Investigation of two evolutionarily unrelated halocarboxylic acid dehalogenase gene families.

Abstract:

:Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily unrelated and together represent almost all of the alphaHA deh genes described to date. We report the design and evaluation of degenerate PCR primer pairs for the separate amplification and isolation of group I and II deh genes. Amino acid sequences derived from 10 of 11 group I deh partial gene products of new and previously reported bacterial isolates showed conservation of five residues previously identified as essential for activity. The exception, DehD from a Rhizobium sp., had only two of these five residues. Group II deh gene sequences were amplified from 54 newly isolated strains, and seven of these sequences were cloned and fully characterized. Group II dehalogenases were stereoselective, dechlorinating L- but not D-2-chloropropionic acid, and derived amino acid sequences for all of the genes except dehII degrees P11 showed conservation of previously identified essential residues. Molecular analysis of the two deh families highlighted four subdivisions in each, which were supported by high bootstrap values in phylogenetic trees and by enzyme structure-function considerations. Group I deh genes included two putative cryptic or silent genes, dehI degrees PP3 and dehI degrees 17a, produced by different organisms. Group II deh genes included two cryptic genes and an active gene, dehIIPP3, that can be switched off and on. All alphaHA-degrading bacteria so far described were Proteobacteria, a result that may be explained by limitations either in the host range for deh genes or in isolation methods.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hill KE,Marchesi JR,Weightman AJ

doi

10.1128/JB.181.8.2535-2547.1999

keywords:

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

2535-47

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

杂志文章
  • "Position effect" on dominance in the D-serine deaminase system of Escherichia coli K-12.

    abstract::A completely stable F32 merodiploid has been isolated, which has the dsd genotype dsdA(+)dsdCx3 /F -dsdA(+)dsdC(+). The stabilization apparently does not result from an alteration in the F, or from its integration in the chromosome, but rather from a chromosomal aberration. The stable strain can exist in two forms, wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.6.1989-1993.1967

    authors: McFall E

    更新日期:1967-12-01 00:00:00

  • Glutathione plays a fundamental role in growth and symbiotic capacity of Sinorhizobium meliloti.

    abstract::Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined the importance of glutathione (GSH) during free-living growth and symbiosis of Sinorhizobium meliloti. An S. meliloti mutant strain (SmgshA) which is unab...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.1.168-174.2005

    authors: Harrison J,Jamet A,Muglia CI,Van de Sype G,Aguilar OM,Puppo A,Frendo P

    更新日期:2005-01-01 00:00:00

  • Identification of crucial amino acids of bacterial Peptide deformylases affecting enzymatic activity in response to oxidative stress.

    abstract::Peptide deformylase (PDF) is an essential bacterial metalloprotease involved in deformylation of N-formyl group from nascent polypeptide chains during protein synthesis. Iron-containing variants of this enzyme from Salmonella enterica serovar Typhimurium (sPDF) and Mycobacterium tuberculosis (mPDF), although inhibited...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00916-13

    authors: Kumar S,Kanudia P,Karthikeyan S,Chakraborti PK

    更新日期:2014-01-01 00:00:00

  • Products of phospholipid metabolism in Bacillus stearothermophilus.

    abstract::The products of phospholipid turnover in Bacillus stearothermophilus were determined in cultures labeled to equilibrium and with short pulses of [32P]phosphate and [2-3H]glycerol. Label lost from the cellular lipid pool was recovered in three fractions: low-molecular-weight extracellular products, extracellular lipid,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.1.294-303.1983

    authors: Card GL,Finn DJ

    更新日期:1983-04-01 00:00:00

  • Extracellular loops of lipid A 3-O-deacylase PagL are involved in recognition of aminoarabinose-based membrane modifications in Salmonella enterica serovar typhimurium.

    abstract::Salmonella enterica serovar Typhimurium modifies its lipopolysaccharide (LPS), including the lipid A portion, in response to changes in its environment including host tissues. The lipid A 3-O-deacylase PagL, the expression of which is promoted under a host-mimetic environment, exhibits latency in S. enterica; deacylat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00587-08

    authors: Manabe T,Kawasaki K

    更新日期:2008-08-01 00:00:00

  • Extracellular hemolysins of aerobic sporogenic bacilli.

