Pseudomonas aeruginosa outer membrane permeability: isolation of a porin protein F-deficient mutant.

abstract：:Mevalonate (MVA) metabolism provides the isoprenoids used in archaeal lipid biosynthesis. In synthesis of isopentenyl diphosphate, the classical MVA pathway involves decarboxylation of mevalonate diphosphate, while an alternate pathway has been proposed to involve decarboxylation of mevalonate monophosphate. To identi...

journal_title：Journal of bacteriology

authors： Vannice JC,Skaff DA,Keightley A,Addo JK,Wyckoff GJ,Miziorko HM

更新日期：2014-03-01 00:00:00

abstract：:The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and the nonhemolytic classical strain 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32P-labeled hly-specific DNA probe. Rest...

journal_title：Journal of bacteriology

authors： Goldberg SL,Murphy JR

更新日期：1985-04-01 00:00:00

abstract：:Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...

journal_title：Journal of bacteriology

authors： Zhao N,Bai Y,Zhao XQ,Yang ZY,Bai FW

更新日期：2012-12-01 00:00:00

abstract：:Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...

journal_title：Journal of bacteriology

authors： Wolfe AD,Cook TM,Hahn FE

更新日期：1971-12-01 00:00:00

abstract：:In Neurospora crassa, histidine starvation of histidine mutants resulted in derepression of histidine, tryptophan, and arginine biosynthetic enzymes. The same tripartite derepression occurred in wild-type strain 74A when it was grown in medium supplemented with 3-amino-1,2,4-triazole, an inhibitor of histidine biosynt...

journal_title：Journal of bacteriology

authors： Carsiotis M,Jones RF,Wesseling AC

更新日期：1974-09-01 00:00:00

abstract：:The interaction between endonuclease V, the cyclobutane pyrimidine dimer-specific N-glycosylase/abasic lyase from bacteriophage T4, and DNA was investigated by DNase I footprinting methods. The catalytically inactive mutant E23Q was found to interact with a smaller region of DNA at the abasic site analog, tetrahydrofu...

journal_title：Journal of bacteriology

authors： Latham KA,Manuel RC,Lloyd RS

更新日期：1995-09-01 00:00:00

abstract：:An incompetent mutant (4S Com(-)) does not release competence-inducing factor (CF) into the culture medium and is not infected with TP-1C phage deoxyribonucleic acid (DNA) unless CF is added to the transfection assay. A TP-1C phage-resistant mutant (4S Ton-r) releases relatively large amounts of CF into the culture me...

journal_title：Journal of bacteriology

authors： Streips UN,Welker NE

更新日期：1971-06-01 00:00:00

abstract：:A transformable strain of Bacillus subtilis 6160, a derivative of B. subtilis 168, produces three kinds of casein hydrolytic enzymes (alkaline protease, neutral protease, and esterase) in a culture medium. B. natto IAM 1212 produces 15 to 20 times as much total proteolytic activity as does B. subtilis. Extracellular p...

journal_title：Journal of bacteriology

authors： Uehara H,Yoneda Y,Yamane K,Maruo B

更新日期：1974-07-01 00:00:00

abstract：:Selenium is a constituent in Escherichia coli of the anaerobic enzyme formate dehydrogenase in the form of selenocysteine. Selenium is also present in the tRNA of E. coli in the modified base 5-methylaminomethyl-2-selenouracil (mnm5Se2U). The pathways of bacterial selenium metabolism are largely uncharacterized, and i...

journal_title：Journal of bacteriology

authors： Kramer GF,Ames BN

更新日期：1988-02-01 00:00:00

abstract：:Intact cells of Bacillus stearothermophilus PV72 revealed, after conventional thin-sectioning procedures, the typical cell wall profile of S-layer-carrying gram-positive eubacteria consisting of a ca. 10-nm-thick peptidoglycan-containing layer and a ca. 10-nm-thick S layer. Cell wall preparations obtained by breaking ...

