ZipA-induced bundling of FtsZ polymers mediated by an interaction between C-terminal domains.

Abstract:

:FtsZ and ZipA are essential components of the septal ring apparatus, which mediates cell division in Escherichia coli. FtsZ is a cytoplasmic tubulin-like GTPase that forms protofilament-like homopolymers in vitro. In the cell, the protein assembles into a ring structure at the prospective division site early in the division cycle, and this marks the first recognized event in the assembly of the septal ring. ZipA is an inner membrane protein which is recruited to the nascent septal ring at a very early stage through a direct interaction with FtsZ. Using affinity blotting and protein localization techniques, we have determined which domain on each protein is both sufficient and required for the interaction between the two proteins in vitro as well as in vivo. The results show that ZipA binds to residues confined to the 20 C-terminal amino acids of FtsZ. The FtsZ binding (FZB) domain of ZipA is significantly larger and encompasses the C-terminal 143 residues of ZipA. Significantly, we find that the FZB domain of ZipA is also required and sufficient to induce dramatic bundling of FtsZ protofilaments in vitro. Consistent with the notion that the ability to bind and bundle FtsZ polymers is essential to the function of ZipA, we find that ZipA derivatives lacking an intact FZB domain fail to support cell division in cells depleted for the native protein. Interestingly, ZipA derivatives which do contain an intact FZB domain but which lack the N-terminal membrane anchor or in which this anchor is replaced with the heterologous anchor of the DjlA protein also fail to rescue ZipA(-) cells. Thus, in addition to the C-terminal FZB domain, the N-terminal domain of ZipA is required for ZipA function. Furthermore, the essential properties of the N domain may be more specific than merely acting as a membrane anchor.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hale CA,Rhee AC,de Boer PA

doi

10.1128/jb.182.18.5153-5166.2000

keywords:

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

5153-66

issue

18

eissn

0021-9193

issn

1098-5530

journal_volume

182

pub_type

杂志文章
  • Proteolytic adaptor for transfer-messenger RNA-tagged proteins from alpha-proteobacteria.

    abstract::We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01387-06

    authors: Lessner FH,Venters BJ,Keiler KC

    更新日期:2007-01-01 00:00:00

  • Homology with a repeated Yersinia pestis DNA sequence IS100 correlates with pesticin sensitivity in Yersinia pseudotuberculosis.

    abstract::We have identified IS100 sequences in a specific subset of Yersinia pseudotuberculosis isolates that were also sensitive to the Y. pestis-produced bacteriocin, pesticin. In contrast, Y. pseudotuberculosis strains which did not contain IS100 sequences were not sensitive to pesticin. We propose that IS100 serves as a mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.6.2081-2085.1997

    authors: McDonough KA,Hare JM

    更新日期:1997-03-01 00:00:00

  • Phase variation of Ag43 is independent of the oxidation state of OxyR.

    abstract::OxyR is a DNA binding protein that differentially regulates a cell's response to hydrogen peroxide-mediated oxidative stress. We previously reported that the reduced form of OxyR is sufficient for repression of transcription of agn43 from unmethylated template DNA, which is essential for deoxyadenosine methylase (Dam)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.7.2203-2209.2003

    authors: Wallecha A,Correnti J,Munster V,van der Woude M

    更新日期:2003-04-01 00:00:00

  • Collaborative regulation of Escherichia coli glutamate-dependent acid resistance by two AraC-like regulators, GadX and GadW (YhiW).

    abstract::An important feature of Escherichia coli pathogenesis is an ability to withstand extremely acidic environments of pH 2 or lower. This acid resistance property contributes to the low infectious dose of pathogenic E. coli species. One very efficient E. coli acid resistance system encompasses two isoforms of glutamate de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.24.7001-7012.2002

    authors: Ma Z,Richard H,Tucker DL,Conway T,Foster JW

    更新日期:2002-12-01 00:00:00

  • Structure of the DNA-SspC complex: implications for DNA packaging, protection, and repair in bacterial spores.

    abstract::Bacterial spores have long been recognized as the sturdiest known life forms on earth, revealing extraordinary resistance to a broad range of environmental assaults. A family of highly conserved spore-specific DNA-binding proteins, termed alpha/beta-type small, acid-soluble spore proteins (SASP), plays a major role in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.11.3525-3530.2004

    authors: Frenkiel-Krispin D,Sack R,Englander J,Shimoni E,Eisenstein M,Bullitt E,Horowitz-Scherer R,Hayes CS,Setlow P,Minsky A,Wolf SG

