Abstract:
:Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the "downhill" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhydrazone results in stimulation of ONPG transport. The same agents lower transport of non-energy-depleted cells towards the same levels that result from stimulation of the energy depleted cells. Of course, these agents prevent "uphill" accumulation against a concentration gradient under all conditions. Since uncouplers allow normal and energy-depleted cells to assume the same facilitated transport capability, these results lend support to the chemiosmotic hypothesis of Mitchell that comigration of charge is necessary for the transport of neutral galactosides. Our results imply that a potential favorable to transport is maintained by metabolism in non-energy-depleted cells, whereas an unfavorable potential is developed in the initial instant of time when energy-depleted cells are given ONPG.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Cecchini G,Koch ALdoi
10.1128/JB.123.1.187-195.1975keywords:
subject
Has Abstractpub_date
1975-07-01 00:00:00pages
187-95issue
1eissn
0021-9193issn
1098-5530journal_volume
123pub_type
杂志文章abstract::In Salmonella enterica serovar Typhimurium a mutation in the purF gene encoding the first enzyme in the purine pathway blocks, besides the synthesis of purine, the synthesis of thiamine when glucose is used as the carbon source. On carbon sources other than glucose, a purF mutant does not require thiamine, since the a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.3.750-759.2003
更新日期:2003-02-01 00:00:00
abstract::Micromonospora strains have been isolated from diverse niches, including soil, water, and marine sediments and root nodules of diverse symbiotic plants. In this work, we report the genome sequence of Micromonospora lupini Lupac 08 isolated from root nodules of the wild legume Lupinus angustifolious. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00628-12
更新日期:2012-08-01 00:00:00
abstract::Chlamydia trachomatis lacks the canonical genes required for the biosynthesis of p-aminobenzoate (pABA), a component of essential folate cofactors. Previous studies revealed a single gene from C. trachomatis, the CT610 gene, that rescues Escherichia coli ΔpabA, ΔpabB, and ΔpabC mutants, which are otherwise auxotrophic...
journal_title:Journal of bacteriology
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更新日期:2020-09-23 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00002-09
更新日期:2009-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5669-5672.1988
更新日期:1988-12-01 00:00:00
abstract::The PDC1 gene coding for a pyruvate decarboxylase (PDC; EC 4.1.1.1) was deleted from the Saccharomyces cerevisiae genome. The resulting pdc1(0) mutants were able to grow on glucose and still contained 60 to 70% of the wild-type PDC activity. Two DNA fragments with sequences homologous to that of the PDC1 gene were clo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.678-685.1990
更新日期:1990-02-01 00:00:00
abstract::The nucleotide sequence of the Clostridium thermocellum F1 xynC gene, which encodes the xylanase XynC, consists of 1,857 bp and encodes a protein of 619 amino acids with a molecular weight of 69,517. XynC contains a typical N-terminal signal peptide of 32 amino acid residues, followed by a 165-amino-acid sequence whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.13.4246-4253.1997
更新日期:1997-07-01 00:00:00
abstract::A procedure is described for the purification of the cores of flagella sheared from Vibrio cholerae. V. cholerae is a monotrichous organism whose flagellar core (FC) is enclosed within a sheath. The purification procedure consists of several cycles of differential centrifugation and cesium chloride density-gradient ul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.1121-1128.1977
更新日期:1977-02-01 00:00:00
abstract::The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00406-08
更新日期:2008-07-01 00:00:00
abstract::Glycerol was found to unravel the helical conformation of Escherichia coli type 1 fimbriae without appreciable depolymerization. The linearized fimbrial polymers have a diameter of 2 nm, react strongly with a monoclonal antibody directed at an inaccessible epitope on native fimbriae, and display greater mannose-bindin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.5145-5148.1992
更新日期:1992-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.2.530-540.1992
更新日期:1992-01-01 00:00:00
abstract::Two sets of genes for the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) were detected in the photosynthetic purple sulfur bacterium Chromatium vinosum by hybridization analysis with RuBisCO gene probes, cloned by using the lambda Fix vector, and designated rbcL-rbcS and rbcA-rbc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2391-2400.1989
更新日期:1989-05-01 00:00:00
abstract::Bergdoll, Merlin S. (University of Chicago, Chicago, Ill.), Concordia R. Borja, and Remedios M. Avena. Identification of a new enterotoxin as enterotoxin C. J. Bacteriol. 90:1481-1485. 1965.-Identification of a new enterotoxin was accomplished by purification of the enterotoxins produced by staphylococcal strains 137 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.5.1481-1485.1965
更新日期:1965-11-01 00:00:00
abstract::The regulatory system that controls the expression of the Ti plasmid-borne octopine degradation (uad) and transfer (tra) genes in Agrobacterium tumefaciens was studied. A deletion mutant derived from the cointegrate plasmid R702::Ti-B6S3 was isolated, which was compatible with a wild-type Ti plasmid and which had reta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.424-431.1979
