Abstract:
:Wiley, W. R. (Washington State University, Pullman) and J. L. Stokes. Requirement of an alkaline pH and ammonia for substrate oxidation by Bacillus pasteurii. J. Bacteriol. 84:730-734. 1962-Resting-cell suspensions of Bacillus paseurii require a pH higher than 8.0 and also NH(4) (+) for the oxidation of glycine, alanine, serine, glutamic acid, other amino acids, and compounds of the Krebs cycle. The optimal pH is 9.2. Maximal activity was obtained with 0.5% (NH(4))(2)SO(4), the highest concentration tested. NH(4) (+) can be supplied by a number of inorganic and organic ammonium salts. The effect of NH(4) (+) is fairly specific. It cannot be replaced by Na(+), Cs(+), and Mg(++), and only partially replaced by Li(+), K(+), Rb(+), and Ca(++). Some possible metabolic functions of the required alkaline pH and NH(4) (+) are discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
WILEY WR,STOKES JLdoi
10.1128/JB.84.4.730-734.1962keywords:
subject
Has Abstractpub_date
1962-10-01 00:00:00pages
730-4eissn
0021-9193issn
1098-5530journal_volume
84pub_type
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