Cloning and sequencing of a Bacteroides ruminicola B(1)4 endoglucanase gene.

abstract：:Atomic force microscopy was used to measure the thickness of air-dried, collapsed murein sacculi from Escherichia coli K-12 and Pseudomonas aeruginosa PAO1. Air-dried sacculi from E. coli had a thickness of 3.0 nm, whereas those from P. aeruginosa were 1.5 nm thick. When rehydrated, the sacculi of both bacteria swelle...

journal_title：Journal of bacteriology

authors： Yao X,Jericho M,Pink D,Beveridge T

更新日期：1999-11-01 00:00:00

abstract：:We describe here the cloning of single EcoRI fragments from the tra region of F DNA using ColE1::Tn3 as vector. These plasmids, as well as the series of Skurray et al. (Proc. Natl. Acad. Sci. U.S.A. 73:64-68, 1976), have been used to refine the map positions of tra cistrons on the F factor as well as to define a new D...

journal_title：Journal of bacteriology

authors： Achtman M,Skurray RA,Thompson R,Helmuth R,Hall S,Beutin L,Clark AJ

更新日期：1978-03-01 00:00:00

abstract：:A mutant of Pseudomonas aeruginosa severely deficient in outer membrane protein F levels was isolated by screening heavily mutagenized strains for membrane protein alterations on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. To provide a basis for phenotypic comparison, three independent spontaneous reve...

journal_title：Journal of bacteriology

authors： Nicas TI,Hancock RE

更新日期：1983-01-01 00:00:00

abstract：:The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...

journal_title：Journal of bacteriology

authors： Tanous C,Chambellon E,Sepulchre AM,Yvon M

更新日期：2005-07-01 00:00:00

abstract：:A pLAFR3 cosmid clone designated pVir2 containing a 25-kilobase (kb) DNA insert was isolated from a wild-type Pseudomonas solanacearum GMI1000 genomic library. This cosmid was shown to complement all but one of the nine Tn5-induced mutants which have been isolated after random mutagenesis and which have lost both path...

journal_title：Journal of bacteriology

authors： Boucher CA,Van Gijsegem F,Barberis PA,Arlat M,Zischek C

更新日期：1987-12-01 00:00:00

abstract：:Mutans of Pseudomonas testosteroni were isolated for their inability to grow on m-hydroxybenzoate as sole carbon source. These mutants hydroxylated m-hydroxybenzoate for form 2,3-dihydroxybenzoate in high yeilds. The bioconversion described in this report represents the first reported example of 3-hydroxybenzoate 2-hy...

journal_title：Journal of bacteriology

authors： Daumy GO,McColl AS,Andrews GC

更新日期：1980-01-01 00:00:00

abstract：:Short-term labeling with radioactive thymidine led to inaccurate estimates of deoxyribonucleic acid synthesis at some growth rates in steady-state cultures of Escherichia coli B/r. Estimates were corrected in chemostat cultures by adding adenosine, a known inhibitor of thymidine phosphorylase. ...

journal_title：Journal of bacteriology

authors： Kubitschek HE,Valdez D,Freedman ML

更新日期：1972-06-01 00:00:00

abstract：:Cell walls of 19 Micromonospora species were analyzed for their components. All the cell walls had xylose and arabinose, but the presence of glucose, galactose, mannose, or rhamnose depended on the strain. Amino acids present in the walls consisted of glycine, glutamic acid, diaminopimelic acid, and alanine, in a mola...

journal_title：Journal of bacteriology

authors： Kawamoto I,Oka T,Nara T

更新日期：1981-05-01 00:00:00

abstract：:Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...

journal_title：Journal of bacteriology

authors： Heidinger S,Braun V,Pecoraro VL,Raymond KN

更新日期：1983-01-01 00:00:00

abstract：:Mutants of Escherichia coli K-12 constitutive for fatty acid degradation (fadR) showed an increased rate of utilization of exogenous acetate. Acetate transport, oxidation, and incorporation into macromolecules was approximately fivefold greater in fadR mutants than fadR+ strains during growth on succinate as a carbon ...

journal_title：Journal of bacteriology

authors： Maloy SR,Nunn WD

更新日期：1981-10-01 00:00:00

abstract：:Overproduction of tryptophanyl-tRNA synthetase increased trp operon expression by reducing transcription termination at the trp attenuator. The total cellular level of charged tRNATrp was not affected by increased levels of the synthetase. We propose that excess synthetase binds charged tRNATrp and reduces the concent...

