Abstract:
:The aim of this study was to identify the compatible solutes accumulated by Pseudomonas putida S12 subjected to osmotic stress. In response to reduced water activity, P. putida S12 accumulated Nalpha-acetylglutaminylglutamine amide (NAGGN) simultaneously with a novel compatible solute identified as mannitol (using 13C- and 1H-nuclear magnetic resonance, liquid chromatography-mass spectroscopy and high-performance liquid chromatography methods) to maximum concentrations of 74 and 258 micromol g (dry weight) of cells(-1), respectively. The intracellular amounts of each solute varied with both the type and amount of osmolyte applied to induce osmotic stress in the medium. Both solutes were synthesized de novo. Addition of betaine to the medium resulted in accumulation of this compound and depletion of both NAGGN and mannitol. Mannitol and NAGGN were accumulated when sucrose instead of salts was used to reduce the medium water activity. Furthermore, both compatible solutes were accumulated when glucose was substituted by other carbon sources. However, the intracellular quantities of mannitol decreased when fructose, succinate, or lactate were applied as a carbon source. Mannitol was also raised to high intracellular concentrations by other salt-stressed Pseudomonas putida strains. This is the first study demonstrating a principal role for the de novo-synthesized polyol mannitol in osmoadaptation of a heterotrophic eubacterium.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kets EP,Galinski EA,de Wit M,de Bont JA,Heipieper HJdoi
10.1128/jb.178.23.6665-6670.1996subject
Has Abstractpub_date
1996-12-01 00:00:00pages
6665-70issue
23eissn
0021-9193issn
1098-5530journal_volume
178pub_type
杂志文章abstract::The glmU gene product of Escherichia coli was recently identified as the N-acetylglucosamine-1-phosphate uridyltransferase activity which catalyzes the formation of UDP-N-acetylglucosamine, an essential precursor for cell wall peptidoglycan and lipopolysaccharide biosyntheses (D. Mengin-Lecreulx and J. van Heijenoort,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5788-5795.1994
更新日期:1994-09-01 00:00:00
abstract::In Escherichia coli, pyrimidine-mediated regulation of upp expression occurs by UTP-sensitive selection of alternative transcriptional start sites, which produces transcripts that differ in the ability to be elongated. The upp initially transcribed region contains the sequence GATTTTTTTTG (nontemplate strand). Initiat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.1.221-228.2001
更新日期:2001-01-01 00:00:00
abstract::Legionella pneumophila expresses two peroxide-scavenging alkyl hydroperoxide reductase systems (AhpC1 and AhpC2D) that are expressed differentially during the bacterial growth cycle. Functional loss of the postexponentially expressed AhpC1 system is compensated for by increased expression of the exponentially expresse...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00141-08
更新日期:2008-05-01 00:00:00
abstract::In Salmonella enterica, the biosynthetic pathways for the generation of purines and the essential cofactor thiamine pyrophosphate branch after sharing five enzymatic steps. Phosphoribosyl amine (PRA) is the first intermediate in the common portion of the pathway and is generated from phosphoribosylpyrophosphate and gl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00745-06
更新日期:2006-10-01 00:00:00
abstract::The location of the Escherichia coli K-12 genes determining or regulating glutamate transport, and the location of the gene determining glutamate decarboxylase synthesis, were established by conjugation. The ability to grow on glutamate as the sole source of carbon and energy was used to select for glutamate transport...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.4.1409-1415.1967
更新日期:1967-04-01 00:00:00
abstract::Lactobacillus casei and Streptococcus faecalis accumulated labeled folic acid and metabolized this compound to poly-gamma-glutamates of chain lengths of up to 11 and 5, respectively. Octa- and nonaglutamates predominated in L. casei, and tetraglutamates predominated in S. faecalis. The most effective monoglutamate sub...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.316-325.1983
更新日期:1983-01-01 00:00:00