    abstract::Forty-five strains, representing 18 species of the genus Bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. Broth cultures of B. cereus, B. alvei, and B. laterosporus contained lysins that closely resembled streptolysin O. B. subtilis and a single strain of B. cereus may produce lysins ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.5.1541-1543.1967

    authors: Bernheimer AW,Grushoff P

    更新日期:1967-05-01 00:00:00

  • Evidence for the Translocation of a Chromosome Sement in Bacillus subtilis Strains Carrying the trpE26 Mutation.

    abstract::The replication order of markers was studied in Bacillus subtilis strains bearing the trpE26 mutation by the use of the density transfer technique. An important difference in this order was observed in comparison with that of strain 168 T-. All markers tested of a chromosome segment extending from trpD to ilvA replica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.886-898.1975

    authors: Trowsdale J,Anagnostopoulos C

    更新日期:1975-06-01 00:00:00

  • Formation of 9-nm filaments from pilin monomers obtained by octyl-glucoside dissociation of Pseudomonas aeruginosa pili.

    abstract::Pili isolated from Pseudomonas aeruginosa PAK were solubilized in the detergent octyl-glucoside. Subsequent removal of the detergent by dialysis resulted in the formation of short rods of about 9 nm in diameter and various lengths. The aggregation process was followed by ultracentrifugation, viscometry, and electron m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.3.1508-1513.1982

    authors: Watts TH,Scraba DG,Paranchych W

    更新日期:1982-09-01 00:00:00

  • Inhibition of Escherichia coli acetyl coenzyme A carboxylase by acyl-acyl carrier protein.

    abstract::Escherichia coli acetyl coenzyme A carboxylase (ACC), the first enzyme of the fatty acid biosynthetic pathway, is inhibited by acylated derivatives of acyl carrier protein (ACP). ACP lacking an acyl moiety does not inhibit ACC. Acylated derivatives of ACP having chain lengths of 6 to 20 carbon atoms were similarly inh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.4.1499-1503.2001

    authors: Davis MS,Cronan JE Jr

    更新日期:2001-02-01 00:00:00

  • Alpha-alpha'-dipyridyl or ortho-phenanthroline stimulation of the soluble reduced nicotinamide adenine dinucleotide oxidase from Bacillus subtilis spores and dipicolinic acid inhibition of the stimulated enzymes.

    abstract::Soluble reduced nicotinamide adenine dinucleotide oxidase activity in extracts of Bacillus subtilis spores was stimulated by the addition of not only flavine mononucleotide (FMN) or flavine adenine dinucleotide (FAD) but also alpha-alpha'-dipyridyl or o-phenanthroline. These chelating agents showed stronger effect on ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.3.1017-1022.1974

    authors: Tochikubo K

    更新日期:1974-03-01 00:00:00

  • Polyribosomes from Bacillus subtilis during amino acid starvation in the presence and in the absence of actinomycin.

    abstract::Polysomes were extracted from Bacillus subtilis cells starved for a required amino acid. The monosome peak appeared soon after starvation; no difference in the rate of degradation was detected when the cells were starved for arginine or tryptophan in a double auxotroph. RNA production during starvation was not inhibit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.3.925-930.1970

    authors: Barlati S

    更新日期:1970-03-01 00:00:00

  • Membrane topology and DNA-binding ability of the Streptococcal CpsA protein.

    abstract::Many streptococcal pathogens require a polysaccharide capsule for survival in the host during systemic infection. The highly conserved CpsA protein is proposed to be a transcriptional regulator of capsule production in streptococci, although the regulatory mechanism is unknown. Hydropathy plots of CpsA predict an inte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01098-10

    authors: Hanson BR,Lowe BA,Neely MN

    更新日期:2011-01-01 00:00:00

  • Control of the Staphylococcus aureus toxic shock tst promoter by the global regulator SarA.

    abstract::The Staphylococcus aureus SarA global regulator controls the expression of numerous virulence genes, often in conjunction with the agr quorum-sensing system and its effector RNA, RNAIII. In the present study, we have examined the role of both SarA and RNAIII on the regulation of the promoter of tst, encoding staphyloc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00146-10

    authors: Andrey DO,Renzoni A,Monod A,Lew DP,Cheung AL,Kelley WL

    更新日期:2010-11-01 00:00:00

  • ENZYME ASSOCIATED WITH BACTERIOPHAGE INFECTION.

