Amino acid residues in the alpha-subunit C-terminal domain of Escherichia coli RNA polymerase involved in activation of transcription from the mtr promoter.

abstract：:The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased expression of the enzyme was realized by using a T7 expression system. The enzyme was purified and crystallized. A temperat...

journal_title：Journal of bacteriology

authors： Dallas WS,Gowen JE,Ray PH,Cox MJ,Dev IK

更新日期：1992-09-01 00:00:00

abstract：:The CO dehydrogenase structural genes (cox) and orf4 are clustered in the transcriptional order coxM--> coxS--> coxL--> orf4 on the 128-kb megaplasmid pHCG3 of the carboxidotroph Oligotropha carboxidovorans OM5. Sequence analysis suggested association of molybdopterin cytosine dinucleotide and flavin adenine dinucleot...

journal_title：Journal of bacteriology

authors： Schübel U,Kraut M,Mörsdorf G,Meyer O

更新日期：1995-04-01 00:00:00

abstract：:The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...

journal_title：Journal of bacteriology

authors： Kennell D,Kotoulas A

更新日期：1967-01-01 00:00:00

abstract：:The first enzymes of the histidine (hut) and proline degradative pathways, histidase and proline oxidase, could not be induced in Bacillus subtilis cells growing in glucose minimal medium containing a mixture of 16 amino acids. Addition of the 16-amino-acid mixture to induced wild-type cells growing in citrate minimal...

journal_title：Journal of bacteriology

authors： Atkinson MR,Wray LV Jr,Fisher SH

更新日期：1990-09-01 00:00:00

abstract：:Gas vesicles, isolated from lysed Halobacterium halobium cells, gave an amino acid analysis which accounted for 78% of the weight, and the balance was mainly salt and water. One percent of tightly bound d-galactose was found, as well as 2% of phosphate that was not released by treatment which promotes beta-elimination...

journal_title：Journal of bacteriology

authors： Krantz MJ,Ballou CE

更新日期：1973-06-01 00:00:00

abstract：:There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two ...

journal_title：Journal of bacteriology

authors： Gibson JL,Tabita FR

更新日期：1985-12-01 00:00:00

abstract：:Rabbits immunized with mucopeptide derived from cell wall debris of Streptococcus agalactiae by use of the formamide extraction technique developed specific antibodies for bovine heart, skeletal muscle, lymph nodes, and blood buffy coat extracts. ...

journal_title：Journal of bacteriology

authors： Dodd K,Norcross NL

更新日期：1967-02-01 00:00:00

abstract：:The degree to which deoxyribonucleic acid segregates nonrandomly has been investigated for Escherichia coli B/r growing in different media. The degree of nonrandom segregation observed is dependent on the medium, with segregation becoming less random as the growth rate decreases. This indicates that there must be some...

journal_title：Journal of bacteriology

authors： Cooper S,Weinberger M

更新日期：1977-04-01 00:00:00

abstract：:The rates of DNA synthesis during the cell-division cycle were measured in Myxococcus xanthus growing in three different media permitting a twofold variation in doubling time. In all three media, simple DNA cycles were observed. Synthesis of DNA occurred during 85% of the cell-division cycle, independent of generation...

journal_title：Journal of bacteriology

authors： Zusman DR,Krotoski DM,Cumsky M

更新日期：1978-01-01 00:00:00

abstract：:Transcription of the archaeal trkA gene homolog in Methanosarcina mazei S-6 was studied at the optimal growth temperature of 37 degrees C and after heat shock at 45 degrees C. Northern (RNA) blotting results (transcript size) and data from primer extension experiments to map the transcription initiation site indicate ...

journal_title：Journal of bacteriology

authors： Conway de Macario E,Macario AJ

更新日期：1995-11-01 00:00:00

abstract：:Bacterial pathogenesis depends on changes in metabolic and virulence gene expression in response to changes within a pathogen's environment. The plague-causing pathogen, Yersinia pestis, requires expression of the gene encoding the Pla protease for progression of pneumonic plague. The catabolite repressor protein Crp,...