abstract::The invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium is mediated by a type III secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). Expression of the SPI1 T3SS is tightly regulated by the combined action of HilC, HilD, and RtsA, three AraC family members that c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00926-07
更新日期:2008-01-01 00:00:00
abstract::Protein 1, a major protein of the outer membrane of Escherichia coli, has been shown to be the pore allowing the passage of small hydrophilic solutes across the outer membrane. In E. coli K-12 protein 1 consists of two subspecies, 1a and 1b, whereas in E. coli B it consists of a single species which has an electrophor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.3.1181-1189.1978
更新日期:1978-03-01 00:00:00
abstract::Nosocomial infections caused by antibiotic-resistant Klebsiella pneumoniae are emerging as a major health problem worldwide, while community-acquired K. pneumoniae infections present with a range of diverse clinical pictures in different geographic areas. In particular, an invasive form of K. pneumoniae that causes li...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00315-09
更新日期:2009-07-01 00:00:00
abstract::Perry, Jerome J. (North Carolina State University, Raleigh), and James B. Evans. Oxidation and assimilation of carbohydrates by Micrococcus sodonensis. J. Bacteriol. 91:33-38. 1966.-Micrococcus sodonensis is a biotin-requiring strict aerobe that cannot utilize carbohydrates as sole sources of carbon and energy. Howeve...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.33-38.1966
更新日期:1966-01-01 00:00:00
abstract::Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.852-854.1985
更新日期:1985-05-01 00:00:00
abstract::Raps, Shirley (University of Illinois, Urbana) and R. D. DeMoss. Glycolytic enzymes in Zymomonas mobilis. J. Bacteriol. 84:115-118. 1962-An enzyme extract of Zymomonas mobilis (Pseudomonas lindneri) was capable of fermenting glucose-6-phosphate to CO(2) and ethanol. The extract was found to contain phosphohexoisomeras...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.115-118.1962
更新日期:1962-07-01 00:00:00
abstract::Using transposon Tn5-mediated mutagenesis, an essential Rhizobium meliloti nitrogen fixation (nif) gene was identified and located directly downstream of the regulatory gene nifA. Maxicell and DNA sequence analysis demonstrated that the new gene is transcribed in the same direction as nifA and codes for a 54-kilodalto...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1120-1126.1987
更新日期:1987-03-01 00:00:00
abstract::Clostridium perfringens causes gas gangrene and gastrointestinal (GI) diseases in humans. The most common cause of C. perfringens-associated food poisoning is the consumption of C. perfringens vegetative cells followed by sporulation and production of enterotoxin in the gut. Despite the importance of spore formation i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02152-12
更新日期:2013-06-01 00:00:00
abstract::Macrocysts have been found among strains of Dictyostelium rosarium Raper and Cavender (K. B. Raper and J. C. Cavender, J. Elisha Mitchell Sci. Soc. 84:31-47, 1968). Strains that exhibit mating capabilities could be classified into one of three mating types: A1, A2, or A3. Each mating type was self-incompatible but cro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.3.1049-1053.1981
更新日期:1981-09-01 00:00:00
abstract::Fast-growing species of Rhizobium were found to secrete low-molecular-weight beta-2-linked glucans when cultured in synthetic liquid medium. These glucans are quite similar to beta-2-linked glucans produced by species of Agrobacterium. No reducing terminus was detected in these glucans. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.1.243-248.1980
更新日期:1980-04-01 00:00:00
abstract::Myxobacterial hemagglutinin (MBHA) is a major developmentally induced protein that accumulates during the period of cellular aggregation in the bacterium Myxococcus xanthus. It has been shown that this lectin is targeted to the cell surface and periplasmic space of developmental cells, suggesting that it may play a ro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3801-3808.1987
更新日期:1987-08-01 00:00:00
