Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins.

abstract：:Vibrio vulnificus is an estuarine bacterium and potent opportunistic human pathogen. It enters the food chain by asymptomatically colonizing a variety of marine organisms, most notably oysters. Expression of the brp-encoded extracellular polysaccharide, which enhances cell-surface adherence, is regulated by cyclic di-...

journal_title：Journal of bacteriology

authors： Chodur DM,Guo L,Pu M,Bruger E,Fernandez N,Waters C,Rowe-Magnus DA

更新日期：2017-09-05 00:00:00

abstract：:The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehy...

journal_title：Journal of bacteriology

authors： Beck C,von Meyenburg HK

更新日期：1968-08-01 00:00:00

abstract：:Heterocyst development was analyzed in mutants of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 bearing inactivated cox2 and/or cox3 genes, encoding heterocyst-specific terminal respiratory oxidases. At the morphological level, the cox2 cox3 double mutant (strain CSAV141) was impaired in membrane ...

journal_title：Journal of bacteriology

authors： Valladares A,Maldener I,Muro-Pastor AM,Flores E,Herrero A

更新日期：2007-06-01 00:00:00

abstract：:Manganese is accumulated in Bacillus subtilis by a highly specific active transport system. This trace element "pump" is insensitive to added magnesium or calcium and preferentially accumulates manganese in the presence of cobalt, iron, and copper. Manganese uptake in B. subtilis is inhibited by cyanide, azide, pentac...

journal_title：Journal of bacteriology

authors： Eisenstadt E,Fisher S,Der CL,Silver S

更新日期：1973-03-01 00:00:00

abstract：:The nucleotide sequence of the pathogenic spirochete Brachyspira hyodysenteriae bit (for "Brachyspira iron transport") genomic region has been determined. The bit region is likely to encode an iron ATP-binding cassette transport system with some homology to those encountered in gram-negative bacteria. Six open reading...

journal_title：Journal of bacteriology

authors： Dugourd D,Martin C,Rioux CR,Jacques M,Harel J

更新日期：1999-11-01 00:00:00

abstract：:Tryptophan is an essential amino acid that is required for normal development in Chlamydia species, and tryptophan metabolism has been implicated in chlamydial persistence and tissue tropism. The ability to synthesize tryptophan is not universal among the Chlamydiaceae, but species that have a predicted tryptophan bio...

journal_title：Journal of bacteriology

authors： Akers JC,Tan M

更新日期：2006-06-01 00:00:00

abstract：:Amino acid transport rates and amino acid binding proteins were examined in a strain containing the rho-120 mutation (formerly SuA), which has been shown to lower the rho-dependent, ribonucleic acid-activated adenosine triphosphatase activity to 9% of the rho activity in the isogenic wild-type strain. Tryptophan and p...

journal_title：Journal of bacteriology

authors： Quay SC,Oxender DL

更新日期：1977-06-01 00:00:00

abstract：:Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...

journal_title：Journal of bacteriology

authors： Golub EI,Low KB

更新日期：1985-04-01 00:00:00

abstract：:The function of serine protease EpiP in epidermin biosynthesis was investigated. Epidermin is synthesized as a 52-amino-acid precursor peptide, EpiA, which is posttranslationally modified and processed to the mature 22-amino-acid peptide antibiotic. epiP was expressed in Staphylococcus carnosus with xylose-regulated e...

journal_title：Journal of bacteriology

authors： Geissler S,Götz F,Kupke T

更新日期：1996-01-01 00:00:00

abstract：:Pediococcus halophilus X-160 which lacks catabolite control by glucose was isolated from nature (soy moromi mash). Wild-type strains, in xylose-glucose medium, utilized glucose preferentially over xylose and showed diauxic growth. With wild-type strain I-13, xylose isomerase activity was not induced until glucose was ...

journal_title：Journal of bacteriology

authors： Abe K,Uchida K

更新日期：1989-04-01 00:00:00

abstract：:Cyclic adenosine 3',5'-monophosphate isolated from Mycobacterium smegmatis cells was identified by thin-layer chromatography, stepwise conversion to adenosine 5'-monophosphate and adenosine, ultraviolet absorption spectrum, phosphate analysis, and detection by two relatively specific radioisotopic methods. ...

journal_title：Journal of bacteriology

authors： Lee CH

更新日期：1977-12-01 00:00:00

abstract：:Streptococcus agalactiae (group B streptococcus [GBS]) is a major cause of infections in newborns, pregnant women, and immunocompromised patients. GBS strain CNCTC10/84 is a clinical isolate that has high virulence in animal models of infection and has been used extensively to study GBS pathogenesis. Two unusual featu...

journal_title：Journal of bacteriology

authors： Zhu L,Beres SB,Yerramilli P,Pruitt L,Cantu CC,Olsen RJ,Musser JM

更新日期：2020-11-04 00:00:00

abstract：:Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...

journal_title：Journal of bacteriology

authors： Rushing BG,Long SR

更新日期：1995-12-01 00:00:00

abstract：:Streptococcus cremoris cells that had been grown in a chemostat were starved for lactose. The viability of the culture remained essentially constant in the first hours of starvation and subsequently declined logarithmically. The viability pattern during starvation varied with the previously imposed growth rates. The d...

journal_title：Journal of bacteriology

authors： Poolman B,Smid EJ,Veldkamp H,Konings WN

更新日期：1987-04-01 00:00:00

abstract：:During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...

journal_title：Journal of bacteriology

authors： Johnsen U,Schönheit P

更新日期：2004-09-01 00:00:00

abstract：:Two novel insertion sequences, IS1312 and IS1313, were found in pTiBo542, the Ti plasmid of Agrobacterium tumefaciens strains Bo542 and A281. Nucleotide sequencing and Southern hybridization revealed that IS1312 and IS1313 are homologous to Rhizobium meliloti ISRm1 and ISRm2, respectively. IS1312, ISRm1, and another A...

