Abstract:
:The nucleotide sequence of the Clostridium thermocellum F1 xynC gene, which encodes the xylanase XynC, consists of 1,857 bp and encodes a protein of 619 amino acids with a molecular weight of 69,517. XynC contains a typical N-terminal signal peptide of 32 amino acid residues, followed by a 165-amino-acid sequence which is homologous to the thermostabilizing domain. Downstream of this domain was a family 10 catalytic domain of glycosyl hydrolase. The C terminus separated from the catalytic domain by a short linker sequence contains a dockerin domain responsible for cellulosome assembly. The N-terminal amino acid sequence of XynC-II, the enzyme purified from a recombinant Escherichia coli strain, was in agreement with that deduced from the nucleotide sequence although XynC-II suffered from proteolytic truncation by a host protease(s) at the C-terminal region. Immunological and N-terminal amino acid sequence analyses disclosed that the full-length XynC is one of the major components of the C. thermocellum cellulosome. XynC-II was highly active toward xylan and slightly active toward p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-cellobioside, p-nitrophenyl-beta-D-glucopyranoside, and carboxymethyl cellulose. The Km and Vmax values for xylan were 3.9 mg/ml and 611 micromol/min/mg of protein, respectively. This enzyme was optimally active at 80 degrees C and was stable up to 70 degrees C at neutral pHs and over the pH range of 4 to 11 at 25 degrees C.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hayashi H,Takagi KI,Fukumura M,Kimura T,Karita S,Sakka K,Ohmiya Kdoi
10.1128/jb.179.13.4246-4253.1997subject
Has Abstractpub_date
1997-07-01 00:00:00pages
4246-53issue
13eissn
0021-9193issn
1098-5530journal_volume
179pub_type
杂志文章abstract::During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection a...
journal_title:Journal of bacteriology
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pub_type: 杂志文章
doi:10.1128/JB.94.6.1919-1923.1967
更新日期:1967-12-01 00:00:00
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更新日期:1975-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.3.1158-1161.1981
更新日期:1981-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1992-09-01 00:00:00
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doi:10.1128/JB.115.1.73-75.1973
更新日期:1973-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.100.2.1136-1137.1969
更新日期:1969-11-01 00:00:00
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
doi:10.1128/jb.184.7.2019-2029.2002
更新日期:2002-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.3.1438-1440.1972
更新日期:1972-12-01 00:00:00
abstract::Hottle, G. A. (Naval Biological Laboratory, University of California, Berkeley), and D. N. Wright. Growth and survival of Mycoplasma neurolyticum in liquid media. J. Bacteriol. 91:1834-1839. 1966.-Maximal growth of Mycoplasma neurolyticum (between 10(8) and 10(9) colony-forming units per ml) was obtained after 3 days ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1834-1839.1966
更新日期:1966-05-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:2007-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
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更新日期:2000-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1997-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.5.2755-2761.1990
更新日期:1990-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.10.2825-2835.1996
更新日期:1996-05-01 00:00:00
abstract::Expression of the 146-kilodalton (kDa) extracellular glucoamylase by the budding yeast Schwanniomyces castellii is induced by maltose and starch. By use of antiglucoamylase antisera, we found that this expression was regulated at the level of the mRNA, taking place within 30 min after exposure of yeast cells to the re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.5.2360-2366.1990
更新日期:1990-05-01 00:00:00
abstract::Growth rate control of adherent, sessile populations was achieved by the controlled perfusion of membrane-associated bacterial biofilms by the method of Gilbert et al. (P. Gilbert, D. G. Allison, D. J. Evans, P. S. Handley, and M. R. W. Brown, Appl. Environ. Microbiol. 55:1308-1311, 1989). Changes in cell surface hydr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1667-1669.1990
更新日期:1990-03-01 00:00:00
abstract::The Escherichia coli htrD gene was originally isolated during a search for new genes required for growth at high temperature. Insertional inactivation of htrD leads to a pleiotropic phenotype characterized by temperature-sensitive growth in rich medium, H2O2 sensitivity, and sensitivity to cysteine. The htrD gene was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.1.166-175.1993
更新日期:1993-01-01 00:00:00