Abstract:
:Growth of Salmonella typhimurium on acetate as a sole carbon source requires expression of the glyoxylate shunt; however, the genes for the glyoxylate shunt enzymes have not been previously identified in S. typhimurium. In this study, we isolated transposon insertions in the genes for the two unique enzymes of this pathway, aceA (isocitrate lyase) and aceB (malate synthase). The aceA and aceB genes were located at 89.5 min on the S. typhimurium genetic map. Genetic linkage to nearby loci indicated that the relative gene order is purDJ metA aceB aceA. Transposon insertions in aceB were polar on aceA, suggesting that the genes form an operon transcribed from aceB to aceA. Transcriptional regulation of the aceBA operon was studied by constructing mini-Mu d(lac Kan) operon fusions. Analysis of these fusions indicated that expression of the aceBA operon is regulated at the level of transcription; the aceBA genes were induced when acetate was present and repressing carbon sources were absent. Although glucose represses expression of the aceBA operon, repression does not seem to be mediated solely by cyclic AMP-cyclic AMP receptor protein complex. Mutants with altered regulation of the aceBA operon were isolated.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Wilson RB,Maloy SRdoi
10.1128/jb.169.7.3029-3034.1987subject
Has Abstractpub_date
1987-07-01 00:00:00pages
3029-34issue
7eissn
0021-9193issn
1098-5530journal_volume
169pub_type
杂志文章abstract::The genes encoding Shiga toxin (stx), the major virulence factor of Shiga toxin-encoding Escherichia coli (STEC) strains, are carried on lambdoid prophages resident in all known STEC strains. The stx genes are expressed only during lytic growth of these temperate bacteriophages. We cloned the gene encoding the repress...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7659-7669.2004
更新日期:2004-11-01 00:00:00
abstract::The obligate psychrophilic yeasts Torulopsis psychrophila, T. austromarina, Leucosporidium frigidum, L. gelidum, and L. nivalis were obligate aerobes and were unable to grow anaerobically. In contrast, the obligate thermophilic yeasts T. bovina, T. pintolopesii, Candida slooffii, and Saccharomyces telluris were facult...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.815-817.1978
更新日期:1978-11-01 00:00:00
abstract::A group of enzymes of sulfur metabolism (arylsulfatase, cholinesulfatase, and a number of others) are normally repressed in Neurospora crassa by an abundant supply of a "favored" sulfur source such as methionine or inorganic sulfate. A mutant called scon(c) was isolated in which the formation of each of these enzymes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.1.140-151.1972
更新日期:1972-01-01 00:00:00
abstract::Microbial communities span many orders of magnitude, ranging in scale from hundreds of cells on a single particle of soil to billions of cells within the lumen of the gastrointestinal tract. Bacterial cells in all habitats are members of densely populated local environments that facilitate competition between neighbor...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00275-16
更新日期:2016-07-28 00:00:00
abstract::Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2318-2321.1987
更新日期:1987-05-01 00:00:00
abstract::Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.1119-1136.1971
更新日期:1971-03-01 00:00:00
abstract::A strain of Bacillus subtilis, UVSSP-42-1, which produces ultraviolet (UV)-sensitive spores and vegetative cells, was found to possess germinated spores 25 times more UV resistant than the resting spores. This relative resistance achieved upon germination was associated with the transition of the heat-resistant refrac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.59-65.1974
更新日期:1974-10-01 00:00:00
abstract::The cobB function of Salmonella typhimurium LT2 was defined in vivo as an alternative activity for the nicotinic acid mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase enzyme (CobT), which is involved in the assembly of the nucleotide loop of cobalamin in this bacterium (J. R. Trzebiatowski, G. A. O'T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.7016-7019.1996
更新日期:1996-12-01 00:00:00
abstract::Mutant strains of Escherichia coli K-12 have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (trp) is partially constitutive. The mutation causing derepression is closely linked to aroH [the structural gene for DAHP synthetase (trp)] and occurs in a locus design...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.2.406-414.1971
更新日期:1971-08-01 00:00:00
abstract::No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.4.1425-1430.1968
更新日期:1968-04-01 00:00:00
abstract::To investigate the involvement of bacterial catalases of the symbiotic gram-negative bacterium Rhizobium meliloti in the development of Medicago-Rhizobium functional nodules, we cloned a putative kat gene by screening a cosmid library with a catalase-specific DNA probe amplified by PCR from the R. meliloti genome. Nuc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.6802-6809.1996
更新日期:1996-12-01 00:00:00
abstract::A temperature-sensitive mutant of Escherichia coli with a temperature-dependent change in the control of initiation of DNA replication was isolated. The phenotype of the mutant was dependent on a mutation in the RNA polymerase gene rpoC. In vitro RNA polymerase activity was temperature sensitive. The mutant grew and s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.443-451.1983
更新日期:1983-04-01 00:00:00
abstract::The thermosensitive growth phenotype exerted by runaway-mutant plasmids was suppressed by sublethal doses of the DNA gyrase inhibitors novobiocin or nalidixic acid, although the latter drug was less efficient. A novobiocin-resistant gyrB mutant Escherichia coli strain prevented expression of the runaway phenotype at 3...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.855-857.1985
更新日期:1985-05-01 00:00:00
abstract::Escherichia coli O111a is a thermosensitive strain which, when grown at 40 C, accumulates large quantities of intracellular membranes. The ultrastructure of these membranes in cells which have been chemically fixed, embedded, and examined as thin sections has been compared with that of membranes in cells negatively st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.433-444.1973
