Requirement of an alkaline pH and ammonia for substrate oxidation by Bacillus pasteurii.

Abstract:

:Wiley, W. R. (Washington State University, Pullman) and J. L. Stokes. Requirement of an alkaline pH and ammonia for substrate oxidation by Bacillus pasteurii. J. Bacteriol. 84:730-734. 1962-Resting-cell suspensions of Bacillus paseurii require a pH higher than 8.0 and also NH(4) (+) for the oxidation of glycine, alanine, serine, glutamic acid, other amino acids, and compounds of the Krebs cycle. The optimal pH is 9.2. Maximal activity was obtained with 0.5% (NH(4))(2)SO(4), the highest concentration tested. NH(4) (+) can be supplied by a number of inorganic and organic ammonium salts. The effect of NH(4) (+) is fairly specific. It cannot be replaced by Na(+), Cs(+), and Mg(++), and only partially replaced by Li(+), K(+), Rb(+), and Ca(++). Some possible metabolic functions of the required alkaline pH and NH(4) (+) are discussed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

WILEY WR,STOKES JL

doi

10.1128/JB.84.4.730-734.1962

keywords:

subject

Has Abstract

pub_date

1962-10-01 00:00:00

pages

730-4

eissn

0021-9193

issn

1098-5530

journal_volume

84

pub_type

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