    abstract::Eklund, Curtis (The University of Texas, Austin) and Orville Wyss. Enzyme associated with bacteriophage infection. J. Bacteriol. 84:1209-1215. 1962.-A capsule-digesting enzyme was formed when azotobacter cells were infected with bacteriophage. The enzyme appeared in the medium when the phages lysed the cells. By disru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.6.1209-1215.1962

    authors: Eklund C,Wyss O

    更新日期:1962-12-01 00:00:00

  • Induction of colicin 1a at high temperature.

    abstract::Induction of colicin la at a high temperature (42 C) was demonstrated in a wild strain of Shigella sonnei. After transfer of the Col factor, a similar effect was observed in those recipient cells acquiring colicinogeny. The induction is suggested to be due to the presence of a thermosensitive colicin repressor. The wi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.3.720-723.1971

    authors: Gromkova RH

    更新日期:1971-06-01 00:00:00

  • Genetic circularity of the Pseudomonas aeruginosa PAO chromosome.

    abstract::Genetic circularity of the Pseudomonas aeruginosa PAO chromosome was demonstrated by a series of two- and three-factor crosses and double-selection experiments with Cma plasmids FP2, FP5, FP110, and R68.45. A range of additional markers, including catabolic markers, were located on the chromosome map. Plasmid FP2, kno...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.1.145-155.1981

    authors: Royle PL,Matsumoto H,Holloway BW

    更新日期:1981-01-01 00:00:00

  • Glutamine synthetase mutations which affect expression of nitrogen fixation genes in Klebsiella pneumoniae.

    abstract::Previous studies have implicated glutamine synthetase (L-glutamate:ammonia ligase [adenosine diphosphate for-ing], EC 6.6.1.2) as a major controlling element of the nitrogen fixation (nif) genes in Klebsiella pneumoniae. We report here the isolation of a new class of K. pneumoniae mutants which exhibit altered pattern...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.597-606.1979

    authors: Ausubel FM,Bird SC,Durbin KJ,Janssen KA,Margolskee RF,Peskin AP

    更新日期:1979-11-01 00:00:00

  • Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.

    abstract::A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Elseviers D,Gallagher P,Hoffman A,Weinberg B,Schwartz I

    更新日期:1982-10-01 00:00:00

  • Superoxide dismutase and catalase levels in halophilic vibrios.

    abstract::Superoxide dismutase (SOD) and catalase (CAT) levels were determined for several aerobically grown halophilic vibrios and compared with those found in aerobically grown Escherichia coli K-12. The SOD levels ranged from 25 to 103.6 U/mg of protein for the vibrios compared with 44.6 U/mg of protein for E. coli. The CAT ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.2.375-380.1978

    authors: Daily OP,Debell RM,Joseph SW

    更新日期:1978-05-01 00:00:00

  • Accessory gene regulator control of staphyloccoccal enterotoxin d gene expression.

    abstract::The quorum-sensing system of Staphylococcus aureus, the accessory gene regulator (Agr) system, is responsible for increased transcription of certain exoprotein genes and decreased transcription of certain cell wall-associated proteins during the postexponential phase of growth. This regulation is important for virulen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.6.1793-1801.2004

    authors: Tseng CW,Zhang S,Stewart GC

    更新日期:2004-03-01 00:00:00

  • Site-specific recombination system encoded by toluene catabolic transposon Tn4651.

    abstract::The 56-kb class II toluene catabolic transposon Tn4651 from Pseudomonas putida plasmid pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution (res) site, the two gene products TnpS and TnpT and (ii) the 2.4-kb tnpT-res-tnpS region is 48 kb apart from the tnpA gene (M. Tsuda,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.17.4757-4766.2002

    authors: Genka H,Nagata Y,Tsuda M

    更新日期:2002-09-01 00:00:00

  • Mechanical properties of Bacillus subtilis cell walls: effects of removing residual culture medium.

    abstract::Experiments are described in which the tensile strength, the initial (Youngs') modulus, and other mechanical properties of the bacterial cell wall were obtained as functions of relative humidity (RH) in the range of 20 to 95%. These properties were deduced from tensile tests on bacterial thread, a fiber consisting of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.1.197-203.1991

    authors: Thwaites JJ,Surana UC

    更新日期:1991-01-01 00:00:00

  • Effect of aeration and sodium on the metabolism of citrate by Klebsiella aerogenes.