abstract::Isoaspartyl sites, in which an aspartic acid residue is linked to its C-flanking neighbor via its beta-carboxyl side chain, are generally assumed to be an abnormal modification arising as proteins age. The enzyme protein L-isoaspartate methyltransferase (PIMT), present in many bacteria, plants, and animals, catalyzes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.9.2872-2877.1999
更新日期:1999-05-01 00:00:00
abstract::Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.1.262-265.1972
更新日期:1972-01-01 00:00:00
abstract::Bacteria of the genus Prosthecobacter express homologs of eukaryotic α- and β-tubulin, called BtubA and BtubB (BtubA/B), that have been observed to assemble into filaments in the presence of GTP. BtubA/B polymers are proposed to be composed in vitro by two to six protofilaments in contrast to that in vivo, where they ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00211-17
更新日期:2017-09-05 00:00:00
abstract::An NADPH-specific disulfide reductase that is active with bis-gamma-glutamylcystine has been purified 1,900-fold from Halobacterium halobium to yield a homogeneous preparation of the enzyme. Purification of this novel reductase, designated bis-gamma-glutamylcystine reductase (GCR), and purification of halobacterial di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3459-3467.1988
更新日期:1988-08-01 00:00:00
abstract::Desulfurococcus kamchatkensis is an anaerobic organotrophic hyperthermophilic crenarchaeon isolated from a terrestrial hot spring. Its genome consists of a single circular chromosome of 1,365,223 bp with no extrachromosomal elements. A total of 1,474 protein-encoding genes were annotated, among which 205 are exclusive...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01525-08
更新日期:2009-04-01 00:00:00
abstract::An in vitro constructed plasmid, pVH15, consisting of the entire genome of the plasmid ColE1, the tryptophan operon of Escherichia coli, and regions of the bacteriophage PHI80pt190, spontaneously gave rise in E. coli to a mini-ColE1 plasmid consisting of approximately one-half of the ColE1 genome and a small segment o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.447-453.1976
更新日期:1976-04-01 00:00:00
abstract::Tyrosine-mediated repression of aroF and tyrP was studied by inserting DNA sequences between the two adjacent TYR R boxes which, in each case, overlap the respective RNA polymerase binding sites of these genes. In both cases, repression was greatest when homologous regions of these two TYR R boxes were on the same fac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.16.5079-5085.1991
更新日期:1991-08-01 00:00:00
abstract::A modified 3-hydroxypropionate cycle has been proposed as the autotrophic CO2 fixation pathway for the thermoacidophilic crenarchaeon Metallosphaera sedula. The cycle requires the reductive conversion of 3-hydroxypropionate to propionyl-coenzyme A (propionyl-CoA). The specific activity of the 3-hydroxypropionate-, CoA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01593-07
更新日期:2008-02-01 00:00:00
abstract::The exopolysaccharide of Pseudomonas marginalis HT041B has been characterized as a 1,3-linked galactoglucan in which galactose and glucose are in the alpha- and beta-anomeric configurations, respectively. The polysaccharide is substituted with pyruvate at the 4 and 6 positions of galactose and with succinic acid at ei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1760-1762.1989
更新日期:1989-03-01 00:00:00
abstract::High-resolution electron microscopy of polarly flagellated bacteria revealed that their flagella originate at a circular, differentiated portion of the cytoplasmic membrane approximately 25 nm in diameter. The flagella also have discs attaching them to the cell wall. These attachment discs are extremely resistant to l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.512-521.1969
更新日期:1969-10-01 00:00:00
abstract::Biofilm formation is a key factor in Vibrio cholerae environmental survival and host colonization. Production of biofilm enables V. cholerae to survive and persist in aquatic environments and aids in the passage through the gastric acid barrier to allow access to the small intestine. The genes involved in biofilm form...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00014-12
更新日期:2012-06-01 00:00:00
abstract::The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5789-5794.1990
更新日期:1990-10-01 00:00:00
abstract::Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3620-3630.1990
更新日期:1990-07-01 00:00:00