    更新日期:2004-06-01 00:00:00

  • Naturally occurring Staphylococcus epidermidis plasmid expressing constitutive macrolide-lincosamide-streptogramin B resistance contains a deleted attenuator.

    abstract::A naturally occurring constitutive macrolide-lincosamide-streptogramin B (MLS) resistance plasmid, pNE131, from Staphylococcus epidermidis was chosen to study the molecular basis of constitutive expression. Restriction and functional maps of pNE131 are presented along with the nucleotide sequence of ermM, the gene whi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.2.479-483.1986

    authors: Lampson BC,Parisi JT

    更新日期:1986-05-01 00:00:00

  • Purification and properties of glycogen isolated from a blue-green alga, Nostoc muscorum.

    abstract::Alpha granules were isolated from a blue-green alga, Nostoc muscorum, in large quantities and high purity by sucrose density gradient centrifugation. Sodium deoxycholate was used to eliminate membrane contamination. Isolated alpha granules from this species have average dimension of 31 nm in width and 65 nm in length....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.1.331-338.1971

    authors: Chao L,Bowen CC

    更新日期:1971-01-01 00:00:00

  • Suppressed nonsense mutations in the araC gene of Escherichia coli provide three novel variant proteins.

    abstract::A total of 400 suppressible mutations have been isolated in the araC gene of Escherichia coli. Based on deletion mapping, growth patterns when suppressed, and intragenic recombination, 37 mutants have been determined to contain unique mutations. Rapid plate assays were developed to test for each of the three AraC prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.3.1329-1338.1983

    authors: Schecter JB

    更新日期:1983-06-01 00:00:00

  • Nitric oxide signaling in Pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic di-GMP levels, and enhanced dispersal.

    abstract::Bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state to complete the biofilm life cycle. The signaling molecule nitric oxide (NO) was previously found to trigger biofilm dispersal in the opportunistic pathogen Pseudomonas aeruginosa at low, nontoxic concentrations ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00975-09

    authors: Barraud N,Schleheck D,Klebensberger J,Webb JS,Hassett DJ,Rice SA,Kjelleberg S

    更新日期:2009-12-01 00:00:00

  • Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among.

    abstract::Weiss, Emilio (Naval Medical Research Institute, Bethesda, Md.) and Harry R. Dressler. Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among strains. J. Bacteriol. 83:409-414. 1962.-A further effort to produce genetically labeled Rickettsia pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.409-414.1962

    authors: WEISS E,DRESSLER HR

    更新日期:1962-02-01 00:00:00

  • Genetic analysis of the Sinorhizobium meliloti BacA protein: differential effects of mutations on phenotypes.

    abstract::Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.21.6444-6453.2001

    authors: LeVier K,Walker GC

    更新日期:2001-11-01 00:00:00

  • Utilization of octopine and nopaline by Agrobacterium.

    abstract::Tests for utilization of d-octopine and nopaline in defined media containing a carbon and nitrogen source were made on 60 strains of Agrobacterium representing four species and on a representative of each of five species of Rhizobium. Among 46 virulent strains of Agrobacterium, only two strains were found which utiliz...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.1.378-383.1973

    authors: Lippincott JA,Beiderbeck R,Lippincott BB

    更新日期:1973-10-01 00:00:00

  • Identification of Zoogloea species and the relationship to zoogloeal matrix and floc formation.

    abstract::Three floc-forming, gram-negative, polarly flagellated rods were isolated and characterized. Our isolates were compared to four similar floc-forming organisms previously isolated in another laboratory and classified as two species of Zoogloea, one of Pseudomonas, and as one unidentified gram-negative rod. Possession o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1903-1909.1968

    authors: Friedman BA,Dugan PR

    更新日期:1968-05-01 00:00:00

  • More than Rotating Flagella: Lipopolysaccharide as a Secondary Receptor for Flagellotropic Phage 7-7-1.

