Abstract:
:Fine details of the internal and external morphology of Cryptococcus neoformans as seen in ultrathin sections are described and illustrated with electron micrographs. The capsule characteristic of this species contained microfibrils (30 to 40 A in diameter) that appeared to radiate from the cell wall and to coil and intertwine in various directions. These thin, uniformly structured, electron-dense filaments are believed to represent complex polysaccharide molecules. The internal morphology of C. neoformans was in many ways similar to that of yeasts studied by other authors. The cell was uninucleate with a single nucleolus. The nuclear envelope, a pair of unit membranes interrupted by pores, was typical of that found in eucaryotic organisms. Smooth endoplasmic reticulum, mitochondria, vacuoles, storage granules, and ribosomes were consistent features of the cytoplasm. In addition, C. neoformans presented membranous organelles derived from the plasma membrane and comparable to bacterial mesosomes and mitochondria of an annulate type.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Edwards MR,Gordon MA,Lapa EW,Ghiorse WCdoi
10.1128/JB.94.3.766-777.1967subject
Has Abstractpub_date
1967-09-01 00:00:00pages
766-77issue
3eissn
0021-9193issn
1098-5530journal_volume
94pub_type
杂志文章abstract::One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase-like open reading frames (ORFs) were found besides one ORF having the typical alpha-like DNA poly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.8.2232-2236.1998
更新日期:1998-04-01 00:00:00
abstract::In Escherichia coli, cell division is mediated by the concerted action of about 12 proteins that assemble at the division site to presumably form a complex called the divisome. Among these essential division proteins, the multimodular class B penicillin-binding protein 3 (PBP3), which is specifically involved in septa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.18.6110-6117.2004
更新日期:2004-09-01 00:00:00
abstract::The polymerase chain reaction (PCR) has been used to amplify DNA fragments by using eucaryotic genomic DNA as a template. We show that bacterial genomic DNA can be used as a template for PCR amplification. We demonstrate that DNA fragments at least as large as 4,400 base pairs can be amplified with fidelity and that t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1602-1608.1989
更新日期:1989-03-01 00:00:00
abstract::The N-terminal sequence of a protein, originally described as an adhesin of Helicobacter pylori, was used in an oligonucleotide-based screening procedure of an H. pylori plasmid library in Escherichia coli. Five independent plasmid clones were isolated, all mapping to the same chromosomal region and encoding the H. py...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.6960-6967.1996
更新日期:1996-12-01 00:00:00
abstract::Two chemotactic transducers for inorganic phosphate (P(i)), designated CtpH and CtpL, have been identified in Pseudomonas aeruginosa. The corresponding genes (ctpH and ctpL) were inactivated by inserting kanamycin and tetracycline resistance gene cassettes into the wild-type genes in the P. aeruginosa PAO1 genome. Com...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.12.3400-3404.2000
更新日期:2000-06-01 00:00:00
abstract::Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.6.1624-1626.1995
更新日期:1995-03-01 00:00:00
abstract::Altenbern, Robert A. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.). Growth of L forms of streptomycin-dependent Proteus mirabilis. J. Bacteriol. 85:619-622. 1963.-A streptomycin-dependent mutant of Proteus mirabilis produces L forms on penicillin-containing medium when dihydrostreptomycin is also p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.3.619-622.1963
更新日期:1963-03-01 00:00:00
abstract::The oral bacterium Streptococcus salivarius takes up lactose via a transporter called LacS that shares 95% identity with the LacS from Streptococcus thermophilus, a phylogenetically closely related organism. S. thermophilus releases galactose into the medium during growth on lactose. Expulsion of galactose is mediated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.23.6764-6772.2003
更新日期:2003-12-01 00:00:00
abstract::Calcium carbonate precipitation, a widespread phenomenon among bacteria, has been investigated due to its wide range of scientific and technological implications. Nevertheless, little is known of the molecular mechanisms by which bacteria foster calcium carbonate mineralization. In our laboratory, we are studying calc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01450-06
更新日期:2007-01-01 00:00:00
abstract::The genome sequences of several pseudomonads have revealed a gene cluster containing genes for a two-component heavy metal histidine sensor kinase and response regulator upstream of cinA and cinQ, which we show herein to encode a copper-containing azurin-like protein and a pre-Q(0) reductase, respectively. In the pres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00377-07
更新日期:2007-07-01 00:00:00
abstract::The morphology of nucleoids and mesosomes of Bacillus subtilis in stationary-and lag-phase cultures was studied by making three-dimensional cell reconstructions in plastic of electron micrographs of serial sections. In cells from stationary cultures, the dormant nucleoids are frequently, but not always, spherical and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.3.1189-1199.1975
更新日期:1975-03-01 00:00:00
abstract::The gene (entD) encoding staphylococcal enterotoxin D (SED) has been located on a 27.6-kilobase penicillinase plasmid designated pIB485. This plasmid was present in all SED-producing strains tested. The entD gene was cloned on a 2.0-kilobase DNA fragment and was expressed in Escherichia coli. Sequence analysis of this...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4799-4806.1989
更新日期:1989-09-01 00:00:00
abstract::A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.35-45.1996
更新日期:1996-01-01 00:00:00
abstract::Among 120 strains of gliding bacteria which were screened for restriction endonucleases, 27 were found positive. Additionally, three strains carried enzymes able to release the supercoiled state of closed circular DNA. By using a new rapid method, restriction endonuclease activity was released by stirring about 0.5 g ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.708-713.1978
