Glutamine synthetase mutations which affect expression of nitrogen fixation genes in Klebsiella pneumoniae.

Abstract:

:Previous studies have implicated glutamine synthetase (L-glutamate:ammonia ligase [adenosine diphosphate for-ing], EC 6.6.1.2) as a major controlling element of the nitrogen fixation (nif) genes in Klebsiella pneumoniae. We report here the isolation of a new class of K. pneumoniae mutants which exhibit altered patterns of nif and hut (histidine utlization) regulation. The expression of nif in these mutants, which were isolated as Gln+ (glutamine nonrequiring) revertants of a particular glnA mutation, is extremely sensitive to ammonia repression. These mutants have a Nif- Hut- phenotype at external ammonia concentrations at which wild-type strains are Nif+ Hut+. On the other hand, these mutants can be fully derepressed for nif at very low ammonia concentrations. We adopted the nomenclature "GlnR- (Nif- Hut-)" to facilitate discussion of the phenotype of these mutant strains. The mutations in these strains which confer the GlnR- phenotype map at or near glnA, the structural gene for glutamine synthetase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ausubel FM,Bird SC,Durbin KJ,Janssen KA,Margolskee RF,Peskin AP

doi

10.1128/JB.140.2.597-606.1979

subject

Has Abstract

pub_date

1979-11-01 00:00:00

pages

597-606

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

140

pub_type

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