    abstract::Bacteriophage 7-7-1, a member of the family Myoviridae, infects the soil bacterium Agrobacterium sp. strain H13-3. Infection requires attachment to actively rotating bacterial flagellar filaments, with flagellar number, length, and rotation speed being important determinants for infection efficiency. To identify the s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00363-18

    authors: Gonzalez F,Helm RF,Broadway KM,Scharf BE

    更新日期:2018-09-10 00:00:00

  • Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins.

    abstract::Type VI secretion systems (T6SS) are bacteriophage-derived macromolecular machines responsible for the release of at least two proteins in the milieu, which are thought to form an extracellular appendage. Although several T6SS have been shown to be involved in the virulence of animal and plant pathogens, clusters enco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00029-11

    authors: Bernard CS,Brunet YR,Gavioli M,Lloubès R,Cascales E

    更新日期:2011-05-01 00:00:00

  • Altered mRNA metabolism in ribonuclease III-deficient strains of Escherichia coli.

    abstract::The metabolism of mRNA from the lactose (lac) operon of Escherichia coli has been studied in ribonuclease (RNase) III-deficient strains (rnc-105). The induction lag for beta-galactosidase from the first gene was twice as long, and enzyme synthesis was reduced 10-fold in one such mutant compared with its isogenic rnc+ ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.2.528-541.1978

    authors: Talkad V,Achord D,Kennell D

    更新日期:1978-08-01 00:00:00

  • The hrpA and hrpC operons of Erwinia amylovora encode components of a type III pathway that secretes harpin.

    abstract::A 6.2-kb region of DNA corresponding to complementation groups II and III of the Erwinia amylovora hrp gene cluster was analyzed. Transposon mutagenesis indicated that the two complementation groups are required for secretion of harpin, an elicitor of the hypersensitive reaction. The sequence of the region revealed 10...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.5.1690-1697.1997

    authors: Kim JF,Wei ZM,Beer SV

    更新日期:1997-03-01 00:00:00

  • Leptospira species categorized by arbitrarily primed polymerase chain reaction (PCR) and by mapped restriction polymorphisms in PCR-amplified rRNA genes.

    abstract::Reference strains from 48 selected serovars representing eight species of Leptospira were examined by two polymerase chain reaction (PCR)-based strategies. First, mapped restriction site polymorphisms (MRSP) were examined in PCR products from portions of rrs (16S rRNA gene) and rrl (23S rRNA gene). Twenty MRSP and 2 l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.4.973-981.1993

    authors: Ralph D,McClelland M,Welsh J,Baranton G,Perolat P

    更新日期:1993-02-01 00:00:00

  • Genetic and phenotypic analyses indicating occurrence of the recN262 and radB101 mutations at the same locus in Escherichia coli.

    abstract::The radB101 and recN262 mutations showed essentially identical phenotypes when compared in isogenic Escherichia coli strains for their effects on gamma and UV radiation survival and on conjugal recombination in a uvrA recB recC sbcB sbcC strain. Complementation tests involving attempts to reconstitute a radB+ recN+ st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.5.2392-2394.1988

    authors: Sargentini NJ,Smith KC

    更新日期:1988-05-01 00:00:00

  • Comparison of the membrane composition of Spiroplasma citri and the corn stunt Spiroplasma.

    abstract::Components of membranes isolated from Spiroplasma citri and corn stunt spiroplasma grown at 28 degrees C were analyzed. On a protein basis, lipid phosphorus was lower and cholesterol was higher in S. citri. Only minor differences between the two species were found in fatty acid composition, reduced nicotinamide adenin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.2.1056-1058.1979

    authors: Mudd JB,Lee IM,Liu HY,Calavan EC

    更新日期:1979-02-01 00:00:00

  • VirG, a Yersinia enterocolitica lipoprotein involved in Ca2+ dependency, is related to exsB of Pseudomonas aeruginosa.

    abstract::Pathogenic yersiniae require Ca2+ for growth at 37 degrees C. They harbor closely related plasmids of about 70 kb that are essential for virulence. At 37 degrees C and in the absence of Ca2+ ions, these plasmids cause a decrease in growth rate and the release of large amounts of proteins called Yops. Here we describe ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.15.4230-4237.1995

    authors: Allaoui A,Scheen R,Lambert de Rouvroit C,Cornelis GR

    更新日期:1995-08-01 00:00:00

  • Cloning and characterization of the gene cluster for palatinose metabolism from the phytopathogenic bacterium Erwinia rhapontici.