更新日期:1973-01-01 00:00:00
abstract::Mutants of the fhuA gene of Escherichia coli K-12, which encodes a receptor protein in the outer membrane, took up ferrichrome after exposure to pronase, whereas fhuB mutants remained transport negative. The latter finding supports our previous proposal that fhuB mutants are defective in a function that residues in th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.3.1158-1161.1981
更新日期:1981-06-01 00:00:00
abstract::The hook-basal body (HBB) is a key intermediate structure in the flagellar assembly pathway in Salmonella typhimurium. The FlgM protein inhibits the flagellum-specific transcription factor sigma28 in the absence of the intact HBB structure and is secreted out of the cell following HBB completion. The flk gene encodes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.20.5384-5397.1998
更新日期:1998-10-01 00:00:00
abstract::Commonly used procedures effected the extraction of only 10% of the lipoteichoic acid of stationary-phase cells of Staphylococcus aureus and Streptococcus faecium, unless the cells were first disrupted. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.399-402.1982
更新日期:1982-01-01 00:00:00
abstract::The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.5.1364-1373.2000
更新日期:2000-03-01 00:00:00
abstract::Candida albicans produces large amounts of the acyclic pentitol D-arabitol in culture and in infected animals and humans, and most strains also grow on minimal D-arabitol medium. An earlier study showed that the major metabolic precursor of D-arabitol in C. albicans was D-ribulose-5-PO4 from the pentose pathway, that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.11.2971-2976.1995
更新日期:1995-06-01 00:00:00
abstract::The MurNAc etherase MurQ of Escherichia coli is essential for the catabolism of the bacterial cell wall sugar N-acetylmuramic acid (MurNAc) obtained either from the environment or from the endogenous cell wall (i.e., recycling). High-level expression of murQ is required for growth on MurNAc as the sole source of carbo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00642-08
更新日期:2008-10-01 00:00:00
abstract::We report the draft genome sequence of methicillin-resistant Staphylococcus aureus (MRSA) strain ST672, an emerging disease clone in India, from a septicemia patient. The genome size is about 2.82 Mb with 2,485 open reading frames (ORFs). The staphylococcal cassette chromosome mec (SCCmec) element (type V) and immune ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01868-12
更新日期:2012-12-01 00:00:00
abstract::The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2165-2170.1968
更新日期:1968-06-01 00:00:00
abstract::Eubacterium sp. strain VPI 12708 is an anaerobic intestinal bacterium that has inducible bile acid 7-dehydroxylation activity. At least four new polypeptides were synthesized after addition of primary bile acids to the growth medium. One of these, of molecular weight 27,000 (P-27), was shown to be involved in the 7-de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.5.2070-2077.1988
更新日期:1988-05-01 00:00:00
abstract::Remsen, C. C. (Syracuse University, Syracuse, N.Y.), D. G. Lundgren, and R. A. Slepecky. Inhibition of the development of the spore septum and membranes in Bacillus cereus by beta-phenethyl alcohol. J. Bacteriol. 91:324-331. 1966.-The effect of phenethyl alcohol (PEA) upon the initial stages of sporulation in Bacillus...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.324-331.1966
更新日期:1966-01-01 00:00:00
abstract::FtsZ and ZipA are essential components of the septal ring apparatus, which mediates cell division in Escherichia coli. FtsZ is a cytoplasmic tubulin-like GTPase that forms protofilament-like homopolymers in vitro. In the cell, the protein assembles into a ring structure at the prospective division site early in the di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.18.5153-5166.2000
更新日期:2000-09-01 00:00:00
abstract::sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is formed from an inactive precursor protein (pro-sigma E) by the removal of 27 to 29 amino acids from the pro-sigma E amino terminus. By using oligonucleotide-directed mutagenesis, sequential deletions were constructed in the precursor-specific ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.14.4629-4637.1992
更新日期:1992-07-01 00:00:00
abstract::Population density-dependent expression of luminescence in Vibrio fischeri is controlled by the autoinducer N-3-oxohexanoyl-L-homoserine lactone (autoinducer 1 [AI-1]), which via LuxR activates transcription of the lux operon (luxICDABEG, encoding the putative autoinducer synthase [LuxI] and the luminescence enzymes)....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.4.971-976.1996
更新日期:1996-02-01 00:00:00
abstract::Both Salmonella enterica serovar Typhimurium and Escherichia coli contain the cspH gene encoding CspH, one of the cold shock proteins (CSPs). In this study, we investigated the expression of cspH in S. enterica serovar Typhimurium and found that it was induced in response to a temperature downshift during exponential ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.19.5580-5588.2001
更新日期:2001-10-01 00:00:00
abstract::The type III secretion system (T3SS) of Pseudomonas aeruginosa is an important virulence factor. The T3SS of P. aeruginosa can be induced by a low calcium signal or upon direct contact with the host cells. The exact pathway of signal sensing and T3SS activation is not clear. By screening a transposon insertion mutant ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7575-7585.2004
更新日期:2004-11-01 00:00:00
abstract::Agrobacterium tumefaciens VirD2 polypeptide, in the presence of VirD1, catalyzes a site- and strand-specific nicking reaction at the T-DNA border sequences. VirD2 is found tightly attached to the 5' end of the nicked DNA. The protein-DNA complex is presumably formed via a tyrosine residue of VirD2 (F. Durrenberger, A....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.303-308.1992
更新日期:1992-01-01 00:00:00