更新日期:1978-11-01 00:00:00
abstract::The tsr gene specifies a methyl-accepting membrane protein involved in chemotaxis to serine and several repellent compounds. We have characterized a special class of tsr mutations designated cheD which alter the signaling properties of the Tsr transducer. Unlike tsr null mutants, cheD strains are generally nonchemotac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.96-104.1985
更新日期:1985-01-01 00:00:00
abstract::The accumulation of imidazoleglycerol phosphate during growth of Neurospora crassa in the presence of 3-amino-1,2,4-triazole was found to cause derepression of tryptophan synthetase and to inhibit the induction of kynureninase. Accumulation of indoleglycerol phosphate in response to growth in the presence of indole ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.5.1608-1614.1968
更新日期:1968-05-01 00:00:00
abstract::The surface structure and cell envelope layers of various virulent Bacteroides nodosus strains were examined by light microscopy and by electron microscopy by using negative staining, thin-section, and freeze-fracture-etch techniques. Three surface structures were described: pili and a diffuse material, both of which ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.845-857.1980
更新日期:1980-02-01 00:00:00
abstract::The ribosome content was markedly reduced when illumination (at 28 C) was repeatedly interrupted; an opposite trend was observed with continuous light as the temperature was decreased. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1518-1519.1969
更新日期:1969-03-01 00:00:00
abstract::Although Salmonella typhimurium prefers neutral-pH environments, it can adapt to survive conditions of severe low-pH stress (pH 3.3). The process, termed the acid tolerance response (ATR), includes two distinct stages. The first stage, called pre-acid shock, is induced at pH 5.8 and involves the production of an induc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.7.1981-1987.1993
更新日期:1993-04-01 00:00:00
abstract::Lignin peroxidases (LiP) of Phanerochaete chrysosporium are encoded by a family of six closely related genes. Five LiP genes have been localized to the same dimorphic chromosome. In this investigation, relative transcript levels of the LiP genes were determined. Transcripts of the LiPA, LiPB, and O282 genes were at si...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.5036-5042.1992
更新日期:1992-08-01 00:00:00
abstract::Carbon catabolite repression (CCR) allows bacteria to alter metabolism in response to the availability of specific sugar sources, and increasing evidence suggests that CCR is involved in regulating virulence gene expression in many pathogens. A scan of the M1 SF370 group A streptococcus (GAS) genome using a Bacillus s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01038-07
更新日期:2007-12-01 00:00:00
abstract::The terminal step of triacylglycerol (TAG) formation in the yeast Saccharomyces cerevisiae is catalyzed by the enzyme acyl-CoA:diacylglycerol acyltransferase (DAGAT). In this study we demonstrate that the gene product of YOR245c, Dga1p, catalyzes a major yeast DAGAT activity which is localized to lipid particles. Enzy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.2.519-524.2002
更新日期:2002-01-01 00:00:00
abstract::Genetic and segregation analysis of Escherichia coli strains containing a partial duplication of the trp operon reveal that the 2.5-min-long region trpD-purB is duplicated in tandem in the chromosome. The adjacent loci cysB and fabD are not duplicated. Although one copy of the duplicated region is longer than the maxi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.650-660.1978
更新日期:1978-02-01 00:00:00
abstract::Strains of Escherichia coli K12 have been prepared that carry the ruv mutation in combination with lon or uvrA mutant alleles. The ruv minus uvrA minus double mutant is more sensitive to ultraviolet light than the urvA minus single mutant, whereas the strain with ruv and ion mutations shows the same sensitivity to ult...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.735-736.1975
更新日期:1975-02-01 00:00:00
abstract::mRNA levels from the bop (bacterio-opsin), brp (bacterio-opsin-related protein), and bat (bacterio-opsin activator) genes in wild-type Halobacterium halobium and two bacteriorhodopsin-overproducing mutants (ET1001 and II-7) were quantitated under conditions in which oxygen levels were steadily depleted and then cultur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.6.1655-1660.1994
更新日期:1994-03-01 00:00:00
abstract::Cells of Clostridium thermosaccharolyticum grown under strict anaerobiosis (modified Hungate technique) were examined during growth and sporulation by employing Nomarski interference-contrast and Zernike phase-contrast optics to delineate the sequence of morphological changes leading to the formation of free, mature s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.3.674-681.1972
更新日期:1972-09-01 00:00:00
abstract::Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin, transferrin, ferritin, and ferric citrate as iron sources. Whole-genome DNA microarrays were used to monitor global gene expression over seven time points after the addition of the defined iron source to the medium. Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.23.6714-6720.2002
更新日期:2002-12-01 00:00:00
abstract::Wiley, W. R. (Washington State University, Pullman) and J. L. Stokes. Requirement of an alkaline pH and ammonia for substrate oxidation by Bacillus pasteurii. J. Bacteriol. 84:730-734. 1962-Resting-cell suspensions of Bacillus paseurii require a pH higher than 8.0 and also NH(4) (+) for the oxidation of glycine, alani...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.4.730-734.1962
更新日期:1962-10-01 00:00:00
abstract::At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.2.658-667.1970
更新日期:1970-11-01 00:00:00
abstract::Isolation of six strains of Escherichia coli K-12 carrying mutations affecting the citrate-dependent iron uptake system is described. Genetic analysis of these mutants showed that the mutation affecting the citrate system are cluster together at about min 6 on the E. coli chromosome. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.3.1524-1526.1978
更新日期:1978-03-01 00:00:00