Abstract:
:The nucleotide sequence of the pathogenic spirochete Brachyspira hyodysenteriae bit (for "Brachyspira iron transport") genomic region has been determined. The bit region is likely to encode an iron ATP-binding cassette transport system with some homology to those encountered in gram-negative bacteria. Six open reading frames oriented in the same direction and physically linked have been identified. This system possesses a protein containing ATP-binding motifs (BitD), two hydrophobic cytoplasmic membrane permeases (BitE and BitF), and at least three lipoproteins (BitA, BitB, and BitC) with homology to iron periplasmic binding proteins. These periplasmic binding proteins exhibit lipoprotein features. They are labeled by [(3)H]palmitate when tested in recombinant Escherichia coli, and their signal peptides are typical for substrates of the type II secretory peptidase. The FURTA system and Congo red assay indicate that BitB and BitC are involved in iron binding. The Bit system is detected only in B. hyodysenteriae and is absent from B. innocens and B. pilosicoli.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Dugourd D,Martin C,Rioux CR,Jacques M,Harel Jdoi
10.1128/JB.181.22.6948-6957.1999keywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
6948-57issue
22eissn
0021-9193issn
1098-5530journal_volume
181pub_type
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
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更新日期:1986-10-01 00:00:00
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更新日期:1991-09-01 00:00:00
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更新日期:1989-05-01 00:00:00
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更新日期:2019-08-08 00:00:00