更新日期:1979-08-01 00:00:00
abstract::The chromosomal DNA replication origins (oriC) from two members of the family Enterobacteriaceae, Enterobacter aerogenes and Klebsiella pneumoniae, have been isolated as functional replication origins in Escherichia coli. The origins in the SalI restriction fragments of 17.5 and 10.2 kilobase pairs, cloned from E. aer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::Studies on the active product of pepsin digestion of rabbit immunoglobulin G (IgG), F(ab')(2) produced from antiserum against Neurospora glutamate dehydrogenase, showed it to behave in the same way, in both antienzyme and precipitation experiments, as homologous IgG. It is proposed that the inhibition of glutamate deh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.958-962.1967
更新日期:1967-10-01 00:00:00
abstract::The TatA and TatB proteins are essential components of the twin arginine protein translocation pathway in Escherichia coli. C-terminal truncation analysis of the TatA protein revealed that a plasmid-expressed TatA protein shortened by 40 amino acids is still fully competent to support protein translocation. Similar tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.21.5871-5879.2002
更新日期:2002-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.17.5455-5460.1999
更新日期:1999-09-01 00:00:00
abstract::Under static growth conditions, hyperpiliated, nontwitching pilT and pilU mutants of Pseudomonas aeruginosa formed dense biofilms, showing that adhesion, not twitching motility, is necessary for biofilm initiation. Under flow conditions, the pilT mutant formed mushroom-like structures larger than those of the wild typ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.7.2374-2378.2003
更新日期:2003-04-01 00:00:00
abstract::In Escherichia coli, repression of the aceBAK operon is mediated by the IclR protein. We used an in vitro oligonucleotide selection technique to determine the consensus recognition sequence for MR. Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3982-3984.1996
更新日期:1996-07-01 00:00:00
abstract::Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio), and Dorothy J. Geason. Pattern of sheath synthesis in Sphaerotilus natans. J. Bacteriol. 88:1145-1150. 1964.-Formation of the characteristic sheath of Sphaerotilus natans was followed by immunofluorescence. Fluorescent antisheath antibody was obtained by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.4.1145-1150.1964
更新日期:1964-10-01 00:00:00
abstract::Antiserum prepared against protocatechuate 3, 4-dioxygenase from Pseudomonas aeruginosa forms precipitin bands without spurs with isofunctional enzymes from different strains of the fluorescent pseudomonads on immunodiffusion plates. Catalytic activity of the isofunctional enzymes was inhibited by an immunoglobulin fr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.516-519.1976
更新日期:1976-04-01 00:00:00
abstract::Mycobacterium avium-Mycobacterium intracellulare complex (MAC) is the most common isolate of nontuberculous mycobacteria and causes pulmonary and extrapulmonary diseases. MAC species can be grouped into 31 serotypes by the epitopic oligosaccharide structure of the species-specific glycopeptidolipid (GPL) antigen. The ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01850-07
更新日期:2008-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.735-736.1975
更新日期:1975-02-01 00:00:00
abstract::Neisseria meningitidis can be the causative agent of meningitis or septicemia. This bacterium expresses type IV pili, which mediate a variety of functions, including autoagglutination, twitching motility, biofilm formation, adherence, and DNA uptake during transformation. The secretin PilQ supports type IV pilus extru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00060-07
更新日期:2007-08-01 00:00:00
abstract::Streptococcus agalactiae is a primary cause of neonatal morbidity and mortality. Essential to the virulence of this pathogen is the production of a type-specific capsular polysaccharide (CPS) that enables the bacteria to evade host immune defenses. The identification, cloning, sequencing, and functional characterizati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.16.4466-4477.2000
更新日期:2000-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00698-08
更新日期:2008-12-01 00:00:00
abstract::The Gram-positive bacterium Streptococcus pyogenes (also called group A Streptococcus [GAS]), is found strictly in humans and is capable of causing a wide variety of infections. Here we demonstrate that serine catabolism in GAS is controlled by the transcriptional regulator Spy49_0126c. We have designated this regulat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01516-10
更新日期:2011-04-01 00:00:00
abstract::This study describes a novel approach to identify unique genomic DNA sequences from the unsequenced strain C. jejuni ATCC 43431 by comparison with the sequenced strain C. jejuni NCTC 11168. A shotgun DNA microarray was constructed by arraying 9,600 individual DNA fragments from a C. jejuni ATCC 43431 genomic library o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.14.4781-4795.2004
更新日期:2004-07-01 00:00:00
abstract::Most isolates of Salmonella enterica serovar Typhimurium contain a 90-kb virulence plasmid. This plasmid is reported to be mobilizable but nonconjugative. However, we have determined that the virulence plasmid of strains LT2, 14028, and SR-11 is indeed self-transmissible. The plasmid of strain SL1344 is not. Optimal c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.4.1364-1368.1999
更新日期:1999-02-01 00:00:00