Santiago-Collazo G,Pope SS,Garner AL,Miller JJ,Ruiz Manzano A,Galburt EA,Stallings CL

    更新日期:2018-06-11 00:00:00

  • Investigation of two evolutionarily unrelated halocarboxylic acid dehalogenase gene families.

    abstract::Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.8.2535-2547.1999

    authors: Hill KE,Marchesi JR,Weightman AJ

    更新日期:1999-04-01 00:00:00

  • Escherichia coli mutants with an altered sensitivity to cecropin D.

    abstract::Cecropins are a family of small, basic antibacterial polypeptides which can be isolated from pupae of immunized Lepidoptera. They are active against both gram-negative and gram-positive bacteria. We studied a mutant of Escherichia coli, strain SB1004, which is more sensitive to cecropin D than is the parental strain. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.1.170-176.1983

    authors: Sidén I,Boman HG

    更新日期:1983-04-01 00:00:00

  • Assemblies of DivIVA mark sites for hyphal branching and can establish new zones of cell wall growth in Streptomyces coelicolor.

    abstract::Time-lapse imaging of Streptomyces hyphae revealed foci of the essential protein DivIVA at sites where lateral branches will emerge. Overexpression experiments showed that DivIVA foci can trigger establishment of new zones of cell wall assembly, suggesting a key role of DivIVA in directing peptidoglycan synthesis and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00839-08

    authors: Hempel AM,Wang SB,Letek M,Gil JA,Flärdh K

    更新日期:2008-11-01 00:00:00

  • In Silico Discovery and In Vitro Validation of Catechol-Containing Sulfonohydrazide Compounds as Potent Inhibitors of the Diguanylate Cyclase PleD.

    abstract:UNLABELLED:Biofilm formation is responsible for increased antibiotic tolerance in pathogenic bacteria. Cyclic di-GMP (c-di-GMP) is a widely used second-messenger signal that plays a key role in bacterial biofilm formation. c-di-GMP is synthesized by diguanylate cyclases (DGCs), a conserved class of enzymes absent in ma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00742-15

    authors: Fernicola S,Paiardini A,Giardina G,Rampioni G,Leoni L,Cutruzzolà F,Rinaldo S

    更新日期:2015-09-28 00:00:00

  • Functions of the duplicated hik31 operons in central metabolism and responses to light, dark, and carbon sources in Synechocystis sp. strain PCC 6803.

    abstract::There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to exami...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06207-11

    authors: Nagarajan S,Sherman DM,Shaw I,Sherman LA

    更新日期:2012-01-01 00:00:00

  • Differential Reduction of Tellurite by Growing Colonies of Normal Yeast and Respiration-Deficient Mutants.

    abstract::Nagai, Susumu (National Women's University, Nara, Japan). Differential reduction of tellurite by growing colonies of normal yeast and respiration-deficient mutants. J. Bacteriol. 90:220-222. 1965.-A differential reduction of sodium tellurite was observed between normal and respiration-deficient mutant colonies of seve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.1.220-222.1965

    authors: Nagai S

    更新日期:1965-07-01 00:00:00

  • Collaborative regulation of Escherichia coli glutamate-dependent acid resistance by two AraC-like regulators, GadX and GadW (YhiW).

    abstract::An important feature of Escherichia coli pathogenesis is an ability to withstand extremely acidic environments of pH 2 or lower. This acid resistance property contributes to the low infectious dose of pathogenic E. coli species. One very efficient E. coli acid resistance system encompasses two isoforms of glutamate de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.24.7001-7012.2002

    authors: Ma Z,Richard H,Tucker DL,Conway T,Foster JW

    更新日期:2002-12-01 00:00:00

  • Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain.

    abstract::The Escherichia coli dnaN159 allele encodes a mutant form of the beta-sliding clamp (beta159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. In addition, strains bearing the dnaN159 allele require functional Pol I for viability. We have utilized a combination of genetic and bioche...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00476-07

    authors: Maul RW,Sanders LH,Lim JB,Benitez R,Sutton MD

    更新日期:2007-07-01 00:00:00

  • Targeting the Achilles' heel of bacteria: different mechanisms to break down the peptidoglycan cell wall during bacterial warfare.

    abstract::Bacteria commonly live in dense polymicrobial communities and compete for scarce resources. Consequently, they employ a diverse array of mechanisms to harm, inhibit and kill their competitors. The cell wall is essential for bacterial survival by providing mechanical strength to resist osmotic stress. Because peptidogl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00478-20

    authors: Sibinelli-Sousa S,Hespanhol JT,Bayer-Santos E

    更新日期:2020-11-02 00:00:00

  • Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis.

    abstract::Fukuyama T. T. (University of Pennsylvania, Philadelphia) and D. J. O'Kane. Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis. J. Bacteriol. 84:793-796. 1962.-The pathway by which galactose-1-C(14) is fermented in Streptococcus faecalis was investigated using dried-cell preparations....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.4.793-796.1962

    authors: FUKUYAMA TT,O'KANE DJ

    更新日期:1962-10-01 00:00:00

  • Localization of cytochromes to the outer membrane of anaerobically grown Shewanella putrefaciens MR-1.