journal_title：Journal of bacteriology

authors： Breitwieser A,Gruber K,Sleytr UB

更新日期：1992-12-01 00:00:00

abstract：:The delta subunit of RNA polymerase, RpoE, is widespread in low-G+C Gram-positive bacteria and is thought to play a role in enhancing transcriptional specificity by blocking RNA polymerase binding at weak promoter sites and stimulating RNA synthesis by accelerating core enzyme recycling. Despite the well-studied bioch...

journal_title：Journal of bacteriology

authors： Xue X,Tomasch J,Sztajer H,Wagner-Döbler I

更新日期：2010-10-01 00:00:00

abstract：:Ikeda, Y. (University of Tokyo, Tokyo, Japan) and T. Iijima. Heterocaryosis-like phenomenon in Bacillus subtilis. J. Bacteriol. 83:1025-1028. 1962.-When a mixture of methionine-dependent, histidine-dependent, streptomycin-resistant cells and tryptophan-dependent, streptomycin-sensitive cells of Bacillus subtilis K is ...

journal_title：Journal of bacteriology

authors： Ikeda Y,Iijima T

更新日期：1962-05-01 00:00:00

abstract：:We have sequenced the rightmost 1,700 base pairs of bacteriophage PRD1. This region encompasses the right early region and completes the sequence of all PRD1 early functions. We have also mapped the 5' initiation site of right early transcripts in vivo and in vitro. This has allowed us to assign gene XII to an open re...

journal_title：Journal of bacteriology

authors： Gerendasy D,Ito J

更新日期：1990-04-01 00:00:00

abstract：:Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...

journal_title：Journal of bacteriology

authors： Milunovic B,diCenzo GC,Morton RA,Finan TM

更新日期：2014-02-01 00:00:00

abstract：:Rubrerythrin was purified by multistep chromatography under anaerobic, reducing conditions from the hyperthermophilic archaeon Pyrococcus furiosus. It is a homodimer with a molecular mass of 39.2 kDa and contains 2.9 +/- 0.2 iron atoms per subunit. The purified protein had peroxidase activity at 85 degrees C using hyd...

journal_title：Journal of bacteriology

authors： Weinberg MV,Jenney FE Jr,Cui X,Adams MW

更新日期：2004-12-01 00:00:00

abstract：:The 3.1-Mb genome of an outbreak methicillin-resistant Staphylococcus aureus (MRSA) strain (TW20) contains evidence of recently acquired DNA, including two large regions (635 kb and 127 kb). The strain is resistant to a wide range of antibiotics, antiseptics, and heavy metals due to resistance genes encoded on mobile ...

journal_title：Journal of bacteriology

authors： Holden MT,Lindsay JA,Corton C,Quail MA,Cockfield JD,Pathak S,Batra R,Parkhill J,Bentley SD,Edgeworth JD

更新日期：2010-02-01 00:00:00

abstract：:Assays of CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (CGPTase) (EC 2.7.8.12) in membranes isolated from Bacillus subtilis 168 wild type and 11 strains bearing conditional lethal thermosensitive mutations in tagB, tagD, or tagF revealed that CGPTase deficiency was associated only with mutant tagF all...

journal_title：Journal of bacteriology

authors： Pooley HM,Abellan FX,Karamata D

更新日期：1992-01-01 00:00:00

abstract：:Wild-type Escherichia coli cells are unable to grow on beta-glucosides. Spontaneous mutants arise, however, which are able to utilize certain aromatic beta-glucosides such as salicin or arbutin as carbon sources, revealing the presence of a cryptic operon called bgl. Mutations activating the operon map within (or clos...

journal_title：Journal of bacteriology

authors： Schnetz K,Toloczyki C,Rak B

更新日期：1987-06-01 00:00:00

abstract：:To examine the role of the amino acid residues (between positions 258 and 275 and positions 297 and 298) of the alpha-subunit of RNA polymerase in TyrR-mediated activation of the mtr promoter, we have carried out in vitro transcription experiments using a set of mutant RNA polymerases with a supercoiled mtr template. ...