journal_title：Journal of bacteriology

authors： Ritzert JT,Lathem WW

更新日期：2018-05-09 00:00:00

abstract：:The synthesis and export of aerolysin, an extracellular protein toxin released by the gram-negative bacterium Aeromonas hydrophila, was studied by pulse-labeling with [35S]methionine. The toxin was synthesized as a higher-molecular-weight precursor. This was processed cotranslationally, resulting in the appearance wit...

journal_title：Journal of bacteriology

authors： Howard SP,Buckley JT

更新日期：1985-03-01 00:00:00

abstract：:Two-dimensional gel analyses were made of proteins synthesized in Escherichia coli during various O2- -generating conditions. Nine proteins were constitutively synthesized over wild-type levels in superoxide dismutase (sodA sodB) double mutants. Addition of redox cycling agents such as paraquat and plumbagin at variou...

journal_title：Journal of bacteriology

authors： Walkup LK,Kogoma T

更新日期：1989-03-01 00:00:00

abstract：:SAL-TOL in vivo recombinant plasmid pKF439 was characterized in a strain from a mixed culture of bacteria harboring various degradative plasmids. Analysis of the gene organization of pKF439 revealed that the 57-kilobase TOL fragment, including the 40-kilobase TOL metabolic region, was inserted into the complete SAL re...

journal_title：Journal of bacteriology

authors： Furukawa K,Miyazaki T,Tomizuka N

更新日期：1985-06-01 00:00:00

abstract：:Strain IMCC3088, cultivated from the Yellow Sea, is a novel isolate belonging to the OM60/NOR5 clade and is closely related to clone OM241, Congregibacter litoralis, and strain HTCC2080. Here, the genome sequence of strain IMCC3088 is presented, showing the absence of photosynthetic gene clusters and the presence of p...

journal_title：Journal of bacteriology

authors： Jang Y,Oh HM,Kang I,Lee K,Yang SJ,Cho JC

更新日期：2011-07-01 00:00:00

abstract：:Mutational analysis of the carboxy-terminal region of Escherichia coli HlyC was performed by site-directed mutagenesis. Replacement of residue Val-127 or Lys-129 reduced the activity of HlyC to about 30 or 60%, respectively, of that of the wild type, while replacement of Gly-128 reduced the activity to less than 1% of...

journal_title：Journal of bacteriology

authors： Guzmán-Verri C,García F,Arvidson S

更新日期：1997-09-01 00:00:00

abstract：:The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all prepar...

journal_title：Journal of bacteriology

authors： Volk WA

更新日期：1968-03-01 00:00:00

abstract：:Isolated sheaths of Leptothrix discophora SP-6 (ATCC 51168) were tested for susceptibility to degradation by a variety of chemical denaturants and lytic enzymes and found to be resistant to many reagents and enzyme treatments. However, disulfide bond-reducing agents such as dithiothreitol (DTT), beta-mercaptoethanol, ...

journal_title：Journal of bacteriology

authors： Emerson D,Ghiorse WC

更新日期：1993-12-01 00:00:00

abstract：:In Listeria monocytogenes, the full details of how stress signals are integrated into the σB regulatory pathway are not yet available. To help shed light on this question, we investigated a collection of transposon mutants that were predicted to have compromised activity of the alternative sigma factor B (σB). These m...

journal_title：Journal of bacteriology

authors： Guerreiro DN,Wu J,Dessaux C,Oliveira AH,Tiensuu T,Gudynaite D,Marinho CM,Boyd A,García-Del Portillo F,Johansson J,O'Byrne CP

更新日期：2020-04-09 00:00:00

abstract：:Antibiotic resistance in Neisseria gonorrhoeae has been associated with the acquisition of R plasmids from heterologous organisms. The broad-host-range plasmids of incompatibility groups P (IncP) and Q (IncQ) have played a role in this genetic exchange in nature. We have utilized derivatives of RSF1010 (IncQ) and RP1 ...