    abstract::Erwinia rhapontici is able to convert sucrose into isomaltulose (palatinose, 6-O-alpha-D-glucopyranosyl-D-fructose) and trehalulose (1-O-alpha-D-glucopyranosyl-D-fructose) by the activity of a sucrose isomerase. These sucrose isomers cannot be metabolized by plant cells and most other organisms and therefore are possi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.8.2425-2430.2001

    authors: Börnke F,Hajirezaei M,Sonnewald U

    更新日期:2001-04-01 00:00:00

  • Genetic analysis of antibiotic resistance in Streptococcus pyogenes.

    abstract::The genetics of antibiotic resistance in mutant strains of Streptococcus pyrogenes was studied. Utilizing a type 6 strain (9440) primarily resistant to strepttomycin (Strr), classes of mutant strains were isolated that were resistant to one of the following antibiotics: rifampin (Rifr), erythromycin (Eryr), thiostrept...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.852-859.1978

    authors: Stuart JG,Ferretti JJ

    更新日期:1978-02-01 00:00:00

  • Truncation analysis of TatA and TatB defines the minimal functional units required for protein translocation.

    abstract::The TatA and TatB proteins are essential components of the twin arginine protein translocation pathway in Escherichia coli. C-terminal truncation analysis of the TatA protein revealed that a plasmid-expressed TatA protein shortened by 40 amino acids is still fully competent to support protein translocation. Similar tr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.21.5871-5879.2002

    authors: Lee PA,Buchanan G,Stanley NR,Berks BC,Palmer T

    更新日期:2002-11-01 00:00:00

  • Whole-genome sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H.

    abstract::We present the genomic sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H (ATCC 43290), a clinical isolate from the Colorado Department of Health, Denver, CO. This is the first example of a genome sequence of L. pneumophila from a serogroup other than serogroup 1. We highlight the simil...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06626-11

    authors: Amaro F,Gilbert JA,Owens S,Trimble W,Shuman HA

    更新日期:2012-03-01 00:00:00

  • Cyanide-resistant respiration of a Neurospora crassa membrane mutant.

    abstract::Choline starvation of the Neurospora crassa chol-1 mutant leads to a decrease in respiration through the cytochrome chain and a concomitant induction of the alternate oxidase. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.1.542-543.1976

    authors: Juretic D

    更新日期:1976-04-01 00:00:00

  • Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Mycobacterium tuberculosis.

    abstract::Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Myobacterium tuberculosis. J. Bacteriol. 91:2146-2154. 1966.-The ribosomal fraction of the attenuated strain, H37R...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.6.2146-2154.1966

    authors: Youmans AS,Youmans GP

    更新日期:1966-06-01 00:00:00

  • Topological model of the Rhodobacter capsulatus light-harvesting complex I assembly protein LhaA (previously known as ORF1696).

    abstract::A theoretical topology of the Rhodobacter capsulatus membrane protein LhaA was formulated and evaluated by gene fusion experiments. The apparent topological locations of fusion enzymes were compared with the theoretically derived structure, and a model of LhaA is suggested that consists of 12 transmembrane segments, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4742-4745.1998

    authors: Young CS,Beatty JT

    更新日期:1998-09-01 00:00:00

  • Characterization of the rfc region of Shigella flexneri.

    abstract::The O antigen of the Shigella flexneri lipopolysaccharide (LPS) is an important virulence determinant and immunogen. We have isolated S. flexneri mutants which produce a semi-rough LPS by using an O-antigen-specific phage, Sf6c. Western immunoblotting was used to show that the LPS produced by the semi-rough mutants co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.3.733-747.1994

    authors: Morona R,Mavris M,Fallarino A,Manning PA

    更新日期:1994-02-01 00:00:00

  • rec-2-dependent phage recombination in Haemophilus influenzae.

    abstract::The genetic transformation mutant Rd(DB117)rec- has a pleiotropic phenotype that includes reduced levels of phage recombination. Physical mapping experiments showed that this strain has a 78.5-kbp insertion in the rec-2 gene. The rec-2 dependence of phage recombination was reexamined to determine whether the defective...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.15.4960-4966.1992

    authors: Kupfer DM,McCarthy D

    更新日期:1992-08-01 00:00:00