journal_title：Journal of bacteriology

authors： Das A,Yanofsky C

更新日期：1984-11-01 00:00:00

abstract：:The lic3 locus of Haemophilus influenzae consists of four open reading frames. The derived amino acid sequences of orf2 and orf4 exhibit homology to Escherichia coli GalE and AdK, respectively. The functions of orf1 and orf3 remain unknown. orf1 contains multiple tandem repeats of the tetrameric DNA sequence CAAT near...

journal_title：Journal of bacteriology

authors： Szabo M,Maskell D,Butler P,Love J,Moxon R

更新日期：1992-11-01 00:00:00

abstract：:The direct binding of Streptococcus mitis to human platelets is mediated in part by two proteins (PblA and PblB) encoded by a lysogenic bacteriophage (SM1). Since SM1 is the first prophage of S. mitis that has been identified and because of the possible role of these phage-encoded proteins in virulence, we sought to c...

journal_title：Journal of bacteriology

authors： Siboo IR,Bensing BA,Sullam PM

更新日期：2003-12-01 00:00:00

abstract：:In Escherichia coli, the GlpT transporter, a member of the major facilitator superfamily, moves external glycerol 3-phosphate (G3P) into the cytoplasm in exchange for cytoplasmic phosphate. Study of intact cells showed that both GlpT and HisGlpT, a variant with an N-terminal six-histidine tag, are inhibited (50% inhib...

journal_title：Journal of bacteriology

authors： Fann MC,Busch A,Maloney PC

更新日期：2003-07-01 00:00:00

abstract：:We have cloned a lytic enzyme, PlyPH, with a specific lytic effect on Bacillus anthracis strains. PlyPH remains active between pH 4 and 10.5, and a single dose rescued a significant percentage of mice infected intraperitoneally with an attenuated B. anthracis strain. We propose PlyPH as a novel therapeutic agent. ...

journal_title：Journal of bacteriology

authors： Yoong P,Schuch R,Nelson D,Fischetti VA

更新日期：2006-04-01 00:00:00

abstract：:The origin of replication of Bacillus subtilis 168 trp thy dna-1 (temperature-sensitive initiation mutant) was labeled with [3H]thymidine. Analysis of labeled cells by autoradiography revealed that most of the radioactivity was associated with cell pole areas. To label the terminus, cells that had initiated were treat...

journal_title：Journal of bacteriology

authors： Sonnenfeld EM,Koch AL,Doyle RJ

更新日期：1985-09-01 00:00:00

abstract：:FtsK is essential for Escherichia coli cell division. We report that cells lacking the C terminus of FtsK are defective in chromosome segregation as well as septation, often exhibiting asymmetrically positioned nucleoids and large anucleate regions. Combining the corresponding truncated ftsK gene with a mukB null muta...

journal_title：Journal of bacteriology

authors： Yu XC,Weihe EK,Margolin W

更新日期：1998-12-01 00:00:00

abstract：:Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria...

journal_title：Journal of bacteriology

authors： Puskas A,Greenberg EP,Kaplan S,Schaefer AL

更新日期：1997-12-01 00:00:00

abstract：:Expression of the Escherichia coli OmpC and OmpF outer membrane proteins is regulated by the osmolarity of the culture media. In contrast, expression of OmpC in Salmonella typhi is not influenced by osmolarity, while OmpF is regulated as in E. coli. To better understand the lack of osmoregulation of OmpC expression in...

journal_title：Journal of bacteriology

authors： Martínez-Flores I,Cano R,Bustamante VH,Calva E,Puente JL

更新日期：1999-01-01 00:00:00

abstract：:The mannans of Saccharomyces chevalieri, S. italicus, S. diastaticus, and S. carlsbergensis, were acetolyzed, and the fragments were separated by gel filtration. All gave similar acetolysis fingerprints, which were distinguished from S. cerevisiae by the presence of a pentasaccharide component in addition to the mono-...