    abstract::Anaerobic growth of Klebsiella aerogenes NCDO 711 (NCTC 418) on citrate was dependent on the presence of Na+ in the medium, and fermentation of citrate was mediated via the fermentation pathway enzymes, citrate lyase and a Na+-dependent oxalacetate decarboxylase. This confirms the previous findings on strain NCTC 418....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.2.468-473.1975

    authors: O'Brien RW

    更新日期:1975-05-01 00:00:00

  • Essential role of flavohemoglobin in long-term anaerobic survival of Bacillus subtilis.

    abstract::A Bacillus subtilis culture incubated anaerobically in nitrate-containing medium lost viability during the first 3 days but recovered thereafter. A flavohemoglobin mutant showed very poor survival under these conditions unless the cells were prevented from carrying out nitrate respiration. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00557-06

    authors: Nakano MM

    更新日期:2006-09-01 00:00:00

  • Suppression of ftsH mutant phenotypes by overproduction of molecular chaperones.

    abstract::Decreased intracellular levels of FtsH, a membrane-bound ATPase, led to retardation of growth and protein export, as well as to an abnormal translocation of alkaline phosphatase that had been attached to a cytoplasmic domain of a multispanning membrane protein, SecY. The last phenotype is designated Std (stop transfer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.4.1141-1145.1996

    authors: Shirai Y,Akiyama Y,Ito K

    更新日期:1996-02-01 00:00:00

  • The Sakaguchi reaction product quenches phycobilisome fluorescence, allowing determination of the arginine concentration in cells of Anabaena strain PCC 7120.

    abstract::The filamentous cyanobacterium Anabaena fixes nitrogen in specialized cells called heterocysts. The immediate product of fixation, ammonia, is known to be assimilated by addition to glutamate to make glutamine. How fixed nitrogen is transported along the filament to the 10 to 20 vegetative cells that separate heterocy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01512-12

    authors: Ke S,Haselkorn R

    更新日期:2013-01-01 00:00:00

  • Complete genome sequence of "Vulcanisaeta moutnovskia" strain 768-28, a novel member of the hyperthermophilic crenarchaeal genus Vulcanisaeta.

    abstract::Strain 768-28 was isolated from a hot spring in Kamchatka, Russia, and represents a novel member of the Vulcanisaeta genus. The complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the Embden-Meyerhof and Entner-Doudoroff pathways for gluc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00237-11

    authors: Gumerov VM,Mardanov AV,Beletsky AV,Prokofeva MI,Bonch-Osmolovskaya EA,Ravin NV,Skryabin KG

    更新日期:2011-05-01 00:00:00

  • Cloning, sequences, and characterization of two chitinase genes from the Antarctic Arthrobacter sp. strain TAD20: isolation and partial characterization of the enzymes.

    abstract::Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.5.1773-1779.2001

    authors: Lonhienne T,Mavromatis K,Vorgias CE,Buchon L,Gerday C,Bouriotis V

    更新日期:2001-03-01 00:00:00

  • The serotype of type Ia and III group B streptococci is determined by the polymerase gene within the polycistronic capsule operon.

    abstract::Streptococcus agalactiae is a primary cause of neonatal morbidity and mortality. Essential to the virulence of this pathogen is the production of a type-specific capsular polysaccharide (CPS) that enables the bacteria to evade host immune defenses. The identification, cloning, sequencing, and functional characterizati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.16.4466-4477.2000

    authors: Chaffin DO,Beres SB,Yim HH,Rubens CE

    更新日期:2000-08-01 00:00:00

  • BamE modulates the Escherichia coli beta-barrel assembly machine component BamA.

    abstract::Biogenesis of the outer membrane (OM) is an essential process in gram-negative bacteria. One of the key steps of OM biogenesis is the assembly of integral outer membrane beta-barrel proteins (OMPs) by a protein machine called the Bam complex. In Escherichia coli, the Bam complex is composed of the essential proteins B...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06426-11

    authors: Rigel NW,Schwalm J,Ricci DP,Silhavy TJ

    更新日期:2012-03-01 00:00:00