journal_title：Journal of bacteriology

authors： Deng W,Gordon MP,Nester EW

更新日期：1995-05-01 00:00:00

abstract：:Antibiotic resistance in Neisseria gonorrhoeae has been associated with the acquisition of R plasmids from heterologous organisms. The broad-host-range plasmids of incompatibility groups P (IncP) and Q (IncQ) have played a role in this genetic exchange in nature. We have utilized derivatives of RSF1010 (IncQ) and RP1 ...

journal_title：Journal of bacteriology

authors： Butler CA,Gotschlich EC

更新日期：1991-09-01 00:00:00

abstract：:The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 pr...

journal_title：Journal of bacteriology

authors： Mercer AA,Loutit JS

更新日期：1979-10-01 00:00:00

abstract：:A set of Saccharomyces cerevisiae strains with variable expression of only the high-affinity Hxt7 glucose transporter was constructed by partial deletion of the HXT7 promoter in vitro and integration of the gene at various copy numbers into the genome of an hxt1-7 gal2 deletion strain. The glucose transport capacity i...

journal_title：Journal of bacteriology

authors： Ye L,Kruckeberg AL,Berden JA,van Dam K

更新日期：1999-08-01 00:00:00

abstract：:A gene specifying the enzyme nicotinamide deamidase has been mapped, by the interrupted mating technique, at about 33 min on the chromosome of Escherichia coli K-12. ...

journal_title：Journal of bacteriology

authors： Dickinson ES,Sundaram TK

更新日期：1970-03-01 00:00:00

abstract：:Six analogues of amino acids killed corresponding auxotrophs of Escherichia coli. With all but one analogue, protein synthesis was required for lethality. ...

journal_title：Journal of bacteriology

authors： Rabinovitz M,Finkleman A,Reagan RL,Breitman TR

更新日期：1969-07-01 00:00:00

abstract：:Grown in liquid culture in the presence of a variety of structurally unrelated drugs, mycelia of wild-type Neurospora assume a colonial or semicolonial growth habit similar to that of known morphological mutants. Drugs that produce these morphological changes include atropine, theophylline, histamine, and several of t...

journal_title：Journal of bacteriology

authors： Scott WA,Solomon B

更新日期：1975-05-01 00:00:00

abstract：:Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...

journal_title：Journal of bacteriology

authors： Peleg A,Shifrin Y,Ilan O,Nadler-Yona C,Nov S,Koby S,Baruch K,Altuvia S,Elgrably-Weiss M,Abe CM,Knutton S,Saper MA,Rosenshine I

更新日期：2005-08-01 00:00:00

abstract：:Hottle, G. A. (Naval Biological Laboratory, University of California, Berkeley), and D. N. Wright. Growth and survival of Mycoplasma neurolyticum in liquid media. J. Bacteriol. 91:1834-1839. 1966.-Maximal growth of Mycoplasma neurolyticum (between 10(8) and 10(9) colony-forming units per ml) was obtained after 3 days ...

journal_title：Journal of bacteriology

authors： Hottle GA,Wright DN

更新日期：1966-05-01 00:00:00

abstract：:Growth of Salmonella typhimurium on acetate as a sole carbon source requires expression of the glyoxylate shunt; however, the genes for the glyoxylate shunt enzymes have not been previously identified in S. typhimurium. In this study, we isolated transposon insertions in the genes for the two unique enzymes of this pa...

journal_title：Journal of bacteriology

authors： Wilson RB,Maloy SR

更新日期：1987-07-01 00:00:00

abstract：:XbaI digestion and pulsed-field gel electrophoresis of the genome of Salmonella typhimurium LT2 yields 24 fragments: 23 fragments (total size, 4,807 kb) are from the chromosome, and one fragment (90 kb) is from the virulence plasmid pSLT. Some of the 23 fragments from the chromosome were located on the linkage map by ...

journal_title：Journal of bacteriology

authors： Liu SL,Sanderson KE

更新日期：1992-03-01 00:00:00

abstract：:Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activiti...

journal_title：Journal of bacteriology

authors： Habe H,Chung JS,Kato H,Ayabe Y,Kasuga K,Yoshida T,Nojiri H,Yamane H,Omori T

更新日期：2004-09-01 00:00:00

abstract：:The response of Salmonella typhimurium to various forms of environmental stress was examined by using O'Farrell two-dimensional gel electrophoresis. Polypeptides (a total of 110) which quantitatively increased during various starvations, anaerobiosis, or heat shock were identified and cataloged in reference to a stand...

journal_title：Journal of bacteriology

authors： Spector MP,Aliabadi Z,Gonzalez T,Foster JW

更新日期：1986-10-01 00:00:00

abstract：:Four chloramphenicol resistance (Cm) and four tetracycline resistance (Tc) plasmids from Staphylococcus aureus were characterized by restriction endonuclease mapping. All four Tc plasmids had molecular masses of 2.9 megadaltons (Mdaltons) and indistinguishable responses to seven different restriction endonucleases. Th...

journal_title：Journal of bacteriology

authors： Wilson CR,Baldwin NJ

更新日期：1978-10-01 00:00:00

abstract：:The strict human pathogen Neisseria gonorrhoeae is exposed to oxidative damage during infection. N. gonorrhoeae has many defenses that have been demonstrated to counteract oxidative damage. However, recN is the only DNA repair and recombination gene upregulated in response to hydrogen peroxide (H(2)O(2)) by microarray...

journal_title：Journal of bacteriology

authors： Stohl EA,Seifert HS

更新日期：2006-11-01 00:00:00