journal_title：Journal of bacteriology

authors： Butler CA,Gotschlich EC

更新日期：1991-09-01 00:00:00

abstract：:After UV irradiation, Escherichia coli uvrA mutant cells show higher survival on minimal than on rich growth medium, i.e., they show minimal-medium recovery. This effect of rich growth medium on survival is not observed in a uvrA mutant carrying an mmrA1 mutation, and the uvrA mmrA strain showed the same survival rate...

journal_title：Journal of bacteriology

authors： Sharma RC,Sargentini NJ,Smith KC

更新日期：1983-05-01 00:00:00

abstract：:Induction of colicin la at a high temperature (42 C) was demonstrated in a wild strain of Shigella sonnei. After transfer of the Col factor, a similar effect was observed in those recipient cells acquiring colicinogeny. The induction is suggested to be due to the presence of a thermosensitive colicin repressor. The wi...

journal_title：Journal of bacteriology

authors： Gromkova RH

更新日期：1971-06-01 00:00:00

abstract：:Although four of the five Salmonella pathogenicity islands (SPIs) have been characterized in detail for Salmonella enterica serovar Typhimurium, and the fifth has been characterized for Salmonella enterica serovar Dublin, there have been limited studies to examine them in detail in a range of pathogenic serovars of S....

journal_title：Journal of bacteriology

authors： Amavisit P,Lightfoot D,Browning GF,Markham PF

更新日期：2003-06-01 00:00:00

abstract：:A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...

journal_title：Journal of bacteriology

authors： Butterton JR,Choi MH,Watnick PI,Carroll PA,Calderwood SB

更新日期：2000-03-01 00:00:00

abstract：:Tyrosine-mediated repression of aroF and tyrP was studied by inserting DNA sequences between the two adjacent TYR R boxes which, in each case, overlap the respective RNA polymerase binding sites of these genes. In both cases, repression was greatest when homologous regions of these two TYR R boxes were on the same fac...

journal_title：Journal of bacteriology

authors： Andrews AE,Dickson B,Lawley B,Cobbett C,Pittard AJ

更新日期：1991-08-01 00:00:00

abstract：:Degradation of 2-hydroxy-5-carboxymethylmuconic semialdehyde, the ring fission product of the 4-hydroxyphenylacetate meta-cleavage pathway, by mutant strains P23X19 and P23X16 of Pseudomonas putida NCI B 9865 was studied. Both mutants were unable to grow on either 4-hydroxyphenylacetate of 3,4-dihydroxyphenylacetate. ...

journal_title：Journal of bacteriology

authors： Barbour MG,Bayly RC

更新日期：1980-05-01 00:00:00

abstract：:A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...

journal_title：Journal of bacteriology

authors： Taylor BF,Campbell WL,Chinoy I

更新日期：1970-05-01 00:00:00

abstract：:Two proteins that bind penicillin were observed in Escherichia coli infected with lambda phages 141, 142, 650, and 651 from the Kohara genomic library. These phages carry chromosomal DNA fragments that do not contain any known penicillin binding protein (PBP) genes, indicating that unrecognized gene products were exhi...

journal_title：Journal of bacteriology

authors： Henderson TA,Young KD,Denome SA,Elf PK

更新日期：1997-10-01 00:00:00

abstract：:Fine details of the internal and external morphology of Cryptococcus neoformans as seen in ultrathin sections are described and illustrated with electron micrographs. The capsule characteristic of this species contained microfibrils (30 to 40 A in diameter) that appeared to radiate from the cell wall and to coil and i...

journal_title：Journal of bacteriology

authors： Edwards MR,Gordon MA,Lapa EW,Ghiorse WC

更新日期：1967-09-01 00:00:00

abstract：:The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...

journal_title：Journal of bacteriology

authors： Chou CC,Lin TW,Chen CY,Wang AH

更新日期：2003-07-01 00:00:00