journal_title：Journal of bacteriology

authors： Ballou CE,Lipke PN,Raschke WC

更新日期：1974-02-01 00:00:00

abstract：:The Alcaligenes eutrophus JMP134 plasmid pJP4 contains genes necessary for the complete degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 3-chlorobenzoic acid. tfdA encodes 2,4-D monooxygenase, the initial enzyme in the 2,4-D catabolic pathway. The tfdA locus has recently been localized to a region on pJP4 13 ...

journal_title：Journal of bacteriology

authors： Perkins EJ,Lurquin PF

更新日期：1988-12-01 00:00:00

abstract：:Mutations in carbohydrate-negative mutants of Pseudomonas aeruginosa PAO1 individually deficient in glucose 6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), or pyruvate carboxylase (pyc) were mapped on the chromosome by plasmid R68.45-mediated conjugation and by bacteriophage F116L-mediated trans...

journal_title：Journal of bacteriology

authors： Roehl RA,Feary TW,Phibbs PV Jr

更新日期：1983-12-01 00:00:00

abstract：:The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the r...

journal_title：Journal of bacteriology

authors： Hackstadt T,Todd WJ,Caldwell HD

更新日期：1985-01-01 00:00:00

abstract：UNLABELLED:Clostridium perfringens type D strains are usually associated with diseases of livestock, and their virulence requires the production of epsilon toxin (ETX). We previously showed (J. Li, S. Sayeed, S. Robertson, J. Chen, and B. A. McClane, PLoS Pathog 7:e1002429, 2011, http://dx.doi.org/10.1371/journal.ppat....

journal_title：Journal of bacteriology

authors： Li J,Freedman JC,McClane BA

更新日期：2015-10-01 00:00:00

abstract：:Histidine decarboxylases from Klebsiella planticola and Enterobacter aerogenes were purified to homogeneity and compared with the histidine decarboxylase from Morganella morganii. All three enzymes required pyridoxal 5'-phosphate as a coenzyme, showed optimal activity at pH 6.5, decarboxylated only histidine among the...

journal_title：Journal of bacteriology

authors： Guirard BM,Snell EE

更新日期：1987-09-01 00:00:00

abstract：:In streptomycete anthracycline biosynthetic gene clusters, small open reading frames are located just upstream of minimal polyketide synthase genes. aknX is such a gene found in the aklavinone-aclacinomycin biosynthetic gene cluster of Streptomyces galilaeus. In order to identify its function, the aknX gene was expres...

journal_title：Journal of bacteriology

authors： Chung JY,Fujii I,Harada S,Sankawa U,Ebizuka Y

更新日期：2002-11-01 00:00:00

abstract：:A strain of Neurospora crassa defective in amino acid transport can utilize a variety of amino acids for growth when readily metabolizable nitrogen is limiting. Growth is accompanied by the production of an extracellular deaminase that converts the amino acid to its respective keto acid plus equimolar quantities of ut...

journal_title：Journal of bacteriology

authors： DeBusk RM,Ogilvie S

更新日期：1984-08-01 00:00:00

abstract：:The iota(a) component (i(a)) of Clostridium perfringens ADP ribosylates nonmuscle beta/gamma actin and skeletal muscle alpha-actin. Replacement of Arg-295 in i(a) with alanine led to a complete loss of NAD(+)-glycohydrolase (NADase) and ADP-ribosyltransferase (ARTase); that of the residue with lysine caused a drastic ...

journal_title：Journal of bacteriology

authors： Nagahama M,Sakaguchi Y,Kobayashi K,Ochi S,Sakurai J

更新日期：2000-04-01 00:00:00

abstract：:Knaysi, Georges (Cornell University, Ithaca, N.Y.). Effect of temperature on the rate of germination in Bacillus cereus. J. Bacteriol. 87:619-622. 1964.-By use of the change of the spores from bright to dark, when observed in dark contrast with a phase microscope, as a criterion for the incipience (first stage) of ger...

journal_title：Journal of bacteriology

authors： KNAYSI G

更新日期：1964-03-01 00:00:00

abstract：:The nucleotide second messengers pppGpp and ppGpp [(p)ppGpp] are responsible for the global downregulation of transcription, translation, DNA replication, and growth rate that occurs during the stringent response. More recent studies suggest that (p)ppGpp is also an important effector in many nonstringent processes, i...

journal_title：Journal of bacteriology

authors： Ababneh QO,Herman JK

更新日期：2015-01-01 00:00:00