journal_title：Journal of bacteriology

authors： Yang J,Murakami K,Camakaris H,Fujita N,Ishihama A,Pittard AJ

更新日期：1997-10-01 00:00:00

abstract：:The physiological effects of incubation at nonpermissive temperatures of Escherichia coli mutants that carry a temperature-sensitive dnaZ allele [dnaZ(Ts)2016] were examined. The temperature at which the dnaZ(Ts) protein becomes inactivated in vivo was investigated by measurements of deoxyribonucleic acid (DNA) synthe...

journal_title：Journal of bacteriology

authors： Chu H,Malone MM,Haldenwang WG,Walker JR

更新日期：1977-10-01 00:00:00

abstract：:Escherichia coli K-12 produces at least two ATP-dependent proteases, Lon (La) and Clp (Ti), the latter consisting of a regulatory subunit (ClpA) and a proteolytic subunit (ClpP). The gene clpB encoding an analog of ClpA had been found at 57 min on the E. coli chromosome. Cloning and examination of novel heat shock pro...

journal_title：Journal of bacteriology

authors： Kitagawa M,Wada C,Yoshioka S,Yura T

更新日期：1991-07-01 00:00:00

abstract：:Competent cell-deoxyribonucleic acid (DNA) interactions were examined using tritium-labeled homologous or heterologous native or denatured DNAs and competent Streptococcus sanguis Wicky cells (strain WE4). The DNAs used were extracted from WE4 cells, Escherichia coli B cells, and E. coli bacteriophages T2, T4, T6, and...

journal_title：Journal of bacteriology

authors： Ranhand JM

更新日期：1980-05-01 00:00:00

abstract：:The invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium is mediated by a type III secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). Expression of the SPI1 T3SS is tightly regulated by the combined action of HilC, HilD, and RtsA, three AraC family members that c...

journal_title：Journal of bacteriology

authors： Ellermeier JR,Slauch JM

更新日期：2008-01-01 00:00:00

abstract：:At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The...

journal_title：Journal of bacteriology

authors： Dempsey WB,Ito H

更新日期：1970-11-01 00:00:00

abstract：:Penicillin-binding protein (PBP) 7 of Escherichia coli is a poorly characterized member of the family of enzymes that synthesize and modify the bacterial cell wall. The approximate chromosomal position of the gene encoding this protein was determined by measuring the expression of PBPs during lytic infection of E. col...

journal_title：Journal of bacteriology

authors： Henderson TA,Templin M,Young KD

更新日期：1995-04-01 00:00:00

abstract：:Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...

journal_title：Journal of bacteriology

authors： Huyer M,Page WJ

更新日期：1989-07-01 00:00:00

abstract：:Banerjea, A. (Rocky Mountain Laboratory, Hamilton, Mont.) and J. Munoz. Antigens of Bordetella pertussis. II, Purification of heat-labile toxin. J. Bacteriol. 84:269-274. 1962.-A mild method of separating heat-labile toxin of Bordetella pertussis from other cellular components is described; it consists of absorbing to...

journal_title：Journal of bacteriology

authors： BANERJEA A,MUNOZ J

更新日期：1962-08-01 00:00:00

abstract：:As part of an analysis of the conjugative transfer genes associated with the expression of F pili by plasmid F, we have investigated the physical location of the traC and traW genes. We found that plasmid clones carrying a 2.95-kilobase EcoRI-EcoRV F transfer operon fragment were able to complement transfer of F lac t...

journal_title：Journal of bacteriology

authors： Maneewannakul S,Kathir P,Moore D,Le LA,Wu JH,Ippen-Ihler K

更新日期：1987-11-01 00:00:00

abstract：:d-Desthiobiotin synthetase, an enzyme that catalyzes the synthesis of d-desthiobiotin from dl-7,8-diaminopelargonic acid and HCO(3) (-), was purified 100-fold from cells of a biotin mutant strain of Escherichia coli. Adenosine triphosphate and Mg(2+) were shown, especially in purified extracts, to be obligatory for en...

journal_title：Journal of bacteriology

authors： Cheeseman P,Pai CH

更新日期：1970-11-01 00:00:00

abstract：:In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we exa...

journal_title：Journal of bacteriology

authors： Chen YE,Tsokos CG,Biondi EG,Perchuk BS,Laub MT

更新日期：2009-12-01 00:00:00