    abstract::In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.11.3429-3438.1992

    authors: Myers CR,Myers JM

    更新日期:1992-06-01 00:00:00

  • Multicellular stalk-like structures in Saccharomyces cerevisiae.

    abstract::Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproduc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.15.3992-3996.1998

    authors: Engelberg D,Mimran A,Martinetto H,Otto J,Simchen G,Karin M,Fink GR

    更新日期:1998-08-01 00:00:00

  • Purification and characterization of an exo-beta-1,4-glucanase from Ruminococcus flavefaciens FD-1.

    abstract::An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter paper was deg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4581-4588.1987

    authors: Gardner RM,Doerner KC,White BA

    更新日期:1987-10-01 00:00:00

  • trp RNA-binding attenuation protein (TRAP)-trp leader RNA interactions mediate translational as well as transcriptional regulation of the Bacillus subtilis trp operon.

    abstract::Expression of the Bacillus subtilis trpEDCFBA operon has been shown to be regulated by transcription attenuation in response to the availability of L-tryptophan. Regulation is mediated by the tryptophan-activated trp RNA-binding attenuation protein, TRAP, the product of mtrB. Formation of mutually exclusive RNA anti-t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.22.6362-6370.1995

    authors: Merino E,Babitzke P,Yanofsky C

    更新日期:1995-11-01 00:00:00

  • Regulation of DNA superhelicity by rpoB mutations that suppress defective Rho-mediated transcription termination in Escherichia coli.

    abstract::The highly defective rho-15 mutant of Escherichia coli produces plasmid DNA that is 22% less negatively supercoiled than DNA from an isogenic wild-type strain (J. S. Fassler, G. F. Arnold, and I. Tessman, Mol. Gen. Genet. 204:424-429, 1986). We extended our measurements of plasmid superhelicity to additional rho mutan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.9.4266-4271.1988

    authors: Arnold GF,Tessman I

    更新日期:1988-09-01 00:00:00

  • Immunocytochemical localization of a calmodulinlike protein in Bacillus subtilis cells.

    abstract::To determine possible functions of the calmodulinlike protein of Bacillus subtilis, the time course of its expression during sporulation and its cellular localization were studied. The protein was expressed in a constitutive manner from the end of logarithmic growth through 8 h of sporulation as determined by antibody...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.15.4605-4610.1999

    authors: Dominguez DC,Adams H,Hageman JH

    更新日期:1999-08-01 00:00:00

  • Separation and characterization of six (1 leads to 3)-beta-glucanases from Saccharomyces cerevisiae.

    abstract::Using a system of chromatography through columns of DEAE-Bio-Gel, HTP-Bio-Gel, and CM-Bio-Gel, we isolated and characterized six different (1 leads to 3)-beta-glucanases from cell wall autolysates and cell extracts of Saccharomyces cerevisiae haploid strain 2180B. These enzymes were designated glucanases I, II, IIIA, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.3.1204-1213.1983

    authors: Hien NH,Fleet GH

    更新日期:1983-12-01 00:00:00

  • A polypurine sequence that acts as a 5' mRNA stabilizer in Bacillus subtilis.

    abstract::A segment of early RNA from Bacillus subtilis bacteriophage SP82 was shown to function as a 5' stabilizer in B. subtilis. Several heterologous RNA sequences were stabilized by the presence of the SP82 sequence at the 5' end, and expression of downstream coding sequences was increased severalfold. The SP82 RNA segment ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.12.3465-3471.1995

    authors: Hue KK,Cohen SD,Bechhofer DH

    更新日期:1995-06-01 00:00:00

  • Expression of glnA in the cyanobacterium Synechococcus sp. strain PCC 7942 is initiated from a single nif-like promoter under various nitrogen conditions.

    abstract::The glnA mRNA, encoding glutamine synthetase, is differentially accumulated in the cyanobacterium Synechococcus sp. strain PCC 7942 in media containing different nitrogen sources. With the different nitrogen compounds, transcription of glnA initiated at a single site located -146 nucleotides upstream of the translatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.23.7727-7731.1993

    authors: Cohen-Kupiec R,Gurevitz M,Zilberstein A

    更新日期:1993-12-01 00:00:00

  • Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D.

    abstract::The gene (entD) encoding staphylococcal enterotoxin D (SED) has been located on a 27.6-kilobase penicillinase plasmid designated pIB485. This plasmid was present in all SED-producing strains tested. The entD gene was cloned on a 2.0-kilobase DNA fragment and was expressed in Escherichia coli. Sequence analysis of this...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4799-4806.1989

    authors: Bayles KW,Iandolo JJ

    更新日期:1989-09-01 00:00:00

  • Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium.

    abstract::A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.3.854-859.1984

    authors: Von Tersch MA,Carlton BC

    更新日期:1984-12-01 00:00:00

  • Site-specific recombination system encoded by toluene catabolic transposon Tn4651.

    abstract::The 56-kb class II toluene catabolic transposon Tn4651 from Pseudomonas putida plasmid pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution (res) site, the two gene products TnpS and TnpT and (ii) the 2.4-kb tnpT-res-tnpS region is 48 kb apart from the tnpA gene (M. Tsuda,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.17.4757-4766.2002

    authors: Genka H,Nagata Y,Tsuda M

    更新日期:2002-09-01 00:00:00